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Showing 11 results for Sharifi
Effect of seed priming on growth features of Spinach (Spinacia oleracea) in sub-tropical regions
Gholam Reza Sharifi-Sirchi,
Volume 1, Issue 1 ((Spring and Summer) 2014)
Abstract
In order to extend the cultivation season and increase in growth ability in tropical regions, the effect of priming media, SA, NaCl, PEG, water and vitamin B1 with chemical elimination of pericarp by sulphuric acid, evaluated on growth characters of spinach under high temperature stress (25, 30, 35 °C). Results showed that PEG and vitamin B1 pretreatment after scarification with sulphuric acid 9 M increased germination percentage under 25 and 30°C. Also, for the propose of considering the effect of different priming media on spinach see at the optimum temperature of winter, seeds were sown in greenhouse and farm in Minab city. Results of greenhouse cultivation showed that hydro-primed seed and seed which primed with vitamin B1 and PEG had the highest emergence. In the field, the primed plant treated with vitamin B1, NaCl and distilled water had the highest yield. Therefore, this study suggests proofing laboratory results some experiments should be performed in farm filed with hotter temperatures by using vitamin B1 and PEG pretreatments.

Effect of Nano Zinc Oxide on the Kernel Filling Period in Sunflower (Helianthus annuus) Cultivars
Mohammad Sedghi, Farzaneh Hasani, Raouf Seyed Sharifi,
Volume 1, Issue 2 ((Autumn & Winter) 2015)
Abstract
Application of zinc can improve the tolerance and resistance of plants especially sunflower to environmental stresses and be effective on kernel set and yield. To investigate the effects of zinc oxide nanoparticles on the length, effective period, the rate of kernel filling and kernel weight in sunflower cultivars, a factorial experiment based on randomized complete block design with three replications was conducted at the University of Mohaghegh Ardabili, Ardabil, Iran. Treatments were three sunflower cultivars as Aline 191, Aline 122 and Farrokh and three concentrations of nano zinc oxide including 0, 0.5 and 1 g lit-1 which sprayed at three stages. Results showed that interaction of cultivar and nano zinc oxide was significant on kernel filling rate, the effective period of kernel filling, maximum kernel weight and duration of kernel filling. The rate of filling and maximum kernel weight increased with 0.5 g lit-1 spraying of Nano zinc oxide in Aline 191 and 122, but in Farrokh cultivar was significantly reduced in comparison to the control. Also, no significant difference was found between 0, 0.5 and 1 g lit-1 of nano zinc oxide on kernel filling rate, effective kernel-filling period and duration in Farrokh cultivar. In conclusion, foliar application of zinc oxide nanoparticles led to the improvement of kernel weight and their filling parameters and the more appropriate response was observed in foreign cultivars for this treatment, while final kernel weight decreased in Farrokh cultivar. Therefore, it can be used 0.5 g lit-1 of nano zinc oxide for increasing grain yield in foreign cultivars of Aline.

Study of Seed Dormancy in Seven Medicinal Species from Apiaceae
Hamid Sharifi, Mohammad Khajeh-Hosseini, Mohammad-Hassan Rashed-Mohassel,
Volume 2, Issue 1 ((Spring and Summer) 2015)
Abstract

Seeds of seven species of medicinal plants collected from the natural habitat in Lorestan province in summer 2011. Germination test carried out in a completely randomized design with four replications of 25 seeds in H2O. Species of Smyrnium cordifrolium, Kelussia odoratissima, Dorema aucheri and Ferulago angulata had no germination while Heracleum persicum, Bunium luristanicum and Falcaria vulgaris showed germination of 30, 96 and 97% respectively. Different treatments of breaking dormancy applied to the species with germination below 30% [moist-chilling for periods of 2, 4, 6, 8, 10 and 12 weeks, with two concentrations of 250 and 500 ppm of gibberellic acid, a combination treatment (gibberellic 250 ppm + 4 weeks moist-chilling and gibberellic acid 500 ppm + moist-chilling for 4 weeks) and potassium nitrate 2 g/l]. The results showed that moist-chilling was the most effective treatments to break seed dormancy of Heracleum persicum (6 weeks), Dorema aucheri (12 weeks), Kelussia odoratissima (12 weeks) and Ferulago angulata (12 weeks). Therefore, based on their reactions to the treatments, dormancy of Kelussia odoratissima and Ferulago angulata could be classified as deep physiological dormancy and species of Dorema aucheri and Heracleum persicum intermediate physiological dormancy type.


