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Showing 37 results for Germination Rate

Fatemeh Lkhoshnoodashkarian, Marjan Diyanat, Gorban Noormohammadi,
Volume 7, Issue 1 (9-2020)
Abstract



Extended abstract
Introduction: London rocket is an important winter annual weed of the mustard family (Brassicaceae), which is propagated by seed. Germination of a seed population in response to water potential reduction is modeled using the concept of hydro time. This model has outputs that are physiologically and ecologically meaningful. One of the presumptions of the Hydro time model is the normal distribution of the base water potential among the seed population.
Materials and methods: In order to quantify the germination characteristics and determine the cardinal temperature of germination of London rocket (Sisymbrium irio L.), an experiment was done in 2018 at Science Research Branch, Islamic Azad University, Tehran, Iran. The seeds were placed at constant temperatures (5, 10, 15, 20, 25, 30, 35, 40 and 45 °C). Germination percentage, germination rate, root length, shoot length, seedling length and seedling fresh weight were evaluated. Intersected-lines, dent-like and quadratic polynomial models were used to determine cardinal temperatures. London rocket seed germination was tested across a range of water potential (0, -0.2, -0.4, -0.6 and -0.8 MPa) at the optimal temperature of 22.80 °C. The hydro time model, based on the normal distributions was fitted to data.
Results: Results showed that seed of London rocket did not germinate at temperatures of 5, 35, 40 and 45° C, and 25° C was the best temperature for seed germination (48%). The longest root length (4.49 mm) was observed at 20°C, which did not have significant differences with temperatures of 15 and 25 °C. The longest shoot length (10.19 mm) was obtained at 25 °C and there were not any significant differences among this temperature and temperatures of 15 and 20 °C. Similar trend with the trait of root length was observed for the trait of seedling length. The best model for estimating the cardinal temperatures in London rocket was intersected-line model with respect to coefficient of determination and mean square error. According to the intersected-lines model in London rocket, the minimum, optimum and maximum temperatures were calculated 5.83, 22.80 and 37.91°C. According to the hydro-time model based on normal distribution, the hydro-time constant and the base-water potential (which is a threshold for germination beginning) of London rocket degree were 284.28 (MPa/h) and -1.18 (MPa) at 22.80 °C, respectively.
Conclusions: Knowledge of germination and emergence of weeds also helps to predict the potential distribution to new habitats. The obtained coefficient of determination (0.94) between observed germination and predicted germination showed that the hydro time model based on normal distribution fitted well to germination percentage of London rocket seed. Due to the low hydrotime coefficient of this weed and the drought problem that most provinces face, it is expected that this weed will become more problematic in most provinces of Iran in the future.
 
Highlights:
1- The best temperature for germination of London rocket seed is 25 °C.
2- The best model for estimating the cardinal temperatures in London rocket is intersected-line model
3- The hydro-time constant and the base-water potential of London rocket degree based on normal distribution are 284.28 (MPa/h) and -1.18 (MPa) at 22.80 °C, respectively.

Seyyed Hamidreza Ramazani, Fariba Armoon, Mohammad Ali Behdani,
Volume 7, Issue 2 (3-2021)
Abstract

Extended Abstract
Introduction: Guar (Cyamopsis tetragonoloba L.) is a plant from the legumes family. Guar gum is obtained from endosperm in guar seeds. Guar gum is used in many industries such as pharmaceutical and food industries, paper, mining, oil and drilling, textiles, and explosives industries. Modeling is a method that is widely used in predicting plant growth stages and determining the required thermal units in each growing stage, especially germination.
Considering the important therapeutic and industrial uses of guar and the lack of sufficient information and reports to determine the cardinal temperatures of this plant, this study aimed to investigate the effect of temperature on germination traits and early seedling growth and predict the cardinal temperatures (minimum, optimal and maximum) of germination for this plant.
Materials and Methods: This research was carried out at the Seed Sciences and Technology Laboratory of Agricultural College of Sarayan, the University of Birjand in 2017. Experiments were carried out in a completely randomized design with 8 levels of temperature treatments (5, 10, 15, 20, 25, 30, 35, and 40°C), with 5 replications. Germination percentage, daily germination speed, mean daily germination, plumule length, root length, and seedling length were calculated. Cardinal temperatures of germination were calculated using regression analysis with the aid of the proposed models (logistic, two-way, quadratic, and third-order polynomials) using germination speed. The data were analyzed using SAS software and the comparison means were done by Duncan's test at a probability level of 5%. Sigma Plot software was used to plot the germination rate against temperature graphs (for fitting different models).
Results:  The results showed that the effect of different temperature levels on the percentage, speed and mean seed germination was significant (P <0.05). According to the results, the lowest values for percentage, speed, and average germination were obtained at 5, 10, and 40°C, and the highest germination speed was observed at 15 °C and also the highest percentage of germination and average germination was observed at 35°C. The results of the effect of different temperature levels on seedling growth showed that the effect of temperature on the seedling length, stem, and root length was significant (P <0.01), so that the lowest values related to seedling length, plumule, and radicle was found at 5, 10 and 40°C, and the maximum seedling and plumule length were 30°C.
Conclusion: Quantification of the gauge seed germination reaction to different temperature levels was carried out using four dual-functions, logistic, quadratic and triple polynomials. The second-order multitasking regression model, based on the coefficient of explanation (R2) and the amount of deviation, had a suitable and significant fit with the data related to germination rate against the independent temperature variable. Based on the parameters of the model, the optimum temperature was obtained at 26.05°C and the minimum and maximum temperature of guar germination were calculated to be 6.09 and 40°C.

