Plant Pathology Science
دانش بیماری شناسی گیاهی
Plant Pathol. Sci.
Agriculture
http://yujs.yu.ac.ir/pps
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fa
jalali
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2021
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رابطه بین مقاومت سیبزمینی به پوسیدگی نرم باکتریایی و بیان سه ژن PR
The relationship between potato resistance to bacterial soft rot and expression of three PR genes
تخصصي
Special
پژوهشي
Research
<div><span style="font-family:B Nazanin;"><span style="font-size:14.0pt;">پاسالاری ح (1399) رابطه بین مقاومت سیبزمینی به پوسیدگی نرم باکتریایی و بیان سه ژن </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">PR</span></span></span><span style="font-family:B Nazanin;"><span style="font-size:14.0pt;">. دانش بیماریشناسی گیاهی 10(1): 85-76. </span></span></div>
<div dir="ltr"><span style="font-family:B Nazanin;"><span style="font-size:14.0pt;"> </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">Doi: 10.2982/PPS.10.1</span></span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">.76.</span></span></span><span style="font-family:B Nazanin;"><span style="font-size:14.0pt;">.</span></span></div>
<div style="text-align: justify;"><strong><span style="font-family:B Nazanin;"><span style="font-size:14.0pt;">مقدمه:</span></span></strong><span style="font-family:B Nazanin;"><span style="font-size:14.0pt;"> تغییر در مقاومت سیبزمینی به پوسیدگی نرم باکتریایی ممکن است با بیان ژنهای مرتبط با بیماریزایی</span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">(PR)</span></span></span><span style="font-family:B Nazanin;"><span style="font-size:14.0pt;"> ارتباط داشته باشد. هدف از اجرای این پژوهش بررسی رابطه بین سطح بیان سه ژن </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">PR</span></span></span><span style="font-family:B Nazanin;"><span style="font-size:14.0pt;"> و القای مقاومت در نتیجه آلودهسازی سلولهای بافت غدهای سیبزمینی با باکتریهای بیمارگر در دماهای مختلف به منظور مهار مؤثر بیماری پوسیدگی نرم باکتریایی سیبزمینی بود. <strong>مواد و روشها:</strong> </span></span><span style="font-family:B Nazanin;"><span style="font-size:14.0pt;">باکتریهای </span></span><em><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">Pectobacterium</span></span></span></em> <em><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">carotovorum </span></span></span></em><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">2</span></span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">A</span></span></span> <span style="font-family:B Nazanin;"><span style="font-size:14.0pt;">، </span></span><em><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">Pectobacterium</span></span></span></em> <em><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">atrosepticum</span></span></span></em><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">36</span></span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">A</span></span></span> <span style="font-family:B Nazanin;"><span style="font-size:14.0pt;">و </span></span><em><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">Dickeya</span></span></span></em><em><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">-</span></span></span></em><em><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">dadantii</span></span></span></em><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;"> ENA</span></span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">49</span></span></span> <span style="font-family:B Nazanin;"><span style="font-size:14.0pt;">انتخاب شدند. </span></span><span style="font-family:B Nazanin;"><span style="font-size:14.0pt;">رقم­های سیب زمینی </span></span><span style="font-family:B Nazanin;"><span style="font-size:14.0pt;">اسکارب نیمهمقاوم به بیماری </span></span><span style="font-family:B Nazanin;"><span style="font-size:14.0pt;">و ویسنیانکا </span></span><span style="font-family:B Nazanin;"><span style="font-size:14.0pt;"> حساس به بیماری، نیز</span></span> <span style="font-family:B Nazanin;"><span style="font-size:14.0pt;">برای آلودگی با باکتریها انتخاب شد</span></span><span style="font-family:B Nazanin;"><span style="font-size:14.0pt;">ند</span></span><span style="font-family:B Nazanin;"><span style="font-size:14.0pt;">. آزمایش بهصورت فاکتوریل بر پایه طرح کاملا تصادفی با سه تکرار انجام شد. تعداد نسخههای بیان شده </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">mRNA</span></span></span><span style="font-family:B Nazanin;"><span style="font-size:14.0pt;"> از </span></span><span style="font-family:B Nazanin;"><span style="font-size:14.0pt;">ژنهای </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">PR</span></span></span> <span style="font-family:B Nazanin;"><span style="font-size:14.0pt;">از طریق </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">PCR</span></span></span><span style="font-family:B Nazanin;"><span style="font-size:14.0pt;"> در زمان واقعی با کمک آغازگرهای مربوط به این ژنها </span></span><span style="font-family:B Nazanin;"><span style="font-size:14.0pt;">تعیین شد. </span></span><span style="font-family:B Nazanin;"><span style="font-size:14.0pt;">مقایسه میانگینها با آزمون </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">LSD</span></span></span> <span