Volume 9, Issue 2 ((Autumn & Winter) 2023)
Iranian J. Seed Res. 2023, 9(2): 17-34 |
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Barahouei M, Moosavi S G, Seghatoleslami M J, Baradaran R, Javadzadeh S M. (2023). Effect of seed priming on some germination indices and enzymatic activity of safflower (Carthamus tinctorius) seedling under drought stress in vitro. Iranian J. Seed Res.. 9(2), : 2 doi:10.61186/yujs.9.2.17
URL: http://yujs.yu.ac.ir/jisr/article-1-548-en.html
URL: http://yujs.yu.ac.ir/jisr/article-1-548-en.html
Mansoor Barahouei 
, Seyyed Gholamreza Moosavi * , Mohamad Javad Seghatoleslami , Reza Baradaran , Seyyed Mahdi Javadzadeh
, Seyyed Gholamreza Moosavi * , Mohamad Javad Seghatoleslami , Reza Baradaran , Seyyed Mahdi Javadzadeh
Birjand Branch, Islamic Azad University , moosavi@iaubir.ac.ir
Abstract: (1873 Views)
Extended Abstract
Introduction: Safflower is a plant that has been considered due to its high medicinal and nutritional value, especially in the extraction of edible oils in developed countries. Drought is one of the most important harmful factors in arid and semi-arid regions of the world that affects plant production. Modifiers play an important role in plant adaptation to stress conditions. Among these compounds are the hormone gibberellic acid and the antioxidant ascorbic acid, which increase plant tolerance to adverse environmental conditions. The present study investigated the effect of gibberellic acid and ascorbic acid on seed germination parameters and some enzymatic indices of safflower under drought stress.
Materials and Methods: The experiment was conducted as a factorial based on a completely randomized design with three replications in the Agricultural Science Laboratory of Iranshahr University in 2020. Experimental treatments included three levels of control (pretreatment with distilled water), pretreatment with gibberellic acid and ascorbic acid, and four levels of drought stress (0, -3, -6, and -9 bar). Drought stress was applied using polyethylene glycol 6000. Seed germination was carried out inside a germinator at 25 ° C for 14 days in darkness. Germination traits and enzymatic indices were measured using standard methods.
Results: The results of variance showed that most germination and growth indices of safflower seedlings decreased with increasing drought stress. Also, drought stress led to changes in the activity of antioxidant enzymes. Seed priming with gibberellic acid and ascorbic acid increased germination indices and seedling growth and improved enzymatic activity, including catalase, peroxidase, and superoxide dismutase in comparison with untreated seeds. Priming with gibberellic acid had a significant advantage. Seed priming in drought stress conditions has increased germination rate, protein content, and catalase, peroxidase, and ascorbic dismutase activity, respectively, compared to the control.
Conclusion: In general, seed priming of safflower using gibberellic acid changed the activity of antioxidant enzymes. These activities ultimately moderated the negative effects of drought stress and increased germination parameters.
Highlights:
Introduction: Safflower is a plant that has been considered due to its high medicinal and nutritional value, especially in the extraction of edible oils in developed countries. Drought is one of the most important harmful factors in arid and semi-arid regions of the world that affects plant production. Modifiers play an important role in plant adaptation to stress conditions. Among these compounds are the hormone gibberellic acid and the antioxidant ascorbic acid, which increase plant tolerance to adverse environmental conditions. The present study investigated the effect of gibberellic acid and ascorbic acid on seed germination parameters and some enzymatic indices of safflower under drought stress.
Materials and Methods: The experiment was conducted as a factorial based on a completely randomized design with three replications in the Agricultural Science Laboratory of Iranshahr University in 2020. Experimental treatments included three levels of control (pretreatment with distilled water), pretreatment with gibberellic acid and ascorbic acid, and four levels of drought stress (0, -3, -6, and -9 bar). Drought stress was applied using polyethylene glycol 6000. Seed germination was carried out inside a germinator at 25 ° C for 14 days in darkness. Germination traits and enzymatic indices were measured using standard methods.