Highlights:
  1. Cardinal temperatures of guar seed germination were predicted.
  2. Based on cardinal germination temperatures, the planting date of guar became predictable.

Ahmad Zare, Elham Elahifard, Zahra Asadinejad,
Volume 7, Issue 2 (3-2021)
Abstract

 
Extended Abstract
Introduction: Syrian mesquite is introduced as a weed in wheat and barley fields, saffron, cotton and vegetables, and in orchards. The spread of this weed in different climates raises the question of how much native plant conditions can affect germination characteristics. Therefore, the purpose of this study was to evaluate the effect of native plant conditions on germination characteristics in response to environmental stresses (temperature, salinity, and drought).
Materials and Methods: To investigate dormancy elimination and germination response thresholds of two Syrian mesquite ecotypes (Khuzestan and Fars) to environmental factors (temperature, salinity, and drought), four separate experiments were carried out as factorial based on completely block design with three replications at Agricultural Sciences and Natural Resources University of Khuzestan. Treatments included immersion the seeds with concentrated sulfuric acid (96%) (0, 10, 15, 20, 25, and 30 min), different temperature (0, 5, 10, 15, 20, 25, 30, 35, 40, and 45 °C), salinity levels (0, 100, 200, 300, 400, 500, and 600 mM), and different levels of drought stress (0, 0.2, 0.4, 0.6, 0.8, 1 and 1.2 MPa).
Results: The time required for immersion of seeds in sulfuric acid to dormancy breaking for two ecotypes was different. The estimated parameters indicated time of immersion in sulfuric acid to reach 50% germination in the Khuzestan ecotype (11.38 min) was longer than the Fars ecotype (8.10 min). The Khuzestan ecotype was also able to germinate (45%) at 40 ° C, whereas germination in the Fars ecotype was stopped at this temperature. Germination rate and cumulative germination percentage at temperatures below 25 ° C were higher in Fars ecotype than in the Khuzestan ecotype. The results of the salinity experiment showed that 50% reduction in seed vigor index, final germination percentage, and germination rate in the Fars ecotype were 167.48, 404.46, and 307.02 mM respectively and in the Khuzestan ecotype were 0.89. 229, 380.16, and 299.57 mM, respectively. For drought treatments, 50% reduction in final germination percentage, seed vigor index, and germination rate in Fars ecotype were -0.50, -0.38, and -0.39 MPa, respectively, and in Khuzestan ecotype were -0.46, -0.46, and -0.50 MPa, respectively.
Conclusion: Overall, the results showed that native plant climatic conditions (latitude and longitude, elevation, rainfall, and temperature) can affect the degree of seed dormancy, resistance to environmental stresses such as temperature, salinity, and drought.

 
Highlights:
1- Seed germination response thresholds of two ecotypes of Fars and Khuzestan for exposure to temperature and drought and salinity stresses were compared.

Mahdi Asadi, Majid Rahimizadeh,
Volume 8, Issue 1 (9-2021)
Abstract

Extended abstract
Introduction: Velvetleaf is one of the most important weeds of cotton, corn, tomato, and soybean fields. Certainly, knowledge of weed seed response to environmental factors (light and temperature) is essential for better understanding the germination mechanism and establishment patterns of weeds community. The present study aimed to evaluate the interaction between light regimes and alternate temperature on the seed germination of velvetleaf.
Materials and Methods: The experiment was conducted in 2015 at the plant physiology laboratory of Bojnourd Branch, IAU. This study was performed as a factorial experiment based on a completely randomized design (CRD) with four replications. The treatments were temperature regimes at four levels (constant temperatures 25°C, alternating temperatures 25-15, 30-20 and 35-25°C) and photoperiod treatments at three levels (continuous darkness, 12-12 light and dark and 16-8 light and dark). Germination percentage, germination rate, germination uniformity, time to 10% germination, and time to 90% germination were evaluated by the Germin program.
Results: The results showed that all traits were affected by temperature and light. Velvetleaf seeds germinated better in the presence of light and alternating temperature. The percentage and rate of germination increased as temperature rised to 30°c and then decreased. However, seed reaction to the night temperature was higher than that of the day temperature. The highest germination percent (98 percent) was achieved under alternating temperature 25-15°C with 12-12h light-dark. In this study, the lowest time required for 10% and 90% germination and highest germination uniformity were observed under alternating temperatures 30-20°C in darkness.
Conclusion: According to the results of this experiment, velvetleaf seeds are able to germinate in a wide range of light and temperature conditions, although they germinate better in the presence of light and alternate temperatures. Therefore, plowing with a moldboard plow can stimulate germination and drain the soil seed bank.

Highlights:
1- Since light stimulates the germination of velvetleaf seeds, so no-tillage system is able to control this weed.
2- Increasing the environment temperature reduces the chance of germination of velvetleaf seeds.             


Morteza Gorzin, Farshid Ghaderi-Far, Hamid Reza Sadeghipour, Ebrahim Zeinali,
Volume 8, Issue 1 (9-2021)
Abstract

