Volume 9, Issue 1 ((Spring and Summer) 2022)                   Iranian J. Seed Res. 2022, 9(1): 127-146 | Back to browse issues page


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Malek M, Hassani F, Rezvani Khorshidi E, Shayanfar A, Oskoee B, Dehshiri A. (2022). Dormancy and germination response of Galbanum seeds (Ferula gummosa) under different hormonal pre-treatments and cold stratification. Iranian J. Seed Res.. 9(1), : 8 doi:10.52547/yujs.9.1.127
URL: http://yujs.yu.ac.ir/jisr/article-1-534-en.html
Assistant Professor, Seed and Plant Certification and Registration Research Institute, Agricultural Research, Education and Extension Organization (AREEO), Karaj, Iran , F.hassani@areo.ac.ir
Abstract:   (1656 Views)
Extended Abstract
Introduction: Galbanum is one of the most important native medicinal plants in Iran, and nowadays the use of its products and derivatives has become widespread in various industries. Thus, the excessive harvest of this species within its natural habitats has increased. Therefore, the species is enlisted as prone to extinction. Due to the lack of information about germination behavior and dormancy alleviating or breaking methods of Galbanum seeds, less attention has been paid to this plant species by seed science researchers. Hence, this study was designed and performed to investigate the effects of different treatments on Galbanum seed dormancy-breaking.
Materials and Methods: In this study, two Galbanum seed populations were collected from Boyer-Ahmad pastures (Iran, Kohgiluyeh and Boyer-Ahmad Province) and Pakan Bazr Isfahan Company (Iran). Seeds were exposed to a cold stratification test for 120 days and germinability and dormancy breaking responses were studied. Also, seed drying effects on germinability after exposure to stratification treatment were investigated to evaluate the practical aspects of this treatment. The impacts of different hormonal pre-treatments including gibberellic acid (GA3) and cytokines (Kinetin, 6-Benzylaminopurine) at different concentrations on germination and dormancy behaviors were investigated so as to assess the effect of these treatments on seed dormancy breaking.
Results: Results showed that germination/dormancy behaviors of Galbanum populations were different and also the effect of different treatments on seeds germination and dormancy varied. An increase in germination was observed when stratification was applied for up to 60 days on two seed populations, thereafter germination decreased by prolonged stratification. Also, it was revealed that desiccation of the treated seeds after stratification reduced seed germination. Examining the cause of this issue revealed that the most important reason for the decrease in seed germination in long periods of stratification was the germination of seeds in the stratification bed and in other words, the difference in seed dormancy levels in different stratification periods. Seed germination behavior and dormancy breaking were improved significantly in both Galbanum populations when the different concentration of gibberellic acid and cytokinins was used.
Conclusion: The results of this study indicated the existence of different levels of seed dormancy in different Galbanum populations. It was also found that cold stratification and hormonal pre-treatment of seeds can be significantly effective in seed dormancy breaking. Eventually, it was concluded that Galbanum seed dormancy was classified as semi-deep and deep morphological dormancy due to the effects of different treatments.

Highlights:
  1. The effect of cold stratification treatments over long periods was investigated on Galbanum seed germination behaviors for the first time.
  2. The desiccation of treated seeds under different cold stratification periods was studied on the germinability of Galbanum seeds for the first time.
  3. AUC (Area under the curve) index was introduced as a practical index in seed dormancy breaking comparison for the first time in domestic research.
Article number: 8
Full-Text [PDF 521 kb]   (696 Downloads)    
Type of Study: Research | Subject: Seed Physiology
Received: 2021/08/24 | Revised: 2024/02/21 | Accepted: 2022/02/19 | ePublished: 2022/12/11

References
1. Aihua, L., Shunyuan, J., Guang, Y., Ying, L., Na, G., Tong, C., Liping, K. and Luqi, H. 2018. Molecular mechanism of seed dormancy release induced by fluridone compared with cold stratification in Notopterygium incisum. BMC Plant Biology, 18(1): 1-16. [DOI:10.1186/s12870-018-1333-2] [PMID] [PMCID]
2. Bagheri, S.M., Mohamadsadeghi, H. and Hejazian, E.S. 2017. Antinociceptive effect of seed's essential oil of Ferula assa-foetida in mice. International Journal of Clinical and Experimental Physiology, 4(1): 34-37. [DOI:10.4103/ijcep.ijcep_5_17]
3. Baskin, C.C. and Baskin, J.M. 2014. Seeds ecology, biogeography, and evolution of dormancy and germination. Second edition. San Diego, CA: Academic Press.
