fa واکنش 32 ژنوتیپ گوجه فرنگی به نماتد غده ریشه Meloidogyne javanica تخصصي Special پژوهشي Research <span style="font-size:11pt"><span style="line-height:normal"><span style="tab-stops:117.0pt center 234.0pt"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">ابوترابی ا (1401) واکنش 32 ژنوتیپ گوجه &shy;فرنگی به نماتد غده ریشه&shy;</span></span><i> </i><i><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Meloidogyne javanica</span></span></i><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">.<b> </b></span></span><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">دانش بیماری‌شناسی گیاهی 12(1): </span></span><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">25-35.</span></span>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</span></span></span></span></span></span> <div dir="ltr" style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:">&nbsp;</span></span></span></span></span><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">مقدمه:</span></span></b> <span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">این پژوهش با هدف تعیین واکنش</span></span><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:"> 32 </span></span><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">ژنوتیپ گوجه&shy; فرنگی از بانک ژن گیاهی ایران به نماتد غده ریشه&shy;</span></span><i> </i><i><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Meloidogyne javanica</span></span></i><span style="font-size:14.0pt"><span b="" nazanin="" style="font-family:"> ، برای شناسایی ژنوتیپ&shy;های مقاوم طی دو سال در شرایط گلخانه انجام شد. </span></span><b><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">مواد و روش&shy;ها:</span></span></b> <span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">پس از خالص&shy;سازی جمعیت نماتد و کاشت بذرهای </span></span><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">32 </span></span><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">ژنوتیپ </span></span></span></span></span></span></span></span></span><span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">گوجه&shy; فرنگی و رسیدن نشاها به مرحله 4-3 برگی، هر تیمار </span></span><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">در قالب پنج تکرار </span></span><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">با&nbsp; 6000 تخم و لارو </span></span><i><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">M. javanica</span></span></i><span style="font-size:14.0pt"><span b="" nazanin="" style="font-family:"> ، تلقیح گردید و 60 روز پس از آلوده&shy; سازی، صفات رویشی و صفات وابسته به جمعیت نماتد در هر تیمار تعیین شد و بر&shy;اساس شاخص گال (</span></span><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">GI</span></span><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">) و فاکتور تولیدمثلی نماتد (</span></span><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Rf</span></span><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">)، واکنش ژنوتیپ&shy;ها نسبت به نماتد، تعیین گردید</span></span><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">.</span></span> <b><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">یافته&shy; ها: </span></span></b><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">هیجده ژنوتیپ با </span></span><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&nbsp;Rf>1</span></span><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:"> و</span></span><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">GI>2 </span></span><span style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">&nbsp;حساس، 23 ژنوتیپ با </span></span><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&nbsp;Rf<1</span></span><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">و </span></span><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&nbsp;GI>2 </span></span><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">نیمه&shy; مقاوم، و سه ژنوتیپ </span></span><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">TN-72-938</span></span><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">، </span></span><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">TN-72-1041</span></span><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:"> و</span></span></span></span></span></span></span></span></span></div> <span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">TN-72-1012</span></span><span style="font-size:14.0pt"><span b="" nazanin="" style="font-family:"> با</span></span><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&le;1&nbsp; </span></span>&nbsp;<span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Rf</span></span><span style="font-size:14.0pt"><span b="" nazanin="" style="font-family:"> و </span></span><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">GI&le;2</span></span><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">&nbsp; مقاوم به این نماتد مخرب شناسایی</span></span> <span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">شدند</span></span><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">. </span></span><b><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">نتیجه &shy;گیری: </span></span></b><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">از این سه ژنوتیپ مقاوم می&shy;توان در برنامه مدیریت </span></span><i><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">M. javanica</span></span></i><span style="font-size:14.0pt"><span b="" nazanin="" style="font-family:"> استفاده کرد.</span></span><span lang="FA" style="font-size:14.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:">&nbsp;</span></span></span></span></span><br> &nbsp; <div style="text-align: justify;"><span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span calibri="" style="font-family:"><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Abootorabi E (2023) The reaction of 32 tomato genotypes to root-knot nematode </span></span><i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Meloidogyne javanica</span></span></i><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">. Plant Pathology Science, 12(1):25-35.&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span></span></span></span></span></div> <div style="text-align: justify;">&nbsp;<span style="font-size:11pt"><span style="line-height:normal"><span calibri="" style="font-family:"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Introduction: </span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">This research was conducted with the aim of determining the reaction of 32 tomato genotypes from the plant gene bank of Iran to the root-knot nematode <i>Meloidogyne javanica</i>, to identify resistant genotypes during two years under greenhouse conditions.<b> Materials and Methods: </b>After purifying the nematode population and planting the seeds of 32 tomato genotypes and reaching the stage of 3-4 leaves, each treatment was inoculated with 6000 eggs and larvae of <i>M. javanica</i> in five replicates, and 60 days after inoculation, vegetative traits and traits related to nematode population were determined in each treatment, and based on gall index (GI) and nematode reproductive factor (Rf), the reaction of genotypes to nematode was determined.<b> Results: </b>Eighteen&nbsp; genotypes with Rf>1 and GI>2, 23 genotypes with Rf<1 and GI>2, and three genotypes TN-72-938, TN-72-1041 and TN-72-1012 with Rf&le;1 and GI&le;2, were identified as susceptible, moderately resistant, and resistant to this destructive nematode respectively. <b>Conclusion: </b>These three resistant genotypes can be used in the <i>M. javanica</i> management program.</span></span></span></span></span></div> <br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span lang="FA" style="font-size:14.0pt"><span style="font-family:"B Nazanin""></span></span></b></span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span calibri="" style="font-family:"><b><span dir="LTR" style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif"></span></span></b></span></span></span></span></span><br> &nbsp; شاخص گال, فاکتور تولیدمثل, حساس, مقاوم Gall index, Reproductive factor, Susceptible, Resistant 25 35 http://yujs.yu.ac.ir/pps/browse.php?a_code=A-10-254-3&slc_lang=fa&sid=1 Elmira Abootorabi المیرا ابوترابی elabootorabi@gmail.com 10031947532846005371 10031947532846005371 Yes Department of Nematology, Iranian Research Institute of Plant Protection, Tehran, Iran بخش تحقیقات نماتدشناسی، موسسه تحقیقات گیاه‌پزشکی کشور، تهران