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Showing 30 results for Seedling
Hamdollah Eskandari, Ashraf Alizadeh-Amraie,
Volume 1, Issue 1 (5-2014)
Volume 1, Issue 1 (5-2014)
Abstract
A laboratory experiment was conducted to evaluate the effect of seed priming under salt and drought conditions on seed germination and early seedling development of millet. A factorial experiment (3×2×5) based on completely randomized design with three replications was employed. The first factor was the effect of seed priming (control, hydro priming and KNO3), the second factor was the effect of salt and drought stresses including NaCl and PEG 6000 and the third factor was the effect of osmotic potential levels (-0.3, -0.6, -0.9 and -1.2 MPa). Results showed that germination performance was negatively affected by decreasing osmotic potential. There was a variable germination with different stress condition, in which seeds were able to germinate at all concentration of NaCl but no significant germination was occurred at -0.6 MPa of PEG for no primed and KNO3. However, both seed priming treatments (Hydropriming and KNO3), improved seed germination performance with the clear effectiveness of Hydropriming in improving germination properties under salt and drought conditions. It was concluded that germination inhibition resulted from osmotic effect rather than salt toxicity.
Ghorbanali Rassam, Samaneh Rahban, Mahnaz Mojtabaii, Atefeh Badri,
Volume 1, Issue 2 (1-2015)
Volume 1, Issue 2 (1-2015)
Abstract
Seed quality is one of the most important factors affecting seed germination and seedling growth. In order to investigate the effects of seed aging on germination and seedling growth of sunflower cultivars, a factorial experiment was conducted based on a completely randomized design with three replications in seed research laboratory of Shirvan Higher Education Complex. To create 7 different treatments of seed aging, seeds of three cultivars Ghasem, Farrokh and Barzgar (as the first factor) were kept at a high temperature (43◦ C) and high relative humidity (100%) for 0 (control), 24, 48, 72, 96, 120 and 144 hours (as a second factor). The results of mean comparison showed that germination percentage, rate and seedling growth of Farrokh cultivar was more than other cultivars in each aging level. Germination percentage, rate and uniformity, seedling dry weight, radicle and stem length reduced linearly with increase in the duration aging in all cultivars. Under the aging conditions, the time to beginning and ending of germination was significantly increased. Overall, it was concluded that seed quality of sunflower cultivars was seriously decreased under the aging conditions.
Zeinab Alipoor, Sohrab Mahmodi,
Volume 2, Issue 1 (9-2015)
Volume 2, Issue 1 (9-2015)
Abstract
Due to the importance of medicinal plants, understanding the seed germination response to temperature is agronomically important. A laboratory study was conducted to investigate the effect of different temperatures on seed germination of fennel, cannabis and sesame in a completely randomized design with four replications. Various constant temperatures (5, 10, 15, 20, 25, 30, 35 and 40°C) were considered. According to the results, the effect of temperature on germination rate and percentage in all species was significant. The seeds of fennel were not germinated in 5, 35 and 40 0C and seeds of sesame germinated only in 5 0C. The maximum rate of germination obtained in 30 °C for fennel and 25 °C for sesame and cannabis. The highest germination percentage of fennel and cannabis and sesame were in 20, 25 and 30 0C respectively. The lowest of germination uniformity (GU) were observed in 20 0C for fennel and in 15 0C for cannabis and sesame. Amount of seedling vigor maximized for fennel and cannabis in 250C and for sesame in the 300C. The values of length and weight of radical and plumule were enhanced with the increase of temperature and record on maximum in special temperature and then reduced slowly. The quantitative information provided by this study can be used in prediction of emergence under diverse temperature conditions. Germination of cannabis seeds occurred in a wide range of temperatures and this seed are less sensitive to temperature compared to the other two plants. Germination of fennel seeds was less, except at 200C. The sesame seeds had good germination at 15-35 0C.