Results: The results of variance showed that most germination and growth indices of safflower seedlings decreased with increasing drought stress. Also, drought stress led to changes in the activity of antioxidant enzymes. Seed priming with gibberellic acid and ascorbic acid increased germination indices and seedling growth and improved enzymatic activity, including catalase, peroxidase, and superoxide dismutase in comparison with untreated seeds. Priming with gibberellic acid had a significant advantage. Seed priming in drought stress conditions has increased germination rate, protein content, and catalase, peroxidase, and ascorbic dismutase activity, respectively, compared to the control.
Conclusion: In general, seed priming of safflower using gibberellic acid changed the activity of antioxidant enzymes. These activities ultimately moderated the negative effects of drought stress and increased germination parameters.
Highlights:
- The role of gibberellic acid and ascorbic acid on safflower seed germination traits was investigated.
Article number: 2
Type of Study: Research |
Subject:
General
Received: 2021/12/17 | Revised: 2024/02/21 | Accepted: 2022/04/24 | ePublished: 2023/06/14
Received: 2021/12/17 | Revised: 2024/02/21 | Accepted: 2022/04/24 | ePublished: 2023/06/14
References
1. Abdul Baki, A.A. and Anderson, J.D. 1973. Vigor determination in soybean seed by multiple criteria. Crop Science, 13: 630-633. [DOI:10.2135/cropsci1973.0011183X001300060013x]
2. Abedi, T. and Pakniat, H. 2010. Antioxidant enzyme changes in response to drought stress in ten cultivars of oilseed rape (Brassica napus L.). Czech Journal of Genetics and Plant Breeding, 46(10): 27-34. [DOI:10.17221/67/2009-CJGPB]
3. Afkari, A. 2017. Effect of seed priming on germination characteristics and some antioxidant enzymes activity of Basil (Ocimum basilicum L.) under drought stress conditions. Developmental Biology, 9(3): 33-44. [In Persian with English Summary].
4. Agrawal, S., Sairam, P.K., Srivasta, G.C. and Meena, R.C. 2005. Changes in antioxidant enzymes activity and oxidative stress by abscisic acid and salicylic acid in wheat. Biologia Plantarum, 49(4): 541-550. [DOI:10.1007/s10535-005-0048-z]
5. Alvarenga, R.O. and Marcos-Filho, J. 2014. Vigor evaluation of stored cotton seeds, including the Seed Vigor Imaging System (SVIS). Journal of Seed Science, 36: 222-230. [DOI:10.1590/2317-1545v32n2944]
6. Ansari, O., Azadi, M.S., Sharif-Zadeh, F. and Younesi, E. 2013. Effect of hormone priming on germination characteristics and enzyme activity of mountain rye (Secale montanum) seeds under drought stress conditions. Journal of Stress Physiology and Biochemistry 9(3): 61-71.
7. Ansari, O., Choghazardi, H.R., Sharif Zadeh, F. and Nazarli, H. 2012. Seed reserve utilization and seedling growth of treated seeds of mountain rye (Secale Montanum) as affected by drought stress. Cercetări Agronomice în Moldova, 2: 43-48. [DOI:10.2478/v10298-012-0013-x]
8. Arslan, B. and Kucuk, M. 2005. Oil content and fatty acid composition of some safflowers cultivars in Van (Turkey). Proceedings of the 6th International safflower Conference, Istanbul, Turkey, 6-11 June: 167-174.
9. Ashraf, M. and Harris, P. 2004. Potenial biochemical indicators of salinity tolerance in plants. Plant Science, 166: 3-16. [DOI:10.1016/j.plantsci.2003.10.024]
10. Ataiee Samaq, H., Omidi, H., Aghighi Shahverdi, M. and Mohabeli, M. 2015. The effect of gibberellic acid and abscisic acid on germination indices of medicinal plant Securigera (Securigera securidaca L.) under salinity stress. Journal of Seed Research, 5(4): 21-33. [In Persian with English Summary].
