Search published articles

Showing 9 results for Drought Stress

Mansour Taghvaei, Neda Aliolad,
Volume 1, Issue 2 (1-2015)

Trittipyrum is an important annual grass plant for forage production in arid and semiarid regions. The effects of drought stress on Trittipyrum lines were evaluated in the field experiment in a randomized complete block design with a factorial experiment involving three replications in 2012. Treatments were water stress (field capacity (FC), 75% of FC, 50% of FC and 25% of FC throughout the experimental period and 13 primary Trittipyrum lines ((St/b)(Cr/b)F4, (Ma/b)(Cr/b)F4, (Ma/b)(Cr/b)F3, (Ka/b)(Cr/b)F2, (Ka/b)(Cr/b)F3, (Ka/b)(Cr/b)F5, (Ka/b)(Cr/b)F6, La(4b,4d)/b, St/b, Cr/b, La/b, Ka/b, Az/b). The results showed that drought stress had significant effects on seedling traits. Drought stress significantly decreased shoot length, root length, shoot dry weight, root dry weight, seedling dry weight, seedling length vigor index and seedling weight vigor index. The results of mean comparison revealed that (az/b)(cr/b)F5 and Kz/b lines were the most sensitive and the most tolerant lines to drought stress in early vigor stage, respectively. Although the response of Trittipyrum lines was different to drought stress; but the result indicated that Trittipyrum lines are sensitive to drought at emergence stage.

Rahele Ahmadpour, Saeedreza Hosseinzadeh, Nezam Armand, Ebrahim Fani, Fariba Noedoust,
Volume 2, Issue 1 (9-2015)

Rapid germination is an important factor determining the final yield. This study was performed to investigate the effects of different levels of methanol and drought stress on germination characteristics of lentil seedlings. The experiment was conducted in completely randomized design with three replications in summer 2014 at the Khatam Alanbia University of Behbahan. The first factor was different levels of methanol equal to 0 (control), 5, 10 and 15 volumetric percentage (v/v), and the second factor we negative water potential in four levels 0, -3, -6 and -9 bar by PEG. Results showed that there was a significant difference between different methanol concentrations regarding germination percentage, germination speed index, plumule and radical length, plumule and radical dry weight, radical area, radical diameter and consumed endosperm (P 0.01). Different levels of methanol caused a significant decrease in germination characteristics compared with to control. Drought stress with -9 bar level significantly decreased germination percentage, germination speed index, plumule and radical length, plumule and radical dry weight, radical area, radical diameter and consumed endosperm compared to other levels. Effects of drought and methanol were significant differences regarding the germination speed index, plumule length, plumule dry weight and consumed endosperm (P 0.05).

Seyedeh Arhameh Fallah-Shamsi, Hemmatollah Pirdashti, Aliakbar Ebadi, Masoud Esfahani, Mahmood Raeini,
Volume 4, Issue 1 (9-2017)

In order to study drought stress effect at the reproductive stage on seed germination characteristics of seven rice genotypes, an experiment was carried out at Sari Agricultural Sciences and Natural Resources University in 2014. This study was conducted on drought-stressed seeds and seeds grown under normal conditions. The results of orthogonal mean comparison among non-stressed seeds showed that local genotypes had a lower germination index, germination rate, water percentage of seedling tissue, shoot length and root dry weight than the improved genotypes. Among stressed seeds, however, local genotypes had a higher germination index and germination rates than those of improved genotypes. Comparison of non-stressed seeds of susceptible and tolerant genotypes indicated that susceptible genotypes had a higher germination index, germination rate and germination percentage than tolerant genotypes. On the other hand, the comparison of the stressed seeds of susceptible and tolerant genotypes revealed that drought stress increased the time to 10, 50, 90 and 95% germination, water percentage of seedling tissue and plantlet length in susceptible genotypes, which were higher than those of tolerant genotypes. In conclusion, it seems that drought stress at reproductive stage has a significant effect on germination characteristics of the plant’s following generation. However, their responses are different, depending on the level tolerance of the genotype to stress and genetic origin.

