In order to investigate the effects of boron, zinc and sulfur on quantitative and qualitative characteristics of rapeseed (Hyola 401 cultivar), an experiment was conducted at Rice Research Institute of Iran, Rasht province in 2011. A field experiment with completely randomized block design was performed with eight treatments in three replications. Treatments consisted of control, zinc was added as Zn 15% EDTA at the rate of 1.5 kg ha^−1 were applied to the soil, Boron was added as Borax at the rate of 1.5 kg ha^−1, sulfur treatment added at the rate 100 kg ha^-1 before of sowing, B+Zn, B+S, Zn+S and B+Zn+S. Maximum grain yield (4157.6 kg ha^-1) was obtained from S+B+Zn treatments that increased the seed yield by 48.7% compared to the control. Maximum and minimum oil content obtained from B+Zn+S (42.58%) and control (38.37%). Maximum protein (24.62%) was obtained from zinc fertilizer. The highest leaf boron, zinc and sulfur content were obtained in treatments B+Zn+S of 15.7, 26.38 and 577.4 mg kg^-1 respectively, and minimum nutrients content was obtained in control. Regards to the experiment results, the application of B+S fertilizer increased the seed yield and oil content and is suggested in Rasht reign conditions.