Effect of Seed Coat Color and Different Treatments on Seeds Dormancy and Germination Characteristics of Mustard (Sinapis arvensis)
Hamid Sharifi, Morteza Goldani,
Volume 2, Issue 2 ((Autumn & Winter) 2016)
Abstract

Seed coat color as a perfect and simple index is effective in water absorption, seed dormancy and germination. The mustard plant produces seeds with different coat color. So, in order to investigate the effect of seed coat color on germination rate, the percentage of germination and dormancy of Mustard (Sinapis arvensis L.) seeds an experiment was carried out based on a completely randomized design with four replications. Treatments were included two types of seed coat color (brown and black) and dormancy breaking treatments (Control, Potassium nitrate 0.2%, GA3 250, GA3 500 ppm and one, two and three weeks prechilling). The results showed that the differences between germination indices traits in brown and black seeds were significant. So that, the germination rate and germination percent in black seed were greater than brown seeds, but the duration of dormancy in brown seeds was greater than black seed. One week prechilling treatment had the most effect on breaking dormancy. So that germination percentage and germination rate for black seed was 75% and 0.54 respectively and these amounts for brown seeds were 58% and 0/43 respectively. Potassium nitrate and GA3 (250 and 500 ppm) reduced germination rate and germination percent in both types of seeds. In addition, water absorption percent in brown seeds was more than black seeds. The final results showed that heteromorphic seeds of Mustard have variation in duration of dormancy, germination and water absorption percent.


Effects of Hardening and Quality of the Seeds From Different Positions of Inflorescence on the Storability of Pot Marigold
Effat Ghaviazm, Mohammad Sedghi, Raouf Seyed Sharifi,
Volume 2, Issue 2 ((Autumn & Winter) 2016)
Abstract

Marigold (Calendula officinalis) is a medicinal plant belonging to the Asteraceae family, which seems necessary the review of its various features especially the improvement of reproduction in order to the development of vegetation and extraction of raw pharmaceutics materials. In order to study the effects of seed hardening on the quality of seeds harvested from different positions of inflorescence on the storability of pot marigold, an experiment conducted at the University of Mohaghegh Ardabili in 2011. Seeds hardened before planting with CaCl2 2% and distilled water. At harvest, the seeds collected from both inner and outer regions of the inflorescence from storage experiments in the laboratory. Harvested seeds stored for six months at 40°C. Then an experiment conducted as factorial based on a completely randomizes design with four replications at the laboratory. Results showed that the highest germination percentage (96%), rate (3.91 day-1), radicle length (5.71 cm) and seed residual dry weight (0.061 g) were related to outer positioned seeds. The highest plumule length (4.79 cm) and seedling dry weight (0.016 g) obtained from outer seeds hardened with distilled water. In conclusion, for best stability results of pot marigold seeds, it is recommended to harden seeds before planting with water and harvest outer seeds from the inflorescence.

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The Effect of Pre-Chilling and Gibberellic Acid on Breaking Seed Dormancy of Two Medicinal Plants Species Silybum Mrianum and Citrulus Colocynthis
Almas Nemati, Hamid Sharifi, Mohammad Gerdakaneh, Zeynab Sharifi,
Volume 3, Issue 1 ((Spring and Summer) 2016)
Abstract

The seeds of two species (Citrulus colocynthis) and (Silybum mrianum) gathered from natural habitat located in the Koohdasht city (Lorestan province) and transferred to Kermanshah Agricultural and Natural Resources Research and Education Center in the summer of 2014. For each species, separate experiments in a completely randomized design with 14 treatments and 4 replications were done. Treatments included moist-chilling time (control, 10, 20, 30, 40, 50, 70 and 90 days at 4 °C), gibberellic acid concentration (200, 400, 600 and 800 PPM) and integrated treatment of gibberellic acid 400 ppm with moist-chilling for 30 days and 70 days. The results showed that for both species increasing of moist-chilling duration and concentration of gibberellic acid significantly increased germination percentage, germination rate and seed vigor index. The best breaking seed dormancy treatment was moist-chilling of 90 days for seeds of (Citruluus colocynthis) and gibberellic acid 800 ppm for (Silybum mrianum). The result showed that seeds of two species had the combination of physiological (related to seed inside inhibitors) and physical dormancy (related to hard coat).