11. Bai, Y., Xiao, S., Zhang, Z., Zhang, Y., Sun, H., Zhang, K., Wang, X., Bai, Z., Li, C. and Liu, L. 2020. Melatonin improves the germination rate of cotton seeds under drought stress by opening pores in the seed coat. PeerJ Computer Science, 8: e9450. [DOI:10.7717/peerj.9450] [PMID] [PMCID]
12. Bradford, K.J. 1995. Water relation in seed germination. In: Kigel, J. and Galili, G. (eds.), Seed development and germination. Marcel Dekker, Inc, New York, 351-396. [DOI:10.1201/9780203740071-13]
13. Bradford, M. 1976. A rapid and sensitive method for the quantitation of protein utilizing the principle of protein-dye binding. Annual Review Biochemistry, 72: 248-254. [DOI:10.1016/0003-2697(76)90527-3] [PMID]
14. Chance, B. and Maehly, A.C. 1955. Assay of catalase and peroxidases. Methods in Enzymology, 2: 764-765. [DOI:10.1016/S0076-6879(55)02300-8]
15. Channaoui, S., Elkahkahi, R., Mazouz, H., Charafi, J., El-Fechtali, M. and Nabloussi, A. 2016. Study of the effect of drought stress on germination and seedlings growth of five varieties of rapeseed (Brassica napus L.). Jahorina, Bosnia and Herzegovina. Proceedings, 990-995.
16. Daneshvar, F. and Khajoei-Nejad, G. 2015. Study of bio-fertilizers application effects on yield potential and yield components of safflower (Carthamus tinctorius L.) cultivars under different irrigation regimes. Iranian of Irrigation and Water Engineering, 4: 59-69. [In Persian with English Summary].
17. De Tullio, M.C., Paciolla, C., Dalla Vecchia, F., Rascio, N., Gemerico, S., De garal Liso, R. and Arrigoni, O. 2004. Changes in onion root development induced by the inhibition of peptidyl- proly1 hydroxylase and influence of the ascorbate system on cell division and elongation. Planta, 209: 424-434. [DOI:10.1007/s004250050745] [PMID]
18. Ebrahimi, O., Esmaili, M.M., Sabori, H. and Tahmasebi, A. 2013. Effect of salinity and drought stress on germination two species of Tall Wheatgrass (Agropyron elongatum, Agropyron desertrum). Desert Ecosystem Engineering Journal, 1(1): 31-38. [In Persian with English Summary].
19. Elouaer, M.A. and Hannachi, C. 2012. Seed priming to improve germination and seedling growth of safflower (Carthamus tinctorius L.) under salt stress. Eurasian Journal of Bio Sciences, 6(1): 76-84. [DOI:10.5053/ejobios.2012.6.0.9]
20. Erdal, S. and Dumlupinar, R. 2011. Mammalian sex hormones stimulate antioxidant system and enhance growth of chickpea plants. Acta Physiologiae Plantarum, 33(3): 1011-1017. [DOI:10.1007/s11738-010-0634-3]
21. Fathi-Amirkhiz, K., Amini, M., Modares-Sanavi, A.M., Reza-Zadeh, A. and Heshmati, S. 2011. Effect of iron application on enzymic activity, grain yield and oil content of Safflower under water deficit conditions. Iranian Journal of Crop Sciences, 13(3): 452-465. [In Persian with English Summary].
22. Ghaderi, N., Talaei, A., Ebadi, A. and Lesani, H. 2010. Study of some physiological characteristics in sahani', 'bidane-sefid' and 'farkhii' grapes during drought stress and their subsequent recovery. Iranian Journal of Horticultural Sciences, 41(2): 179-188. [In Persian with English Summary].