Hossein Reza Badrooj, Aidin Hamidi, Amir Hossein Shirany Rad,
Volume 2, Issue 2 (2-2016)
Volume 2, Issue 2 (2-2016)
Abstract
In order to study of drought stress and normal irrigation effect on seed germination and seedling vigor of 10 oilseed rape spring genotypes, RGS003, Sarigol, Option 500, RGS006, 19_H, ORS3150-3006, ORS3150-3008, RG4403, RG405/03 and RGAS0324, an experiment was conducted as factorial, based on a completely randomized design with four replications, in the seed analysis laboratory of Seed and Plant Certification and Registration Institution at Karaj during 2013. Results indicated that RG4403 genotype by 3.498 gr. and RGS006 genotype by 3.082 gr had the highest and lowest one thousand seeds weight respectively. Seeds of Sarigol, Option500 and RGS006 genotype, respectively, by normal irrigation and drought stress, had the most primary root, shoot and seedling length and seeds of ORS3150-3008 and RGAS0324 genotypes by normal irrigation respectively had the most primary root and seedling and primary shoot and seedling dry weight. Seeds of Sarigol and RGAS0324 genotypes by normal irrigation had the highest final germination percent. The highest mean germination time, the coefficient of the velocity of germination and mean daily germination, also respectively belonged to seeds of RGS003genotype by drought stress and19-H and RGAS0324 genotypes by normal irrigation. Besides, Sarigol and RGAS0324 had the most length and weight vigor indices. Generally, results showed that drought stress at flowering and maturity duration caused a decrease in seed quality of studied genotypes, but under what conditions, some genotypes like RGS006, 19-H and ORS3150-3006 seed had relatively optimum germination quality and seedling vigor traits.
Zeinab Alipoor, Sohrab Mahmodi,
Volume 2, Issue 2 (2-2016)
Volume 2, Issue 2 (2-2016)
Abstract
In order to determinate the cardinal temperatures and investigate the effect of temperature on seed germination and seedling growth of Securigera securidaca, a study was conducted in a completely randomized design with 8 temperature treatments (5, 10, 15, 20, 25, 30, 35 and 40oC) and four replications in research laboratory of faculty of agriculture at the Birjand University in 2013. A two-segmented linear regression model was fitted between germination rate and temperatures to determine cardinal temperatures for germination. Cardinal (minimum, optimum and maximum) temperatures of germination were determined as -1, 22.5 and 40.2oC respectively. Maximum rate and percentage of germination obtained in the range of 20-25oC. Maximum of radicle length and dry weight, maximum of caulicle length and dry weight and maximum of vigor seedling were obtained in 20oC,10-20oC and 5-30oC respectively. Maximum and minimum germination uniformity (GU) was obtained in 25 and 5oC.
Ali Ghatei, Ghasem Parmoon, Zahra Amin Deldar, Hadi Ghamary,
Volume 2, Issue 2 (2-2016)
Volume 2, Issue 2 (2-2016)
Abstract
In order to evaluate the effect of hydropriming on seed germination of Vigna radiata under salinity stress, an experiment was arranged in a factorial based on a completely randomized design with three replications in the laboratory of the Agricultural College of the University of Ramin Khuzestan. Treatments were salinity stress at 4 levels (0, 3, 6 and 9 Mmhos) and hydro priming at 3 levels (1, 1.5 and 3 h) and two sizes of seed (small and large seeds) that was carried out by NaCl. Results showed that all of the germination characteristics decreased with increased salinity, but this decrement for seeds that primed at a long time, was less. Also at all characteristics, small seeds, showed better reactions than large seeds. Generally, hydropriming was improved germination characteristics of Vigna radiata under saline conditions. According to this experiment, hydropriming improved germination and establishment.
Maryam Janalizadeh, Ahmad Nezami, Hamidreza Khazaie, Hassan Feizi, Morteza Goldani,
Volume 3, Issue 1 (8-2016)
Volume 3, Issue 1 (8-2016)
Abstract
Priming of seeds by magnetic fields (magneto priming) is proposed as an ecological, useful and low-priced method for improvement of seed germination and plant emergence. In order to evaluate the germination behavior of sesame seeds affected by magnetic fields, an experiment was conducted as a completely randomized design with 22 treatments (non-exposure to magnetic fields (control) and 21 magneto priming treatments) with three replications at the college of agriculture, the Ferdowsi University of Mashhad in 2014. the seed of sesame put into a plastic bag bulky and was treated with different intensity of magnetic fields (25, 50, 75 and 100 mT) for several times (10, 20, 30, 60 and 120 minutes). For assessment of germination traits of sesame under constant magnetic field conditions, magnetic tapes with three mT strength were used in each Petri dish throughout the experiment. Results showed that magnetic fields had no significant effects on final germination percentage and mean germination time and all magnetic treatments except for 75 mT at 60 minutes indicated reducing effects on germination percentage in comparison to control. Priming of seeds with magnetic fields in 50 mT strength for 20 minutes led to the increment of germination rate compared to control treatment but all levels of 100mT treatment caused a reduction in germination rate than control. The most radicle length, seedling length and seedling vigor length index belong to 75 mT at 60 minutes treatment and the most seedling dry weight and seedling vigor weight index related to 100 mT for 20 minutes treatment. Ranking of treatments showed that exposure of seeds for one hour in 75 mT and 10 minutes in 25 mT magnetic fields strength had the best outcomes.
