fa جایگاه نسب شناسی جدایه استان لرستان ایران ویروس برگ بادبزنی انگور تخصصي Special پژوهشي Research <div><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">&nbsp;پاکباز س، </span></span><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">درویش‌نیا م، نقوی ا (1400)</span></span> <span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">جایگاه نسب&shy; شناسی جدایه استان لرستان ایران ویروس برگ بادبزنی انگور. دانش بیماری‌شناسی گیاهی 11(1):12-1.</span></span>&nbsp; &nbsp; &nbsp; &nbsp;</span></span></span></span></span></div> <div dir="ltr"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif">&nbsp; &nbsp; &nbsp;<span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">10.2982/PPS.11.1.1</span></span>&nbsp;<span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Doi: </span></span><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&nbsp;&nbsp; </span></span></span></span></span></span></span></div> <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">مقدمه:</span></span></b><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:"> ویروس</span></span> <span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">برگ</span></span> <span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">بادبزنی</span></span><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">(GFLV) </span></span><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">یک ویروس‌ مهم آلوده کننده انگور در جهان است.&nbsp;</span></span></span></span></span></span></span><span lang="AR-SA" style="font-family: Calibri, sans-serif; text-align: justify; font-size: 14pt;"><span b="" nazanin="" style="font-family:"><b>مواد و روش‌ها:</b> </span></span><span lang="FA" style="font-family: Calibri, sans-serif; text-align: justify; font-size: 14pt;"><span b="" nazanin="" style="font-family:">انگورهای دارای نشانه </span></span><span dir="LTR" style="font-family: Calibri, sans-serif; text-align: justify; font-size: 12pt;"><span new="" roman="" style="font-family:" times="">GFLV</span></span><span lang="FA" style="font-family: Calibri, sans-serif; text-align: justify; font-size: 14pt;"><span b="" nazanin="" style="font-family:"> از حومه شهر خرم‌آباد در استان لرستان ایران،&nbsp; در بهار 1399 نمونه‌برداری شدند و آزمون </span></span><span dir="LTR" style="font-family: Calibri, sans-serif; text-align: justify; font-size: 12pt;"><span new="" roman="" style="font-family:" times="">RT-PCR</span></span><span lang="FA" style="font-family: Calibri, sans-serif; text-align: justify; font-size: 14pt;"><span b="" nazanin="" style="font-family:"> جهت تکثیر ژن </span></span><span dir="LTR" style="font-family: Calibri, sans-serif; text-align: justify; font-size: 12pt;"><span new="" roman="" style="font-family:" times="">GFLV-CP</span></span><span lang="FA" style="font-family: Calibri, sans-serif; text-align: justify; font-size: 14pt;"><span b="" nazanin="" style="font-family:"> آنها انجام و محصول واکنش توالی‌یابی شد. <b>یافته‌ها:</b> آغازگرهای اختصاصی توانستند قطعه‌ای به طول </span></span><span dir="LTR" style="font-family: Calibri, sans-serif; text-align: justify; font-size: 12pt;"><span new="" roman="" style="font-family:" times="">bp</span></span><span lang="FA" style="font-family: Calibri, sans-serif; text-align: justify; font-size: 14pt;"><span b="" nazanin="" style="font-family:"> 1515 مربوط به ژن </span></span><span dir="LTR" style="font-family: Calibri, sans-serif; text-align: justify; font-size: 12pt;"><span new="" roman="" style="font-family:" times="">CP</span></span><span lang="FA" style="font-family: Calibri, sans-serif; text-align: justify; font-size: 14pt;"><span b="" nazanin="" style="font-family:"> را تکثیر کنند. بر اساس توالی نوکلئوتیدی این قطعه، </span></span><span dir="LTR" style="font-family: Calibri, sans-serif; text-align: justify; font-size: 12pt;"><span