Volume 12, Issue 2 ((Autumn & Winter) 2026)
Iranian J. Seed Res. 2026, 12(2): 69-91 |
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Saadat H, Sedghi M. (2026). Mitigating the Adverse Impact of Salinity Stress on Germination of Wheat (Triticum aestivum cv. Sain) through Seed Priming with Chitosan Nanoparticles. Iranian J. Seed Res.. 12(2), 69-91.
URL: http://yujs.yu.ac.ir/jisr/article-1-652-en.html
URL: http://yujs.yu.ac.ir/jisr/article-1-652-en.html
Department of Agronomy, Faculty of Agriculture and Natural Resources, University of Mohaghegh Ardabili , t.saadat@uma.ac.ir
Abstract: (81 Views)
Objective: This study aimed to investigate the reduction of adverse effects of salinity stress on germination and related traits of wheat (Triticum aestivum cv. Sain) through seed priming with chitosan nanoparticles.
Method: The experiment was conducted as a factorial arrangement based on a completely randomized design with three replications at the University of Mohaghegh Ardabili in 2025. Treatments included four salinity levels (0, 50, 100, and 150 mM NaCl) and four levels of chitosan nanoparticles (0, 0.1, 0.2, and 0.5% weight/volume dissolved in 1% acetic acid).
Results: Germination percentage (GP) and plumule fresh weight (PFW) decreased at 150 mM salinity compared to the control (without salinity), but seed priming with 0.5% chitosan nanoparticles improved GP and PFW at the same salinity level by about 20% and 50%, respectively, compared to no chitosan application. Radicle fresh weight (RFW), radicle dry weight (RDW), plumule dry weight (PDW), seedling length vigor index (SLVI), and seedling weight vigor index (SWVI) in the 0.5% chitosan nanoparticle treatment without salinity increased by 77, 72, 83, 76, and 89%, respectively, compared to hydropriming under 150 mM salinity. With increasing salinity, proline content increased, but the application of 0.5% chitosan nanoparticles increased this trait by 32% compared to hydropriming. Protein content under no salinity and priming with 0.5% chitosan nanoparticles increased by about 76% compared to hydropriming under 150 mM salinity. Also, soluble sugar content in the treatment with 0.5% chitosan nanoparticles under 150 mM salinity increased by about 84% compared to hydropriming without salinity.
Conclusions: The results showed that the use of 0.5% chitosan nanoparticles improved germination indices and biochemical traits of wheat seeds under salinity stress.
Highlights
Method: The experiment was conducted as a factorial arrangement based on a completely randomized design with three replications at the University of Mohaghegh Ardabili in 2025. Treatments included four salinity levels (0, 50, 100, and 150 mM NaCl) and four levels of chitosan nanoparticles (0, 0.1, 0.2, and 0.5% weight/volume dissolved in 1% acetic acid).
Results: Germination percentage (GP) and plumule fresh weight (PFW) decreased at 150 mM salinity compared to the control (without salinity), but seed priming with 0.5% chitosan nanoparticles improved GP and PFW at the same salinity level by about 20% and 50%, respectively, compared to no chitosan application. Radicle fresh weight (RFW), radicle dry weight (RDW), plumule dry weight (PDW), seedling length vigor index (SLVI), and seedling weight vigor index (SWVI) in the 0.5% chitosan nanoparticle treatment without salinity increased by 77, 72, 83, 76, and 89%, respectively, compared to hydropriming under 150 mM salinity. With increasing salinity, proline content increased, but the application of 0.5% chitosan nanoparticles increased this trait by 32% compared to hydropriming. Protein content under no salinity and priming with 0.5% chitosan nanoparticles increased by about 76% compared to hydropriming under 150 mM salinity. Also, soluble sugar content in the treatment with 0.5% chitosan nanoparticles under 150 mM salinity increased by about 84% compared to hydropriming without salinity.
Conclusions: The results showed that the use of 0.5% chitosan nanoparticles improved germination indices and biochemical traits of wheat seeds under salinity stress.
Highlights
- Seed priming with 0.5% chitosan nanoparticles improved some germination indices of wheat (cv. Sain) seeds under salinity stress.
- Seed priming with 0.5% chitosan nanoparticles increased proline, protein, and soluble sugar contents in wheat (cv. Sain) seedlings.
