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Shirin Taghi Zoghi , Elias Soltani , Iraj Alahdadi , Reza Sadeghi ,
Volume 4, Issue 2 ((Autumn & Winter) 2018)
Abstract

This study was conducted to study the effects of different priming methods on germination rate and percentage under salinity stress and to determine the stability of primed seeds. In order to accomplish this, three different experiments were conducted separately, including the experiment of water uptake, the experiment of salinity stress, and the experiment of storability of primed seeds. Priming treatments were five levels of control (unprimed), hydropriming (Hyd), priming with humic acid (HA), priming with salicylic acid (SA) and priming with gibberellic acid (GA). Salinity stresses were four levels of 0, 4, 8 and 12 ds/m of NaCl. The stability of prime seeds was investigated over a period of 226 days after priming. The results of water uptake showed that rapeseeds entered into the third phase of water uptake after 18 hours of hydration. The results of the salinity experiment showed that salinity levels of 12 and 0 ds/m had the lowest (74.3 %) and highest (83 %) germination percentage, respectively. In terms of germination rate, there were significant differences between GA (0.034 h-1), HA (0.036 h-1) and Hyd (0.036 h-1) with C (0.019 h-1) and SA (0.027 h-1). Generally speaking, primed seeds germinated better than control seeds at all levels of salinity. The storability of primed seeds and control seeds had no significant decrease during storage. Finally, it was concluded that seed priming increased the tolerance to salinity stress; in terms of storability, there was no significant difference between primed seeds and primed seeds could be stored in the same way as control seeds.

Highlights:

  1. At the current research, the stability of prime seeds was investigated for the first time.
  2. There was no significant difference between the storability of primed seeds and control (unprimed) at each sampling time (with an exception for SA).
  3. Primed seeds had better germination performance than control at the all salinity stress levels.
  4. Seed priming treatments using gibberellic acid, humic acid and hydropriming were the best compared with the other treatments.

Keyvan Maleki, Elias Soltani, Iraj Alahdadi, Majid Ghorbani Javid,
Volume 6, Issue 2 ((Autumn & Winter) 2020)
Abstract



Extended abstract
Introduction: Conditional dormancy (CD) is a dynamic state between dormancy (D) and nondormancy (ND). Seeds at the conditional dormancy stage germinate over a narrower range of temporal conditions. Conditional dormancy is usually observed in seeds with physiological dormancy. However, primary conditional dormancy has also been seen in some freshly harvested seeds. The purpose of the present study was to investigate whether freshly harvested oilseeds have non-dormancy or conditional dormancy.
Materials and Methods: A factorial experiment was conducted based on a completely randomized design with four replications at Seed Technology Laboratory of Aburaihan Campus, University of Tehran, Iran, in 2018. In this experiment, seeds of rapeseed were collected from 20 different locations in Golestan and Mazandaran provinces. Following that, a germination test was carried out at different temperatures (5, 15, 20, 30, 35°C), and the germination percentage and seed germination rate were recorded. In order to break seed dormancy, two treatments were used: gibberellic acid and after-ripening. For after-ripening treatment, seeds were stored in a paper bag in a dry and dark environment for 6 months. For gibberellic acid treatment, a solution of 100 parts per million (PPM) of gibberellic acid was prepared and added to the Petri dishes. Subsequently, the percentage and rate of germination were recorded.
Results: The results showed that freshly harvested seeds had primary conditional dormancy and germinated in a narrow range of temporal conditions. In addition, cardinal temperatures for freshly harvested seeds were 4.45 and 27.8 for bases and ceilings, respectively. Following gibberellic acid and after-ripening treatments, seeds germinated in a wider range of temperatures and base and ceiling temperatures reached 1.74 and about 40°C, respectively. Thus, germination percentage of seeds treated with gibberellic acid and after-ripening increased at both high and low temperatures. However, the increase in germination percentage was higher at high temperatures than low temperatures. In addition, the effect of gibberellic acid treatment was more than that of after-ripening treatment on the release of dormancy, and after-ripening treatment had an intermediate effect between the gibberellic acid and freshly harvested seeds.
Conclusion: Based on the results of this experiment, the application of gibberellic acid and after-ripening treatments resulted in breaking the dormancy of freshly harvested seeds and increased germination temperature range at high and low temperatures.  Of the two treatments, gibberellic acid had the greatest effect on breaking dormancy and increasing temperature range. Among the cultivars, these changes were maximum in the germination capacity of Hyola 50 and Trapar cultivars and Trapar cultivar had minimum changes.
 
 
Highlights:
1-Conditional dormancy of oilseed cultivars was investigated under different environmental conditions.
2-Application of gibberellic acid and after-ripening treatments resulted in breaking primary conditional dormancy in oilseed cultivars.


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