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Showing 11 results for Balouchi
The reaction of different cultivars of rice (Oryza sativa) to high and low salinity in vitro condition
Asad Masoumi Asl, Rreza Amiri Fahliani, Hamidreza Balouchi,
Volume 1, Issue 1 ((Spring and Summer) 2014)
Abstract
Salinity is one of the most extensive environmental stresses and is a limiting factor for the growth and development of crops, such as rice. Then, an experiment based upon completely randomized design with four low levels (0, 7.5, 15, 22.5) and four high of salinity (75, 150, 225 and 300 mM) with control as the first factor and nine cultivars of rice (dollars, Hasan Saraii, Mousa Tarom, Kamfiruz, Lenjan Askari, Gharib, Domsiah Mamassani, 304, Champa Yasouj) as the second factor with three replications was conducted in 2012 in the Yasouj University. Results showed that Hasan Saraii had salinity tolerance with the most germination percentage, germination rate and shoot length at high salinity levels (more than 75 mM), and Domsiah Mamassani with the minimum of that traits had not any salinity tolerance. But in the low salinity (less than 22.5 mM), Hasan Saraii, Lenjan and Mousa Tarom had the highest percentage of germination. The maximum germination rate was due to Mousa Tarom and the maximum shoot length observed in Gharib variety. So it seems, if we would only consider the germination percentage, Hasan Saraii is the best variety and if the maximum germination percentage and rate are considered, the variety that would be considered is Mousa Tarom. Therefore, high salinity we should not just rely on the germination but the germination rate should also be noted and in lower salinity due to different cultivars germination percentage, germination rate and dry weight of root should be paid much more attention.

Effect of Seed Priming on Some Germination Traits and Seedling Growth of Safflower under Drought Stress
Mozhgan Maleki Narg Mousa, Hamidreza Balouchi, Mahmood Attarzadeh,
Volume 2, Issue 1 ((Spring and Summer) 2015)
Abstract

In order to evaluate the effect of priming on some germination and seedling growth characteristics of safflower (Carthamus tinctorius L.) under drought stress, an experiment was conducted base on completely randomized design with four replications at the Seed Technology Laboratory of Yasouj University, in 2011. Treatments were included of five priming levels (control or no prime, hydro priming, potassium nitrate 3% (KNO3), PEG and Urea -4 bar) as the first factor and three levels of drought stress (0, -4 and -8 bar) as the second factor. The results showed that the interactions of drought stress and seed priming on germination percentage and the germination rate was not significant. But the main impact of priming and drought stress on them was so significant at 1% probability. The highest germination percentage and germination rate (75.66% and 3.62 seed.day-1, respectively) were shown in urea priming and the lowest (52% and 2.31 seed.day-1 respectively) in the control treatment (no prime). Drought stress was caused a significant decrease in germination traits. In general, the results obtained in this study showed that treatment with urea and potassium nitrate compared with PEG improved germination and seedling growth of safflower.


Allelopathic Effect of Yarrow (Achilla wilhelmsii) and Habek Mint (Mentha longifolia) on Seed Germination Indices of Plantain (Plantago major)
Yaghoub Behzadi, Amin Salehi, Hamidreza Balouchi, Fahimeh Khaledi,
Volume 2, Issue 2 ((Autumn & Winter) 2016)
Abstract

In recent years the use of allopathic effects on weed management seems to have attracted many professionals. In order to investigate the effect of aqueous extracts of Yarrow (Achilla wilhelmsii L.) and Habek mint (Mentha longifolia (L.) Huds) medicinal plant on germination indices of plantain, two experiments were conducted in completely randomized design with three replications in 2014 in the laboratory of seed technology of Yasouj university. Treatments consisted of 0, 25, 50, 75 and 100% concentrations of aqueous extract of Yarrow and Habek mint aerial parts. The results showed that the extract of Yarrow and Habek mint aerial parts on germination percentage, root and shoot length, root and shoot fresh and dry weight and seed vigor was significant. The maximum percentage and rate of germination have belonged to the control treatment (distilled water) and by decreasing the aqueous extract concentrations of each plant, the percentage and rate of germination increased. The minimum percentage and rate of germination have belonged to 100% concentration of aqueous extract that had no significant difference by 75% concentration. Finally, these results showed allelopathic effects of aqueous extract aerial parts of Yarrow and Habek mint, as a bio-herbicide on growth and germination of plantain.