Extended abstract
Introduction: Since the maximum percentage and rate of germination of rapeseed occur at a certain temperature, finding these temperatures can play an important role in determining the appropriate time and place for the cultivation of different cultivars. Also, light can affect the germination percentage of rapeseed at different temperatures, but the response of rapeseed to light, especially at lower and higher temperatures, has not been studied. Therefore, this study aimed to investigate the changes in the germination of rapeseed cultivars at different temperatures and determine cardinal germination temperatures based on germination percentage and rate under both the presence and absence of light conditions.
Materials and methods: In this study, germination tests were carried out at 5, 10, 15, 20, 25, 30, 35, 37, and 40°C temperatures in two light conditions (12 h light / 12 h dark) and darkness on nine spring cultivars (Traper, Agamax, Hayola-50, Hayola-420, RGS, Mahtab, Hayola-61, Zafar, and Zarfam) and one winter cultivar (Garo). The four-parameter Hill model was used to describe germination changes over time and the dent model was used to calculate cardinal temperatures. Seed viability at lower and higher temperatures was evaluated by the tetrazolium test.
Results: The evaluation of the trend of cumulative germination percentage over time in different cultivars showed that maximum germination percentage of all cultivars happened in the temperature range between 15-30 °C, some in the temperature range of 10-30 °C (Hyola-61) and others even in the temperature range of 5-30 °C (RGS, Mahtab, Garo, Zafar, and Zarfam) had the highest germination percentage. The highest germination rate in all cultivars was observed at the temperature range of 22-35 °C. Light only had an effect on the germination percentage of the seeds at sub and super optimal temperatures. At these temperatures, light increased the germination percentage. The remaining seed of 5, 10, 35, 37, and 40 °C temperature after transfer to 20 °C did not germinate, whereas most of them were viable based on the tetrazolium test.
Conclusion: The difference in the optimum temperature range for germination percentage and rate showed that to optimize seed performance, the optimal temperature range between the germination percentage and germination rate should be considered as the optimum temperature for germination. At sub and supra optimal temperatures, light leads to improved germination in some cultivars. The effect of light on germination at supra optimal temperatures was far higher than that of sub-optimal ones. Survival of the remaining seeds at the sub and supra optimal temperatures in some cultivars provided evidence of thermo-dormancy in these cultivars, this issue needs further investigation in the future.

Highlights:
1- The cardinal temperatures were studied based on both the percentage and rate of germination and the effect of light on them.
2- Some new varieties such as Traper and Agamax that little information about their characteristics is available were examined.
3- In this study, the reason for the lack of germination of rapeseed at the sub and supra optimal temperatures especially in the darkness has been mentioned.

Marjan Diyanat, Pouya Sonboli-Hamedani, Fereidoon Ghasem-Khan Ghajar,
Volume 8, Issue 1 (9-2021)
Abstract

Extended Abstract
Introduction: Seed dormancy is the most important factor that prevents weed seed germination. Lack of simultaneous germination of weed seeds causes a number of problems in their control due to seed dormancy in the fields; therefore, weed seed dormancy is considered to be an undesirable trait for farmers. The aim of this study was to investigate the effect of magnetic field on seed dormancy elimination in some weed species.
Material and Methods: In order to study the effect of Magnetic field on germination and seedling growth of redroot pigweed (Amaranthus retroflexus), spring wild oat (Avena fatua) and common purslane (Portulaca oleraceae), a factorial experiment based on completely randomized design was conducted at the Ecology laboratory of Science Research Branch, Islamic Azad University in 2019. Factors consisted of dormancy breaking treatment at 8 levels (gibberellic acid 1000 mg/l for 20 minutes, gibberellic acid 2000 mg/l for 20 minutes, sulfuric acid for 5 minutes, sulfuric acid for 10 minutes, sulfuric acid for 20 minutes, nitrate potassium at 0.01 g/L, nitrate potassium at 0.05 g/L and control) and magnetic field at 4 levels (0, 25, 50 and 100 mT).
Results: Results showed that responses of three weed species to magnetic field were different. Magnetic field increased percentage of germination, fresh weight of plumule and length of plumule of redroot pigweed, so magnetic field at 100 mT was the best treatment for dormancy breaking. The highest germination percentage of wild oat was obtained in 0.01 mM potassium nitrate at 50 mT. The length plumule of wild oat increased significantly at 2000 mg/l gibberellic acid with increasing magnetic field level from zero to 25 mM. Nitrate potassium at 0.05 g/L was the best treatment for breaking the dormancy of common purslane. Magnetic field at 100 mT could increase percentage of germination of this weed.
Conclusion: In general, the results of this study showed that sulfuric acid treatment was not a suitable option for the removal of dormancy in the three weed species because of the elimination of seed embryos. Magnetic field treatment significantly increased the studied traits in all three species of redroot pigweed, spring wild oat and purslane. The interaction between dormancy breaking treatment and magnetic field was significant in many of the studied traits and the effect of dormancy breaking treatment was intensified by magnetic field. Therefore, the use of magnetic field treatment is recommended to increase the germination of these three species.
 
Highlights:
1- There is a positive and significant interaction between seed dormancy elimination treatments and magnetic field.
2- Sulfuric acid treatment is not a suitable option for breaking the dormancy of redroot pigweed, spring wild oat and purslane.

Majid Ghanbari, Seyed Ali Mohammad Modarres-Sanavy, Ali Mokhtassi-Bidgoli,
Volume 8, Issue 1 (9-2021)
Abstract