Marjan Diyanat, Seyyed Meisam Hosseini,
Volume 3, Issue 1 (8-2016)
Volume 3, Issue 1 (8-2016)
Abstract
In order to study the responses of redstem filaree (Erodium cicutarium L.) seed germination to temperature levels, an experiment was carried out in a completely randomized design with four replications and twelve levels of temperature (0, 3, 5, 7, 10, 12, 15, 20, 25, 30, 35, 40 0C). Results showed that the effect of treatments on germination properties of redstem was significant, so the highest percentage of germination was achieved at 150C and reduced with increasing temperature and was totally inhibited at 40ºC. Based on a linear regression between germination rate and temperature, the cardinal temperatures: minimum (Tmin), optimum (To) and maximum (Tmax) were determined: 0, 15.20 and 37.18 ºC, respectively. A significant difference was not observed in plumule length at a range of 5-30°C that showed that it is less sensitive to temperature in comparison with root length. Highest wet-dry weight was achieved at 20 °C that was not different by 10, 12 and 20°C. Above results suggest that redstem failure could grow in a wide range of growing conditions, but prefers temperate zones. The results of this study are important to understanding thermal requirements and improvement of management strategies of redstem filaree.
Farzaneh Fakhari, Hossein Sadeghi,
Volume 3, Issue 1 (8-2016)
Volume 3, Issue 1 (8-2016)
Abstract
There have been a few studies on removing the seed pods in annual medics, despite its importance. To investigate the role of pod removal on seed germination, seedling growth and antioxidant enzyme activities in annual medic (Medicago scutellata L.) Under salt stress, a factorial experiment was conducted based on a completely randomized design with four replications in 2014. Salinity at 5 levels: 0.62 (tap water as a control), 3, 6, 9 and 12 dS m-1 was applied using sodium chloride. The results showed that salt stress caused changes in the characteristics of germination, seedling growth and antioxidant enzyme activities. With increasing in salinity levels, rate and percentage of germination as well as root length, shoot length and seedling weight was significantly decreased. However, increasing salinity levels increased the activity of antioxidant enzymes. Germination and growth in seed without pod was greater than those in seeds with pod; however, there were no significant differences between antioxidant enzyme activity; except about ascorbic peroxide that was significantly higher in seed without a pod. In general, salinity reduced germination and growth and increased antioxidant enzyme activity of annual medics. The results showed that planting seeds without pod had advantages for better germination and growth, especially under saline conditions.
Shahram Nazari, Reza Deihimfard, Javad Faraji,
Volume 3, Issue 2 (2-2017)
Volume 3, Issue 2 (2-2017)
Abstract
Gaining insights into the germination and emergence patterns of weeds as well as the factors which have an impact on these patterns is beneficial for weeds management programs. In order to investigate the effect of temperature, waterlogging and salinity on germination and emergence of common lambsquarters, three separate experiments were conducted, adopting a completely randomized design with four replications. The results of the temperature experiment showed that the highest germination percentages, which were 87, 93 and 88%, were obtained in the temperature range of 15, 20 and 25°C, respectively. Maximum values of germination rate, radicle length, plumule length, and seedling dry weight were obtained at 20°C. In addition, optimum seed vigor index of 796.29 was observed at 20°C. The results of the Regression Model showed that germination percentage decreased with an increase in waterlogging duration and halted after 8 and 10 days of consecutive waterlogging. Mean comparisons revealed that radicle and plumule length, seedling dry weight and vigor index decreased significantly by increasing the period of waterlogging. Salinity adversely affected germination percentage, germination rate and seedling dry weight of common lambsquarters. These factors were at their highest amounts in the control and 50 mM NaCl treatments. A downward trend was observed in these factors as NaCl concentrations increased and finally the germination process stopped in concentrations ranging from 300 to 400 mM NaCl. Radicle and plumule length also decreased from 10.51 and 9.23 cm in the control treatment to 3.57 and 2.47 cm at 250 mM NaCl. Overall, the results revealed that the maximum seed vigor index of 851.84 was obtained in the control treatment and seed vigor halted when the salinity level increased to more than 250 mM NaCl. Finally, the results showed that optimum temperature for germination of common lamsquarters was 20 °C and the existence of salinity and waterlogging stress can decrease growth indices of this weed at germination and seedling stages.