Allelopathic Effect of Different Concentrations of Aqueous Extracts of Nine Weeds Species on Seed Germination and Seedling Characteristics of Anise (Pimpinella anisum)
Zahra Ajribzadeh, Hamidreza Balouchi, Alireza Yadavi, Amin Salehi,
Volume 4, Issue 1 ((Spring and Summer) 2017)
Abstract
In order to evaluate the allelopathic effect of aqueous extract of nine weeds species on Anise (Pimpinella anisum) seed germination and seedling growth characteristics, an experiment was conducted in the Laboratory of Yasouj University in 2014. This experiment was carried out as factorial with the aqueous extracts of nine weeds species, as the first factor, in different concentrations (10, 20, 30, 40 and 50 g.l-1), as the second factor, in a completely randomized design with four replications. The results showed that interactions between different weed aqueous extracts and concentrations had a significant impact on the germination percentage and rate, root and shoot length and weight, and vigor of anise seeds at 1% probability. The most allelopathic effects on germination percentage and rate were observed in 10 g/l of the aqueous extract of Cowherb and Corn Buttercup, in 20 to 30 g/l of Corn Buttercup and Whitetop, and in 40 to 50 g.l-1 of Corn Buttercup, Whitetop, Wild lettuce and Cowherb, which completely stopped seed germination in Anise.

Highlights:
  1.  Allelopathic effects of 9 common weedy types on germination of Anise Seed were studied in Kohgiluyeh and Boyerahmad.
  2. The effect of different concentrations of aqueous extracts of weeds on germination characteristics of anise was studied.

Effect of Zinc and Boron Foliar Application on Maternal Plant and Seed Enrichment on Vigor and Germination Indices of Sesame (Sesamum indicum cv. Darab 1)
Samira Parsaei, Mohsen Movahhedi Dehnavi, Hamidreza Balouchi,
Volume 4, Issue 1 ((Spring and Summer) 2017)
Abstract
Micronutrients play a great role in the production of high quality seeds, especially in terms of germination. Therefore, providing seeds with sufficient amounts of these is an important consideration in seed production. The present study was conducted as two separate experiments in the Seed Laboratory of Yasouj University in 2015. In the first experiment, the sesame seeds (cv. Darab 1) obtained from a previous split plot experiment based on RCBD, consisting of three irrigation levels (after 75, 110 and 145 mm evaporation from class A pan) and four foliar applications comprising water, zinc sulfate, boric acid and mixture of zinc sulfate and boric acid, were investigated in germination experiments in the minimum temperature (i.e., 15°C). The second experiment was a factorial based on RCD with three replications. The first factor in the eight levels included seeds soaked in water, ZnSo4, H3Bo3 and ZnSo4 + H3Bo3 and seeds with high contents of Zn (159 m Kg-1), B (15.3 m Kg-1) and Zn (139.5 m Kg-1)+ B (14 m Kg-1) and dry see as the control. The second factor was accelerated aging periods at three levels (0, 2 and 4 days) at 45°c and RH = 100%. The results showed that with a delay in irrigation time, compared with water foliar application, foliar application of micronutrients (Zn, B, Zn+B), and significantly increased germination traits. The results also suggested that, compared with seeds soaked in nutrients, seeds with high Zn and B contents significantly increased germination indices and antioxidant (catalase and peroxidase) activities, especially under accelerated seed aging.

Highlights:
  1.  The role of maternal sesame plant (cv. Darab 1) foliar application by zinc and boron elements in germination, vigour and storage capability of seeds was studied.
  2. The effect of seeds soaked in solutions enriched with zinc and boron elements on germination, vigour and storage capability of seeds was examined.