Extended Abstract
Introduction: Medicinal herbs are of particular importance in the treatment and prevention of diseases. Indian Cheese Maker has strengthening, liver repair, anti-inflammatory properties and is useful in the treatment of bronchitis, asthma, wounds, neurological disorders such as Parkinson's and Alzheimer's. Evaluation of seed quality as a propagating organ and the most important input for crop production and medicinal products has a special place in seed production, control and certification. Studying germination and biological properties of seeds of medicinal plants and methods of breaking dormancy in them are among basic and primary studies of domestication of medicinal plants. In the meantime, scrubbing with abrasives changes the integrity of the seed shell and allows the seeds to be permeable to water and gases. The researchers stated that the dormancy of seeds containing inhibitory metabolic materials can be reduced by removing the seed shell through mechanical scarification and osmopriming. For this purpose, the effect of scarification and potassium nitrate on germination and enzymatic properties of Indian Cheese Maker was evaluated.
Material and Method: This study was conducted as factorial based on a completely randomized design with three replications during 2015-16 at the laboratory of Department of Agronomy, Tarbiat Modares University. Potassium nitrate solution (0, 0.5, 1 and 1.5 mg.l-1 from KNO3), scarification (un-use and scarification with soft sanding) and osmopriming durations (8, 16, 24 and 32 hour) were experimental factors. The experiment was performed on Indian Cheese Maker seeds, landrace of Khash. Petri dishes were placed in a germinator at 25 ° C and in full lighting for 14 days. In this experiment, germination rate and percentage of germination, mean of germination time and daily germination, seed vigority, alpha and beta amylase were measured.
Results: The results of the experiment showed that in scarification, the highest germination percentage (69.47%) was obtained by seed priming at a concentration of 1.5 mg.l-1 potassium nitrate for 19 hours under abrasion. In scarification, germination rate increased at 16 and 32 hour, 0.62 and 1.17 No.day-1 for each mg.l-1 of potassium nitrate. The highest daily mean germination (0.15) was observed at 1.5 mg.l-1 potassium nitrate and 24 hour time and decreased to 8 hours mean germination time (7.39 days) by reducing pretreatment time. Also, the highest mean germination time (9.35 days) was observed in 32 hours pretreatment with potassium nitrate and the highest mean germination time in non-scarification condition (9.13 days) and in scarification condition decreased with mean of germination time (8.04 days). The activity of alpha and beta-amylase germination enzymes was affected by different concentrations of potassium nitrate and scarification and at high concentrations of potassium nitrate the activity of these enzymes decreased.
Conclusions: In general, application of potassium nitrate osmopriming, by improving the activity of germination enzymes and increasing seed germination properties of Indian Cheese Maker, increased the activity of hydrolyzing enzymes in the endosperm of germinated seeds, which reduced the mean germination time, increased germination rate and germination percentage. In general, seed scarification with low concentrations of potassium nitrate at 16 to 24 hours is recommended for breaking seed dormancy of Indian Cheese Maker.

 
Highlights:
  1. Germination rate and percentage of Indian Cheese Maker seed were monitored by osmopriming and scarification.
  2. The role of α and β amylase germination enzymes in accelerating dormancy breaking of Indian Cheese Maker was studied.
  3. Mean time and mean daily germination during the dormancy breaking process of Indian Cheese Maker were estimated.

Kamran Alimardani, Amin Salehi, Mohsen Movahhedi Dehnavi, Ali Moradi,
Volume 8, Issue 2 (3-2022)
Abstract

Extended Abstract
Introduction: Schrophularia striata is one of the medicinal plants of the Scrophulariaceae family and contains phenolic compounds. Locals have traditionally used this herbal medicine to treat infections caused by wounds, gastrointestinal diseases, and eye diseases. Due to excessive consumption by indigenous people and climate change, especially increasing temperature, the cold required to eliminate seed dormancy is not presently available and its germination and growth has decreased. Therefore, this plant is at the risk of extinction. Since this plant is propagated in natural habitats through seed and due to deep seed dormancy, evaluation of different seed dormancy methods is necessary for conservation and domestication of this species. In this study, suitable methods to eliminate seed dormancy of this plant using chilling and gibberellic acid treatments were studied on the seeds collected from different habitats of Ilam province.
Materials and Methods: To investigate the effect of using chilling and gibberellic acid on Schrophularia striata dormancy, a split plot factorial experiment based on completely randomized design with four replications was conducted at the seed laboratory of Yasouj University in 2018. The first factor included the duration of moist chilling period (0, 4, 8, 12 and 16 weeks), the second factor included gibberellic acid (0, 300 and 600 mg/L) and the third factor included five habitats (Ilam, Ivan, Mehran, Abadan and Dehloran).
Results: The results showed that the 16-week Chilling and zero gibberellic acid treatments had the highest germination percentage in all habitats, as the percentage of germination in Mehran, Ivan, Ilam, Abadan and Dehloran habitats was 66, 50, 36, 30 and 25%, respectively. Also, the highest germination rate was observed in all habitats at 16 weeks chilling and zero mg/L gibberellic acid concentration. The highest germination uniformity was obtained at 16 and 12 weeks of chilling.
Conclusions: Chilling period duration was effective on germination percentage and rate and with increasing chilling period, germination percentage and rate increased. This indicates that the seeds studied had some degree of physiological dormancy, and the seed of higher altitude habitats required longer chilling periods than those of the lower ones for germination. Also, according to the results of this experiment, chilling periods above 16 weeks should be used to increase germination percentage.

Highlights:
1- Chilling period duration in the presence of gibberellic acid was the most important factor for the elimination of Schrophularia striata seed Dormancy and increased germination.
2- With increasing altitude and in the same chilling period duration, habitats with lower altitude had higher germination percentage and rate

Mohadeseh Latifzadeh Shahkhali, Seyed Mohmmad Reza Ehtehsami, Foad Moradi,
Volume 8, Issue 2 (3-2022)
Abstract