4. Baskin, J.M. and Baskin, C.C. 2004. A classification system for seed dormancy. Seed Science Research, 14: 1-16. [DOI:10.1079/SSR2003150]
5. Bazyar, S., Najafi, H. and oveysi, M. 2015. Differences in germination of Datura weed seed mass influenced by different climatic conditions during different seasons. Journal of Weed Research, 7: 43-51. [In Persian with English Summary].
6. Bewley, J.D, Black, M. and Halmer, P. 2006. The encyclopedia of seeds: science, technology and uses. Wallingford, UK: CAB International.
7. Bewley, J.D. and Black, M. 2012. Physiology and biochemistry of seeds in relation to germination: volume 2: viability, dormancy, and environmental control. Springer Science & Business Media. 306-312.
8. Bewley, J.D., Bradford, K.J. Hilhorst, H.W.M. and Nonagaki, H. 2013. Seeds: physiology of development, germination and dormancy, 3th Edition. Springer. New York Heidelberg Dordrecht London, Switzerland. 392p.
9. Butler, L.H., Hay, F.R., Ellis, R.H., Smith, R.D. and Murray, T.B. 2009. Priming and re-drying improve the survival of mature seeds of Digitalis purpurea during storage. Annals of Botany, 103: 1261-1270. [DOI:10.1093/aob/mcp059] [PMID] [PMCID]
10. Chen, S.Y., Chou, S.H., Tsai, C.C., Hsu, W.Y., Baskin, C.C., Baskin, J.M., and Kuo-Huang, L.L. 2015. Effects of moist cold stratification on germination, plant growth regulators, metabolites and embryo ultrastructure in seeds of Acer morrisonense (Sapindaceae). Plant Physiology and Biochemistry, 94: 165-173. [DOI:10.1016/j.plaphy.2015.06.004] [PMID]
11. El-Kassaby, Y. A., Moss, I., Kolotelo, D. and Stoehr, M. 2008. Seed germination: mathematical representation and parameters extraction. Forest Science, 54(2): 220-227.
12. Finch-Savage, W.E. and Leubner-Metzger, G. 2006. Seed dormancy and the control of germination. New Phytologist, 171(3): 501-523. [DOI:10.1111/j.1469-8137.2006.01787.x] [PMID]
13. Geshnizjani, N., Ghaderi-Far, F., Willems, L.A., Hilhorst, H.W. and Ligterink, W. 2018. Characterization of and genetic variation for tomato seed thermo-inhibition and thermo-dormancy. BMC Plant Biology, 18: 1-12. [DOI:10.1186/s12870-018-1455-6] [PMID] [PMCID]
14. Ghaemizadeh, F., Dashti, F. and Ghahremani-majd, H. 2011. Study of dormancy and seed germination in different populations of shallots, 7th Iranian Congress of Horticultural Sciences, Isfahan. [In Persian with English Summary].
15. Hassani, S. B., Saboora, A., Radjabian, T. and Fallah Husseini, H. 2009. Effects of temperature, GA3 and Cytokinins on breaking seed dormancy of Ferula assa-foetida L. Iranian Journal of Science and Technology, 33(1): 75-85.
