fa 8- معرفی سه نماتد بیمارگر حشرات بومی ایران و تاثیر آنها بر شب پره موم خوار زنبورعسل تخصصي Special پژوهشي Research <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">ابوترابی </span></span><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">&nbsp;ا، </span></span><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">ابراهیمی </span></span><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">&nbsp;ل (1400) </span></span><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">معرفی سه نماتد بیمارگر حشرات بومی ایران و تاثیر آنها بر شب&shy; پره موم &shy;خوار زنبورعسل</span></span><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">. دانش بیماری&shy;شناسی گیاهی 11(1):</span></span> <span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">99</span></span><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">-89.</span></span> <span lang="FA" style="font-size:14.0pt"><span style="font-family:"B Nazanin""></span></span></span></span></span></span></span></div> <div dir="ltr" style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif">&nbsp; <span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Doi: &nbsp;10.2982/PPS.11.1.89.&nbsp;</span></span></span></span></span></span></span></div> <div style="text-align: justify;"><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="direction:rtl"><span style="unicode-bidi:embed"><span style="font-family:Calibri,sans-serif"><b><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">مقدمه:</span></span></b><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:"> این پژوهش با هدف جمع آوری و شناسایی نماتدهای بیمارگر حشرات بومی ایران و ارزیابی قدرت بیمارگری آنها روی لارو شب&shy; پره موم‌خوار زنبورعسل (</span></span><i><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Galleria mellonella</span></span></i><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">)، اجرا شد. <b>مواد و روش‌ها:</b> سیزده جدایه نماتدهای بیمارگر حشرات از استانهای مختلف ایران جمع &shy;آوری و بر اساس صفات ریختی، شناسایی شدند. درصد مرگ‌ومیر لارو </span></span><i><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">G. mellonella</span></span></i><i> </i><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">آلوده شده به این جدایه‌ها در دمای 1</span></span><span lang="AR-SA" style="font-size:14.0pt"><span cambria="" style="font-family:">&plusmn;</span></span><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">25 و 1</span></span><span lang="AR-SA" style="font-size:14.0pt"><span cambria="" style="font-family:">&plusmn;</span></span><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">32 درجه سلسیوس در آزمایش یک به روی یک و &nbsp;هدف‌یابی جدایه‌ها و درصد تلفات آفت در آزمایش ستون شنی، تعیین شدند. <b>یافته‌ها: </b>هفت جدایه نماتدها&nbsp; </span></span><i><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Heterorhabditis bacteriophora</span></span></i><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">، دو جدایه </span></span>&nbsp;<i><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Steinernema feltiae </span></span></i><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">&nbsp;و پنج جدایه </span></span><i><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Steinernema carpocapsae</span></span></i><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:"> شناسایی شدند. توانایی نفوذ جدایه‌های هر سه گونه نماتد به بدن حشره، ظرف مدت 72-48 ساعت پس از آلودگی در دمای 1</span></span><span lang="AR-SA" style="font-size:14.0pt"><span cambria="" style="font-family:">&plusmn;</span></span><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">25 درجه سلسیوس و بیمارگری جدایه‌ها، تا 93 درصد ثابت شد. دمای بهینه برای فعالیت زیستی جدایه‌های شناسایی شده، 1</span></span><span lang="AR-SA" style="font-size:14.0pt"><span cambria="" style="font-family:">&plusmn;</span></span><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">25 درجه سلسیوس بود. سرعت هدف‌یابی گونه </span></span><i><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">S. carpocapsae</span></span></i><span lang="AR-SA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:"> در مقایسه با دو گونه دیگر در آزمایش ستون شنی، بیشتر بود.</span></span> <b><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">نتیجه‌گیری: </span></span></b><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">جدایه‌های </span></span><i><span dir="LTR" style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">S. carpocapsae</span></span></i><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:"> نسبت به دو گونه دیگر نماتد </span></span><span lang="FA" style="font-size:14.0pt"><span b="" nazanin="" style="font-family:">توان بالاتری در هدف‌یابی و بیمارگری لارو شب&shy; پره موم &shy;خوار زنبورعسل دارند.</span></span></span></span></span></span></span><br> &nbsp;</div> <span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Abootorabi E, Ebrahimi L</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> (2022)</span></span><b> </b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Introduction of three entomopathogenic nematodes </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">of Iran</span></span><b> </b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">and their impact on honeycomb moth</span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">. </span></span><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Plant Pathology Science 11(1):89-99.&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp; </span></span></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">&nbsp;Doi: 10.2982/PPS.11.1.89.</span></span></span></span></span><br> &nbsp; <div style="text-align: justify;"><span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times="">Introduction:</span></span></b><span style="font-size:12.0pt"><span new="" roman="" style="font-family:" times=""> The aim of this study was to collect and identify entomopathogenic nematodes native to Iran and to evaluate their pathogenicity on honeycomb moth larvae (<i>Galleria mellonella</i>). <b>Materials and Methods:</b> Thirteen isolates of entomopathogenic nematodes were collected from different provinces of Iran and identified based on morphological characters. The percentage mortality of <i>G. mellonella</i> larvae infected with these isolates at 25 &plusmn; 1 and 32 &plusmn; 1 &deg;C was determined in a one-to-one assay, and the ability of the isolates to find a target and the mortality of the insect in the sand column test were determined. <b>Results:</b> Seven isolates of <i>Heterorhabditis</i> <i>bacteriophora</i>, two isolates of <i>Steinernema</i> <i>feltiae</i>, and five isolates of <i>S.</i> <i>carpocapsae</i> were identified. The ability of isolates of all three nematode species to penetrate the insect&#39;s body has been shown to be up to 93% within 48-72 hours post-infection at 25 &plusmn; 1&deg;C. The optimum temperature for the biological activity of the identified isolates was 25&plusmn;1&deg;C. <i>S. carpocapsae</i> found a target faster than the other two species in the sand column test. <b>Conclusion:</b> Isolates of <i>S. carpocapsae</i> have higher potential in targeting and pathogenicity of honeycomb moth larvae than the other two nematode species.</span></span></span></span></span></div> <span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"> <b><span dir="RTL" lang="FA" style="font-size:14.0pt"><span style="font-family:"B Nazanin""></span></span></b></span></span></span><br> <span style="font-size:11pt"><span style="line-height:normal"><span style="font-family:Calibri,sans-serif"><b><span style="font-size:12.0pt"><span style="font-family:"Times New Roman",serif"></span></span></b></span></span></span> شب­ پره موم­ خوار, Heterorhabditis, Steinernema Honeycomb moth, Heterorhabditis, Steinernema 89 99 http://yujs.yu.ac.ir/pps/browse.php?a_code=A-10-254-2&slc_lang=fa&sid=1 elmira Abootorabi المیرا ابوترابی elabootorabi@gmail.com 10031947532846004528 10031947532846004528 Yes Department of Nematology, Iranian Research Institute of Plant Protection, Tehran, Iran. بخش تحقیقات نماتدشناسی، موسسه تحقیقات گیاه پزشکی کشور، تهران Laleh Ebrahimi لاله ابراهیمی Ebrahimi.laleh@gmail.com 10031947532846004529 10031947532846004529 No Ardabil Agriculture and Natural Resources Research and Education Center, Ardabil, Iran مرکز تحقیقات، آموزش کشاورزی و منابع طبیعی استان اردبیل، اردبیل