Effect of Seed Aging and Water Potential on Seed Germination and Biochemical Indices of Fenugreek (Trigonella foenum-graecum) at Different Temperatures
Hasan Teimori, Hamidreza Balouchi, Ali Moradi, Elias Soltani,
Volume 5, Issue 2 ((Autumn & Winter) 2019)
Abstract


Extended abstract
Introduction: Seed germination is one of the first important and complex stages in the plant life cycle and is affected by many hereditary and environmental factors. Various factors affect germination and seedling establishment. Among these factors are the characteristics of the maternal plant (nutrition, genetics), seed treatment stage at harvest time, as well as environmental factors (temperature, water potential, and ventilation and soil compaction). Also, under the influence of seed loss during storage, seed vigor, which is known as the first component of seed quality, decrease. The aim of this study was investigation of germination and biochemichal responses of the aged seed of Fenugreek to different temperature and humidity ranges.
Materials and Methods: This experiment was conducted as a factorial based on a completely randomized design with four replications in the Laboratory of Seed Science and Technology, Faculty of Agriculture, Yasouj University in 2016. The experimental treatments consisted of nine levels of temperature (5, 10, 15, 20, 25, 30, 35, 40 and 45 degrees Celsius), water potential included seven levels (zero (control), -0.2, -0.4, -0.6, -0.8, -1 and -1.2 MPa) and seed aging at two levels (no aged (control) and aged seed).
Results: In this experiment, the effect of seed aging, water potential and their interactions on each environment on germination indices (germination percentage and germination rate, length and weight vigor index) and biochemical indices (soluble sugar, proline, soluble protein and catalase enzymes) of Fenugreek seeds were significant. The results showed that in the aged seed the germination percentage and rate and seedling vigor index tended to decrease with water potential reduce in temperature lower and higher than 20 degrees Celsius, and the amount of biochemical components of the seed (soluble sugar, soluble protein, proline, and catalase enzyme) also increase.
Conclusion: In general, germination and biochemical indices of seed of Fenugreek are sensitive to water potentials, aging, and seed germination temperatures, respectively. In terms of osmotic potential decrese, the germination temperature of less than 20 ° C resulted in increased germination resistance of fenugreek seed to a more negative water potential.
 
Highlights:
  1. Study of germination and biochemical properties of fenugreek seed aged under different level of osmotic potantials and temperatures.
  2. In areas with a lower osmotic potential it is better to cultivate Fenugreek seed at temperatures below 20 °C.

Effect of Osmotic Potential on Seed Germination and Biochemical Indices of different Christ's thorn (Ziziphus spina–christi) Populations
Mahnaz Mansouri, Ali Moradi, Hamidreza Balouchi, Elias Soltani,
Volume 8, Issue 1 ((Spring and Summer) 2021)
Abstract
Extended abstract
Introduction: Seed is the most important sexual reproductive factor in plants that plays an important role in transmitting hereditary characteristics, plant distribution, and establishment in different regions. Seed germination as one of the most important and complex stages in the life cycle of plants is affected by genetic and environmental factors. Drought stress is one of the main barriers of crop plants and trees, production in many parts of the world, especially in arid and semi-arid regions such as Iran. The purpose of this study was to investigate the effect of drought stress on biochemical and seed germination indices of different seed lot of Ziziphus spina–christi as suitable medicinal species for controlling soil erosion in arid regions.
Materials and Methods: This experiment was conducted as a factorial based on a completely randomized design with four replications at the Seed Science and Technology Laboratory of Yasouj University in 2018. Experimental factors included Ziziphus spina–Christi seeds collected from the regions of Minab, Kazeroon, Masjed Soleiman, and Dehdasht and five water potentials including zero, -2, -4, -6, and -8 bar.
Results: In the present study, the effect of osmotic potential, seed lot, and their interaction were significant in each seed lot on germination (germination percentage, germination rate, seedling length, allometric index, longitudinal vigor) and biochemical (hydrogen peroxide, soluble protein content, Proline, catalase, and ascorbate peroxidase) indices of Ziziphus spina–christi. The results showed that except for allometric index response of all studied germination traits to osmotic potential was decreasing, Also with increasing osmotic potential, seed soluble protein was decreased and the amount of proline and activity of catalase and ascorbate peroxidase increased. With increasing osmotic stress, the root length was significantly increased, which increased the allometric coefficient of the seedling.
Conclusion: Germination and biochemical indices of seeds of all seedlots had significant changes with the reduction of osmotic potential and the reduction of osmotic potential was associated with reduced germination and plant establishment capability. These results can help us to recognize the prerequisites of germination and the development of the forests.