Type of Study: Research |
Subject:
General
Received: 2025/09/30 | Revised: 2026/02/26 | Accepted: 2026/03/16 | ePublished: 2026/03/20
Received: 2025/09/30 | Revised: 2026/02/26 | Accepted: 2026/03/16 | ePublished: 2026/03/20
References
1. Abdeen, S.A., Hefni, H.H. & Mady, A.H.J. (2023). Foliar application of chitosan zinc oxide nanoparticles on wheat productivity and water use efficiency under deficit irrigation water. Journal of Central European Agriculture, 24(2), 476-490. [DOI:10.5513/JCEA01/24.2.3639]
2. Abdolahipour, B. & Haghighi, M. (2019). The effect of pine wood vinegar on germination, growth and physiological characteristics, and uptake of elements in Basil. Journal of Science and Technology of Greenhouse Culture, 10(2), 11-24. [In Persian]. [DOI:10.29252/ejgcst.10.2.11]
3. Abdul-Baki, A.A. & Anderson, J.D. (1973). Vigor determination in soybean by multiple criteria. Crop Science, 13, 630-633. [DOI:10.2135/cropsci1973.0011183X001300060013x]
4. Ahmadvand, B., Sharifzadeh, F. & Mirabzadeh Ardakani, M. (2023). The effect of hydro and osmo priming treatments on germination traits enhancement of Sesamum indicum L var. shevin seeds under drought stress. Iranian Journal of Seed Science and Technology, 11(4), 1-16. [In Persian]. [DOI:10.22092/ijsst.2022.359726.1446 [In Persian].]
5. Ali, L.G., Nulit, R., Ibrahim, M.H. & Yien, C.Y.S. (2021). Efficacy of KNO3, SiO2 and SA priming for improving emergence, seedling growth and antioxidant enzymes of rice (Oryza sativa), under drought. Scientific Reports, 11, 3864. [DOI:10.1038/s41598-021-83434-3] [PMID] [PMCID]
6. Ali, Q., Daud, M.K., Haider, M.Z., Ali, S., Rizwan, M., Aslam, N., Noman, A., Iqbal, N., Shahzad, F., Deeba, F., Ali, I., &, Zhu, S.J. (2017). Seed priming by sodium nitroprusside improves salt tolerance in wheat (Triticum aestivum L.) by enhancing physiological and biochemical parameters. Plant Physiology and Biochemistry, 119, 50-58. [DOI:10.1016/j.plaphy.2017.08.010] [PMID]
7. Ali. F., Bano, A., Hassan, T.U., Nazir, M. & Khan, R.T. (2023). Plant growth promoting rhizobacteria induced modulation of physiological responses in rice under salt and drought stresses. Pakistan Journal of Botany, 55(2), 447-52. [DOI:10.30848/PJB2023-2(23)]
8. Arora, N.K. (2019). Impact of climate change on agriculture production and its sustainable solutions. Environmental Sustainability, 2, 95-96. [DOI:10.1007/s42398-019-00078-w]
9. Bahrasemani, S., A. Seyedi, S.H. Fathi. & M. Jowkar. (2023). The seed priming using putrescine improves, germination indices and seedlings morphobiochemical responses of Indigo (Indigofera tinctoria) under salinity stress. Journal of Medicinal Plants and By-Products, 13(1), 179-188. [DOI:10.22034/JMPB.2023.128870]
10. Bakhoum, G.S.H., Sadak, M.S.H. & Tawfik, M.M. (2022). Chitosan and chitosan nanoparticle effect on growth, productivity and some biochemical aspects of Lupinus termis L. plant under drought conditions. Egyptian Journal of Chemistry, 65(5), 537-549. [DOI:10.21608/ejchem.2021.97832.4563]
11. Bates, L.S., Waldern, R.P. & Tear, I.D. (1973). Rapid determination of free proline for water stress studies. Plant and Soil, 39, 205-207. [DOI:10.1007/BF00018060]
12. Bewley, J.D., Bradford, K. & Hilhorst, H. (2012). Seeds: physiology of development, germination and dormancy. Springer Press Inc., New York. [DOI:10.1007/978-1-4614-4693-4]
