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Showing 7 results for Hydropriming
Effects of Seed Priming Methods on Germination Parameters of Ajowan (Carum copticum) Seed
Somayeh Malekzade, Seyfollah Fallah2,
Volume 1, Issue 2 (1-2015)
Abstract
In order to investigate the effect of priming techniques on germination parameters of ajowan (Carum copticum L.) seed, an experiment was conducted in completely randomized design with four replications. The experiment treatments included a control (no priming), three hydropriming levels with distilled water (24, 36, and 48 h), three osmopriming levels with PEG (solutions with osmotic potential of -4, -8 and -12 bar), three hormone priming levels with GA3 (50, 100 and 150 ppm), three halopriming levels with KNO3 solution (2, 3, and 4%) and three zinc sulfate levels (0.1, 0.2 and 0.3 %). The results showed that priming treatments had a significant effect on all traits. The rate and percentage of germination were declined in some priming treatments compared to control. Radicle length and root dry weight were decreased in polyethylene glycol -8 bar. Polyethylene glycol -4 bar and 24h hydropriming treatments significantly decreased shoot length and allometry coefficient. However, the treatment of 100 ppm hormone priming significantly increased these traits. Hydropriming 24 h treatment significantly increased germination rate, percentage and seed vigor, but hydropriming 48 h significantly increased radicle and shoot length compared to control. In conclusion, according to the priority of germination or radicle, shoot growth and seed priming cost as well, the hydropriming 24 or 48 h can be recommended as most appropriate priming type for ajowan seeds.

Effect of Seed Priming on Some Germination Traits and Seedling Growth of Safflower under Drought Stress
Mozhgan Maleki Narg Mousa, Hamidreza Balouchi, Mahmood Attarzadeh,
Volume 2, Issue 1 (9-2015)
Abstract

In order to evaluate the effect of priming on some germination and seedling growth characteristics of safflower (Carthamus tinctorius L.) under drought stress, an experiment was conducted base on completely randomized design with four replications at the Seed Technology Laboratory of Yasouj University, in 2011. Treatments were included of five priming levels (control or no prime, hydro priming, potassium nitrate 3% (KNO3), PEG and Urea -4 bar) as the first factor and three levels of drought stress (0, -4 and -8 bar) as the second factor. The results showed that the interactions of drought stress and seed priming on germination percentage and the germination rate was not significant. But the main impact of priming and drought stress on them was so significant at 1% probability. The highest germination percentage and germination rate (75.66% and 3.62 seed.day-1, respectively) were shown in urea priming and the lowest (52% and 2.31 seed.day-1 respectively) in the control treatment (no prime). Drought stress was caused a significant decrease in germination traits. In general, the results obtained in this study showed that treatment with urea and potassium nitrate compared with PEG improved germination and seedling growth of safflower.


Effect of Hydropriming and Seed Size on Germination Characteristics and Seedling Growth of Vigna radiata Seeds under Salinity Stress
Ali Ghatei, Ghasem Parmoon, Zahra Amin Deldar, Hadi Ghamary,
Volume 2, Issue 2 (2-2016)
Abstract

In order to evaluate the effect of hydropriming on seed germination of Vigna radiata under salinity stress, an experiment was arranged in a factorial based on a completely randomized design with three replications in the laboratory of the Agricultural College of the University of Ramin Khuzestan. Treatments were salinity stress at 4 levels (0, 3, 6 and 9 Mmhos) and hydro priming at 3 levels (1, 1.5 and 3 h) and two sizes of seed (small and large seeds) that was carried out by NaCl. Results showed that all of the germination characteristics decreased with increased salinity, but this decrement for seeds that primed at a long time, was less. Also at all characteristics, small seeds, showed better reactions than large seeds. Generally, hydropriming was improved germination characteristics of Vigna radiata under saline conditions. According to this experiment, hydropriming improved germination and establishment.


The Effects of Different Priming Methods on the Storability and Germination under Salinity Stress in Rapeseed (Brassica napus) Line Karaj 3
Shirin Taghi Zoghi , Elias Soltani , Iraj Alahdadi , Reza Sadeghi ,
Volume 4, Issue 2 (3-2018)
Abstract

This study was conducted to study the effects of different priming methods on germination rate and percentage under salinity stress and to determine the stability of primed seeds. In order to accomplish this, three different experiments were conducted separately, including the experiment of water uptake, the experiment of salinity stress, and the experiment of storability of primed seeds. Priming treatments were five levels of control (unprimed), hydropriming (Hyd), priming with humic acid (HA), priming with salicylic acid (SA) and priming with gibberellic acid (GA). Salinity stresses were four levels of 0, 4, 8 and 12 ds/m of NaCl. The stability of prime seeds was investigated over a period of 226 days after priming. The results of water uptake showed that rapeseeds entered into the third phase of water uptake after 18 hours of hydration. The results of the salinity experiment showed that salinity levels of 12 and 0 ds/m had the lowest (74.3 %) and highest (83 %) germination percentage, respectively. In terms of germination rate, there were significant differences between GA (0.034 h-1), HA (0.036 h-1) and Hyd (0.036 h-1) with C (0.019 h-1) and SA (0.027 h-1). Generally speaking, primed seeds germinated better than control seeds at all levels of salinity. The storability of primed seeds and control seeds had no significant decrease during storage. Finally, it was concluded that seed priming increased the tolerance to salinity stress; in terms of storability, there was no significant difference between primed seeds and primed seeds could be stored in the same way as control seeds.