Extended abstract
Introduction: Seed deterioration is associated with oxidative stress and uncontrolled accumulation of reactive oxygen species. Seeds have a group of enzymatic and non-enzymatic antioxidants that protect them against reactive oxygen species and help maintain seed vigor and support seed germination processes. The response of different cultivars to seed deterioration is different. Knowledge about the sources and mechanisms of deterioration and how different cultivars respond can help to select the appropriate cultivar for the region and also provide useful information in selecting and applying appropriate management methods for storage and increasing the storage time of seeds.
Materials and Methods: The Experiment was conducted as factorial based on a completely randomized block design with three replications. Treatments included one and six months of natural storage (4˚C, 11 percent moisture content of seeds and 60% RH) and accelerated aging (96 hours at 45˚C and 100% RH) applied on four cultivars including Hashemi, Gilaneh, Khazar and Domsiyah. Germination percentage, germination rate, shoot length, radicle length, shoot dry weight, radicle dry weight, shoot vigor index (SVI), α-amylase activity, catalase (CAT), peroxidase (POX), superoxide dismutase (SOD) activity, malondialdehyde (MDA) and hydrogen peroxide (H2O2) content were evaluated.
Results: The results showed that accelerated ageing and storage of seeds for six months led to reduced germination percentage, germination rate, shoot length, radicle length, shoot dry weight, radicle dry weight, shoot vigor index (SVI), α-amylase activity reduced in all four cultivars. This decrease was accompanied by an increase in the free radical content of MDA and H2O2 and a decrease in the activity of the antioxidant enzymes SOD and POX. Malondialdehyde content was lower in the seeds stored for six months. The activity of CAT increased after deterioration treatment, this increase was more intense in the seeds stored for six months. These seeds also showed lower H2O2 content compared to accelerated aging seeds. Khazar cultivar showed lower H2O2 content as a result of higher CAT enzyme activity. Moreover, this cultivar showed better germination percentage and germination rate after deterioration treatment compared to other cultivars. The decrease in germination percentage due to deterioration in Domsiyah was very severe. This cultivar showed the lowest germination percentage, germination rate, seed vigor and α-amylase activity in the accelerated aging treatment. Gilaneh and Hashemi cultivars showed higher germinability, α-amylase activity and SOD and POX at the beginning of the experiment, but after deterioration treatment, their germinability decreased along with the activity of antioxidant enzymes.
Conclusions: In all studied cultivars, the accelerated aging and storage of seeds for six months adversely affected germination rate. This decrease was accompanied by an increase in free radicals in the seeds and a decrease in the activity of antioxidant enzymes SOD and POX, which were less severe in Khazar cultivar and more severe in Domsiyah cultivar. These results indicate the greater importance of the storage conditions in Domsiyah cultivar.

Highlights:
1- The effect of storage and seed deterioration on the activity of antioxidant enzymes, reactive oxygen species and seed germination in rice was investigated.
2- The results of accelerated aging test and natural aging were compared for a better conclusion about the response of the cultivars.
3- The two improved cultivars were compared with two local cultivars (with high cultivation area).

Meysam Miri, Mohammdreza Amerian, Mohsen Edalat, Mehdi Baradaran Firouzabadi, Hasan Makarian,
Volume 8, Issue 2 (3-2022)
Abstract

Extended Abstract
 Introduction: Germination is considered the first and most important stage of establishment and consequently, successful competition which is influenced by genetic and environmental factors. Among the environmental factors influencing the germination, temperature and light are the most important ones. Using different models, the germination response of seeds to temperature can be quantified; therefore, this study was performed to investigate the effect of temperature on germination and to quantify the germination response of Buckwheat seed (Fagopyrum esculentum Moenc) to temperature using nonlinear regression models and thermal-time model.
Materials and methods: The seeds were germinated in 4 replications of 25 seeds under 8 constant temperature treatments (5, 10, 15, 20, 25, 30, 35 and 40 ° C). Using a three-parameter logistic model, Buckwheat seed germination was quantified at different temperature levels and the percentage and time to reach 50% germination were obtained. Four nonlinear regression models and a thermal-time model were used to quantify the response of Buckwheat seed germination rate to temperature. To compare the models and determine the most appropriate model, the root mean square error index (RMSE), coefficient of determination (R2), coefficient of variation (CV) and standard error (SE) were used for the observed germination rate versus the predicted germination rate.
Results: The results indicated that temperature affected the seedling length, normal seedling percentage, seed vigor and the germination rate as well as germination percentage. Also, the results showed that germination characteristics increased with increasing temperature up to 20 and 25 °C. Comparison of the three models based on the root mean square error (RMSE) of germination time, the coefficient of determination (R2), CV and SE, the best model to determine the cardinal temperatures of Fagopyrum esculentum was the dent-like model. The results of thermal-time model showed that the base temperature of Fagopyrum esculentum seeds was 4.01 ° C and the thermal-time coefficient was 1242.6 h° C.
Conclusion: Utilization of non-linear regression models (segmented, dent-like and beta) and thermal-time model to quantify the germination response of Fagopyrum esculentum response to different temperatures led to acceptable results. Therefore, germination rate and percentage may be predicted using the outputs of these models at different temperatures.

Highlights:
  1. The best temperature for Fagopyrum esculentum Moenc. seed germination is 20-25 Celsius.
  2. The dent-like model was determined the most appropriate model for estimating the cardinal temperatures of Buckwheat.

Seyedeh Tina Mousavi Kani, Davoud Kartoolinejad, Zohreh Bahrami, Ali Asghar Zolfaghari, Elahe Nikouee,
Volume 9, Issue 1 (9-2022)
Abstract