1-  Drought stress at reproductive stage has a significant effect on germination characteristics of the following generation of the rice plant.
2-  Among stressed seeds, local genotypes have a higher germination index and germination rates than those of the improved genotypes.
3-  Drought stress increases the time of germination in susceptible genotypes, which is higher than that in the tolerant genotypes.

Hasan Teimori, Hamidreza Balouchi, Ali Moradi, Elias Soltani,
Volume 5, Issue 2 (3-2019)

Extended abstract
Introduction: Seed germination is one of the first important and complex stages in the plant life cycle and is affected by many hereditary and environmental factors. Various factors affect germination and seedling establishment. Among these factors are the characteristics of the maternal plant (nutrition, genetics), seed treatment stage at harvest time, as well as environmental factors (temperature, water potential, and ventilation and soil compaction). Also, under the influence of seed loss during storage, seed vigor, which is known as the first component of seed quality, decrease. The aim of this study was investigation of germination and biochemichal responses of the aged seed of Fenugreek to different temperature and humidity ranges.
Materials and Methods: This experiment was conducted as a factorial based on a completely randomized design with four replications in the Laboratory of Seed Science and Technology, Faculty of Agriculture, Yasouj University in 2016. The experimental treatments consisted of nine levels of temperature (5, 10, 15, 20, 25, 30, 35, 40 and 45 degrees Celsius), water potential included seven levels (zero (control), -0.2, -0.4, -0.6, -0.8, -1 and -1.2 MPa) and seed aging at two levels (no aged (control) and aged seed).
Results: In this experiment, the effect of seed aging, water potential and their interactions on each environment on germination indices (germination percentage and germination rate, length and weight vigor index) and biochemical indices (soluble sugar, proline, soluble protein and catalase enzymes) of Fenugreek seeds were significant. The results showed that in the aged seed the germination percentage and rate and seedling vigor index tended to decrease with water potential reduce in temperature lower and higher than 20 degrees Celsius, and the amount of biochemical components of the seed (soluble sugar, soluble protein, proline, and catalase enzyme) also increase.
Conclusion: In general, germination and biochemical indices of seed of Fenugreek are sensitive to water potentials, aging, and seed germination temperatures, respectively. In terms of osmotic potential decrese, the germination temperature of less than 20 ° C resulted in increased germination resistance of fenugreek seed to a more negative water potential.
  1. Study of germination and biochemical properties of fenugreek seed aged under different level of osmotic potantials and temperatures.
  2. In areas with a lower osmotic potential it is better to cultivate Fenugreek seed at temperatures below 20 °C.

Asgar Ganje, Ali Ebadie, Ghasem Parmoon, Soodabeh Jahanbaksh,
Volume 7, Issue 2 (3-2021)