16. Hutchison, C.E. and Kieber, J.J. 2002. Cytokinin signaling in Arabidopsis. The Plant Cell, 14: 47-59. [DOI:10.1105/tpc.010444] [PMID] [PMCID]
17. Joosen, R.V., Kodde, J., Willems, L.A., Ligterink, W., Van der Plas, L.H. and Hilhorst, H.W. 2010. GERMINATOR: a software package for high‐throughput scoring and curve fitting of Arabidopsis seed germination. The Plant Journal, 62(1): 148-159. [DOI:10.1111/j.1365-313X.2009.04116.x] [PMID]
18. Kakimoto, T. 2003. Biosynthesis of cytokinins. Journal of Plant Research, 116(3): 233-239. [DOI:10.1007/s10265-003-0095-5] [PMID]
19. Karssen, C.M., Zagorski, S., Kepczynski, J. and Groot, S. 1989. Key role for endogenous gibberellins in the control of seed germination. Annals of Botany, 63(1): 71-80. [DOI:10.1093/oxfordjournals.aob.a087730]
20. Keshtkar, H.R., Azarnivand, H., Etemad, V. and Moosavi, S.S. 2008. Seed dormancy-breaking and germination requirements of Ferula ovina and Ferula gummosa. Desert, 13(1): 45-51.
21. Khan, A.A. 1971. Cytokinins: permissive role in seed germination. Science, 171: 853-859. [DOI:10.1126/science.171.3974.853] [PMID]
22. Khan, A.A. 1994. Induction of dormancy in non-dormant seeds. Journal of the American Society for Horticultural Science, 119(3): 408-413. [DOI:10.21273/JASHS.119.3.408]
23. Koster, K.L. and Leopold, A.C. 1988. Sugars and desiccation tolerance in seeds. Plant physiology, 88(3): 829-832. [DOI:10.1104/pp.88.3.829] [PMID] [PMCID]
24. Labbafi-hoseinabadi, M., Mehrafarin, A., Naghdiabadi, H., Tavakkoli, M. and Ghorbani-nohuji, M. 2018. Evaluation of the effect of different chemical and non-chemical treatments on seed dormancy failure of Ferula gummosa Boiss.. Ecophytochemistry of Medicinal Plants, 6: 80-88. [In Persian with English Summary].
25. Malek, M., Hamidi, S., Ghaderi-Far, F., Gorzin, M., Pahlavani, M.H. and Esmaeilzadeh Moghaddam, M. 2020. Quantification of temperature effects on germination and induction of secondary dormancy in barley cultivars. Environmental Stresses in Crop Sciences, 13: 995-1007. [In Persian with English Summary]
26. Mozaffarian, V. 2004. Dictionary of Iranian Plants. Contemporary culture. Tehran, pp. 228-229. [In Persian].
27. Nasiri, M., Yazdanbakhsh, N. and Falahati Anbaran, M. 2016. Investigation of the effect of chilling duration on seed germination of different populations of Heracleum persicum. Iran, 19th National Congress and 7th International Congress of Iranian Biology, Tabriz. [In Persian with English Summary].
28. Paparella, S., Araújo, S.S., Rossi, G., Wijayasinghe, M., Carbonera, D. and Balestrazzi, A. 2015. Seed priming: state of the art and new perspectives. Plant Cell Reports, 34(8): 1281-1293. [DOI:10.1007/s00299-015-1784-y] [PMID]
29. Parsa, S., Ahmadi, K., Gazanchian, A. and Mahmoodi, S. 2016. Effect of chilling medium and dormancy breaking treatments on seed germination of galbanum (Ferula gummosa Boiss.). Journal of Seed Ecophysiology, 1: 201-215. [In Persian with English Summary].
30. Pouresmaeil, M. and Sharifi, M. 2003. Dormancy-Breaking in Bunium Persicum seeds by stratification and some cytokinins. Iranian Journal of Medicinal and Aromatic Plants Research, 19: 183-193. [In Persian with English Summary].