A Study into the Effects of Salt Stress on Germination Components of Different Wheat (Triticum aestivum) Cultivars
Ali Namvar, Raouf Seyed Sharifi, Hashem Hadi,
Volume 4, Issue 2 ((Autumn & Winter) 2018)
Abstract

In order to study the effects of different levels of salinity on germination components of wheat cultivars, a factorial experiment was conducted based on a completely randomized design with three replications. Experimental factors were wheat cultivars at eight levels (Saysons, Gaspard, Finkan, MV-17, Chamran, Sardari, Atila-4 and Azar-2) and six levels of salinity (0, 25, 50, 100, 150 and 200 mM NaCl). The results indicated that salinity had significant effects on all of the traits studied (i.e., radicle length and dry weight, plumule length and dry weight, remaining seed weight after germination, seedling weight, the longest radicle and plumule, number of radicle per seed, ratio of radicle weight to plumule weight and rate, and uniformity and percentage of germination). All of these traits declined with an increase in the salinity (except the ratio of radicle weight to plumule weight, remaining seed weight after germination and seedling weight). The highest ratio of radicle weight to plumule weight, remaining seed weight after germination and seedling weight were recorded in the salinity of 200 mM NaCl. The cultivars showed significant effects on all of the traits studied (except remaining seed weight after germination and seedling weight). Chamran cultivar showed the highest number of radicle per seed, the ratio of radicle weight to plumule weight, rate and percentage of germination. The highest radicle length and the longest radicle were obtained from the Sardari cultivar while in Azar-2 cultivar it was vice versa in plumule. Atila-4 cultivar showed the highest radicle and plumule weight. It seems that compared with other cultivars, out of the cultivars studied, Chamran, Azar-2, Sardari and Atila-4 were more resistant to salinity stress.

 
 Highlights:

  1. Introduction of wheat cultivars with more tolerance to salinity conditions at germination stage.
  2. Study of eight different wheat cultivars in terms of germination indices under salinity stress conditions.

The Effect of Seed Inoculation with Organic and Biologic Fertilizers on Germination and Heterotrophic Seedling Indices in Rainfed Wheat (Triticum aestivum) Cultivar
Narjes Hojati Fahim, Mohamad Sedghi, Mehrdad Chaeichi, Rraouf Seyed Sharifi,
Volume 6, Issue 1 ((Spring and Summer) 2019)
Abstract


Extended Abstract
Introduction: Iran is located in the arid and semi-arid regions of the world with an average rainfall of 240 mm per year, which requires rethinking of the adoption of methods. One of the alternative ways is to use organic and biological fertilizers. Biological fertilizers are considered as the main and the most important factor in the integrated management of plant foods for sustainable agriculture as they play an important role in product improvement and efficiency. Therefore, this research was carried out with the aim of investigating the effect of seed pre-treatment with some organic and biological fertilizers in rainfed wheat.
Materials and Methods: A factorial experiment with three replications was conducted at the Laboratory of Seed and Plant Certification and Registration of the Center of Agriculture and Natural Resources Research located in Hamedan. Seed inoculation was considered in 5 levels (Seafull, Disper Root Gs., Bio-Health, Trichodermin and control) on 8 different rainfed wheat cultivars (Azar-2, Hashtrood, Baran, Rasad, Owhadi, Sardari, Takab and Homa). First, the seeds were disinfected with sodium hypochlorite, and were then cultured in special containers and were placed in the germinator at 20 ° C. After 4 and 8 days, the number of germinated seeds was counted. Germination seeds were counted in each treatment and germination indices such as germination rate, average daily germination, coefficient germination rate, longitudinal vigor index, weight vigor index, seedling fresh weight, root length/shoot ratio, shoot fresh weight, root fresh weight, seed reserve utilization rate, seed reserve utilization efficiency, fraction utilization seed reserve, seed dry weight were calculated.
Results: With application of different levels of fertilizer, the rate of germination treatment Homa×Bio-Health (155%), average daily germination treatment Hashtrood×Bio-Health (69%), coefficient germination rate treatment Owhadi×Disper Root Gs (60%), longitudinal vigor index treatment Owhadi×Bio-Health (108%), weight vigor index treatment Homa×Bio-Health (64%), root fresh weight treatment Hashtrood×Disper Root Gs (106%), shoot fresh weight treatment Hashtrood×Seafull (23%), seedling fresh weight treatment Homa×Bio-Health (42%), root length/shoot ratio treatment Owhadi×Trichodermin (75%), seed reserve utilization rate treatment Homa×Bio-Health (118%), and fraction utilization seed reserve treatment Homa×Bio-Health (119%) increased, compared with the control. In addition, the application of Bio-Health fertilizer and Hashtrood cultivar had the highest amount in almost all the mentioned attributes
Conclusion: Investigation of the different levels of fertilization showed that in most of the indices related to germination and heterotrophic growth of seedling, pretreatment with Bio-Health biofertilizer had a significant difference with other fertilizer levels.
 