Highlights:
1- Germination indices of seeds related to several Ziziphus spina–christi seed lots were investigated under different water potentials.
2- Biochemical aspects related to seed dormancy in several Ziziphus spina–christi seed lots were evaluated.

Germination and viability coefficients of Faba Bean (Vicia faba) seed under different storage conditions
Mohammad Mehrabi‌ Kooshki, Ali Moradi, Hamidreza Balouchi, Roya Behboud, Hojatollah Latifmanesh,
Volume 9, Issue 1 ((Spring and Summer) 2022)
Abstract
Extended Abstract
Introduction: Pulses are among the best sources of plant protein and important components of crop rotation, which in recent years, have been considered one of the major options for plant research. Seed storage is one of the important traits in legume breeding. Storage temperature, seed moisture content, and storage duration are the most important factors affecting seed quality during storage. Inappropriate storage conditions lead to deterioration and reduction of seed quality during storage, which is severely affected by the environmental conditions of storage.
Materials and Methods: This research was conducted at the Seed Technology Laboratory, Faculty of Agriculture, Yasouj University in 2014 as a three-way factorial based on the completely randomized design with 5 replications of 20 seeds. Seeds with moisture content at 5 levels (6, 10, 14, 18, and 22%) and storage temperature at 4 levels (15, 25, 35 and 45 °C) were stored for 9 months (0, 30, 60, 90, 120, 150, 180, 210, 240 and 270 days). After sampling at the end of each month, a standard seed germination test was done using the pleated paper method in a germinator at 25 °C for 10 days. Also, an electrical conductivity test of the electrolytes leaked from the seeds incubated for 24h in water at 20 ˚C was done with 4 replicates. Some germination attributes and electrical conductivity of the electrolytes leaked from the seeds were measured according to standard methods.
Results: According to the results, interaction effects of storage temperature, seed moisture content, and storage duration on germination indices and electrical conductivity of bean seeds were significant (P<0.1). The germination trend during storage at 15 °C and seed moisture content of 6% decreased from 94% to 81% after 270 days of storage, so that germination decreased to 35% under similar moisture content after 270 days of storage as temperature increased from 15 to 45 °C. As the storage time passed, electrical conductivity increased and this increase was more pronounced at higher temperatures. Viability constants were calculated 9 months after storage using the seed viability equation, in which KE, CH, CW, and CQ were calculated -5.39697, 0.03201, 2.13041, and 0.000017, respectively.
Conclusions: The results showed that the electrical conductivity of the leaked material increased with increasing storage temperature and seed moisture content, which led to lower viability of seeds. At 15 °C and 6% seed moisture content provided better conditions for seed survival during the 9-month storage time compared with all other temperatures and moistures and had the lowest rate of deterioration. The results showed that with increasing seed temperature and moisture so that they had to lowest electrical conductivity of the leaked material from seeds and deterioration rate.

Highlights:
1- Over storage duration, the electrical conductivity of materials leaked from seeds increased.
2- With increasing moisture content up to 22% and storage temperature up to 45 °C, the electrical conductivity of the material leaked from seeds increased.
3- Bean seed viability coefficients were calculated to evaluate seed viability under controlled storage conditions.
 