Extended Abstract
Introduction: Water deficit stress is one of the important factors affecting seed germination. This stress decrease germination rate and affects germination percentage under high levels as well. Seed priming improves germination by changing metabolic activities before radicle emergence. This investigation was conducted to evaluate the effect of seed priming by salicylic acid on the emergence index and grain weight of spring wheat under water deficit stress.
 Material and Method: These experiments were conducted as factorial based on the completely randomized design with three replicates in the greenhouse of the Faculty of the Agriculture Sciences University of Mohaghegh Ardabili. Experiment treatments included different salicylic acid concentrations (0, 1, 2 mM) and water deficit stresses (25%, 45%, 65% and 85% Field capacity).
Result: According to results, emergence index (emergence percentage and emergence rate, uniformity emergence, and times to 10, 50, and 90% emergence) was influenced at 1% by water deficit stress. The effect of salicylic acid was significant on the emergence index. The interactions of water deficit stress and salicylic acid were effective at 1% on emergence uniformity and time to 90% emergence. Changes in plant height and heading rate were lower under water deficit stress. Also, both slope (b) and maximum content (a) decreased by water stress (22% and 7%). Priming by salicylic acid at 2 mM had the highest effect on both traits and resulted in a decrease in their slopes (17% and 13%) and an increase in their maximum content (34 and 10%). Plant dry weight was influenced by the interactions of water deficit stress and salicylic acid at 5% level. The plant's final height and grain weight was influenced by water deficit stress and salicylic acid. The highest grain weight was obtained at 80% and 60% field capacity with means of 0.79 and 0.75 g, which had no significant differences with eachother. The heights grain weight (0.72 g) was obtained by application of  2 mM salicylic acid which led to an increase of 250% compared to control.
Conclusions: Overall, we showed that water deficit stress resulted in decreased emergence rate and emergence percentage of wheat and finally declined plant growth and grain weight. Application of salicylic acid in seed priming resulted in improvement in the emergence index, growth, and grain weight of wheat. Also, the highest grain weight was observed at 2 mM concentration and it can be considered as the enhancing treatment.

1- Response of plant growth stage to stress was quantified using nonlinear regression
2- Relationship between emergence and grain weight was investigated under stress and priming by salicylic acid.

Mahnaz Mansouri, Ali Moradi, Hamidreza Balouchi, Elias Soltani,
Volume 8, Issue 1 (9-2021)

Extended abstract
Introduction: Seed is the most important sexual reproductive factor in plants that plays an important role in transmitting hereditary characteristics, plant distribution, and establishment in different regions. Seed germination as one of the most important and complex stages in the life cycle of plants is affected by genetic and environmental factors. Drought stress is one of the main barriers of crop plants and trees, production in many parts of the world, especially in arid and semi-arid regions such as Iran. The purpose of this study was to investigate the effect of drought stress on biochemical and seed germination indices of different seed lot of Ziziphus spina–christi as suitable medicinal species for controlling soil erosion in arid regions.
Materials and Methods: This experiment was conducted as a factorial based on a completely randomized design with four replications at the Seed Science and Technology Laboratory of Yasouj University in 2018. Experimental factors included Ziziphus spina–Christi seeds collected from the regions of Minab, Kazeroon, Masjed Soleiman, and Dehdasht and five water potentials including zero, -2, -4, -6, and -8 bar.
Results: In the present study, the effect of osmotic potential, seed lot, and their interaction were significant in each seed lot on germination (germination percentage, germination rate, seedling length, allometric index, longitudinal vigor) and biochemical (hydrogen peroxide, soluble protein content, Proline, catalase, and ascorbate peroxidase) indices of Ziziphus spina–christi. The results showed that except for allometric index response of all studied germination traits to osmotic potential was decreasing, Also with increasing osmotic potential, seed soluble protein was decreased and the amount of proline and activity of catalase and ascorbate peroxidase increased. With increasing osmotic stress, the root length was significantly increased, which increased the allometric coefficient of the seedling.
Conclusion: Germination and biochemical indices of seeds of all seedlots had significant changes with the reduction of osmotic potential and the reduction of osmotic potential was associated with reduced germination and plant establishment capability. These results can help us to recognize the prerequisites of germination and the development of the forests.

1- Germination indices of seeds related to several Ziziphus spinachristi seed lots were investigated under different water potentials.
2- Biochemical aspects related to seed dormancy in several Ziziphus spinachristi seed lots were evaluated.