13. Bradford, M. (1976). Arapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein day binding. Analytical Biochemistry, 72, 248-254. [DOI:10.1016/0003-2697(76)90527-3] [PMID]
14. Dawood, M.G., El-Awadi, M.E. & Sadak, M.S. (2024). Chitosan and its nanoform regulates physiological processes and antioxidant mechanisms to improve drought stress tolerance of Vicia faba plant. Journal of Soil Science and Plant Nutrition, 24(3), 5696-5709. [DOI:10.1007/s42729-024-01934-3]
15. Divya, K., Vijayan, S., Nair, S.J. & Jisha, M.S. (2019). Optimization of chitosan nanoparticle synthesis and its potential application as germination elicitor of Oryza sativa L. International Journal of Biological Macromolecules, 124, 1053-1059. [DOI:10.1016/j.ijbiomac.2018.11.185] [PMID]
16. Dubey, A.K., Kumar, N., Kumar, A., Ansari, M.A., Ranjan, R., Gautam, A., Sahu, N., Pandey, V., Behera, S.K. & Mallick, S. (2019). Over-expression of CarMT gene modulates the physiological performance and antioxidant defense system to provide tolerance against drought stress in Arabidopsis thaliana L. Ecotoxicology and Environmental Safety, 171, 54-65. [DOI:10.1016/j.ecoenv.2018.12.050] [PMID]
17. Ebrahimi, O., Esmaili, M.M., Sabori, H. & Tahmasebi, A. (2013). Effects of salinity and drought stress on germination two spices of (Agropyron elongatum, Agropyron desertrum). Desert Ecosystem Engineering Journal, 1(1), 31-38. [In Persian]
18. Edo, G.I., Yousif, E. & Al-Mashhadani M.H. (2024). Chitosan: An overview of biological activities, derivatives, properties, and current advancements in biomedical applications. Carbohydrate Research, 542, 109199. [DOI:10.1016/j.carres.2024.109199] [PMID]
19. El-Hendawy, S., Elshafei, A., Al-Suhaibani, N., Alotabi, M., Hassan, W. & Dewir, Y.H. (2019). Assessment of the salt tolerance of wheat genotypes during the germination stage based on germination ability parameters and associated SSR markers. Journal of Plant Interactions, 14(1), 151-163. [DOI:10.1080/17429145.2019.1603406]
20. Elsharkawy, M.M., Omara, R.I., Mostafa, Y.S., Alamri, S.A., Hashem, M., Alrumman, S.A. & Ahmad, A.A. (2022). Mechanism of wheat leaf rust control using chitosan nanoparticles and salicylic acid. Journal of Fungi, 8(3), 304. [DOI:10.3390/jof8030304] [PMID] [PMCID]
21. Evlice, A.K. & Zencirci, N. (2025). Securing Wheat Cultivation for Global Food Safety. In: Zencirci, N. (eds.), Empowering Wheat Cultivation with GIS, Digital Approaches and Artificial Intelligence, pp. 1-13. [DOI:10.1007/978-3-031-99954-3_1]
22. Farooq, T., Un Nisa, Z., Hameed, A., Ahmed, T. & Hameed, A. (2022). Priming with copper-chitosan nanoparticles elicit tolerance against PEG-induced hyperosmotic stress and salinity in wheat. BMC Chemistry, 16, 23. [DOI:10.1186/s13065-022-00813-1] [PMID] [PMCID]
23. Ghosh, U.K., Islam, M.N., Siddiqui, M.N., Cao, X. & Khan. M.A.R. (2022). Proline, a multifaceted signalling molecule in plant responses to abiotic stress: understanding the physiological mechanisms. Plant Biology, 24, 227- 239. [DOI:10.1111/plb.13363] [PMID]
24. Guan, Y.J., Hu, J., Wang, X.J. & Shao, C.X. (2009). Seed priming with chitosan improves maize germination and seedling growth in relation to physiological changes under low temperature stress. Journal of Zhejiang University Science B, 10, 427-433. [DOI:10.1631/jzus.B0820373] [PMID] [PMCID]
