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Mohsen Malek, Farshid Hassani, Enayat Rezvani Khorshidi, Ali Shayanfar, Bita Oskoee, Abbas Dehshiri,
Volume 9, Issue 1 ((Spring and Summer) 2022)
Abstract

Extended Abstract
Introduction: Galbanum is one of the most important native medicinal plants in Iran, and nowadays the use of its products and derivatives has become widespread in various industries. Thus, the excessive harvest of this species within its natural habitats has increased. Therefore, the species is enlisted as prone to extinction. Due to the lack of information about germination behavior and dormancy alleviating or breaking methods of Galbanum seeds, less attention has been paid to this plant species by seed science researchers. Hence, this study was designed and performed to investigate the effects of different treatments on Galbanum seed dormancy-breaking.
Materials and Methods: In this study, two Galbanum seed populations were collected from Boyer-Ahmad pastures (Iran, Kohgiluyeh and Boyer-Ahmad Province) and Pakan Bazr Isfahan Company (Iran). Seeds were exposed to a cold stratification test for 120 days and germinability and dormancy breaking responses were studied. Also, seed drying effects on germinability after exposure to stratification treatment were investigated to evaluate the practical aspects of this treatment. The impacts of different hormonal pre-treatments including gibberellic acid (GA3) and cytokines (Kinetin, 6-Benzylaminopurine) at different concentrations on germination and dormancy behaviors were investigated so as to assess the effect of these treatments on seed dormancy breaking.
Results: Results showed that germination/dormancy behaviors of Galbanum populations were different and also the effect of different treatments on seeds germination and dormancy varied. An increase in germination was observed when stratification was applied for up to 60 days on two seed populations, thereafter germination decreased by prolonged stratification. Also, it was revealed that desiccation of the treated seeds after stratification reduced seed germination. Examining the cause of this issue revealed that the most important reason for the decrease in seed germination in long periods of stratification was the germination of seeds in the stratification bed and in other words, the difference in seed dormancy levels in different stratification periods. Seed germination behavior and dormancy breaking were improved significantly in both Galbanum populations when the different concentration of gibberellic acid and cytokinins was used.
Conclusion: The results of this study indicated the existence of different levels of seed dormancy in different Galbanum populations. It was also found that cold stratification and hormonal pre-treatment of seeds can be significantly effective in seed dormancy breaking. Eventually, it was concluded that Galbanum seed dormancy was classified as semi-deep and deep morphological dormancy due to the effects of different treatments.

Highlights:
  1. The effect of cold stratification treatments over long periods was investigated on Galbanum seed germination behaviors for the first time.
  2. The desiccation of treated seeds under different cold stratification periods was studied on the germinability of Galbanum seeds for the first time.
  3. AUC (Area under the curve) index was introduced as a practical index in seed dormancy breaking comparison for the first time in domestic research.

Enayat Rezvani Khorshidi, Mohammad Reza Jazayeri, Leila Sadeghi, Mohammad Rahmani, Farshid Hasani, Bita Oskoee, Seied Hosein Jamali, Amirali Karimi,
Volume 10, Issue 1 ((Spring and Summer) 2023)
Abstract

Extended Abstract:
 Introduction: Production of high-quality seeds to stabilize crop yield is an important challenge for breeders. One of the most important answers to this challenge is to clarify the molecular mechanisms associated with seed vigor characteristics. Functional proteins of Cupin superfamily are among the molecules in signaling pathway. Previous research has shown that in maize, a storage protein similar to the functional Cupin superfamily protein called ZmGLP is effective in seed germination. However, in the previous experiments, suitable indicators were not used to assess seed vigor and its relationship with field establishment. So, it is needed to study the performance of ZmGLP in predicting field emergence to complete the previous research.
 Materials and Methods: An experiment was performed on 14 samples of commercial inbred maize lines. In this experiment, in addition to the laboratory evaluation of seed germination, field indices of physiological seed quality including the percentage of seedling emergence in the field, time to 50% seedling emergence, time to 90% seedling emergence, seedling dry weight, seedling height and coefficient of variation of seedling height was also assessed. In the polymerase chain reaction, two pairs of primers (CF / CR primers and IDF / IDR primers) were used to identify the DNA sequence of the Cupin.
Results: The results show that the seeds were different in terms of physiological quality. The lowest percentage of germination in laboratory was related to K1264/1, while the lowest physiological quality of seeds in field indices was observed in K1263/17. The molecular test confirmed the presence of the desired allele at the InDel9 site of vigor-related genes in the three samples of B73, K1264/1, and K1264/5-1, but no amplification band of the InDel9 site was observed in all K1263/17 seed samples. Due to the fact that line K1264/1, which had the lowest germination percentage in the laboratory, had an amplification band at this related site to vigor, it is not enough to rely on the results of the laboratory germination test to investigate the relationship between this gene and seed vigor. The field emergence test and seed vigor test that have a good prediction of field emergence must be used in these studies.
Conclusions: According to the results of this experiment, molecular tests with functional markers based on Indel9 can be used to accelerate the evaluation of vigor, especially when the breeder is breeding a new line or hybrid. It is a useful, rapid, and effective molecular method to predict seed emergence in the field and screen the lines to ensure the genetic strength of the germination of the lines, especially in the temperate germplasms of corn. Finally, it is necessary to determine the threshold of low vigor during seed quality investigation in different cultivars, and relationship between the presence or absence InDel9 site should be considered in future research.

Highlights:
1- The feasibility of using molecular markers to determine the seed vigor of corn lines in the field was studied and optimized for the first time.
2- The results of physiological quality assessment of seeds in the field for the studies related to the relationship between molecular markers and seed vigor were exploited for the first time.
3- The Indel9 site and molecular markers related to seed vigor in the field were introduced.


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