23. Ghanati, F. and Rahmati, I.M. 2006. Improvement of antioxidant system and decrease of lignin by nickel treatment in tea plant. Journal of Plant Nutrition, 29: 1649-1661. [DOI:10.1080/01904160600851536]
24. Ghanati, F., Morita, A. and Yokota, H. 2002. Induction of suberin and increase of lignin content by excess Boron in Tabacco cell. Soil Science and Plant Nutrition, 48(3): 357-364. [DOI:10.1080/00380768.2002.10409212]
25. Gornik, k., Grzesik, M. and Duda, B.R. 2008. The effect of chitosan on rooting of grapevine cuttings and on subsequent plant growth under drought and temperature stress. Journal of Fruit and Ornamental Plant Research, 16: 333-343.
26. Haqjoo, M. and Bahrani, A. 2018. The effect of Salicylic and Gibberellic acid pretreatments on the accumulation of some ions and germination indices in canola under salinity stress, Journal of Plant Production Science, 8(1): 23-34. [In Persian with English Summary].
27. Hsu, J. and Sung, J. 1997. Antioxidant role of glutathione associated with accelerated aging and hydration of triploid watermelon seeds. Physiologia Plantarum, 100: 967-974. [DOI:10.1034/j.1399-3054.1997.1000424.x]
28. Ibrahim, M, Zeid, N. and El-semary, A. 2001. Response of two differentially drought tolerant varieties of maize to drought stress. Pakistan Journal of Biological Sciences, 4(7): 779-784. [DOI:10.3923/pjbs.2001.779.784]
29. International Seed Testing Association. 2010. International rules for seed testing. Seed Science and Technology. The Seed Technologist Newsletter.
30. Jahanbakhsh, S., Parmoon, GH. and Joudi, Z. 2018. Effect drought and salt stress on germination, establishment and antioxidant enzyme activity different ecotypes chamomile (Matricaria chamomilla L). Journal of Plant Physiology, 8(30): 1-19.
31. Janda, T., Szalai, G., Tari, I. and Paldi, E. 1999. Hydroponic treatment with salicylic acid decreases the effects of chilling injury in maize (Zea mays L.) plants. Journal Plants, 208: 175-180. [DOI:10.1007/s004250050547]
32. Kaya, C., Tuna, A.L. and Yokas, I. 2009. The role of plant hormones in plants under salinity stress. In: Ashraf, M., Ozturk, M. and Athar, HR. (eds.). Salinity and water stress: improving crop efficiency. Springer, Berlin.
33. Kaya, M.D., Okçu, G., Atak, M., Cıkılı, Y. and Kolsarıcı, O. 2006. Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). European Journal of Agronomy, 24(4): 291-295. [DOI:10.1016/j.eja.2005.08.001]
34. Lari Yazdi, H., Amiri, H. and Lak, R. 2009. Study of interaction effects between gibberellic acid and ascorbic acid on germination percentage and rate on two cultivars of rapeseed (Brassica napus L.) (RGS & Hayola401) in different concentrations of NaCl. Journal of Biology, 2(4): 45-50. [In Persian with English Summary].
35. Lawlor, D.W. 2002. Limitation to photosynthesis in water stressed leaves: stomata vs. metabolism and the role of ATP. Annals of Botany, 89(7): 871-885. [DOI:10.1093/aob/mcf110] [PMID] [PMCID]
36. Li, J., Yin, L., Jongsma, M. and Wang, C. 2011. Effects of light, hydropriming and abiotic stress on seed germination, and shoot and root growth of pyrethrum (Tanacetum cinerariifolium). Industrial Crops and Products, 34: 1543-1549. [DOI:10.1016/j.indcrop.2011.05.012]
37. Liu, J., Hasanuzzaman, M., Wen, H., Zhang, J., Peng, T., Sun, H. and Zhao, Q. 2019. 'High temperature and drought stress cause abscisic acid and reactive oxygen species accumulation and suppress seed germination growth in rice'. Protoplasma, 256: 1217-1227. [DOI:10.1007/s00709-019-01354-6] [PMID]
38. Lou, D., Wang, H., Liang, G. and Yu, D. 2017. OsSAPK2 confers abscisic acid sensitivity and tolerance to drought stress in rice. Frontiers in Plant Science, 8: 993. [DOI:10.3389/fpls.2017.00993] [PMID] [PMCID]
39. Mahdavi, B., Modarres Sanavy, S.A.M., Aghaalikhani, M. and Sharifi, M. 2014. Effect of chitosan on safflower (Carthamus tinctorius L.) Seed germination and antioxidant enzymes activity under water stress. Journal of Plant Research, 26: 352-365. [In Persian with English Summary].