Mahmod Reza Tadayon, Mohammad Rahimi,
Volume 3, Issue 2 (2-2017)
Volume 3, Issue 2 (2-2017)
Abstract
The purpose of this study was to evaluate the effect of Nano TiO2 and Nano CNT on some germination indices and growth parameters of some hulled barley cultivars. The experiment was conducted in a laboratory at Shahrekord University during 2014. The study was a factorial, adopting a completely randomized design with four replications. Treatments consisted of titanium dioxide nanoparticles and carbon nanotube (CNT) in four concentrations (0, 10, 30 and 60 mg.l-1) which were applied to seeds of hulled barley cultivars such as Bahman, Makoii and Nosrat. The traits measured were the dry weight of seedling and seedling length, germination percentage, germination rate, mean germination time, the percentage of resistance of radical, seedling vigor length and weight index. The results showed that carbon nanotubes treatments with 60 mg.l-1 had a significant impact on germination percentage, germination rate, seedling vigor length and weight index and seedling dry weight, as compared with other treatments. In this experiment, the highest percentages of resistance of radicle, seedling vigor weight index and seedling dry weight of Nosrat Cultivar were obtained under 60 mg.l-1 carbon nanotube treatment, which was 70, 122.2 and 64.9%, respectively, as compared with control treatment. In addition, 60 mg.l-1 carbon nanotube treatment increased the seedling vigor of Makoii cultivar by 39.8%, as compared with the control treatment. The findings were that in terms of seedling vigor length and weight index, seedling length, seedling dry weight and the percentage of resistance of radical, the Nosrat Cultivar showed better morphological characteristics than Bahman and Makoii cultivars, under laboratory conditions.
Zahra Ajribzadeh, Hamidreza Balouchi, Alireza Yadavi, Amin Salehi,
Volume 4, Issue 1 (9-2017)
Volume 4, Issue 1 (9-2017)
Abstract
In order to evaluate the allelopathic effect of aqueous extract of nine weeds species on Anise (Pimpinella anisum) seed germination and seedling growth characteristics, an experiment was conducted in the Laboratory of Yasouj University in 2014. This experiment was carried out as factorial with the aqueous extracts of nine weeds species, as the first factor, in different concentrations (10, 20, 30, 40 and 50 g.l-1), as the second factor, in a completely randomized design with four replications. The results showed that interactions between different weed aqueous extracts and concentrations had a significant impact on the germination percentage and rate, root and shoot length and weight, and vigor of anise seeds at 1% probability. The most allelopathic effects on germination percentage and rate were observed in 10 g/l of the aqueous extract of Cowherb and Corn Buttercup, in 20 to 30 g/l of Corn Buttercup and Whitetop, and in 40 to 50 g.l-1 of Corn Buttercup, Whitetop, Wild lettuce and Cowherb, which completely stopped seed germination in Anise.
Highlights:
Highlights:
- Allelopathic effects of 9 common weedy types on germination of Anise Seed were studied in Kohgiluyeh and Boyerahmad.
- The effect of different concentrations of aqueous extracts of weeds on germination characteristics of anise was studied.
Roozbeh Farhoudi, Zahra Khodarahmpour,
Volume 4, Issue 1 (9-2017)
Volume 4, Issue 1 (9-2017)
Abstract
The present study was conducted to evaluate the impact of drought and salinity stresses on germination, seedling growth and cell membrane stability of anise (Pimpinella anisum) and fennel (Foeniculum vulgare) in two separate experiments, using a completely randomized design with three replications in Islamic Azad University, Shoushtar Branch in 2013. Drought stress was applied by PEG 6000 and included 0, -2, -4, -6 and -8 bar and salinity treatments were 0, 40, 80 and 120 mM NaCl solutions. Salt and drought stresses significantly reduced germination percentage and seedling fresh weight of anise and fennel, but increased mean germination time and seedling electrical leakage. The results showed that the highest salinity and drought stresses levels increased seedling electrical leakage of anise by 83% and 76% compared with the control conditions. Moreover, seedling electrical leakage of fennel increased up to 77% and 75%, as compared with the control. The results showed that at germination stage, salt and drought stresses increased cell membrane damage, but decreased anise and fennel seedling growth.
Highlight:
Highlight:
- Variations in concentration and the rate of destruction of seedling membranes of anise and fennel plants were studied in response to environmental stresses.
- The germination characteristics of anise and fennel were studied under salt and drought stresses.