25. Gupta, S., Schillaci, M., Walker, R., Smith, P.M., Watt, M. & Roessner, U. (2021). Alleviation of salinity stress in plants by endophytic plant-fungal symbiosis: Current knowledge, perspectives and future directions. Plant and Soil, 461, 219-244. [DOI:10.1007/s11104-020-04618-w]
26. Guru, G.R., Ramteke, P.W., Veres, C. & Vágvölgyi, C. (2025). Potential impacts of nanoparticles integration on micropropagation efficiency: current achievements and prospects. Frontiers in Plant Science, 7(16), 1629548. [DOI:10.3389/fpls.2025.1629548] [PMID] [PMCID]
27. Hameed, A., Maqsood, W. & Hameed, A. (2024). Chitosan nanoparticles encapsulating curcumin counteract salt-mediated ionic toxicity in wheat seedlings: an ecofriendly and sustainable approach. Environmental Science and Pollution Research, 31, 8917-8929. [DOI:10.1007/s11356-023-31768-y] [PMID]
28. Hidangmayum, A., Dwivedi, P., Katiyar, D. & Hemantaranjan, A. (2019). Application of chitosan on plant responses with special reference to abiotic stress. Physiology and Molecular Biology of Plants, 25(2), 313-326. [DOI:10.1007/s12298-018-0633-1] [PMID] [PMCID]
29. Hossen, M.S., Karim, M.F., Fujita, M., Bhuyan, M.H.M.B., Nahar, K. & Masud, A.A.C. (2022). Comparative physiology of Indica and Japonica rice under salinity and drought stress: an intrinsic study on osmotic adjustment, oxidative stress, antioxidant defense and methylglyoxal detoxification. Stresses, 2(2), 156-178. [DOI:10.3390/stresses2020012]
30. Huang, S., Gill, S., Ramzan, M., Ahmad, M.Z., Danish, S. & Huang, P. (2023). Uncovering the impact of AM fungi on wheat nutrient uptake, ion homeostasis, oxidative stress, and antioxidant defense under salinity stress. Scientific Reports, 13, 8249. [DOI:10.1038/s41598-023-35148-x] [PMID] [PMCID]
31. Hussain, S., Shaukat, M., Ashraf, M., Zhu, C., Jin. Q. & Zhang. J. (2019). Salinity Stress in Arid and Semi-Arid Climates: Effects and Management in Field Crops. In Climate Change and Agriculture. IntechOpen. [DOI:10.5772/intechopen.87982]
32. Igrejas, G. & Branlard, G. (2020). The Importance of Wheat. In: Igrejas, G., Ikeda, T.M. & Guzman, C. (eds.), Wheat Quality for Improving Processing and Human Health, Springer, 1-7. [DOI:10.1007/978-3-030-34163-3_1]
33. Iqbal, S., Hussain, S., Abdul Qayyaum, M., Ashraf, M., & Saifullah. (2021). The Response of Maize Physiology under Salinity Stress and Its Coping Strategies. In Plant Stress Physiology. IntechOpen. [DOI:10.5772/intechopen.92213]
34. ISTA. 2013. International Rules for Seed Testing. Bassersdorf, Switzerland: The International Seed Testing Association (ISTA).
35. Kaur, S., Suhalia, A., Sarlach, R. S., Mohd, S., Pritpal, S., Gomti, G., Anureet, B. & Achla, S. (2022). Uncovering the Iranian wheat landraces for salinity stress tolerance at early stages of plant growth. Cereal Research Communications, 50, 895-904. [DOI:10.1007/s42976-022-00245-6]
36. Kheiri, A., Jorf, S.A.M., Malihipour, A., Saremi, H. & Nikkhah. M. (2016). Application of chitosan and chitosan nanoparticles for the control of Fusarium head blight of wheat (Fusarium graminearum) in vitro and greenhouse. International Journal of Biological Macromolecules, 93, 1261-1272. [DOI:10.1016/j.ijbiomac.2016.09.072] [PMID] [PMCID]
37. Kochert, G. (1978). Carbohydrate Determination by Phenol-Sulfuric Acid Method. In: Hellebust, J.A. & Craige, J.S. (eds.), Physiological and Biochemical Methods, pp. 95-97.