40. Michel, B.E. and Kaufman, M.R. 1973. The osmotic potential of polyethylene glycol 6000. Plant Physiology, 51: 914-916. [DOI:10.1104/pp.51.5.914] [PMID] [PMCID]
41. Moori, S., Eisvand, H.R., Ismaili, A. and Sasani, Sh. 2018. Effects of seed preparation with gibberellic acid and brassinosteroid on germination indices and physiological traits after accelerated aging. Iranian Journal of Seed Science and Technology, 7(2): 183-193. [In Persian, with English Summary].
42. Moradi, A., Sharifzadeh, F., Tavakol Afshari, R. and Ma'ali Amiri, R. 2010. Effect of pretreatment on germination and seedling growth of tall wheatgrass under optimal moisture and drought stress conditions. Journal of Range Research, 4(3): 462-473. [In Persian, with English Summary].
43. Moradian, Z., Omidi, H., Karimi, T., Azadbakht, F. and Bazkani, R. 2017. The effect of hormonal pretreatment on germination and seedling growth indices of urban Balengo (Lallemantia iberica Fisch. & C.A. Mey) under drought stress. Seed Research, 7: 21-29.
44. Naderi Zarnaghi, R., Valizadeh, M. and Fotovat, R. 2014. Electrophoretic analysis of antioxidant enzymes activity under drought stress in winter wheat genotypes at tillering stage. Cereal Research, 4(3): 185 -197. [In Persian with English Summary].
45. Nakano, Y. and Asada, K. 1981. Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant and Cell Physiology, 22: 867-880.
46. Nelson, C.P. 2000. Water potential: The key to successful seed priming. Decagon Devices, Inc. AN4101- 10.
47. Ostadian Bidgoli, R., Balouchi, H., Soltani, E. and Moradi, A. 2017. Effects of temperature and water potential on seed germination characteristics in safflower (Carthamus Tinctorius L.) sofeh var. Iranian Journal of Seed Science and Technology, 6(1): 11-22. [In Persian with English Summary].
48. Pagter, M., Bragato, C., Malagoli, M. and Brix, H. 2009. Osmotic and ionic effects of NaCl and Na2SO4 salinity on Phragmites australis. Aquatic Botany, 90: 43-51. [DOI:10.1016/j.aquabot.2008.05.005]
49. Patade, V.Y., Maya, K. and Zakwan, A. 2011. Seed priming mediated germination improvement and tolerance to subsequent exposure to cold and salt stress in capsicum. Research Journal of Seed Science, 4(3): 125-136. [DOI:10.3923/rjss.2011.125.136]
50. Rady, M.M., Boriek, S.H., El-Mageed, A., Taia, A., Seif El-Yazal, M.A., Ali, E.F., Hassan, F.A. and Abdelkhalik, A. 2021. Exogenous gibberellic acid or dilute bee honey boosts drought stress tolerance in Vicia faba by rebalancing osmoprotectants, antioxidants, nutrients, and phytohormones. Journal Plants, 10: 748. [DOI:10.3390/plants10040748] [PMID] [PMCID]
51. Rafatpour, S., Shahriari, A., Saberi, M., Karvarinasab, M. and Tarnian, F. 2020. 'Effects of priming on germination and seedling growth of zygophyllum atriplicoides under drought stress'. Journal Ecopersia, 8: 89-96. [In Persian with English Summary].