Highlights:

  1. At the current research, the stability of prime seeds was investigated for the first time.
  2. There was no significant difference between the storability of primed seeds and control (unprimed) at each sampling time (with an exception for SA).
  3. Primed seeds had better germination performance than control at the all salinity stress levels.
  4. Seed priming treatments using gibberellic acid, humic acid and hydropriming were the best compared with the other treatments.

The Effect of Priming on Seed Viability of Canola (Brassica napus) Cultivars under Different Storage Conditions
Mohsen Malek, Farshid Ghaderi-Far, Benjamin Torabi, Hamid Reza Sadeghipour,
Volume 6, Issue 2 (3-2020)
Abstract


Extended Abstract
Introduction: Priming is one of the most commonly used seed enhancement techniques. Events such as increased synthesis of nucleic acids, activation of repair processes, increased respiratory activity, and improved antioxidant capacity during priming lead to advanced metabolism in seeds. The most important effects of priming include increased percentage, speed and uniformity of germination and emergence. However, the longevity of primed seeds in storage is the major concern for researchers as it restricts widespread use of this technique. Some researchers believe that priming reduces the storage capacity of seeds, while others have reported increased seed shelf life after using priming treatments. Therefore, this study sought to investigate the effects of priming on the storage capacity of the seeds of canola cultivars under different storage conditions.
Material and Methods: In this study, the effects of priming on the shelf life of seeds of three canola cultivars including Dk-xpower, Traper and Hayola50 were investigated. For this purpose, the seeds were first treated with hydropriming and osmopriming methods. Then primed and control seeds with 6, 9, 12 and 15% moisture content were stored for 8 months at 15, 25, 35 and 45 °C. Sampling from different seed treatments was carried out at intervals of 1 to 30 days to assess germination. Finally, by fitting a three-parameter logistic model to cumulative germination data versus the day after storage, the time to germination loss to 50% was calculated and used to compare seed storage behavior between the treatments.
Results: The results showed that the storage behavior of canola seed varies greatly depending on the cultivar, and each cultivar showed a distinct behavior. Priming effects on the shelf life of seeds were different depending on the storage conditions, cultivars and also the priming methods. Comparison of the effects of priming on the seeds’ shelf life under different storage conditions showed that priming treatments were more efficient under higher seed moisture content and storage temperatures than those with lower seed moisture content and storage temperatures. In addition, priming treatments in Dk-xpower cultivar often increased the seeds’ shelf life. However, in the Traper and Hayola 50 cultivars, hydropriming often improved the seeds’ shelf life, and in contrast to osmopriming, it led to a decrease in the shelf life of the seeds.
Conclusion: Based on the results of this study, it was shown that priming effects on canola seed viability can be a function of various factors such as cultivar, storage conditions, and also the type of priming treatment. Moreover, in this study, hydropriming often increased seed longevity whereas osmopriming often increased the deterioration rate and reduced seed longevity.
 
 
Highlights:
  1. Seed storage behavior of canola cultivars was compared under natural storage conditions.
  2. Priming effects on seed longevity of canola cultivars was investigated under different storage conditions.