38. Kubala, S., Garnczarska, M. & Wojtyla, L. (2015). Deciphering priming-induced improvement of rapeseed (Brassica napus L.) germination through an integrated transcriptomic and proteomic approach. Plant Science, 231, 94-113. [DOI:10.1016/j.plantsci.2014.11.008] [PMID]
39. Li, R., He, J., Xie, H., Wang, W., Bose, S.K., Sun, Y., Hu, J. & Heng Yin, H. (2019). Effects of chitosan nanoparticles on seed germination and seedling growth of wheat (Triticum aestivum L.). International Journal of Biological Macromolecules, 126, 91-100. [DOI:10.1016/j.ijbiomac.2018.12.118] [PMID]
40. Malerba, M. & Cerana, R. (2016). Chitosan effects on plant systems. Journal of Cell Science and Molecular Biology, 17(7), 996. [DOI:10.3390/ijms17070996] [PMID] [PMCID]
41. Naseer, M.N., Rahman, F.U., Hussain, Z., Khan, I.A., Aslam, M.M., Aslam, A., Waheed, H., Khan, A.U. & Iqbal, S. (2022). Effect of salinity stress on germination, seedling growth, mineral uptake and chlorophyll contents of three Cucurbitaceae species. Brazilian Archives of Biology and Technology, 65, e22210213. [DOI:10.1590/1678-4324-2022210213]
42. Nile, S.H., Thiruvengadam, M. & Wang, Y. (2022). Nano-priming as emerging seed priming technology for sustainable agriculture-recent developments and future perspectives. Journal of Nanobiotechnology, 20, 254. [DOI:10.1186/s12951-022-01423-8] [PMID] [PMCID]
43. O'Hara, L.E., Paul, M.J. & Wingler, A. (2013). How do sugars regulate plant growth and development? New insight into the role of trehalose-6-phosphate. Molecular Plant, 6(2), 261-274. [DOI:10.1093/mp/sss120] [PMID]
44. Omar, H.S., Al Mutery, A., Osman, N.H., Reyad, N.E.H.A. & Abou-Zeid, M.A. (2021). Genetic diversity, antifungal evaluation and molecular docking studies of Cu-chitosan nanoparticles as prospective stem rust inhibitor candidates among some Egyptian wheat genotypes. PLoS ONE, 16, e0257959. [DOI:10.1371/journal.pone.0257959] [PMID] [PMCID]
45. Omara, A. E.-D., Hafez, E. M., Osman, H. S., Rashwan, E., El-Said, M. A. A., Alharbi, K., Abd El-Moneim, D., & Gowayed, S. M. (2022). Collaborative impact of compost and beneficial rhizobacteria on soil properties, physiological attributes, and productivity of wheat subjected to deficit irrigation in salt affected soil. Plants, 11(7), 877. [DOI:10.3390/plants11070877] [PMID] [PMCID]
46. Omidi, H., Leyla, J. & Hasanali, N. 2014. Seeds of Medicinal Plants and Crops. Natural Resources and Environment, 269-189.
47. Raeisi Sadati, S.Y., Jahanbakhsh Godehkahriz, S., Ebadi, A. & Sedghi, M. (2022). Zinc oxide nanoparticles enhance drought tolerance in wheat via physio-biochemical changes and stress genes expression. Iranian Journal of Biotechnology, 20(1), 12-24. [DOI:10.30498/ijb.2021.280711.3027 [In Persian]]
48. Rakgotho, T., Ndou, N., Mulaudzi, T., Iwuoha, E., Mayedwa, N. & Ajayi, R.F. (2022). Green-synthesized zinc oxide nanoparticles mitigate salt stress in sorghum bicolor. Agriculture, 12(5), 597. [DOI:10.3390/agriculture12050597]
49. Rhaman, M.S., Rauf, F., Tania, S.S. & Khatun, M. (2020). Seed priming methods: Application in field crops and future perspectives. Asian Journal of Research in Crop Science, 25(2), 8-19. [DOI:10.9734/ajrcs/2020/v5i230091]
50. Saadat, H. & Sedghi, M. (2024a). The effect of seed priming with chitosan on the improvement of physiological and biochemical traits of soybean (Glycine max (L.) Merrill) under salinity stress. Russian Journal of Plant Physiology, 71, 187. [DOI:10.1134/S1021443724605858]
51. Saadat, H. & Sedghi, M. (2024b). The effect of salicylic acid on germination indices in wheat seedlings (Triticum aestivum L.) under salinity stress. Seed Research, 13 (4), 1-16. [In Persian].
52. Saadat, H. & Sedghi, M. (2025a). Effect of seed priming with salicylic acid on germination and biochemical traits of bread wheat (Triticum aestivum cv. Sain) under salinity stress. Iranian Journal of Seed Research, 11(2), 111-124.
https://doi.org/10.61882/yujs.11.2.111 [DOI:10.61882/yujs.11.2.111 [In Persian].]
53. Saadat, H. & Sedghi, M. (2025b).The effect of seed priming with chitosan on physiological and biochemical characteristics of pea (Pisum sativum L.) seedlings under rapid aging test. Russian Journal of Plant Physiology, 72, 119. [DOI:10.1134/S1021443725600606]
54. Saadat, H., Sedghi, M., Seyed Sharifi, R. & Farzaneh, S. (2023a). Expression of gibberellin synthesis genes and antioxidant capacity in common bean (Phaseolus vulgaris L. cv. Sadri) seeds induced by chitosan under salinity. Iranian Journal of Plant Physiology, 13(4), 4715-4728. [DOI:10.30495/ijpp.2023.1978837.1460]
55. Saadat, H., Sedghi, M., Seyed Sharifi, R. & Farzaneh, S. (2023b). The effect of priming with different levels of chitosan on physiological and biochemical traits in French bean (Phaseolus vulgaris L.) under salinity stress. Plant Production Technology, 14(2), 75-89.
https://doi.org/10.61186/yujs.10.2.21 [DOI:10.22084/PPT.2023.26100.2075 [In Persian].]
