Extended abstract
Introduction: Salvia sp. from Lamiaceae family is one of the important medicinal and pasture plants that have many uses in various medicinal and food industrial. This study was performed to evaluate the effects of different hormonal treatments on breaking seed dormancy in three species of Salvia including as S.officinalis, S.leriifolia, and S.syriaca under different wet stratification persiods.
Materials and Methods: This research was conducted in a split plot layout experiment in a completely randomized design (CRD) with 3 replications and 2 separate repetitions in Herbal Research Institute, Islamic Azad University Branch of Shahrekord in 2024. Experimental treatments included wet chilling (0, 10, 20, 30 day in 4°C and RH=45%) in main plots and hormones inclusive (gibberellic acid (120ppm), benzyl adenine (10-4 M), kinetin (10-4 M), gibberellic acid (120ppm)+ benzyl adenine (10-4 M), gibberellic acid (120ppm)+kinetin (10-4 M), benzyl adenine (10-4 M)+kinetin (10-4 M), gibberellic acid (120ppm)+benzyl adenine (10-4 M)+ kinetin (10-4 M) and control) in sub-plots. Biochemical indices such as hydrogen peroxide, α-amylase and beta-1,3glucanase and germination indices such as dry weight of seedlings, length of seedlings, germination percentage, mean of germination time, seed vigour and germination uniformity were measured.
Results: Use of wet chilling and hormones treatments, had significant effectiveness on germination characters. Gibberellic acid in the most of measured characters had beneficial and increasing effects. The most and lowest important measured germination charastreristics and decreased time needed for germination. The highest and lowest amounts for germination characteristics such as seedling length (5.56-2.1 cm), seed vigor (5.1-0.94), seedling dry weight (45.35-19.84 mg), germination percentage (91.74-38.22), germination uniformity (21.03-3.57) and biochemical characters such as content of alpha-amylase (12.94-2.6 mM/gr fresh weight of seed), beta-1, 3 glucanase (10.79-2.83 mM/gr fresh weight of seed) and hydrogen peroxide (0.69-0.21 mM/gr fresh weight of seed) were recorded under wet chilling treatment for 20 day+gibberellic acid (120ppm)+benzyl adenine(10-4M)+ kinetin (10-4M) and treatment of without wet chilling priming+without hormonal, respectively. Also increaring wet chilling treatment duration, increased some evaluated characteristics, but most of them reached highest values after 20 days wet chilling treatment. The amounts of evaluated characters in this treatment improved more than 100% in comparison to control seeds. It can be concluded that wet chilling (20 day) along with use of gibberellic acid, benzyl adenine and kinetin resulted in improved seed germination indices of Salvia sp. Results showed that in most of measured characteristics the Salvia species have various amounts of biochemical and germination indices. Finally the treatment of wet chilling (20 day) and use of gibberellic acid, benzyl adenine and kinetin hormones, suggest to obtain of the best germination indices in seeds of Salvia spp. Germination percentage and the most of another measured characters in this research in specie of officinalis was more than specie of syriaca was more than specie of leriifolia.
Conclusion: Results showed that in most of measured characters the Salvia species have various amounts of biochemical and germination indices. Generally wet chilling (20 day) with use of gibberellic acid, benzyl adenine and kinetin suggest to best seed germination of Salvia spp.
Highlights:
- Study the various primings of wet chilling and hormonal treatments on biochemical and germination indices of Salvia officinalis, S.leriifolia, S.syriaca
- Wet chilling (20 day) with use of gibberellic acid, benzyl adenine and kinetin suggest to best seed germination of Salvia spp.
