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Showing 2 results for Spermidine

Leila Aslani, Mostafa Mobli, Mohammad Solemani,
Volume 2, Issue 1 (9-2015)
Abstract

The effect of spermidine on seed germination of three different cucumber cultivars under cold stress was studied as a 4×2 factorial experiment by using a completely randomized design with 4 replications. The first factor was 4 concentrations of spermidine consisted of 0 (control), 0.1, 0.5 and 1 mM and the second one was three cucumber cultivars consisted of ‘Emperator’, ‘Amiran’ and ‘Rashid’. For this purpose, 50 seeds of each cultivar were placed on filter papers inside sterilized Petry dishes and spermidine solutions were added to them according to each treatment. All petry dishes were placed at a 13°C temperature of the incubator until end of the experiment and germinated seeds were counted daily. The measured traits were the percentage of germination, plumule length, radicle length, the dry and fresh weight of the plumule and radicle. The results showed that treating seeds of ‘Rashid’ cultivar with 0.1 mM spermidine increased the percentage of germination, plumule and radicle length, fresh and dry weights of radical compared with control. Treating ‘Rashid’ seeds with high concentration (1 mM) of spermidine showed inhibitory effects on all measured characteristics, but in ‘Amiran’ cultivar only decreased the percentage of germination, plumule and radicle length, the fresh and dry weight of radicle.


Gilla Nazari, Mohammad Sedghi, Raouf Seyed Sharifi,
Volume 11, Issue 1 (9-2024)
Abstract

Extended abstract
Introduction: Germination and seedling growth are one of the most important stages of plant growth, which determine the degree of success of agricultural systems in production. Seed deterioration due to storage conditions is a challenge that results in diminished germinability poor seedling establishment, the loss of seed vigor, and finally seed mortality. Thus, it is necessary to use methods to increase seed germinability and enhance seedling establishment. The use of plant hormones is one of the methods that can increase seed germinability and optimal plant growth under seed deterioration conditions. Seed priming with plant hormones is among simple and cheap methods to improve seed germination, accelerate seedling growth and establishment, germination uniformity, and production of vigorous seedlings. The purpose of this experiment is to determine the most effective pretreatments of growth hormones to improve the germination and seedling establishment characteristics of deteriorated triticale seeds.
Materials and Methods: To examine the effect of priming on germination indices and some biochemical traits of deteriorated triticale seeds, a factorial experiment was performed in a completely randomized design with three replications at the Laboratory of Seed Science and Technology, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili in 2023. Treatments included seed deterioration (5%, 15%, and 25%) and priming (no prime, distilled water, cytokinin, spermidine, salicylic acid, and auxin).
Results: The results showed that seed deterioration reduced the germination percentage. Priming reduced the effect of seed deterioration and improved the germination percentage by 36.9%. As seed deterioration increased, radicle length, seedling length, vigor index, protein content, and protease activity decreased, whereas priming mitigated the effect of seed deterioration on these traits. At the lowest level of seed deterioration (25%), priming with auxin increased radicle length, plumule length, protease activity, and seedling length vigor index by 72.07, 62.06, 73.27, and 77.06, respectively, compared with no priming.
Conclusions: In general, it seems that triticale seed priming with auxin can invigorate deteriorated seeds and increase germination and seedling production uniformity.

Highlights:
  1. Auxin pretreatment had the greatest effect on improving the quality and germination characteristics of deteriorated triticale seeds.
  2. Effects of using growth regulators pretreatment were investigated in deteriorated triticale seeds.


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