fa معرفی سه نماتد انگل گیاهی تیره Longidoridae از باغهای شهرستان رفسنجان، ایران تخصصي Special پژوهشي Research <span style="font-size:11pt"><span style="line-height:normal"><span style="tab-stops:117.0pt center 234.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">شریفی رستم </span></span><span lang="AR-SA" style="font-size:14.0pt">&shy;</span><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">آبادی</span></span><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:"> س، رستمی م، جهانشاهی افشار ف، اردشیر ف (1401) معرفی سه نماتد انگل گیاهی تیره </span></span><i><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Longidoridae</span></span></i> <span style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">&nbsp;از باغهای شهرستان رفسنجان، ایران. </span></span><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">دانش بیماری‌شناسی گیاهی 12(1):&nbsp; 12</span></span><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">-</span></span><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">24.&nbsp;&nbsp;&nbsp;</span></span></span></span></span></span></span></span> <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">مقدمه</span></span></b><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">: نماتدهای انگل گیاهی تیره </span></span><i><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Longidoridae</span></span></i><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">، از راسته‌ی </span></span><i><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Dorilaimida</span></span></i><span style="font-size:14.0pt"><span b="" nazanin="" style="font-family:"> ، می‌توانند هم به‌طور مستقیم و هم به‌واسطه انتقال بعضی ویروسها به بسیاری از محصولهای کشاورزی و به‌ویژه درختان میوه خسارت وارد نمایند. با توجه به نبود پژوهشهای قبلی در این زمینه در باغهای میوه منطقه رفسنجان، شناسایی و پایش جمعیت آن‌ها حائز اهمیت است. <b>مواد و روش‌ها:</b> برای شناسایی نماتدهای انگل محصولهای باغی در شهرستان رفسنجان، تعدادی نمونه </span></span><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">خاک و ریشه از</span></span> <span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">اطراف</span></span> <span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">ریشه</span></span> <span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">انواع </span></span><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">درختان میوه</span></span> <span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">از</span></span> <span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">مناطق</span></span> <span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">مختلف</span></span> <span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">شهرستان</span></span> <span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">در سال 1399</span></span> <span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">جمع</span></span> <span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">آوری</span></span> <span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">گردید.</span></span> <span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">نماتدها به دو روش سینی و الک-سانتریفیوژ</span></span> <span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">استخراج</span></span> <span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">شده</span></span> <span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">و پس</span></span> <span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">از</span></span> <span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">تثبیت</span></span> <span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">به</span></span> <span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">گلیسرین</span></span> <span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">خالص</span></span> <span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">منتقل</span></span> <span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">شدند. پس از تهیه اسلایدهای دائم، نمونه&shy; ها با میکروسکوپ نوری و با مراجعه به منابع معتبر</span></span><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">،</span></span><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:"> مطالعه و در سطح گونه مورد شناسایی قرار گرفتند</span></span><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">.</span></span> <b><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">یافته‌ها:</span></span></b><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:"> سه گونه‌ی </span></span><i><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Longidorus africanus</span></span></i><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">، </span></span><i><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Xiphinema vuittenezi</span></span></i><i> </i><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">و </span></span><i><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">X. index</span></span></i><i> </i><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">از خاک اطراف ریشه در این مطالعه جدا شدند.