Goudarz Ahmadvand, Masoume Dehghan Banadaki, Javad Alimoradi, Sara Goudarzi, Sasan Ardalani,
Volume 4, Issue 2 (3-2018)
Volume 4, Issue 2 (3-2018)
Abstract
Salt and drought are two major environmental stresses that affect growth and development of plants. In order to study the effects of sodium chloride and polyethylene glycol (6000) on germination characteristics and early seedling growth of redroot pigweed (Amaranthus retroflexus), two completely randomized designs with 4 replications were conducted at Weed Research Laboratory of Bu-Ali Sina University, Hamedan in 2015. The treatments were salt and drought stress as osmotic potential at six levels (zero (control), -2, -4, -6, -8 and -10 bar). The results showed that when stress increased, germination percentage, germination speed, radicle and plumule length, seedling length and seedling vigor index decreased significantly (p≤ 0.05). With an increase in the intensity of salt and drought stress from zero to -10 bars, redroot pigweed seed germination reduced about 96 and 100 percent, respectively, compared with the control. The highest seedling length in both stresses was observed in the control (7.71 cm) and by increasing stress intensity to -10 bars in both salinity and drought stresses, seedling length was reduced to 1.52 cm and 0 cm, respectively. Fitting of the three-parameter logistic model provided a successful estimation of the relationship between salt and drought stress levels and germination percentage of redroot pigweed as well as germination speed. This model showed that salinity and drought stress at -3.58 and -3.75 bars caused a 50% reduction in maximum germination percentage of redroot pigweed. In addition, 50% decrease in germination speed caused by salinity and drought stress was observed in -2.58 and -2.88 bars, respectively.
Highlights:
- The germination characteristics of redroot pigweed were studied under salt and drought stresses.
- Drought stress reduced germination percentage of redroot pigweed.
Maryam Mokhtari, Sina Fallah,
Volume 6, Issue 1 (9-2019)
Volume 6, Issue 1 (9-2019)
Abstract
Extended Abstract
Introduction: In order to take more advantage of the spring growing season, the mechanisms of germination of spring plants are of great importance at temperatures lower than the optimum temperature. Since one of the ways to reduce damage due to low temperature is enhancing the seedling antioxidant system, in this study the effects of salicylic acid and gibberellin on germination and antioxidant system of pumpkin (Cucurbita pepo) seeds were investigated under low temperatures.
Materials and Methods: A factorial experiment including four concentrations of gibberellin (0, 250, 350 and 450 mg/L), four concentrations of salicylic acid (0, 0.5, 1 and 1.5 mM) and three temperature levels (8, 11 and 14 °C) was performed with a completely randomized design within controlled conditions and six replications at Shahrekord University in 2017. The seeds were immersed in containers containing solutions of 0, 250, 350 and 450 mg/L of gibberellin and solutions with 0, 0.5, 1, and 1.5 mM salicylic acid, were placed in a growth chamber for 24 h under dark conditions at 15 °C. Then the seeds were washed at the desired temperatures, and the germination was recorded every 24 hours based on the 2 mm of radicle length. At the end of the eighth day, after the separation of normal and abnormal seedlings, 20 normal seedlings were selected from each petri dish. Following that, the germination rate, germination percentage, soluble protein, malondialdehyde, superoxide dismutase, guiacol peroxide enzyme, and catalase enzyme were measured. Comparison of means was conducted by the least significant difference test at the 0.05 probability level.
Results: The results showed that none of the treatments used at 8 °C helped germination of the plant and, therefore, 8 °C treatment was removed from the experiment. At the temperature of 11 ° C, the use of salicylic acid 1 mM and at 14 °C, the use of gibberellin 350 mg/L showed the maximum germination rate and germination percentage, compared with the control. At 11 °C, the activity of antioxidant enzymes was more affected by gibberellin hormone so that the highest activity of superoxide dismutase enzyme was observed in 350 mg/L and the highest activity of catalase and guaiacol peroxidase enzymes and the lowest amount of soluble protein were observed in gibberellin 250 mg/L. The salicylic acid hormone was more successful at 14 ° C. The salicylic acid 1.5 mM increased the activity of superoxide dismutase enzyme; and salicylic acid 0.5 mM increased the activity of catalase and salicylic acid 1 mM improved the activity of guiacol peroxidase. This hormone also succeeded in reducing the amount of soluble protein.
Conclusion: In this experiment, seedling tolerance at low temperatures was confirmed by gibberellin and salicylic acid treatments. It is generally concluded that the use of gibberellin and salicylic acid increases the activity of antioxidant enzymes and, as a result, makes pumpkin (Cucurbita pepo) seedlings tolerant to low-temperature stress, and thus, can ameliorate the effect of possible chilling on growth of this crop at the beginning of the season.
Highlights:
- Gibberellin and salicylic acid treatments make pumpkin seedling tolerant to low temperatures.
- Application of gibberellin and salicylic acid increases the activity of antioxidant enzymes.
- By using gibberellin and salicylic acid, the effect of possible chilling can be reduced at the beginning of the growing season.