Seyedeh Tina Mousavi Kani, Davoud Kartoolinejad, Zohreh Bahrami, Ali Asghar Zolfaghari, Elahe Nikouee,
Volume 9, Issue 1 (9-2022)

Extended Abstract
Introduction: Nowadays, due to land use change and destruction of natural resources induced by human activities, attention to desertification has immensely become widespread. One of the most important species for biological rehabilitation of desert areas on a large scale is the hawthorn plant. Hawthorn is a plant belonging to the family Amaranthaceae, the two species of which are called white saxaul (Haloxylon persicum Bunge ex Boissier & Buhse) and black saxaul (Haloxylon aphyllum (Minkw.) Iljin) are more notorious and widespread in Iran. These plants own extensive root systems and stabilize running sands. The aim of this study was to investigate the effect of mesoporous titanium dioxide nanoparticles on germination traits of black saxaul seeds under drought stress, as an important species in desert areas.
Materials and Methods: The experiment was performed as a factorial experiment in a completely randomized design with 3 replications. Experimental treatments included five titanium nano dioxides with concentrations of 0, 25, 50, 100 and 200 mg / l and six drought stress levels including 0, -4, -8, -12, -16 and -20 bar. After nanopriming, black saxaul seeds were subjected to drought stress for 15 days in a germinator at a temperature of 20 °C with four replications. After germination, in each of the treatments, germination percentage (GP), germination rate (GS), mean germination time (MGT) and seed vigor index (VI) were calculated.
Results: The results of two-way ANOVA and mean comparison of germination indices showed that the highest germination percentage (74%) belonged to the zero level of stress treatment with a concentration of 200 mg/l and the lowest one (3%) belonged to -20 bar treatment with 25 and 200 mg/l nano-titanium dioxide. With increasing drought stress intensity from zero to -20 bar level, germination rate decreased from 7.01 to 0.43 seeds per day. The highest amount of seed vigor index at zero drought level belonged to the treatment of 200 mg/l concentration of nano-titanium, with a rate of 325.77.
Conclusion: The results showed that germination indices and initial growth of black saxaul seeds decreased in all primed and unprimed treatments with increasing drought stress, and nanopriming with titanium dioxide had a positive effect on seed germination traits.


1- Seed priming using mesoporous nano-titanium dioxide improved the germination percentage of black saxaul seeds under drought stress.
2- The concentration of 200 mg/l of mesoporous nano titanium dioxide revealed a better effect on seed germination index, mean germination time, germination rate and percentage
3- Seed nano priming using titanium dioxide did not have much effect on the growth characteristics of black saxaul seedlings at higher levels of drought stress.
Hassan Gholami, Roohollah Abdolshahi, Mehdi Mohayeji, Mohsen Esmaeilizadeh-Moghadam,
Volume 9, Issue 2 (3-2023)

Extended Abstract
Introduction: Wheat coleoptile protects the plumule and the first leaf so they can move from the embryo to the soil surface. Coleoptile is essential for plant establishment. Cultivars with longer coleoptiles and mesocotyls are sown deeper and are more successful under drought stress conditions. However, there is not much information about their genetics. The objective of the present study was to evaluate the coleoptile and mesocotyl of Iranian, overseas and the lines developed as a part of Shahid Bahonar University breeding program, and estimate of genetic parameters of these traits.
 Materials and Methods: In this research, 30 bread wheat genotypes originated from Iran, CIMMYT, ICARDA, USA, and Australia and five lines from Shahid Bahonar University of Kerman breeding programs were sown at a 10 cm depth of soil in the research field of Shahid Bahonar University in a randomized complete block design with seven replications. Each plot consisted of three rows with two meters long and a 5 cm intra-row spacing. At harvest, the plants were gently removed from the soil, and after removing the soil from the root area, the roots were washed. In this study, coleoptile and mesocotyl length, number of seminal roots, root length, root and shoot dry weight were measured.
Results: Coleoptile and mesocotyl are important traits for increasing drought tolerance in bread wheat. In the present research, mesocotyl length varied from 5.20 for Excalibur to 2.08 for Zagros, and showed a high heritability (0.48) and response to selection (11.61%). Furthermore, this trait had a positive significant correlation with coleoptile length (r=0.53**), root weight (r=0.38*) and shoot weight (r=0.36*). Seminal and nodal root number had the highest (0.59), while root length had the lowest (0.13) narrow-sense heritability. Overall, suitable cultivars for rain-fed conditions had higher coleoptile and mesocotyl than the others. Breeding lines in Shahid Bahonar University of Kerman suitable for rain-fed conditions had longer coleoptile and mesocotyl lengths. Root and shoot dry weight had a significant positive correlation (r=0.82**).
Conclusions: In general, breeding programs to increase grain yield differ under rain-fed and irrigated conditions. In wheat breeding programs under rain-fed conditions, special attention should be paid to coleoptile and mesocotyl traits. These traits were not influenced by the plant growth habit.