31. Powell, A.A., Yule, L.J., Jing, H.C., Groot, S.P., Bino, R.J. and Pritchard, H.W. 2000. The influence of aerated hydration seed treatment on seed longevity as assessed by the viability equations. Journal of Experimental Botany, 5: 2031-2043. [DOI:10.1093/jexbot/51.353.2031] [PMID]
32. Rahnama-Ghahfarokhi, A. and Tavakkol-Afshari, R. 2007. Methods for dormancy breaking and germination of galbanum seeds (Ferula gummosa). Asian Journal of Plant Sciences, 6(4): 611-616. [DOI:10.3923/ajps.2007.611.616]
33. Rostami, M. and Tavakkol-Afshari, R. 2014. Determining the type of dormancy of Galbanum seeds of Damavand mass (Ferula gummosa Bioss.) And determining the seed requirements for dormancy breaking. Journal of Crop Science, 2: 255-263. [In Persian]
34. Rouhi, H.R., Rahmati, H., Saman, M., Shahbodaghloo, A.R., Karimi, F.A., Moosavi, S.A. and Karimi, F. 2012. The effects of different treatments on dormancy-breaking of Galbanum seeds (Ferula gummosa Boiss.). International Journal of AgriScience, 2(7): 598-604.
35. Salar, N.A., EZ, A.H. and Taherian, K. 2002. A survey on cultivation and propagation methods of Ferula gummosa in Semnan Province.
36. Sharifi, H., Khajeh-Hosseini, M. and Rashed-Mohassel, M. H. 2015. Study of seed dormancy in seven medicinal species from Apiaceae. Iranian Journal of Seed Research, 2 (1): 25-36
37. Sharifi, H., Nemati, A. and Gerdakaneh, M. 2017. Breaking seed dormancy and improve germination of four medicinal species of Apiaceae by gibberellic acid and prechilling treatments. Iranian Journal of Seed Science and Research, 4: 27-38. [In Persian with English Summary]
38. Sharifi, M., and Pouresmael, M., 2006. Breaking seed dormancy in Bunium persicum by stratification and chemical substances. Asian Journal of Plant Sciences, 5: 695-699 [DOI:10.3923/ajps.2006.695.699]
39. Shayanfar, A., Ghaderi-Far, F., Behmaram, R., Soltani, A. and Sadeghipour, H. R. 2018. Assessment of germination and secondary dormancy behaviours of lines and cultivars of canola. Journal of Crops Improvement, 19(4). [In Persian with English Summary]
40. Soltani, E., Baskin, C.C., Baskin, J.M., Soltani, A., Galeshi, S., Ghaderi-far, F. and Zeinali, E. 2016. A quantitative analysis of seed dormancy and germination in the winter annual weed Sinapis arvensis (Brassicaceae). Botany, 94: 289-300. [DOI:10.1139/cjb-2015-0166]
41. Wang, Y., Li, L., Ye, T., Zhao, S., Liu, Z., Feng, Y. Q., and Wu, Y. 2011. Cytokinin antagonizes ABA suppression to seed germination of Arabidopsis by downregulating ABI5 expression. The Plant Journal, 68(2): 249-261. [DOI:10.1111/j.1365-313X.2011.04683.x] [PMID]
42. Werner, T., and Schmülling, T. 2009. Cytokinin action in plant development. Current Opinion in Plant Biology, 12(5): 527-538. [DOI:10.1016/j.pbi.2009.07.002] [PMID]
43. Zardari, S. 2020. Factors affecting seed dormancy breakage in two medicinal plants; Giant Fennel (Ferula ovina Boiss.) and Galbanum (Ferula gummosa Boiss.). Ph.D. thesis. Gorgan University of Agricultural Sciences and Natural Resources, Iran. [In Persian with English Summary]
44. Zardari, S., Ghaderi‐Far, F., Sadeghipour, H. R., Zeinali, E., Soltani, E., and Baskin, C.C. 2019. Deep and intermediate complex morphophysiological dormancy in seeds of Ferula gummosa (Apiaceae). Plant Species Biology, 34(3): 85-94. [DOI:10.1111/1442-1984.12238]

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Iranian Journal of Seed Research

Designed & Developed by : Yektaweb


This work is licensed under a Creative Commons Attribution 4.0 International License.