 
Highlights:
  1. Evaluation of germination indices for recent cultivars of rainfed wheat, released by rainfed Research Institute of Iran.
  2. Investigating and comparing fertilizers with various multifactorial compounds (fungi and bacteria) and with each other.
  3. Conducting research on commercial compounds and comparison of live biochemical and non-organic matters in a single experiment.

The effect of priming on physiological and biochemical traits of French bean (Phaseolus vulgaris) under cobalt chloride stress
Khadijeh Sourazar, Mohammad Sedghi, Raouf Seyed Sharifi,
Volume 9, Issue 1 ((Spring and Summer) 2022)
Abstract
Extended Abstract
Introduction: The germination stage ensures the durability, establishment, and final yield of plants.  The final density of plants per unit area is resulted when the planted seeds germinate fully and with adequate rate. Plants mainly undergo abiotic stresses that are a considerable constraint for agricultural production worldwide. Seed priming is one of the simplest and cheap methods to improve seed germination, acceleration of seedling growth and establishment, uniformity, reduction of flowering time, vigorous seedling production, etc which leads to improved quality and yield of crop under stressful and non-stressful conditions. This study aimed to investigate the effect of different types of priming on enzymatic and physiological changes of French bean seed under cobalt chloride stress conditions.
Materials and Methods: To examine the effect of priming on germination indices, the activity of antioxidant enzymes and proteins in French bean under cobalt chloride stress, a factorial experiment was performed in a completely randomized design with three replications. These factors included four levels: seed priming with 100 mg / l salicylic acid and mannitol, control and distilled water (no priming), and three levels of cobalt chloride stress (0, 100, and 200 mg / l).
Results: The results showed that with increasing cobalt chloride concentration, germination indices (germination rate, root length, root fresh weight, stem fresh weight and stem dry weight) decreased. However, in the pretreatment of distilled water, salicylic acid, and mannitol there was a significant increase in germination indices (mean germination time, radicle length, plumule length, fresh radicle weight, and weight plumule) was observed. The interaction effect of priming and cobalt chloride caused an increase in radicle dry weight compared to control × stress treatment. The highest values of fresh radicle weight (14.4 g), dry weight of plumule (0.27 g), and plumule length (0.17 cm) were obtained in hydro priming pretreatment. The results also showed that hormone priming under stress conditions of 200 and 100 mg / l cobalt chloride increased protein content and polyphenol oxidase activity of French bean seedlings compared with the control treatment, respectively. Osmo priming increased the activity of catalase and peroxidase enzymes under stress and non-stress conditions resulted by 200 mg / l cobalt chloride.
Conclusion: according to the results of the present study, cobalt chloride reduced some physiological and biochemical traits of French bean seeds. However, the French bean has a relatively high cobalt chloride tolerance capacity, so that it showed good tolerance to different concentrations of cobalt chloride for up to 9 days.

Highlights:
1- The effect of different concentrations of cobalt chloride and different types of priming on the germination of French bean seeds was investigated.
2- French bean seedling protein content increased under the influence of hormone priming.

Effect of chitosan on germination indices of common bean (Phaseolus vulgaris) (cv. Sedri) seeds under salt stress
Haniyeh Saadat, Mohammad Sedghi, Raouf Seyed Sharifi, Salim Farzaneh,
Volume 9, Issue 2 ((Autumn & Winter) 2023)
Abstract