new="" roman="" style="font-family:" times="">GFLV</span></span><span lang="FA" style="font-family: Calibri, sans-serif; text-align: justify; font-size: 14pt;"><span b="" nazanin="" style="font-family:"> برای نخستین بار در این منطقه شناسایی شد. تشابه ترادف نوکلئوتیدی این جدایه به میزان 64/95 -14/89 درصد با دیگر جدایه‌های موجود در بانک ژن </span></span><span dir="LTR" style="font-family: Calibri, sans-serif; text-align: justify; font-size: 12pt;"><span new="" roman="" style="font-family:" times="">NCBI</span></span><span lang="FA" style="font-family: Calibri, sans-serif; text-align: justify; font-size: 14pt;"><span b="" nazanin="" style="font-family:"> تشخیص داده شد. همچنین در درخت نسب&shy; شناسی&nbsp; این جدایه &shy;ها بر اساس ناحیه ژنومی </span></span><span dir="LTR" style="font-family: Calibri, sans-serif; text-align: justify; font-size: 12pt;"><span new="" roman="" style="font-family:" times="">CP</span></span><span lang="FA" style="font-family: Calibri, sans-serif; text-align: justify; font-size: 14pt;"><span b="" nazanin="" style="font-family:">، جدایه‌های </span></span><span dir="LTR" style="font-family: Calibri, sans-serif; text-align: justify; font-size: 12pt;"><span new="" roman="" style="font-family:" times="">GFLV</span></span><span lang="FA" style="font-family: Calibri, sans-serif; text-align: justify; font-size: 14pt;"><span b="" nazanin="" style="font-family:"> در دو گروه </span></span><span dir="LTR" style="font-family: Calibri, sans-serif; text-align: justify; font-size: 12pt;"><span new="" roman="" style="font-family:" times="">I</span></span><span lang="FA" style="font-family: Calibri, sans-serif; text-align: justify; font-size: 14pt;"><span b="" nazanin="" style="font-family:"> و </span></span><span dir="LTR" style="font-family: Calibri, sans-serif; text-align: justify; font-size: 12pt;"><span new="" roman="" style="font-family:" times="">II</span></span><span lang="FA" style="font-family: Calibri, sans-serif; text-align: justify; font-size: 14pt;"><span b="" nazanin="" style="font-family:"> خوشه‌بندی شدند. جدایه لرستان </span></span><span dir="LTR" style="font-family: Calibri, sans-serif; text-align: justify; font-size: 12pt;"><span new="" roman="" style="font-family:" times="">GFLV</span></span><span lang="FA" style="font-family: Calibri, sans-serif; text-align: justify; font-size: 14pt;"><span b="" nazanin="" style="font-family:"> همراه با جدایه‌های </span></span><span dir="LTR" style="font-family: Calibri, sans-serif; text-align: justify; font-size: 12pt;"><span new="" roman="" style="font-family:" times="">GFLV</span></span><span lang="FA" style="font-family: Calibri, sans-serif; text-align: justify; font-size: 14pt;"><span b="" nazanin="" style="font-family:"> متعلق به ناحیه شمال غربی کشور و جدایه شهر تاکستان در یک زیرشاخه در گروه </span></span><span dir="LTR" style="font-family: Calibri, sans-serif; text-align: justify; font-size: 12pt;"><span new="" roman="" style="font-family:" times="">I</span></span><span lang="FA" style="font-family: Calibri, sans-serif; text-align: justify; font-size: 14pt;"><span b="" nazanin="" style="font-family:"> قرار گرفتند و جدایه‌های مربوط به سایر کشورها نیز در این گروه در یک زیرشاخه مجزا قرار گرفتند. همچنین جدایه‌های شرق کشور و استان‌های فارس و کهگیلویه و بویراحمد نیز در گروه </span></span><span dir="LTR" style="font-family: Calibri, sans-serif; text-align: justify; font-size: 12pt;"><span new="" roman="" style="font-family:" times="">II</span></span><span lang="FA" style="font-family: Calibri, sans-serif; text-align: justify; font-size: 14pt;"><span b="" nazanin="" style="font-family:"> و در زیرشاخه‌های متفاوت قرار گرفتند. <b>نتیجه‌گیری:</b> یافته&shy; های این پژوهش‌ بیانگر بومی بودن ویروس، خاستگاه احتمالی آن از ایران و سپس گسترش آن به سایر نقاط دنیا و نیز اثر جدایی جغرافیایی در تکامل </span></span><span dir="LTR" style="font-family: Calibri, sans-serif; text-align: justify; font-size: 12pt;"><span new="" roman="" style="font-family:" times="">GFLV</span></span><span style="font-family: Calibri, sans-serif; text-align: justify; font-size: 14pt;"><span b="" nazanin="" style="font-family:"> می‌باشد.