Iranian Journal of Seed Research

Germination is the first and the most stages for sainfoin (Onobrychis viciifolia) growth; which it has especially sensitivity to environmental stresses such as alkalinity. The current study was conducted to evaluate the effectiveness of seed pre-treatment methods on improving sainfoin tolerance to alkali stress in germination, early growth and its recovery as a factorial experiment based on a completely randomized design in College of Agriculture, Shiraz University in 2013. The first factor was four seed pre-treatment methods (seed with the pod, seed without the pod, pre-chilling of seed without pod and hydro-priming of seed without pod) and the second factor was five alkali stress levels (pH= 6.7, 7.9, 8.9, 9.8 and 10.7). Alkali stress levels were prepared using two neutral salts (Na₂SO₄ and NaCl) and two alkaline salts (NaHCO₃ and Na₂CO₃). The results showed that germination percentage was decreased as alkalinity (pH) levels were enhanced; so that, there were no germinated seeds in 9.8 and 10.7 treatments. Alkali stress reduced initial germination percentage (51.9%), final germination percentage (51.8%), plumule length (55.7%) and radicle length (72.2%). Under all alkalinity conditions, the lowest seed germination and seedling growth were observed in with pod seed, followed by pre-chilling treatments; while their highest were achieved in without pod seeds and hydro-priming treatments. The highest recovery was observed in without the pod, followed by hydro-primed seeds. Recovery was observed in Pre-chilled and with pod speeds up to 7.9 and in hydro-primed and without pod speeds up to 8.9.

The present study sought to compare the effect of seed priming by hydropriming, ascorbate and calcium chloride (CaCl₂) versus different concentrations of silver nanoparticles on two Iranian rice cultivars (Hashemi and Shiroodi) under drought stress. The experimental design, which was factorial, was completely randomized. The first factor was control (without priming), hydro-priming, 20 mg.L^-1 calcium chloride, 20 mg.L^-1 ascorbates and 20, 40 and 80 mg.L^-1 of silver nanoparticles. The second factor was the drought stress, including control, -1.48 and -4.91 bars created by polyethylene glycol 6000 solutions. In plants without drought stress, the time required to reach 50% germination (T₅₀) reduced to 34 and 54 percent under ascorbate and CaCl₂ priming in the Hashemi and Shiroodi cultivars, respectively.  In the drought stress condition, ascorbate and hydropriming pretreatments decreased T₅₀ and the time required to reach 90% germination (T₉₀) more than silver nanoparticles, especially in the Shiroodi cultivar. On the other hand, in comparison with other pretreatments, CaCl₂ had an important role in increasing the growth factors in all of the drought stress treatments. Although 40 mg.L^-1 silver nanoparticles increased the growth factors to some extent, it seems that higher levels of silver nanoparticles cause stress in seeds and therefore decrease the seed germination and growth of seedlings. By decreasing reactive oxygen species, Ascorbate improves the germination and by increasing plasma membrane efficiency, CaCl₂ enhances the seedling growth under drought stress.

Highlights:

1. Silver nanoparticles increased the effect of stress on the seedlings of rice cultivars.
2. Ascorbate and calcium chloride improved the germination and seedlings growth of the rice cultivars, respectively under drought stress conditions.