style="font-family:B Nazanin;"><span style="font-size:14.0pt;">انجام شد. <strong>یافتهها:</strong> میزان بیان ژنهای </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">PR</span></span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">-3</span></span></span><span style="font-family:B Nazanin;"><span style="font-size:14.0pt;">، </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">PR</span></span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">-5</span></span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">t</span></span></span><span style="font-family:B Nazanin;"><span style="font-size:14.0pt;"> و </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">PR</span></span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">-10</span></span></span><span style="font-family:B Nazanin;"><span style="font-size:14.0pt;"> در سلولهای غدههای سیبزمینی در پاسخ به آلودگی به </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">2</span></span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">A</span></span></span> <em><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">P</span></span></span></em><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">.</span></span></span> <em><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">carotovorum</span></span></span></em><span style="font-family:B Nazanin;"><span style="font-size:14.0pt;">، </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">36</span></span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">A</span></span></span> <em><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">P</span></span></span></em><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">.</span></span></span> <em><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">atrosepticum</span></span></span></em><span style="font-family:B Nazanin;"><span style="font-size:14.0pt;"> و</span></span><span style="font-family:B Nazanin;"><span style="font-size:14.0pt;"> </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">ENA</span></span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">49</span></span></span> <em><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">D</span></span></span></em><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">.</span></span></span> <em><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;">dadantii</span></span></span></em><span style="font-family:B Nazanin;"><span style="font-size:14.0pt;">، به دما و رقم سیب زمینی بستگی داشت. <strong>نتیجهگیری:</strong> تغییرات معنیدار در مقاومت سیبزمینی به بیماری پوسیدگی نرم باکتریایی در دماهای 28 و 33 درجه سلسیوس با سطح بیان این سه ژن </span></span><span dir="LTR"><span style="font-family:Times New Roman,serif;"><span style="font-size:12.0pt;"> PR</span></span></span><span style="font-family:B Nazanin;"><span style="font-size:14.0pt;">وجود دارد.</span></span></div>
Pasalari H (2021) The relationship between potato resistance to bacterial soft rot and expression of three PR genes. Plant Pathology Science 10(1):76-85. Doi: 10.2982/PPS.10.1.76.<span dir="RTL"></span><br>
<div style="text-align: justify;"><strong>Introduction:</strong> Changes in the resistance to bacterial soft rot in potatoes can be linked to the expression of pathogenesis-related (PR) genes. The aim of this study was to investigate the relationship between the accumulation of PR genes and the induction of resistance through infection of potato tuber cells with pathogenic bacteria at different temperatures in order to effectively combat bacterial soft rot disease in potatoes. <strong>Materials and Methods:</strong> <em>Pectobacterium carotovorum</em> 2A, <em>Pectobacterium atrosepticum</em> 36A, and <em>Dickeya dadantii</em> ENA49 were used in this study. For bacterial infection, the potato cultivars semi-resistant cultivar Scarab and susceptible cultivar Vesnianka, were used. The factorial experiment with three replications was carried out according to a completely randomized design. The relative level of mRNA copies of PR genes was determined by RT-PCR using primers of these genes<em>.</em> The mean values were compared according to the LSD test. <strong>Results: </strong>The experiments demonstrated the induction of PR-3, PR-5t and PR-10 in potato tuber cells in response to infection with <em>P. carotovorum</em> 2A, <em>P. atrosepticum</em> 36A and <em>D. dadantii</em> ENA49. It has been shown that the degree of induction of resistance genes depends on the temperature and the potato cultivar.<strong> Conclusion:</strong> It can be concluded that significant changes in potato resistance to bacterial soft rot at temperatures of 28 and 33 ° C are associated with the expression of these PR genes.<strong><em><span dir="RTL"></span></em></strong></div>
Dickeya, Pectobacterium, Scarab, Vesnianka
Dickeya, Pectobacterium, Scarab, Vesnianka
76
85
http://yujs.yu.ac.ir/pps/browse.php?a_code=A-10-336-2&slc_lang=fa&sid=1
hossein
pasalari
حسین
پاسالاری
hpasalary@yahoo.com
10031947532846003609
10031947532846003609
Yes
Department of Agriculture, Minab Higher Education Center, University of Hormozgan, Bandar Abbas, Iran
گروه کشاورزی، مجتمع آموزش عالی میناب، دانشگاه هرمزگان، بندرعباس، ایران