Volume 8, Issue 2 ((Autumn & Winter) 2022)                   Iranian J. Seed Res. 2022, 8(2): 21-40 | Back to browse issues page

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latifzadeh Shahkhali M, Ehtehsami S M R, Moradi F. (2022). Investigating the Effects of Natural and Artificial Seed Deterioration on Reactive Oxygen Species, Antioxidant Enzymes, and Seed Germination Characteristics in Local and Improved Rice (Oryza sativa) Cultivars Derived from the Farms in Guilan Province. Iranian J. Seed Res.. 8(2), : 2 doi:10.52547/yujs.8.2.21
URL: http://yujs.yu.ac.ir/jisr/article-1-494-en.html
Associated Professor, Department of Agronomy and Plant Breeding, Faculty of Agriculture, University of Guilan, Guilan, Iran , smrehteshami@yahoo.com
Abstract:   (2950 Views)
Extended abstract
Introduction: Seed deterioration is associated with oxidative stress and uncontrolled accumulation of reactive oxygen species. Seeds have a group of enzymatic and non-enzymatic antioxidants that protect them against reactive oxygen species and help maintain seed vigor and support seed germination processes. The response of different cultivars to seed deterioration is different. Knowledge about the sources and mechanisms of deterioration and how different cultivars respond can help to select the appropriate cultivar for the region and also provide useful information in selecting and applying appropriate management methods for storage and increasing the storage time of seeds.
Materials and Methods: The Experiment was conducted as factorial based on a completely randomized block design with three replications. Treatments included one and six months of natural storage (4˚C, 11 percent moisture content of seeds and 60% RH) and accelerated aging (96 hours at 45˚C and 100% RH) applied on four cultivars including Hashemi, Gilaneh, Khazar and Domsiyah. Germination percentage, germination rate, shoot length, radicle length, shoot dry weight, radicle dry weight, shoot vigor index (SVI), α-amylase activity, catalase (CAT), peroxidase (POX), superoxide dismutase (SOD) activity, malondialdehyde (MDA) and hydrogen peroxide (H2O2) content were evaluated.
Results: The results showed that accelerated ageing and storage of seeds for six months led to reduced germination percentage, germination rate, shoot length, radicle length, shoot dry weight, radicle dry weight, shoot vigor index (SVI), α-amylase activity reduced in all four cultivars. This decrease was accompanied by an increase in the free radical content of MDA and H2O2 and a decrease in the activity of the antioxidant enzymes SOD and POX. Malondialdehyde content was lower in the seeds stored for six months. The activity of CAT increased after deterioration treatment, this increase was more intense in the seeds stored for six months. These seeds also showed lower H2O2 content compared to accelerated aging seeds. Khazar cultivar showed lower H2O2 content as a result of higher CAT enzyme activity. Moreover, this cultivar showed better germination percentage and germination rate after deterioration treatment compared to other cultivars. The decrease in germination percentage due to deterioration in Domsiyah was very severe. This cultivar showed the lowest germination percentage, germination rate, seed vigor and α-amylase activity in the accelerated aging treatment. Gilaneh and Hashemi cultivars showed higher germinability, α-amylase activity and SOD and POX at the beginning of the experiment, but after deterioration treatment, their germinability decreased along with the activity of antioxidant enzymes.
Conclusions: In all studied cultivars, the accelerated aging and storage of seeds for six months adversely affected germination rate. This decrease was accompanied by an increase in free radicals in the seeds and a decrease in the activity of antioxidant enzymes SOD and POX, which were less severe in Khazar cultivar and more severe in Domsiyah cultivar. These results indicate the greater importance of the storage conditions in Domsiyah cultivar.

1- The effect of storage and seed deterioration on the activity of antioxidant enzymes, reactive oxygen species and seed germination in rice was investigated.
2- The results of accelerated aging test and natural aging were compared for a better conclusion about the response of the cultivars.
3- The two improved cultivars were compared with two local cultivars (with high cultivation area).
Article number: 2
Full-Text [PDF 572 kb]   (680 Downloads)    
Type of Study: Research | Subject: Seed Physiology
Received: 2020/08/2 | Accepted: 2020/10/27