</span></span> <span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">همچنین </span></span><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">به منظور شناسایی تکمیلی،</span></span> <i><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">X. vuittenezi</span></span></i> <span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">با تکثیر ناحیه </span></span><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">D2-D3</span></span> <span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">(LSU rDNA)</span></span><span style="font-size:14.0pt"><span b="" nazanin="" style="font-family:"> مورد مطالعه مولکولی نیز قرارگرفت. </span></span><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">توالی به‌دست‌آمده 100 درصد با توالی‌های سایر جمعیت‌های </span></span><i><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">X. vuittenezi</span></span></i><span style="font-size:14.0pt"><span b="" nazanin="" style="font-family:"> در بانک ژن یکسان بود. </span></span><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:"><b>نتیجه &shy;گیری:</b> این پژوهش نشان می&shy;دهد پایش جمعیت نماتدهای منطقه نیاز به توجه بیشتری دارد.</span></span><span lang="FA" style="font-size:14.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></span></div> <br> &nbsp; <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Sharify Rostam-Abadi, S., Rostami, M., Jahanshahi Afshar, F., Ardeshir, F. (2023). &nbsp;Three plant-parasitic nematodes of the family <i>Longidoridae</i> from orchards of Rafsanjan county, Iran. <i>Plant Pathology Science</i> <i>12(1),12-24.</i>&nbsp;&nbsp; </span></span></span></span></span></div> &nbsp;<span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Introduction: </span></span></b><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Longidoridae</span></span></i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> is a family of plant-parasitic nematodes belonging to the order <i>Dorylaimida</i>, that cause damage to most agricultural crops especially fruit trees by direct damage and also transmitting some plant pathogenic viruses. Considering the lack of prior research studies on this topic in the fruit orchards of Rafsanjan region, identifying and monitoring their population is important.<b> Materials and Methods: </b>In a study that was conducted to identify plant-parasitic nematodes of horticultural products in Rafsanjan city, some soil samples were collected from the rhizosphere of various fruit trees, in 2020. The nematodes were extracted from the soil samples using two methods, centrifugal flotation-sieving and tray technique, fixed and transferred to the anhydrous glycerin. After preparing permanent slides, the nematodes were studied using a light microscopy. The nematode species were identified based upon morphological and morphometric data using relevant valid references.<b> Results: </b>In this study, three species of <i>Dorylaimida</i> order belonging to the <i>Longidoridae</i> family including the two genera: <i>Longidorus</i> (<i>L. africanus</i>) and <i>Xiphinema</i> (<i>X. vuittenezi </i>and <i>X. index</i>) were obtained from the rhizosphere. Also, in order to confirm the traditional identification of <i>X. vuittenezi</i>, this species was molecularly studied using D2-D3 extension fragment of LSU rDNA. The obtained sequence of the species was 100% identical to the sequences of the other populations of <i>X. vuittenezi</i> in the GenBank. <b>Conclusion:</b> This study shows that regional nematode population monitoring needs more attention.</span></span></span></span></span></span><br type="_moz" > <br> <span style="font-size:11pt"><span style="line-height:normal"><span style="text-autospace:none"><span calibri="" style="font-family:"><span style="font-size:12.0pt"><span style="background:#fcfcfc"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><span style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif"></span></span></span></span></span><br> &nbsp; انار, هلو, Longidorus, Xiphinema Longidorus, Peach, Pomegranate, Xiphinema 12 24 http://yujs.yu.ac.ir/pps/browse.php?a_code=A-10-418-1&slc_lang=fa&sid=1 saeed Sharify Rostam-Abady سعید شریفی رستم آبادی saeed.sharify.1793@gmail.com 10031947532846005367 10031947532846005367 No Department of Plant Pathology, Rafsanjan Branch, Islamic Azad University, Rafsanjan, Iran گروه بیماری شناسی گیاهی، واحد رفسنجان، دانشگاه آزاد اسلامی، رفسنجان، ایران Mahdieh Rostami مهدیه رستمی m.rostami_1355@yahoo.com 10031947532846005368 10031947532846005368 Yes Department of Plant Pathology, Rafsanjan Branch, Islamic Azad University, Rafsanjan, Iran گروه بیماری شناسی گیاهی، واحد رفسنجان، دانشگاه آزاد اسلامی، رفسنجان، ایران Farahnaz Jahanshahi Afshar فرحناز جهانشاهی افشار afshar_fj@yahoo.com 10031947532846005369 10031947532846005369 No Research Assistant Professor, Agricultural Zoology Research Department, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization, Ministry of Agriculture-Jahad, Tehran, Iran استادیار پژوهشی، بخش تحقیقات جانورشناسی کشاورزی، مؤسسه تحقیقات گیاه‌پزشکی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، وزارت جهاد کشاورزی، تهران، ایران Fariba Ardeshir فریبا اردشیر 10031947532846005370 10031947532846005370 No Research Assistant Professor, Agricultural Zoology Research Department, Iranian Research Institute of Plant Protection, Agricultural Research, Education and Extension Organization, Ministry of Agriculture-Jahad, Tehran, Iran استادیار پژوهشی، بخش تحقیقات جانورشناسی کشاورزی، مؤسسه تحقیقات گیاه‌پزشکی کشور، سازمان تحقیقات، آموزش و ترویج کشاورزی، وزارت جهاد کشاورزی، تهران، ایران