</span></span></div> <br> &nbsp; <span style="font-size:11pt"><span style="text-justify:kashida"><span style="text-kashida:0%"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Pakbaz S, Darvishnia M, </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Naghavi A (2022)</span></span> <span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Phylogenetic status of <i>Grapevine fanleaf virus</i> isolate of Lorestan province of Iran. </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Plant Pathology Science 11(1):1-12. &nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;Doi: 10.2982/PPS.11.1.1</span></span></span></span></span></span></span></span><br> &nbsp; <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Introduction:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> <i>Grapevine fanleaf virus</i> (GFLV) is a major grapevine infecting virus in the world. <b>Materials and Methods:</b> Grapes showing GFLV signs were sampled from the suburbs of the city of Khorramabad in Lorestan Province of Iran in spring 2020 and RT-PCR test was performed to amplify their GFLV-CP gene and the product was sequenced.<b> Results:</b> Specific primers were able to amplify a 1515 bp fragment of the CP gene. Based on the nucleotide sequence of this fragment, GFLV was first identified in this region. The nucleotide sequence similarity of this isolate was detected at 89.14-95.64% with other isolates in the NCBI library. Also, the phylogenetic tree of these isolates, based on the genomic CP region, grouped GFLV isolates into two groups I and II. The Lorestan GFLV isolate was placed in a subgroup in Group I together with GFLV isolates from the northwestern part of Iran and the Takestan isolate, and isolates from other countries were grouped in a separate subgroup of this group. </span></span><span lang="EN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Also in the </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">phylogenetic tree</span></span><span lang="EN" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">, the Northeast isolates and the Fars and Kohgiloyeh & Boyer-Ahmad province isolates were classified in Group II.<b> </b></span></span><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Conclusion:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> The results of this research indicate that the virus is endemic and that its likely origin was in Iran and then spread to other parts of the world. The impact of geographic segregation on the evolution of GFLV can also be deduced.</span></span></span></span></span></span></div> <br> &nbsp;  توالی‌یابی, GFLV, RT-PCR  GFLV, RT-PCR, Sequencing 1 12 http://yujs.yu.ac.ir/pps/browse.php?a_code=A-10-349-2&slc_lang=fa&sid=1 Samira Pakbaz سمیرا پاکباز samira.pakbaz@gmail.com 10031947532846004760 10031947532846004760 Yes Department of Plant Protection, Faculty of Agriculture, Lorestan University, Khorramabad, Iran گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه لرستان، خرم‌آّباد Mostafa Darvishnia مصطفی درویش نیا mdarvishnia44@yahoo.com 10031947532846004761 10031947532846004761 No Department of Plant Protection, Faculty of Agriculture, Lorestan University, Khorramabad, Iran گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه لرستان، خرم‌آّباد Arezoo Naghavi آرزو نقوی ar-ng11@yahoo.com 10031947532846004762 10031947532846004762 No Department of Plant Protection, Faculty of Agriculture, Lorestan University, Khorramabad, Iran گروه گیاه‌پزشکی، دانشکده کشاورزی، دانشگاه لرستان، خرم‌آّباد