Revitalizing deteriorated sweet corn (Zea mays var. Saccharata Basin R) seeds through priming with ellagic acid, gibberellin, and potassium nitrate
Kamran Gharehbeygi Tavabea, Hamidreza Balouchi, Mohsen Movahhedi Dehnavi, Ali Moradi, Fatemeh Ebrahimi,
Volume 11, Issue 2 ((Autumn & Winter) 2025)
Abstract
Extended abstract
Introduction: Poor seedling vigor is one of the major challenges in agriculture, as it reduces seed germination capacity and seedling establishment, directly impacting plant establishment and yield. The use of seed priming methods with chemicals and growth regulators can serve as an effective strategy to enhance seedling vigor and improve biochemical traits and seed germination. In this study, the enhancement of sweet corn seedling vigor through priming with ellagic acid, gibberellin, and potassium nitrate was investigated under accelerated aging conditions.
Materials and Methods: A factorial experiment was conducted in the Seed Technology Laboratory of the Faculty of Agriculture at Yasouj University in 2024, based on a completely randomized design with two factors. The first factor was accelerated seed aging at two levels (aged and non-aged), and the second factor was seed priming at eight levels (no prime, hydropriming, ellagic acid at two concentrations (25 and 100 mg/l), gibberellin at two concentrations (50 and 150 mg/l), and potassium nitrate at two concentrations (15 and 60 mg/l)). Biochemical traits and seed germination characteristics were measured ultimately.
Results: The findings of this study revealed that seed deterioration significantly reduced germination characteristics, including germination percentage and rate, shoot length, root length, and seedling vigor index (length and weight). Additionally, biochemical indices such as soluble sugar content and α-amylase enzyme activity were negatively affected by aging. On the other hand, seed deterioration increased proline content, malondialdehyde levels, and peroxidase enzyme activity, indicating heightened oxidative stress under aging conditions. Priming of deterioration seeds with ellagic acid, gibberellin, and potassium nitrate positively influenced germination characteristics and biochemical indices. Specifically, concentrations of 100 mg/l ellagic acid, 150 mg/l gibberellin, and 15 mg/l potassium nitrate were the most effective pretreatments.
Conclusion: The results of this research demonstrated that pretreatment of deteriorated Basin sweet corn seeds with ellagic acid, gibberellin, and potassium nitrate significantly improved germination and biochemical indices. These treatments mitigated the negative effects of seed aging, enhancing seedling vigor and establishment. Therefore, these priming can be recommended as effective methods to improve the quality of Basin sweet corn seeds under various agricultural conditions.

Highlights:
  1. Seed deterioration leads to an increase in biochemical indicators such as seed proline content, peroxidase enzyme activity, and seed malondialdehyde content.
  2. The vigor of Basin sweet corn seeds is improved through pretreatment with gibberellin and ellagic acid.
  3. The effect of priming before and after artificial deterioration on germination and seed vigor of sweet corn cultivar Basin was compared and investigated.

Optimizing Sugarcane Cultivation: Efficacy of Single-Bud Pelleting on Emergence and Quality Traits
Zahra Ajribzadeh, Salim Farzaneh, Mahmoud Shomeili, Hamidreza Balouchi, Raouf Seyed Sharifi, Aziz Karmollachaab,
Volume 12, Issue 1 ((Spring and Summer) 2025)
Abstract
Objective: Commercial sugarcane cultivation is often associated with high production costs due to the extensive use of planting material, energy waste, long cultivation periods, rapid deterioration of cuttings after bud establishment, labor-intensive operations, frequent machinery use, and heavy applications of fertilizers and pesticides. This study evaluated the efficacy of pelleting lateral buds from sugarcane stalks to produce single-bud seed pieces suitable for mechanized cultivation at the Sugarcane Development, Research, and Training Institute in Khuzestan, Iran.
Methods: A split-plot experiment, based on a randomized complete block design with three replications, was conducted in 2021 at the Sugarcane Development, Research, and Training Institute (SDRTI) in Khuzestan, Iran. The factors considered were different planting dates—16 August (D1), 16 September (D2), and 17 October (D3)—as the main plots, and pellet types (A1: starch + peat + micro-combi fertilizer; A2: starch + peat + nutritional compounds + potassium silicate + carbendazim fungicide; A3: single-bud without pellet; and A4: 50 setts without pellet as the control) as the subplots.
Results: Pelleting single-bud seeds, particularly in treatment A2 planted in September, significantly enhanced crop establishment. It increased the emergence percentage by 56% and the emergence rate to 2.85 sprouts per day compared to the control. Pelleting also improved growth and quality indices more effectively than the other treatments.
Conclusions: Pelleting single-bud sugarcane seeds with nutrient-enriched coatings improves emergence, supports early crop growth, and facilitates mechanized cultivation. This technique supports mechanized planting by enhancing nutrient delivery and moisture retention at the bud level, contributing to more sustainable, efficient, and cost-effective sugarcane cultivation.