56. Saadat, H., Soltani, E. & Sedghi, M. (2023c). The effect of seed priming with chitosan on germination characteristics and activity of antioxidant enzymes in rice seedlings (Oryza sativa L.) under salinity stress. Journal of Plant Process and Function, 12(54), 239-258. [In Persian].
57. Saadat, T., Sedghi, M., Seyed Sharifi, R. & Farzaneh, S. (2023d). Effect of chitosan on germination indices of common bean (Phaseolus vulgaris) (cv. Sedri) seeds under salt stress. Iranian Journal of Seed Research, 9(2): 151-162.
https://doi.org/10.61186/yujs.9.2.151 [DOI:10.61186/yujs.9.2.151 [In Persian].]
58. Saadat, T., Sedghi, M., Seyed Sharifi, R. & Farzaneh, S. (2024). The effect of chitosan priming characteristics on germination indices and biochemical bean seeds (Phaseolus vulgaris cv. Sadri) under salinity stress. Iranian Journal of Seed Research, 10(2), 21-36.
https://doi.org/10.61186/yujs.10.2.21 [DOI:10.61186/yujs.10.2.21 [In Persian].]
59. Sadeghipour, O. (2021). Chitosan application improves nickel toxicity tolerance in soybean. Journal of Soil Science and Plant Nutrition, 21(3), 2096-2104. [DOI:10.1007/s42729-021-00505-0]
60. Sali, S., Thampi, M. & Jisha, M.S. (2026). Chitosan-based nanocomposites: A sustainable frontier in plant growth regulation and postharvest preservation. Physiological and Molecular Plant Pathology, 141, 103026. [DOI:10.1016/j.pmpp.2025.103026]
61. Soni, A.T., Rookes, J.E. & Arya, S.S. (2023). Chitosan nanoparticles as seed priming agents to alleviate salinity stress in rice (Oryza sativa L.) seedlings. Polysaccharides, 4(2), 129-141. [DOI:10.3390/polysaccharides4020010]
62. Steven, S., Islam, M.S., Ghimire, A., Methela, N.J., Kwon, E.H., Yun, B.W., Lee, I.J., Kim, S.H. & Kim, Y. (2024). Chitosan-GSNO Nanoparticles and Silicon Priming Enhance the Germination and Seedling Growth of Soybean (Glycine max L.). Plants, 13(10), 1290. [DOI:10.3390/plants13101290] [PMID] [PMCID]
63. Szepesi, Á. & Szőllősi, R. (2018). Chapter 17 - Mechanism of proline biosynthesis and role of proline metabolism enzymes under environmental stress in plants. Plant Metabolites and Regulation under Environmental Stress, 11, 337-353. [DOI:10.1016/B978-0-12-812689-9.00017-0]
64. Tao, Q., Lv, Y., Mo, Q., Bai, M., Han, Y. & Wang, Y. (2018). Impacts of priming on seed germination and seedling emergence of Cleistogenes songorica under drought stress. Seed Science and Technology, 46(2), 239-258. [DOI:10.15258/sst.2018.46.2.06]
65. Uçarlı, C. (2020). Effects of Salinity on Seed Germination and Early Seedling Stage. In Abiotic Stress in Plants. IntechOpen.
https://doi.org/10.5772/intechopen.93647 [DOI:10.5772/intechopen.93647.]
66. Waqas, M., Korres, N.E., Khan, M.D., Nizami, A.S., Deeba, F., Ali, I. & Hussain, H. (2019). Advances in the Concept and Methods of Seed Priming. In: Hasanuzzaman, M., Fotopoulos, V. (eds.), Priming and Pretreatment of Seeds and Seedlings, pp. 11-41. [DOI:10.1007/978-981-13-8625-1]
67. Zafar, S., Khan, S., Ibrar, D. & Khan. M. K. (2024). Application of zinc nanoparticles as seed priming agent improves growth and yield of wheat seedlings grown under salinity stress by enhanced antioxidants activities and gas exchange attributes. Cereal Research Communications, 52, 1551-1564. [DOI:10.1007/s42976-024-00499-2]
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