Extended Abstract
Introduction: Nowadays, due to land use change and destruction of natural resources induced by human activities, attention to desertification has immensely become widespread. One of the most important species for biological rehabilitation of desert areas on a large scale is the hawthorn plant. Hawthorn is a plant belonging to the family Amaranthaceae, the two species of which are called white saxaul (Haloxylon persicum Bunge ex Boissier & Buhse) and black saxaul (Haloxylon aphyllum (Minkw.) Iljin) are more notorious and widespread in Iran. These plants own extensive root systems and stabilize running sands. The aim of this study was to investigate the effect of mesoporous titanium dioxide nanoparticles on germination traits of black saxaul seeds under drought stress, as an important species in desert areas.
Materials and Methods: The experiment was performed as a factorial experiment in a completely randomized design with 3 replications. Experimental treatments included five titanium nano dioxides with concentrations of 0, 25, 50, 100 and 200 mg / l and six drought stress levels including 0, -4, -8, -12, -16 and -20 bar. After nanopriming, black saxaul seeds were subjected to drought stress for 15 days in a germinator at a temperature of 20 °C with four replications. After germination, in each of the treatments, germination percentage (GP), germination rate (GS), mean germination time (MGT) and seed vigor index (VI) were calculated.
Results: The results of two-way ANOVA and mean comparison of germination indices showed that the highest germination percentage (74%) belonged to the zero level of stress treatment with a concentration of 200 mg/l and the lowest one (3%) belonged to -20 bar treatment with 25 and 200 mg/l nano-titanium dioxide. With increasing drought stress intensity from zero to -20 bar level, germination rate decreased from 7.01 to 0.43 seeds per day. The highest amount of seed vigor index at zero drought level belonged to the treatment of 200 mg/l concentration of nano-titanium, with a rate of 325.77.
Conclusion: The results showed that germination indices and initial growth of black saxaul seeds decreased in all primed and unprimed treatments with increasing drought stress, and nanopriming with titanium dioxide had a positive effect on seed germination traits.

Highlights:

1- Seed priming using mesoporous nano-titanium dioxide improved the germination percentage of black saxaul seeds under drought stress.
2- The concentration of 200 mg/l of mesoporous nano titanium dioxide revealed a better effect on seed germination index, mean germination time, germination rate and percentage
3- Seed nano priming using titanium dioxide did not have much effect on the growth characteristics of black saxaul seedlings at higher levels of drought stress.
 
Asieh Siahmarguee, Farshid Ghaderifa, Javid Gherekhloo, Atefeh Akbari Gelvardi, Maryam Gorgani,
Volume 9, Issue 1 (9-2022)
Abstract

Extended abstract:
Introduction: Reports indicate the invasion of two species of Ivy-leaved morning-glory (Ipomoea hederaceae Jacq) and Asian spider flower (Cleome viscosa L.) to summer crops fields such as soybeans in Golestan province. Considering the importance of knowing the cardinal temperatures of germination in the models for predicting the presence of weeds and consequently designing correct management strategies on these plants, this experiment was conducted to study the germination behavior and to compare the cardinal temperatures of germination for two species of Ivy-leaved morning-glory and Asian spider flower under two conditions of constant and alternating temperatures.
Materials and Methods: In order to study the effects of constant and alternating temperature on the percent, rate and estimating cardinal temperature of germination for Ivy-leaved morning glory and Asian spider flower, two separate experiments as a Completely Randomized Design with four replications were carried out at the seed laboratory of Gorgan University of Agricultural Sciences and Natural Resources. Studied temperature treatments on Ivy-leaved morning glory included the constant temperatures of 10,15,17,20,25,30,35 and 40 oC and alternating temperatures of 12.5:7.5,10:15, 12.5:20, 15:25, 20:30, 25:37.5, 30:42.5 and 35:45 oC. Temperature treatments on Asian spider flower included the constant temperatures of 15, 20, 23, 25, 28, 30, 35, 40, 45 and 50 o C and alternating temperatures of 15:20, 20:25, 20:30, 25:30, 25:35, 30:40, 30:45, 30:50 and 40:50 oC.
Results: Results indicated that alternating temperatures had a positive effect on the germination of Ivy-leaved morning glory, which increased from 78% at constant temperatures to 94% at alternative temperatures. However, the maximum germination percentage of Asian spiderflower at the alternative temperature of 20:30 was 79%, which had no significant difference with a constant temperature of 30 with 84% germination. The base temperature for seed germination of Ivy-leaved morning glory at alternating temperatures (10.09 oC) was a little lower than that of constant temperatures (11.25 oC). But in Asian spider flower, the base temperature at alternating temperatures (17.57 oC) was more than that of constant temperatures (15.43 o C).
Conclusion: The most important factor in the occurrence of such different responses to constant and alternating temperatures in Ivy-leaved morning glory and Asian spider flower is their adaptation to environmental conditions and their survival in highly degraded agricultural environments. These two weeds are warm-season plants and their seeds enter the soil seed bank in autumn. The presence of dormancy (regardless of its type) in the seeds of these two plants prevents the germination of their seeds in winter; and the experience of alternating temperatures at this time prepares the seeds for germination in the spring. The base temperature of Ivy-leaved morning glory was not much different under the constant and alternating temperature conditions. But the base temperature of Asian spider flower was higher under alternating temperatures compared with constant temperatures.
 
Highlights:
1- The Response of germination percentage of Ivy-leaved morning-glory and Asian spider flower were investigated under constant and alternating temperatures.
2- Cardinal temperatures of germination for these two weeds were compared at constant and alternating temperature conditions.