Highlights:
  • Using treatment A2 for pelleting significantly boosted the emergence rate of sugarcane single buds by providing essential nutrients.
  • Planting in September along with pelleting improved the early growth and establishment of sugarcane plants.

Mepiquat Chloride (Pix) Improves Germination and Biochemical Stability of Sesame (Sesamum indicum cv. Darab-1) Seeds under Deterioration
Ramin Zeilabpour, Ali Moradi, Hamidreza Balouchi, Alireza Yadavi, Hojatollah Latifmanesh,
Volume 12, Issue 2 ((Autumn & Winter) 2026)
Abstract
Objective: Seed aging in oilseeds such as sesame is a major storage challenge, reducing germination quality and seedling vigor. The high-oil cultivar Darab‑1 is highly sensitive to storage conditions (temperature, moisture, and time). In this study, the effects of different Pix concentrations in interaction with temperature, moisture, and storage duration were investigated on germination and biochemical traits of seeds to determine the possibility of improving seed quality stability during storage.
Methods: The experiment was conducted as a four‑factor factorial arrangement in a completely randomized design with three replications. The studied factors were storage temperature (15, 25, and 35°C), seed moisture content (9, 12, and 15%), Pix concentration (0, 3000, and 4500  mg L-1), and storage duration (30, 60, 90, 120, 150, and 180 days). After the storage period, seed physiological traits including germination percentage and rate, root and shoot length, seedling dry weight, vigor index, α‑amylase activity, and electrolyte leakage were measured.
Results: Increasing temperature, moisture, and storage duration accelerated seed aging. After 180 days, electrolyte leakage in control seeds increased from 21.47 to 72.72% when storage temperature rose from 15 to 25°C (at 9% moisture), indicating a 54% increase in membrane leakage, while Pix at 3000 mg/L reduced it by 19–80%. α-amylase activity decreased with seed deterioration, but Pix at 3000 mg/L maintained the highest enzyme activity (15.5 vs. 16.3 nmol seed-1min-1 after 180 days). Germination percentage and rate, seedling length and dry weight, and vigor index also declined with aging; however, Pix treatment, especially at 3000 mg L-1, significantly moderated these reductions.
Conclusions: Storage at high temperature and moisture accelerated sesame seed deterioration, reducing germination and α‑amylase activity while increasing membrane damage. Pix at 3000 mg L-1 was the most effective treatment, maintaining seed vigor and enzyme activity and reducing electrolyte leakage. Therefore, seed treatment with 3000 mg L-1 Pix before storage, especially at 15°C and 9% moisture content, can be recommended as an effective strategy to extend seed longevity and maintain quality during medium term storage.

Highlights:
  • Pix at 3000 mg/L effectively prevented the decline in physiological traits of sesame seeds during storage and maintained germination potential and seed vigor.
  • Increased temperature, moisture, and storage duration reduced α‑amylase activity and increased electrolyte leakage, indicating accelerated physiological seed aging.
  • Pix treatment improved seed quality under warm and humid storage conditions by reducing electrolyte leakage and enhancing membrane stability, suggesting its practical application for extending seed longevity.

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