Evaluating the possibility of increasing freezing tolerance in chickpeas (Cicer arietinum) by seed priming
Mohammad Mohammadi, Reza Tavakol Afshari, Jafar Nabati, Ehsan Oskoueian,
Volume 9, Issue 2 (3-2023)
Abstract
Extended Abstract
Introduction: One of the major reasons behind the unstable yield of chickpea, is the simultaneity of the reproductive stage with drought and late-season heat. Autumn sowing of chickpea is among the suitable approaches to improve chickpea yield. On the other hand, freezing stress is a limiting factor in the autumn sowing of chickpea. Recently, seed priming has been developed as an essential method to induce plant tolerance to environmental stress. The priming will result in a rapid response of the plant to stress. Freezing, as an environmental stress, limits the growth and development of many plants in different parts of the world. Studies show that in addition to acclimation, short-term biotic and abiotic stresses as pretreatment could also increase the plant's tolerance to cold stress. This process alters the freezing response positively.
Material and Methods: This experiment was conducted as a factorial in a completely randomized design with three replicates at the greenhouse of the Research Center for Plant Sciences of Ferdowsi University, Mashhad Iran, in 2018. The experimental factors consisted of various temperatures (0, -12, -15, and -17 °C), seed priming at 10 levels (control (without priming), hydropriming, priming with sodium chloride, salicylic acid, sodium nitroprusside, phosphate solubilizing bacteria and potassium solubilizing bacteria, amino acids, potassium nitrate, and zinc sulfate) and different chickpea genotypes (MCC505, ILC8617, MCC495, and Saral cultivar). In this experiment, the measured parameters included survival percentage, electrolyte leakage percentage, and lethal temperature resulting in 50% mortality according to the electrolyte leakage and survival percentage.
Results: The results showed that the application of hydropriming, priming with sodium nitroprusside and zinc sulfate had favorable effects on the survival rate and electrolyte leakage. Among these, priming with sodium nitroprusside increased the survival percentage compared to the control (23%) at the -15 and -17 °C in the Saral cultivar, at -15 °C in the ILC8617 genotype, and at -12 and -15 °C in the MCC495 genotype treatment to 68, 58, 85 and 55 percent, respectively. In addition, this treatment reduced the electrolyte leakage by 13% at -15 °C in the ILC8617 genotype compared to the control treatment. Further, the mentioned treatment resulted in a 40% reduction in lethal temperature resulting in 50% mortality according to the survival percentage. In the MCC495 genotype compared to the control treatment.
Conclusion: Overall, the cold stress in the chickpea plants resulted in an increase in electrolyte leakage and a decrease in the survival percentage. Application of sodium nitroprusside priming by improving cold stress tolerance resulted in a reduction of lethal temperature resulting in 50% mortality based on electrolyte leakage and survival percentage results. Additionally, the applied priming in improving the cold stress tolerance mainly improved the survival percentage compared to the improvement in the electrolyte leakage.

Highlights:
  1. The effect of different primings on the freezing tolerance of chickpeas was investigated and determined.
  2. The freezing tolerance threshold of chickpea seedlings was determined at the laboratory under different primings.
  3. The respondents of genotypes to priming and the behavior of genotypes towards each other were investigated.

Effect of sodium nitroprusside priming on seed germination indices and seedling biochemical traits in rice (Oryza sativa) under salinity stress
Haniyeh Saadat, Mohammad Sedghi,
Volume 11, Issue 1 (9-2024)
Abstract
Extended abstract
Introduction: Salinity is the most significant environmental stress that limits plant productivity by affecting morphology, physiology, and biochemistry of plants, especially in semi-arid and arid regions. Salinity disrupts and eventually delays seedling growth by delaying seed germination and reducing the germination rate. Seed priming stands out as a quick, easy, low-cost, and effective strategy for improving germination, seedling growth parameters, and overall plant defense against abiotic stresses in many crops. It is defined as the pre-sowing seed treatment during which seeds are immersed in water or chemical solutions and are dry until further use. The aim of this study was to assess the effect of priming with sodium nitroprusside on germination indices and biochemical traits in rice seedlings under salinity stress.
Materials and Methods: This experiment was conducted as a factorial based on a completely randomized design with three replications at the University of Mohaghegh Ardabili in 2023. Experimental treatments included four salinity levels (0, 50, 100, and 150 mM) and three levels of sodium nitroprusside (0, 40, and 80 µM).
Results: The results showed that salinity reduced germination and growth indicators including mean daily germination (MDG), germination coefficient (GC), allometric coefficient (AC), radicle length (RL), pedicel length (PL) and seedling length (SL), as well as radicle and pedicel fresh and dry weight (RFW, PFW, RDW and PDW), but seed pretreatment with different levels of sodium nitroprusside, especially the level of 80 µM, improved these traits. Salinity reduced the seedling moisture percentage (SMP), so that the highest SMP (70.13%) was observed in the control treatment. The highest daily germination rate (DGR) and malondialdehyde content (MDA) were observed at a salinity of 150 mM. Priming decreased DGS and MDA, so that the lowest DGS (0.08) and MDA (0.159 mM g-1 FW) were obtained in priming with 80 μM sodium nitroprusside. Also, salinity decreased the activity of the α-amylase enzyme, so the lowest α-amylase activity (7.93 mg g-1 FW seed) was obtained in the control (distilled water) and at a salinity of 150 mM.
Conclusions: The results showed that seed treatment with sodium nitroprusside at 80 µM is the most effective method to improve rice germination and biochemical traits under salinity stress. It can reduce the harmful effects of salinity on some traits in rice seedlings and improve seedling growth.

Highlights:
  1. Seed priming using sodium nitroprusside improved the germination indices of rice seeds under salinity.
  2. Priming with sodium nitroprusside decreased MDA content and increased α-amylase activity.
  3. The concentration of 80 µM sodium nitroprusside showed a better effect on germination indices and biochemical characteristics.


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