Narjes Hojati Fahim, Mohamad Sedghi, Mehrdad Chaeichi, Rraouf Seyed Sharifi,
Volume 6, Issue 1 (9-2019)
Volume 6, Issue 1 (9-2019)
Abstract
Extended Abstract
Introduction: Iran is located in the arid and semi-arid regions of the world with an average rainfall of 240 mm per year, which requires rethinking of the adoption of methods. One of the alternative ways is to use organic and biological fertilizers. Biological fertilizers are considered as the main and the most important factor in the integrated management of plant foods for sustainable agriculture as they play an important role in product improvement and efficiency. Therefore, this research was carried out with the aim of investigating the effect of seed pre-treatment with some organic and biological fertilizers in rainfed wheat.
Materials and Methods: A factorial experiment with three replications was conducted at the Laboratory of Seed and Plant Certification and Registration of the Center of Agriculture and Natural Resources Research located in Hamedan. Seed inoculation was considered in 5 levels (Seafull, Disper Root Gs., Bio-Health, Trichodermin and control) on 8 different rainfed wheat cultivars (Azar-2, Hashtrood, Baran, Rasad, Owhadi, Sardari, Takab and Homa). First, the seeds were disinfected with sodium hypochlorite, and were then cultured in special containers and were placed in the germinator at 20 ° C. After 4 and 8 days, the number of germinated seeds was counted. Germination seeds were counted in each treatment and germination indices such as germination rate, average daily germination, coefficient germination rate, longitudinal vigor index, weight vigor index, seedling fresh weight, root length/shoot ratio, shoot fresh weight, root fresh weight, seed reserve utilization rate, seed reserve utilization efficiency, fraction utilization seed reserve, seed dry weight were calculated.
Results: With application of different levels of fertilizer, the rate of germination treatment Homa×Bio-Health (155%), average daily germination treatment Hashtrood×Bio-Health (69%), coefficient germination rate treatment Owhadi×Disper Root Gs (60%), longitudinal vigor index treatment Owhadi×Bio-Health (108%), weight vigor index treatment Homa×Bio-Health (64%), root fresh weight treatment Hashtrood×Disper Root Gs (106%), shoot fresh weight treatment Hashtrood×Seafull (23%), seedling fresh weight treatment Homa×Bio-Health (42%), root length/shoot ratio treatment Owhadi×Trichodermin (75%), seed reserve utilization rate treatment Homa×Bio-Health (118%), and fraction utilization seed reserve treatment Homa×Bio-Health (119%) increased, compared with the control. In addition, the application of Bio-Health fertilizer and Hashtrood cultivar had the highest amount in almost all the mentioned attributes
Conclusion: Investigation of the different levels of fertilization showed that in most of the indices related to germination and heterotrophic growth of seedling, pretreatment with Bio-Health biofertilizer had a significant difference with other fertilizer levels.
Highlights:
- Evaluation of germination indices for recent cultivars of rainfed wheat, released by rainfed Research Institute of Iran.
- Investigating and comparing fertilizers with various multifactorial compounds (fungi and bacteria) and with each other.
- Conducting research on commercial compounds and comparison of live biochemical and non-organic matters in a single experiment.
Effects of TiO2 Nanoparticles on Germination and Primary Growth of Mountain Ash (Sorbus luristanica)
Vahid Sayedena, Babak Pilehvar, Kambiz Abrari-Vajari, Mehrdad Zarafshar, Hamid Reza Eisvand,
Volume 6, Issue 1 (9-2019)
Volume 6, Issue 1 (9-2019)
Abstract
Extended Abstract
Introduction: Production of nanoparticles and their use are on the rise in different areas of plant science. However, in spite of their increasing production, there is limited information about their effects on plant biology. In the current study, the potential of TiO2 nanoparticles was investigated for the purpose of improving seed germination of Sorbus luristanica and then subsequent effects of nanoparticles on the growth and biomass of the plants were determined.
Materials and Methods: Seeds of S. luristanica were collected from its natural stands. The seeds were primed with different concentrations of 0, 75, 150, 250, 350 and 500 TiO2 nanoparticles miligeram per liter for 24 h. The treated seeds were placed in wet sand at room temperature for 2 weeks and then in cold for 3 months. The expriment was set as a completely randimized design with 4 replications. Aftre 3 months of stratification in moistened sand, the stratified seeds were put in the germinator and with the appearance of seed germination signs, germination data were recorded daily during 22 days. At the end of the seed germination experiment, some germination parameters such as seed germination percentage, seed vigority and mean time to germination were calculated. Moreover, some growth and biomass parameters including leaf number, plant height and dry and fresh biomass of leaf, stem as well as roots were measured. In addition, scaning electron microscopic (SEM) was used for observation of presence and adhesiveness of TiO2 nanoparticles on the seed coat.