Emad Shahmansouri, Zahra Abbasi,
Volume 10, Issue 1 (9-2023)
Abstract

Extended Abstract
Introduction: The selection of optimum planting date for every climate and mother bulb size are important factors affecting the quantity and quality of seed production in different cultivars. This study was performed in Isfahan province, Frieden, during two growing years 2016-2017 to determine the effect of planting time and mother bulb size on the quantity and quality of short-day onion seed varieties Texas Early Grano 502
Materials and Methods: A split-plot experiment was performed in a randomized complete block design with four replications. The main factor was cultivation dates at four levels: the end of August, the first of September, the end of September and the first of October and mother bulb size at three levels with 3.55, 5.5-7, and 7.5-9cm diameters were assigned as sub-plots. The measured traits were number of umbel per unit area, the number of fertilized capsules in umbel, the number of seeds per capsule, 1000 seed weight, seed yield and germination rate.
Results: The results of combined analysis of variance showed that effect of year was only significant on the number of capsules per umbel at p<0.01. The interactions of year× mother bulb diameter and year× planting date× mother bulb diameter were not significant for any of the traits. The traits were not affected by the year. The results showed that the planting date had a significant effect on all traits except for 1000 seed weight, and the first planting date (the end of August) had the highest values in all traits. The highest germination percentage (89.83%) and the highest germination rate (12.8) were obtained on the first planting date, which showed a statistically significant difference with the subsequent planting dates. However, these two traits were not affected by mother bulb diameter treatment. The highest seed yield for onion diameter factor (505.65 kg h-1in the first year and 465.78 kg h-1 in the second year) was associated with mother bulb size of 7.5-9 cm.
Conclusions: The overall result showed that to obtain the highest and most desirable quality of onion seeds of Texas Early Grano 502 cultivar in Friedan of Isfahan, the planting date of the first of September and the size of the mother bulb between” 7 to 9 cm is recommended.

Highlights:
1- Early planting time (the first of September) increased germination rate.
2- Increasing the mother bulb size increased the onion yield.

Fatemeh Ghorbannezhad, Mohsen Zavareh, Farzad Sharifzadeh,
Volume 10, Issue 1 (9-2023)
Abstract

Extended abstract
Introduction: Linseed (Linum usitatissimum L.) is a multipurpose crop and is cultivated to obtain oil, fiber, and seeds. Under optimal moisture conditions, the temperature is considered an environmental factor affecting the germination of this crop. Hence, knowing the cardinal temperatures can help farmers to predict the successful germination, emergence, and even yield of linseed and help scientists to develop new cultivars that are more tolerant to high temperatures. Therefore, this study was performed to determine the temperature range and the cardinal temperatures of germination in two linseed genotypes.
Material and methods: The germination response of two linseed genotypes (Golchin genotype and Line 286) to nine temperatures (3, 5, 10, 15, 20, 25, 30, 35, and 40 Celsius degrees) was quantified in a CRD based split-plot experiment with four replications. For this purpose, three nonlinear regression models (beta, segmented, and dent-like) were used to fit to the data and select the superior model. The superior model was selected using the Akaike information index (AIC), the modified Akaike index (AICc), and ∆i.
Results: Findings showed that the beta model had the best performance in estimating the line 286 cardinal temperatures according to its lower AIC (-3.96), AICc (-89.61), and ∆i (0). Accordingly, the base, optimum, and maximum temperature as well as the number of biological hours estimated by this model for Line 286 were 7.18, 24.22, 40.16 Celsius degrees, and 19.25 hours, respectively. In the Golchin genotype, the beta model with the lowest AIC=-3.89 and AICc= -89.083 fitted better compared with the other models. Nonetheless, considering ∆i for beta which was respectively 0, 1.61, and 4.49 for beta, segmented, and dent-like models, Beta and segmented models had a similar accuracy in estimation of cardinal temperatures for Golchin genotype. These findings represent that the suitable temperature range for germination of the Golchin genotype is 3.8- 23.85 Celsius degrees and the range of biological hours to 50% of germination varied from 16.42 to 19.77 hours.
Conclusion: Overall, according to the results of this study, it is possible to predict the time to germination under optimal moisture conditions using the beta model for Line 286 and one of the two beta and segmented models for the Golchin genotype.

Highlights:
1. A suitable model was developed for a suitable prediction of the seed germination percentage of two linseed genotypes (Golchin genotype and Line 286).
2. The cardinal temperatures for two linseed genotypes (Golchin genotype and Line 286) were determined.

Mahnaz Kandi, Asa Ebrahimi, Marjan Diyanat, Hamid Saeidian,
Volume 10, Issue 1 (9-2023)
Abstract

Extended Abstract
Introduction: Weeds are one of the most important problems in agriculture and cause a lot of damage to crops. Overconsumption of herbicides in recent decades has led to harmful impacts on the environment and living organisms, including humans. In order to deal with these problems today, efforts are being made to reduce reliance on artificial herbicides and change direction towards sustainable agriculture as a part of integrated weed management. Among natural compounds, essential oils, which are components of aromatic and medicinal plants, were investigated to discover new herbicides.
Material and Methods: In order to investigate the effects of yarrow essential oil (Achillea wilhelmsii C. Koch) on the germination and growth of purslane (Portulaca oleracea L.) and rye (Secale cereale L.) seedlings, two factorial experiments were conducted in the form of a completely randomized design in three replicates at Islamic Azad University, Science and Research Branch. The first factor was the type of essential oil in two levels (normal and nanoemulsion) and the second factor was the concentration of essential oil in six levels (0, 100, 200, 400, 800, and 1000 µl L-1).
Results and Discussion: Scanning electron microscopy images showed the particles were relatively sperhical and their size varied from 1 to 100 nm. The zeta potential of the nanoparticles was -13.8 mV, indicating particle stability. Both type of essential oils reduced germination percentage, germination rate, plumule length, radicel length and seedling dry weight of both weeds and germination percentage decreased significantly with increasing concentration. At a concentration of 200 µl L-1, purslane treated with essential oil germinated 55.25%, but at the same concentration, the germination percentage of seeds treated with nanoemulsion of essential oil was 30.50%. Nanoemulsion of essential oil at concentration of 800 µl L-1 was able to completely prevent the germination and growth of purslane seedlings. Nanoemulsion of essential oil at a concentration of 1000 µl L-1 completely prevented rye seed germination. Germination rate decreased under treatment of yarrow essential oil and there was no difference between two types of essential oils at concentration of 200 µl L-1, but at a concentration of 800 µl, the germination rate of rye seeds treated with nanoemulsion of essential oil and ordinary essential oil reached to 1.40 and 0.32, respectively which was a significant difference.
Conclusions: The inhibition percentage of the studied traits in both weed species showed that the essential oil nanoemulsion was more effective than the normal essential oil. Yarrow essential oil was more effective on purslane weed compared to rye. Essential oils do not have similar effects on weeds and their inhibitory effects will be different depending on the concentration and species of weeds, and the selectivity of these compounds should be investigated. The production of nano essential oil of yarrow as a natural herbicide is a solution. It offers to control weeds in organic farming systems.