  1. Mesocotyl of the Iranian cultivars was evaluated for the first time.
  2. Breeding lines developed by Shahid Bahonar University of Kerman suitable for rain-fed had appropriate coleoptiles and mesocotyls.
  3. Coleoptile and mesocotyl had a significant and positive correlation.

Arezoo Paravar, Saeideh Maleki Farahani, Alireza Rezazadeh,
Volume 9, Issue 2 (3-2023)

Extended abstract
Introduction: Lallemnatia ibercia (Dragon’s head) is an annual herb, which belongs to the Lamiaceae family. Nowadays, its seed is widely used in food and pharmaceutical industries due to its high mucilage and oil content. Soil moisture, nutrition, light, and temperature of the maternal environment play an important role in plant growth. However, the effect of soil moisture on yield and seed quality indices is greater during seed development. The positive effects of maternal plant nutrition with mycorrhizal inoculation can influence the quality improvement of the seeds developed under water deficit conditions. The reason for the quality improvement of the seeds developed under mycorrhizal inoculation condition is reported to be their water balance under water deficit conditions and as a result, more absorption of water and nutrients. Nutrient uptake by the maternal plant can play an important role in improving the germination and quality characteristics of the seeds developed under drought stress.
Materials and Methods: A field experiment was conducted in a completely randomized design with three replications at the Seed Science and Technology Laboratory of the College of Agriculture, Shahed University during f 2018-19 and 2019-20 years. Experimental treatments included irrigation regime (30, 60 and 90% of depletion of available soil water (ASW)) and mycorrhizal inoculation (non-inoculation and inoculation).
Results: The results showed that a reduction in soil moisture in the maternal environment decreased germination percentage and seed quality.  Germination percentage (51.20%) germination rate (7%), seed vigor index (27.70%), seedling length (28.3%), seedling dry weight (34.80%), seed mucilage (75.40%), as well as seed nutrients (27.30%), seed oil (14.60%) and catalase (35.25%) and ascorbate peroxidase (25.70%) enzymes activities enhanced in the seeds developed under 60% depletion of available soil water and application of mycorrhizal compared to control (30% depletion of available soil water and without mycorrhizal). Compared with the 30% depletion of available soil water and without mycorrhizal, mean germination time (17%) and lipid peroxidation (41.10%) increased under the 90% depletion of available soil water and without fertilizer mycorrhizal.
Conclusions: According to the results of this study, the irrigation regime of 60% available soil water depletion of maternal plants leads to the reduction of seed quality. However, the nutrition of maternal plants by mycorrhizal inoculation effectively improved the quality of seeds developed under water deficit due to the absorption of water and nutrients.

  1. Germination and seedling growth indices, quality and biochemical characteristics of the Lallemantia iberica seeds developed under different conditions of irrigation regime were investigated.
  2.  The effect of mycorrhiza on nutrient uptake, oil content, and antioxidant enzyme activity of the seeds developed under different irrigation regimes was investigated.
  3. The extent of damage to the lipid structures of cells in the seeds developed under different irrigation regimes and mycorrhiza fertilizer was investigated.

Page 1 from 1     

© 2023 CC BY-NC 4.0 | Iranian Journal of Seed Research

Designed & Developed by : Yektaweb

This work is licensed under a Creative Commons Attribution 4.0 International License.