Results: Based on the results, all the germination parametres including seed germination percentage, seed vigoroty and mean germination time were improved by the TiO2 nanoparticles treatments. In addition, 500 mg.L-1 treatment considerably improved seed germination characteristics. The peresence of TiO2 nanoparticles on the treated seeds and lack of the nanomatreials on the conrtol seeds were obsereved by scaning electron microscopic pictures. The One-way ANOVA showed that 75 mg.L-1 treatment was more succesful for improving the grwoth (such as shoot length) and biomass production (fresh and dry biomass of leaf, stem and root and total biomass as well).
Conclusion: It can be concluded that priming of the seeds of this species with different concentrations of TiO2 nanoparticles leads to improvement of seed germination and growth and biomass parameters. However, the patterns of effects were different in each phase. Therefore, the objectives should be formulated first and then the best concentration should be chosen. It seems that with appropriate concentrations, nanoparticles can be useful for breaking seed dormancy and production of the species. Given the promising resutls of 150 mg.L-1 treatment, it can represent a successful treatment for breaking seed dormancy and seedling production of S. luristanica.
Highlights:
1- Study of seed germination of Sorbus luristanica for the first time
2- Using Nano-materials and their potentials in breaking seed dormancy and improving the species germination
3- Using SEM in order to study presence and adhesiveness of nanoparticles on the seed coat
Mahsa Nazer, Seyed Mohammadreza Ehteshami, Masoumeh Salehi, Ali Kafighasemi,
Volume 6, Issue 1 (9-2019)
Volume 6, Issue 1 (9-2019)
Abstract
Extended Abstract
Introduction: Guar (Cyamopsis tetragonolob) which belongs to fabaceae and leguminosae families is self-pollinating and is indigenous to India and Pakistan. This plant is suitable for growth in dry, damp and sandy soils, and can tolerate saline and relatively alkaline soils. Determining the best time for harvesting seeds and its timing with maximum quality is one of the important issues in the field of seed production management. The changes in the physiological quality of seeds occur during development until seed treatment. The use of high-quality seeds plays an important role in the final yield of crops. The purpose of this study was to determine the most suitable time for harvesting seeds because seed moisture content at harvest time is one of the most important factors affecting seed quality.
Materials and Methods: This research was carried out in July 2013 at Iraqi Research Station in Gorgan, Golestan, Iran in a split-plot design with four replications. The treatments consisted of the time of harvesting the seeds from the mother's base in six stages (with different moisture content) and harvesting places (upper, middle, and bottom pods) so that, starting from the podding, every 7 days, the seeds were removed from the base. The mother was harvested and such traits as germination rate, germination percentage, seedling vigor, seed moisture content, and alpha-amylase activity were calculated. In this research, a logistic model was used to study the changes in germination percentage, germination rate, seedling vigor index and straw index during seed dressing on Guar.
Results: The results of this study showed that the hypothesis that seed reaches its maximum quality at the end of the period of seed filling is confirmed, suggesting that with a moisture content of 30 and 14% in late seed filling period, Guar seeds have the highest quality. In general, the results of the present study confirm that the stages of development and management of Guar seeds on maternal basis have an impact on its quality. In the early stages of growth (humidity 85, 80 and 62 percent), due to prematurity and lack of essential seed structures, the qualitative traits of germination percentage, germination rate, seedling vigor, and strawberry index were low, and with the evolution of essential structures and reduced seed moisture content (58, 30 and 14%), the quality traits increased.
Conclusions: In general, it can be concluded that the best timing for harvesting Guar seeds with the highest quality in Golestan province is when seed moisture reaches 14% or 100 days after planting, and the pods formed at the bottom are the ones with the highest quality.
Highlights:
- To investigate the qualitative indices of Guar seed on the mother plant during seed filling period
- To determine the best harvest time and its adaptation with the highest quality of Guar seed
Marzie Soltani Alikooyi, Ali Abbasi Surki, Mohsen Mobini Dehkordi, Shahram Kiyani,
Volume 6, Issue 2 (3-2020)
Volume 6, Issue 2 (3-2020)
Abstract
Extended Abstract
Introduction: Salinity is one of the most serious abiotic stresses, causing instability in germination and seed emergence due to low osmotic potential and ionic toxicity. Development of simple and low-cost biologic methods is essential for short-term management of salt stress. The use of plant growth-promoting rhizobacteria increases the rate and uniformity of germination. This research aimed to investigate the effect of bacterial growth-promoting bacteria on the germination and seedling growth indices of alfalfa c.v. Hamedani in different salinity levels.