Highlights:
1- Nanoemulsion particles were produced from Yarrow essential oil
 2- Nanoemulsion particles had more inhibitory effects on the investigated weeds than the normal essential oil.

Ahmad Munir Amini, Farshid Ghaderi-Far, Dr Benjamin Torabi, Asieh Siahmargue, Hamid Reza Sadeghipour,
Volume 10, Issue 2 (3-2024)
Abstract

Extended abstract
Introduction: With regard to the ever-growing water deficit in the world, the adoption of the direct-seeded rice cultivation system has been suggested as an alternative to the transplanting method. One of the disadvantages of the direct-seeded method is low and non-uniform germination and emergence due to low seed vigor in rice. Priming is a technique which improves the rate and uniformity of seed germination under these conditions. Thus, this study aimed to investigate the effects of priming treatments on seed germination of different rice cultivars under different temperature conditions using the thermal time model.
Materials and methods: This study was conducted in 2019 at the seed research laboratory of Gorgan University of Agricultural Sciences and Natural Resources. In this experiment, germination of primed and non-primed seeds in three rice cultivars (Nada, Anam, and Tolo) was investigated under different temperatures (15, 20, 25, 30, and 35°C). The priming treatments which consisted of control, hydropriming, and osmopriming with different chemicals (potassium chloride 2%, potassium nitrate 1%, calcium chloride 4%, glycine betaine 10 ppm, salicylic acid 10 ppm, and ascorbic acid 10 ppm) were investigated under different temperatures.
Results: The results showed that priming treatments had no significant effect on the seed germination percentage of rice cultivars at different temperatures. The thermal time model based on binomial distribution fitted well to cumulative germination percentages in all priming treatments. Among the parameters of the thermal time model, the greatest priming effect was on the reduction of the thermal coefficient, followed by the reduction of the sigma coefficient, which resulted in the increased rate and uniformity of germination. Priming treatments had no significant effect on base temperature. Also, the responses of rice cultivars to seed priming treatments varied so that in Anam and Neda, priming with calcium chloride but in Tolo, hydropriming was more effective on the model parameters, especially thermal time to 50% of germination.
Conclusion: In general, priming treatments did not affect the base temperature of germination in rice cultivars, but they significantly affected the rate and uniformity of seed germination. As the latter issue is one of the main problems in the direct-seeded rice system, suitable priming treatments for each cultivar can be adopted to increase the rate and uniformity of seed germination and emergence in this system.

Highlights:
  1. The thermal time model can be used to select the appropriate priming treatment for improving seed germination components of rice cultivars.
  2. The responses of rice cultivars to different seed priming treatments were different.
  3. Priming treatments did not improve the base temperature of seed germination in rice cultivars, but they significantly affected seed germination rate and uniformity.

Mahboubeh Shahbazi, Jafar Asghari, Behnam Kamkar, Edris Taghvaie Salimi,
Volume 10, Issue 2 (3-2024)
Abstract

Extended abstract
Introduction: The germination process is one of the most critical stages of a plant's growth and determines the success of the emergence of a weed in an agroecosystem because it is the first stage in which the weed competes for a niche. Various environmental factors, including temperature and moisture, affect the germination of weed seeds. Modeling techniques are capable of predicting germination, seedling emergence, and establishment of weed species. The ability to predict weed germination in response to environmental conditions is very effective for the development of control programs. The experiment was conducted to determine the cardinal temperature and evaluate the best model for quantifying the response of the germination rate of Western ragweed weed seeds under different water stress conditions.
Materials and Methods: A factorial experiment was conducted in the form of a completely randomized design in three replications. The investigated factors include temperature with eight levels (5, 10, 15, 20, 25, 30, 35, and 40 C˚) and water potential with six levels (0, -0.3, -0.6, -0.9, -1.2, and -1.5 MPa) on the germination of Western ragweed. In order to quantify the response of Western ragweed germination rate to temperature, three non-linear Dent-like, Beta, and Segmented regression models were used.
Results: The results showed that the effect of temperature, water potential, and their interactions on maximum germination, germination rate, and time required to reach 10, 50, and 90 percent germination were significant. Also, the results showed that by increasing the temperature from 10 to 25 C˚, the percentage and rate of germination increased whereas by increasing water potential, the percentage and rate of germination decreased. In comparing the models, based on RMSE, R2, CV, and coefficients a and b parameters, the Beta model was the most suitable for estimating the temperatures of cardinal Western ragweed. The base, optimum, and ceiling temperatures using the Beta model were 3.88, 25, and 40 C˚, respectively.
Conclusions: The use of the Beta model to quantify the germination response of Western ragweed seeds to different levels of water potential at different temperatures had acceptable results. Therefore, by using the output of these models at different temperatures, it is possible to predict the germination rate at different potentials.

Highlights:
1- Germination cardinal temperatures and the effect of water potential on western ragweed weed were investigated.
2- Estimation of different models to quantify the response of germination rate to temperature and different water potentials.


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