Extended Abstract
Introduction: Due to the fineness of parsley seeds, several problems may arise, such as the impossibility of using planting machines and the displacement of seeds by water, reduced germination and growth due to increased planting depth or lack of seed establishment in the soil, and consequently, increased seeding rate. Therefore, it is necessary to use methods to increase germination ability and improve the establishment of parsley seeds and seedlings in the soil. This experiment aimed to determine the most effective biopriming and gibberellin treatments for better germination and establishment of parsley seeds.
Materials and Methods: In order to determine the best biopriming and gibberellin priming treatments on germination characteristics and establishment of parsley seed, three experiments with four replications were conducted in the seed science and technology laboratory of Yasouj University in 2015 and 2016. The first biopriming experiment was carried out using growth-stimulating bacteria in a completely randomized design with eight treatments including bacterial isolates Pseudomonas fluorescens strain 21, Bacillus biosobetyl strain, Enterobactercus cloac strain 5, also two and three compounds of these bacteria along with control treatment. The second experiment was carried out with five treatments of Trichoderma harziarum (T36, T39, T42, and T43) isolates with control treatment. Finally, the third experiment was performed as a factorial in a completely randomized design with concentrations of gibberellin hormone (0, 50, 100, and 200 ppm) and prime times (6 and 12 hours). The measured traits were seedling length, seedling dry weight, germination percentage, and seedling length vigor index.
Results: The results showed that the best treatments for the first experiment were biopriming with Enterobacter + pseudomonas, for the second experiment biopriming with T36 fungus strain, and for the third experiment 50 ppm of gibberellin prime for 6 and 12 hours. The results showed that the majority of biopriming and hormone prime treatments improved the quality of parsley seeds so that the germination percentage in control seeds was 70%. This value increased by 31% compared to control treatment following priming with growth-stimulating bacteria (Pseudomonas+ Enterobacter), which showed the highest rate among all treatments applied in this study. The use of 50 ppm of gibberellin priming for 6 and 12 hours increased germination by 19% and 14% compared to the control treatment, respectively.
Conclusion: The results of this study showed that biopriming with Pseudomonas + enterobacter had the greatest effect on improving the quality and germination characteristics of parsley seed. In general, biopriming except for T42 fungi, and also gibberellin priming showed improvement in the quality and germination properties of parsley seed.
Highlights:
1. The effects of using biopriming and hormone prime are common, while it is not clear for parsley.
2. Biopriming with Pseudomonas+ enterobacter had the greatest effect on improving the quality and germination characteristics of parsley seeds.
3. Priming with T42 fungus reduced the quality and germination characteristics of parsley seeds.