Introduction: Today, population growth has placed a significant burden on global agricultural resources. As a result, meeting global food demand and increasing farmers' incomes has become a challenging task. Salinity is one of the most harmful factors in the arid and semi-arid regions of the world that influences crop production. Seed priming is a technology by which seeds are physiologically and biochemically prepared for germination before being placed in their bed and exposed to the ecological conditions of the environment. The aim of this study was to evaluate the efficiency of the effect of chitosan on bean germination indices under salt stress.
Materials and Methods: The experiment was conducted as factorial based on a completely randomized design with four replications in a row (tube or sandwich culture) between filter paper at the University of Mohaghegh Ardabili in 2021. Treatments included four salinity levels (0, 50, 100, and 150 Mm) and four chitosan levels (0, 25, 50, and 75% by weight volume), all of which had been dissolved in 1% acetic acid. The studied traits included germination coefficient, allometric coefficient, daily germination rate, mean daily germination, seedling length and weight index, radicle length, plumule length, seedling length, radicle fresh and dry weight, plumule fresh and dry weight and residual dry weight. Data analysis was performed using the SAS 9.2 software and Duncan's test at p<0.05 probability level was used for mean comparison.
Results: The results showed that salinity stress decreased allometric coefficient (AC), seedling length vigor index (SLVI), radicle and seedling length (RL and SL), and radicle fresh and dry weight (RFW and RDW) and increased daily germination rate (DGS) and residual dry weight (RDW). Seed pretreatment with chitosan increased AC, SLVI, RL, SL, RFW, and RDW. The comparison of the means showed that there was a significant difference between the levels of chitosan so that the highest number of traits was obtained from the use of 75% chitosan and the lowest was obtained from the chitosan-free treatment. SLVI, SL, and RDW in 75% chitosan pre-treatment were higher at about 31, 26, and 27% compared to the control (priming with distilled water), respectively. The highest AC was observed in priming with 50% chitosan. Comparison of the mean for salinity stress also showed that the highest and lowest values of the measured traits respectively were obtained from the application of 0 and 150 Mm salinity levels. Also, with increasing salinity, chitosan increased germination coefficient (GC), seedling weight vigor index (SWVI), plumule length (PL), and plumule fresh and dry weight (PFW and PDW).
Conclusions: The results of this study show that among the different treatments, pre-treatment of seeds with 75 % Chitosan may be considered an effective way to improve seed germination of bean. It also can reduce the harmful effects of salinity stress on some traits in bean seedlings and improve seedling growth. Also, pretreatment with distilled water is an easy, low-cost and effective way to increase bean seedling germination and growth indices. Farmers can use this method for fast germination and better seedling growth under salt stress conditions.

Highlights:
  1. The effect of different concentrations of chitosan on the germination of bean seeds was investigated.
  2. Priming with chitosan increased the allometric coefficient, seedling length vigor index, radicle and seedling length, and radicle fresh and dry weight.
  3. A suitable method for reducing the harmful effects of salinity and increasing germination indicators of beans was introduced.

The effect of seed priming with Chitosan on the improvement of germination indices and biochemical characteristics of French Bean (Phaseolus vulgaris cv. Sadri) under salt stress
Haniyeh Saadat, Mohammad Sedghi, Raouf Seyed Sharifi, Salim Farzaneh,
Volume 10, Issue 2 ((Autumn & Winter) 2023)
Abstract
Extended Abstract
Introduction: At present, the increase in population has created an additional burden on the world's agricultural resources. As a result, meeting global food demand and increasing farmers' incomes has become a challenging task. Salinity is one of the abiotic stresses that strongly affects the germination, growth, and yield of crops. Seed priming is a simple technology that hydrates seeds to the point where the metabolic activity for germination is initiated, but no radicle emerges. In fact, the seeds are physiologically and biochemically ready to germinate before being placed in their bed and exposed to environmental conditions. This research was conducted with the aim of investigating the effect of seed priming with chitosan on germination indices and biochemical traits of beans under salt stress.
Materials and Methods: This experiment was conducted at the University of Mohaghegh Ardabili in 2021 as a factorial in the form of a completely randomized design with four repetitions. The treatments included four levels of salinity (0, 50, 100, and 150 mM) and four levels of chitosan (0, 0.25, 0.50, and 0.75% w/v), all of which were dissolved in 1% acetic acid.
Results: The results showed that salinity stress decreased germination percentage (GP), Peak value (PV), and germination value (GV). However, seed priming with different levels of chitosan, especially 0.75% chitosan, improved GP, PV, and GV by 7, 21, and 17%, respectively, compared to the control. With increasing salinity, the amount of proline and polyphenol oxidase enzyme activity increased, but the application of chitosan 0.75% increased these traits by 34% and 43%, respectively, compared to the control (priming with distilled water). Electrolyte leakage in priming with 0.75% chitosan decreased by 31% compared to the control, which indicates maintaining the stability of the cell membrane. The amount of soluble sugars in the treatment with 0.75% chitosan and 150 mM salinity showed an increase of about 78% compared to the control. The highest correlation among traits was observed between peak value and germination coefficient (0.995*) and between proline and polyphenol oxidase enzyme (0.917*).
Conclusions: The results of this study show that among the different treatments, pre-treatment of seeds with 75 % Chitosan may be considered as an effective way to improve germination indices and biochemical characteristics of bean and it can be used as a treatment to deal with salinity conditions in bean seedlings and improve the growth of seedlings.

Highlights:
  1. Seed priming using Chitosan improved Germination Indices of seed common bean under Salt Stress.
  2. Priming with chitosan increased Proline and Polyphenol Oxidase Enzyme.
  3. The concentration of 0.75 V/W of Chitosan revealed a better effect on germination indices and biochemical characteristics.
  4. Priming with chitosan can be a suitable method to reduce the negative effects of salinity, increase germination indicators, and improve the biochemical characteristics of beans.

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