52. Rahimi, A. 2013. Seed priming improves the germination performance of cumin (Cuminum syminum L.) under temperature and water stress. Industrial Crops and Products, 42: 454- 460. [DOI:10.1016/j.indcrop.2012.06.026]
53. Sairam, R.K., Desmukh, P.S. and Saxena, D.C. 1998. Role of antioxidant systems in wheat genotypes tolerant to water stress. Biologia Plantarum, 41: 387-394. [DOI:10.1023/A:1001898310321]
54. Scott, S.J., Jones, R.A. and Williams, W.A. 1984. Review of data analysis methods for seed germination. Journal Crop Science, 24: 1192-1199. [DOI:10.2135/cropsci1984.0011183X002400060043x]
55. Seyed sharif, R. and Seyed sharif. R. 2008. Evaluation the effects of polyethylene glycol on germination and growth seedling carthamus cultivars. Journal of Biology, 21(3): 400-410. [In Persian with English Summary].
56. Sharma, A. and Zheng, B. 2019. Melatonin mediated regulation of drought stress: physiological and molecular aspects. Journal Plants, 8(7):190. [DOI:10.3390/plants8070190] [PMID] [PMCID]
57. Shatpathy, P., Kar, M., Dwibedi, S.K., and Dash, A. 2018. Seed priming with salicylic acid improves germination and seedling growth of rice (Oryza sativa L.) under PEG-6000 induced water stress. International Journal of Current Microbiology and Applied Science, 7: 907-924. [DOI:10.20546/ijcmas.2018.710.101]
58. Soltani, A., Galeshi, S., Zeinali, E. and Latifi, N. 2001. Genetic variation for and interrelationships among seed vigor traits in wheat from the Caspian Sea coasts of Iran. Seed Science and Technology, 29: 653-662.
59. Tuna, A.L., Kaya, C., Dikilitas, M. and Higgs, D. 2008. The combined effects of gibberellic acid and salinity on some antioxidant enzyme activities, plant growth parameters and nutritional status in maize plants. Environmental and Experimental Botany, 62: 1-9. [DOI:10.1016/j.envexpbot.2007.06.007]
60. Vyas, D. and Kumar, S. 2005. Purification and partial characterization of a low temperature responsive Mn-SOD from tea (Camellia sinensis L.). Biochemical and Biophysical and Research Communications, 329(3): 831-838. [DOI:10.1016/j.bbrc.2005.02.051] [PMID]
61. Yazdan Bioki, R., Rezvani Moghad'dam, P., Kouchaki, A., Behzad Amiri, M., Falahi, J. and Deyhim Fard, R. 2010. Effect of different nutrition of wheat (cv. Sayonez) on its germination indices and seedling growth under drought stress and biological muck. Journal of Agroecology, 2(2): 266-276. [In Persian, with English Summary].
62. Zabarjadi, A., Soheilikhah, J., Ghasempour, H.R. and Visipour, A. 2012. Effect of drought-induced stress by peg6000 on physiological and morphological traits of safflower (Carthamus tinctorius L.) Seed Germination in Order to Selection of Drought Tolerant Genotypes. Iranian Journal of Biology, 25(2): 252-263. [In Persian, with English Summary].
63. Zare, S., Tavili, A., Shahbazi, A. and Riyahi, A. 2010. The effect of different salicylic acid concentrations on improved germination characteristics of Salad burnet (Sanguisorba minor L.) under salt and drought stress. Journal of Range and Watershed Management, 63: 29-40.
64. Zhang, N., Zhao, B., Zhang, H.J., Weeda, S., Yang, C., Yang, Z.C., Ren, S. and Guo, Y.D. 2012. Melatonin promotes water stress tolerance, lateral root formation, and seed germination in cucumber (Cucumis sativus L.). Journal of Pineal Research, 54: 15-23. [DOI:10.1111/j.1600-079X.2012.01015.x] [PMID]
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