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Narjes Hojati Fahim, Mohamad Sedghi, Mehrdad Chaeichi, Rraouf Seyed Sharifi,
Volume 6, Issue 1 (9-2019)
Abstract



Extended Abstract
Introduction: Iran is located in the arid and semi-arid regions of the world with an average rainfall of 240 mm per year, which requires rethinking of the adoption of methods. One of the alternative ways is to use organic and biological fertilizers. Biological fertilizers are considered as the main and the most important factor in the integrated management of plant foods for sustainable agriculture as they play an important role in product improvement and efficiency. Therefore, this research was carried out with the aim of investigating the effect of seed pre-treatment with some organic and biological fertilizers in rainfed wheat.
Materials and Methods: A factorial experiment with three replications was conducted at the Laboratory of Seed and Plant Certification and Registration of the Center of Agriculture and Natural Resources Research located in Hamedan. Seed inoculation was considered in 5 levels (Seafull, Disper Root Gs., Bio-Health, Trichodermin and control) on 8 different rainfed wheat cultivars (Azar-2, Hashtrood, Baran, Rasad, Owhadi, Sardari, Takab and Homa). First, the seeds were disinfected with sodium hypochlorite, and were then cultured in special containers and were placed in the germinator at 20 ° C. After 4 and 8 days, the number of germinated seeds was counted. Germination seeds were counted in each treatment and germination indices such as germination rate, average daily germination, coefficient germination rate, longitudinal vigor index, weight vigor index, seedling fresh weight, root length/shoot ratio, shoot fresh weight, root fresh weight, seed reserve utilization rate, seed reserve utilization efficiency, fraction utilization seed reserve, seed dry weight were calculated.
Results: With application of different levels of fertilizer, the rate of germination treatment Homa×Bio-Health (155%), average daily germination treatment Hashtrood×Bio-Health (69%), coefficient germination rate treatment Owhadi×Disper Root Gs (60%), longitudinal vigor index treatment Owhadi×Bio-Health (108%), weight vigor index treatment Homa×Bio-Health (64%), root fresh weight treatment Hashtrood×Disper Root Gs (106%), shoot fresh weight treatment Hashtrood×Seafull (23%), seedling fresh weight treatment Homa×Bio-Health (42%), root length/shoot ratio treatment Owhadi×Trichodermin (75%), seed reserve utilization rate treatment Homa×Bio-Health (118%), and fraction utilization seed reserve treatment Homa×Bio-Health (119%) increased, compared with the control. In addition, the application of Bio-Health fertilizer and Hashtrood cultivar had the highest amount in almost all the mentioned attributes
Conclusion: Investigation of the different levels of fertilization showed that in most of the indices related to germination and heterotrophic growth of seedling, pretreatment with Bio-Health biofertilizer had a significant difference with other fertilizer levels.
 
 
Highlights:
  1. Evaluation of germination indices for recent cultivars of rainfed wheat, released by rainfed Research Institute of Iran.
  2. Investigating and comparing fertilizers with various multifactorial compounds (fungi and bacteria) and with each other.
  3. Conducting research on commercial compounds and comparison of live biochemical and non-organic matters in a single experiment.

Hadi Pirasteh-Anosheh,
Volume 7, Issue 1 (9-2020)
Abstract



Extended abstract
Introduction: Camelthorn (Alhagi maurorum) has a high tolerance to salt and water stresses, and its forage quality is greater than cereal straw and is equal to alfalfa. Seeds of camelthorn do not germinate easily due to the hard seed coat. Therefore, camelthorn cultivation as an agricultural crop needs more research, especially on breaking seed dormancy and increasing germination. Despite numerous studies about camelthorn as a weed, there are few studies on evaluating agronomic factors of camelthorn as a field crop. Hence, in this study, some aspects of germination and salinity tolerance of camelthorn were examined.
Materials and Methods: In this research, different aspects of camelthorn germination were examined at the National Salinity Research Center in 2018. In the first experiment, different pretreatments including control, sandpaper, hydro-priming, hot water, and sulphuric acid were assessed. With the selection of sulphuric acid as the best treatment, varying durations (0, 5, 10, 15, 20, and 25 min) and concentrations (98% and 75%) of sulphuric acid priming were compared in the second and third experiments. In the fourth experiment, the seeds collected in 2018 were compared with the seeds collected in 2017 and 2016. The effect of different salt stress levels (0, 6, 12, 18, 24, 30, and 36 dS m-1) was evaluated on camelthorn germination and early growth in the fifth experiment. In the sixth experiment, lighting regimes including continuous dark, continuous light, and an alternative period of dark- light were examined and in the seventh experiment sibling factors (together and individual seeds) were evaluated. Seed germination and seedling length were calculated and salinity tolerance threshold levels and 50% reduction threshold were estimated.
Results: The results of the first experiment showed that the highest germination percentage was obtained in sulphuric acid priming (56.6%), which was six folds greater than the control. In the second experiment, it was shown that the highest germination percentage (81.1%) and seedling growth (5.7 cm) was observed in sulphuric acid priming 98% for 25 min. Important note was that these results were related to domestically produced sulphuric acid, and the highest germination and seedling growth were observed in 75% concentration for the imported sulphuric acid. In the fourth experiment, it was found that at least 3 years of seed longevity had no significant effect on seed germination. Considerable losses in germination and growth were observed from 30 dS m-1 salinity level; however, germination did not completely stop even at 36 dS m-1 salinity. In the sixth and the seventh experiments, it was found that there were no significant differences between seeds germination in the continuous dark, continuous light, and an alternative period of dark- light as well as between individual and together seeds. So, camelthorn seed is not photoblastic and had no negative sibling factor.
Conclusions: Generally, it was concluded that the best method for improving germination is priming with sulphuric acid 98% (internal) or 75% (imported) for 25 min. According to achievement of high germination in sulphuric acid pre-treatments (chemical scarification), it seems that seed dormancy in camelthorn is presumably physical. Furthermore, although the salinity tolerance threshold of this plant is estimated 14.2 dS m-1, it can germinate minimally even in very higher salinity. Light salt stress not only decreases the germination of this plant but also is necessary for growth promoting. Based on the high salinity tolerance of camelthorn in the germination stage, its cultivation in haloculture systems is recommended for more investigation.

Highlights:
1- Sulphuric acid 98% priming for 25 min led to breaking seed dormancy and acceptable camelthorn germination.
2- In moderate salinity, germination was not significantly changed and seedling growth was promoted compared with the non-stress conditions.
3- Salinity tolerance threshold level of camelthorn was estimated 14.2 dS m-1.


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