Materials and Methods: A CRD factorial experiment with four replications was conducted in Seed Science and Technology Laboratory of Shahrekord University in 2016. The first factor consisted of 6 salinity levels 0, 2.5, 5, 7.5, 10 and 12.5 dS/m created with sodium chloride, and the second was four levels of bacterial pre-treatment: no inoculation with bacteria and biopriming, inoculation of alfalfa seeds with Acinetrobacter calcoaceticus PTCC 1318, Bacillus megaterium PTCC 1250 and Enterobacter aerogenes PTCC 1221. The seeds were treated with bacteria and placed at a 20 °C growth chamber. They were then irrigated with desired solutions depending on the salinity treatment. Germinated seeds were counted daily and the parameters of germination percentage and rate, seedling length, seedling dry weight, vigour index I, II and allometric coefficient were calculated after 10 days.
Results: Salinity levels higher than 10 dS/m reduced germination indices and seedling growth of alfalfa. The highest reductions were obtained for 12.5 ds/m salinity level versus control for germination percentage (10.81%), germination rate (49.48%), plumule and radicle length (13.30% and 28.88% respectively) and vigor index I and II, which were 30.27% and 6.28%, respectively. The seed treated with A. calcoaceticus was able to tolerate salinity stresses more than others. For example, the reduction for the seed treated with A. calcoaceticus was only 4%, compared with non-stressed control. In salinity conditions 2.5 and 5 dS/m, the highest rate of germination was obtained, using A. calcoaceticus bacteria. In addition, the seeds treated with E. aerogenes showed higher stability at different levels of salinity for seedling length traits. The highest vigour index related to the use of A. calcoaceticus in salinity was 7.5 ds/m.
Conclusions: A. calcoaceticus had a significant role in reducing the negative effects of salinity on germination percentage and rate, vigour index I and II and allometric coefficient while E. aerogenes bacteria were more effective in reducing negative effects of salinity on seedling length and dry weight.
Highlights:
- Acinetrobacter calcoaceticus bacterium increased the percentage and rate of germination of alfalfa seeds under salt stress.
- Enterobacter aerogenes bacteria efficiently adjusted the negative effects of salinity on alfalfa seedlings length and dry weight.
Mohammad Ghayour, Majid Taherian, Sadegh Baghban, Saeed Khavari,
Volume 6, Issue 2 (3-2020)
Volume 6, Issue 2 (3-2020)
Abstract
Extended Abstract
Introduction: The effect of environmental factors on the developmental stages of a plant causes the planting date to vary from one region to another. Temperature is a very important factor in the maximum percentage germination and germination rate. Priming improves germination rate, brings about the uniformity of germination and reduces seed susceptibility to environmental factors. The purposes of this experiment were to study the effects of priming treatments at different temperatures on the germination characteristics of Hibiscus sabdariffa under laboratory conditions, to investigate priming treatments on different planting dates and to compare early planting dates on the farms.
Materials and Methods: The experimental study was carried out as a factorial experiment in a completely randomized design with four replications in the Laboratory of Seed Technology of Kashmar University Jihad. The first factor is five primings (Concentrations of ZnSO4 (10 mM), Humic acid (2.5 cc) and the combination of Humic acid and Zinc sulfate, Biological materials (Pota Barvar 2), no treatment (control) and the second factor is five levels of temperature: 10, 12, 14, 16 and 18 °C. Field studies were carried out in Agricultural and Natural Resources Research Center of Kashmar in three separate experiments in 2018. The research was carried out in a completely randomized block design with three replications on three planting dates (March 25th, April 15th, and May 4th, 2018). In each experiment, priming treatments were applied similarly to field experiments.
Results: The results of the current experimental study showed that temperature, priming and temperature interaction with priming had a significant effect on all the traits studied. The highest percentage and rate of germination were observed at 18 °C and priming with Pota Barvar 2. The results also showed that planting date had a significant effect on all the traits studied in the field experiment. Priming showed a significant difference only in stem fresh weight. The interaction effect of priming and planting date was not significant for the traits studied. The highest germination percentage was obtained on April 15’s planting date. By planting later than March 25 to 15 April, a decrease of 74% was observed in the rate of germination. Among priming treatments, the combination of Zinc Sulfate and Humic acid showed a significant superiority, compared with other treatments.
Conclusion: The findigns suggest that due to the high sensitivity of seed germination of sour tea at low temperatures, the planting date in each area should be carefully chosen so that it does not coincide with temperatures below 18 °C.
Highlights:
- Evaluation of the effect of seed priming treatment at different temperatures on germination characteristics of Roselle under laboratory conditions.
- A different priming treatment leads to increases in germination characteristics of Roselle.
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Seed Science and Technology, Seed Physiology, Seed Ecology, Modeling, Seed Health, Germination Characteristics, Seed Dormancy, Seed Ageing, Seed Storage, Seed PrimingVote
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