Iranian Journal of Seed Research

The present study sought to compare the effect of seed priming by hydropriming, ascorbate and calcium chloride (CaCl₂) versus different concentrations of silver nanoparticles on two Iranian rice cultivars (Hashemi and Shiroodi) under drought stress. The experimental design, which was factorial, was completely randomized. The first factor was control (without priming), hydro-priming, 20 mg.L^-1 calcium chloride, 20 mg.L^-1 ascorbates and 20, 40 and 80 mg.L^-1 of silver nanoparticles. The second factor was the drought stress, including control, -1.48 and -4.91 bars created by polyethylene glycol 6000 solutions. In plants without drought stress, the time required to reach 50% germination (T₅₀) reduced to 34 and 54 percent under ascorbate and CaCl₂ priming in the Hashemi and Shiroodi cultivars, respectively.  In the drought stress condition, ascorbate and hydropriming pretreatments decreased T₅₀ and the time required to reach 90% germination (T₉₀) more than silver nanoparticles, especially in the Shiroodi cultivar. On the other hand, in comparison with other pretreatments, CaCl₂ had an important role in increasing the growth factors in all of the drought stress treatments. Although 40 mg.L^-1 silver nanoparticles increased the growth factors to some extent, it seems that higher levels of silver nanoparticles cause stress in seeds and therefore decrease the seed germination and growth of seedlings. By decreasing reactive oxygen species, Ascorbate improves the germination and by increasing plasma membrane efficiency, CaCl₂ enhances the seedling growth under drought stress.

Highlights:

1. Silver nanoparticles increased the effect of stress on the seedlings of rice cultivars.
2. Ascorbate and calcium chloride improved the germination and seedlings growth of the rice cultivars, respectively under drought stress conditions.

Objective: This study aimed to evaluate the effect of chitosan on germination indicators and the activity of antioxidant enzymes in safflower seedlings under salinity stress.
Method: The experiment was conducted using a factorial arrangement based on a completely randomized design with three replications at the University of Mohaghegh Ardabili in 2024. The experimental treatments included four salinity levels (0, 50, 100, and 150 mM NaCl) and four concentrations of chitosan (0, 0.2, 0.4, and 0.5% w/v), which were dissolved in 1% acetic acid.
Results: The results showed that salinity stress reduced the germination rate, radicle length, plumule length, seedling length, seedling fresh weight, and seedling dry weight. However, priming with different concentrations of chitosan, especially at 0.5%, improved these traits. The highest daily germination rate (0.114) was observed in the control group (distilled water priming) under 150 mM salinity. The activity of catalase and peroxidase enzymes in the control under 150 mM salinity increased by approximately 43% and 70%, respectively, compared to the 0.5% chitosan treatment under non-saline conditions. Similarly, the activity of superoxide dismutase enzyme in the 0.5% chitosan treatment under 150 mM salinity increased by about 67% compared to the control under non-saline conditions. Furthermore, the ascorbate peroxidase enzyme activity in seeds primed with 0.5% chitosan increased by 37% compared to the control (distilled water priming).
Conclusions: The results indicated that seed treatment with different concentrations of chitosan can mitigate the harmful effects of salinity on some traits of safflower seedlings and improve seedling growth. The best results were achieved when 0.5% chitosan was used under salinity conditions.

Highlights