1. Abdul-Baki, A.A. and Anderson, J.D. 1973. Vigor determination in soybean seed by multiple criteria. Journal of Crop Science, 13(6): 630-633. [DOI:10.2135/cropsci1973.0011183X001300060013x]
2. Abeles, F.B. and Biles, C.L. 1991. Characterization of peroxidases in lignifying peach fruit endocarp. Plant Physiology, 95: 269-273. [DOI:10.1104/pp.95.1.269] [PMID] [PMCID]
3. Aebi, H. 1984. Catalase in vitro. Methods in Enzymology, 105: 121-126. [DOI:10.1016/S0076-6879(84)05016-3]
4. Bailly, C., El-Maarouf-Bouteau, H. and Corbineau, F. 2008. From intracellular signaling networks to cell death: the dual role of reactive oxygen species in seed physiology. Comptes Rendus Biologies, 331(10): 806-814. [DOI:10.1016/j.crvi.2008.07.022] [PMID]
5. Barreto, L.C. and Garcia, Q.S. 2017. Accelerated ageing and subsequent imbibition affect seed viability and the efficiency of antioxidant system in macaw palm seeds. Acta Physiologiae Plantarum, 39(3): 72-78. [DOI:10.1007/s11738-017-2367-z]
6. Bijanzadeh, E., Naderi, R., Nosrati, K. and Egan, T.P. 2017. Effects of accelerated ageing on germination and biochemistry of eight rice cultivars. Journal of Plant Nutrition, 40(2): 156-164. [DOI:10.1080/01904167.2016.1201502]
7. Brar, N.S., Kaushik, P. and Dudi, B.S. 2019. Assessment of natural ageing related physio-biochemical changes in onion seed. Agriculture, 9(8): 1-15. [DOI:10.3390/agriculture9080163]
8. Cao, D., Chen, S., Huang, Y., Qin, Y. and Ruan, G. 2019. Effects of artificial aging on physiological characteristics of rice seeds with different dormancy characteristics. Agricultural Biotechnology, 8(1): 52-56.
9. Chandel, R K., Khan, Z.and Gandotra, S. 2015. Alterations in protein and isozymes profiles during accelerated ageing in soybean (Glycine Max (L.) Merrill). Journal of Functional and Environmental Botany, 5(1): 64-69. [DOI:10.5958/2231-1750.2015.00010.4]
10. Chauhan, D.S., Deswal, D.P., Dahiya, O.S. and Punia, R.C. 2011. Change in storage enzymes activities in natural and accelerated aged seed of wheat (Triticum aestivum). Indian Journal of Agricultural Sciences, 81(11): 1037-1040.
11. Chen, D., Li. Y., Fang, T. Shi, X. and Chen, X. 2016. Specific roles of tocopherols and tocotrienols in seed longevity andgermination tolerance to abiotic stress in transgenic rice. Plant Science, 244: 31-39. [DOI:10.1016/j.plantsci.2015.12.005] [PMID]
12. Copeland, L. and MaDonald, M.B. 2008. Principles of Seed Science and Technology. (Translated: Akram Ghaderi, F., Kamkar, B. and Soltani, A). Ferdowsi University of Mashhad Publication. 511p. [In Persian].
13. Dantas, A.F., Fascineli, M.L., Jose, S.C.B.R., Padua, J.G., Gimenes M.A. and Grisolia, C.K. 2019. Loss of genetic integrity in artificially aged seed lots of rice (Oryza sativa L.) and common bean (Phaseolus vulgaris L.). Mutation Research/Genetic Toxicology and Environmental Mutagenesis, 846: 1-6. [DOI:10.1016/j.mrgentox.2019.07.008] [PMID]
14. Dey, G., Bhupinder, S. and Banerjee, R. 2003. Immobilization of alpha-amylase produced by Bacillus circulans GRS 313. Brazilian Archives of Biology and Technology, 46(2): 167-176. [DOI:10.1590/S1516-89132003000200005]
15. Fu, H., Cao, D.D., Hu, W.M., Guan, Y.J., Fu, Y.Y., Fang, Y.F. and Hu, J. 2017. Studies on optimum harvest time for hybrid rice seed. Journal of the Science of Food and Agriculture, 97(4): 1124-1133. [DOI:10.1002/jsfa.7838] [PMID]
16. Gao, J., Fu, H., Zhou, X., Chen, Z., Luo, Y., Cui, B., Chen, G. and Liu, J. 2016. Comparative proteomic analysis of seed embryo proteins associated with seed storability in rice (Oryza sativa L.) during natural aging. Plant Physiology and Biochemistry, 103: 31-44. [DOI:10.1016/j.plaphy.2016.02.026] [PMID]
17. Ghasemi, E., Goodarzian Ghahfarokhi, M., Darvishi, B. and Heidari kazafi, Z. 2014. The effect of hydro-priming on germination on characteristics, seedling growth and antioxidant activity of accelerated aging wheat seeds. Cercetari Agronomice in Moldova, 4: 41-48. [DOI:10.1515/cerce-2015-0003]
18. Ghassemi-Golezani, K., Khomari, S., Dalil, B., Hosseinzadeh-Mahootchy, A. and Chadordooz-Jeddi, A. 2010. Effects of seed aging on field performance of winter oilseed rape. Journal of Food, Agriculture and Environment, 8(1): 175-178.
19. Giannopolitilis, C.N. and Ries, S.K. 1997. Superoxide dismutase.o.purification and quantitative relationship with water soluble protein in seedlings. Journal of Plant Physiology, 59: 315-318. [DOI:10.1104/pp.59.2.315] [PMID] [PMCID]
20. Goel, A., Goel, A.K. and Sheoran, I.S. 2003. Changes in oxidative stress enzymes during artificial ageing in cotton (Gossypium hirsutum L.) seeds. Journal of Plant Physiology, 160: 1093-1100. [DOI:10.1078/0176-1617-00881] [PMID]
21. Goodarzian Ghahfarokhi, M., Ghasemi, E., Saeidi, M. and Heidari Kazafi, Z. 2014. The effect of accelerated aging on germination characteristics, seed reserve utilization and malondialdehyde content of two wheatcultiars. Journal of Stress Physiology and Biochemistry, 10(2): 15-23.
22. Heath, R.L. and Packer, L. 1968. Photoperoxidation in isolated chloroplasts: I. Kinetics and stoichiometry of fatty acid peroxidation. Archives of Biochemistry and Biophysics, 125(1): 189-198. [DOI:10.1016/0003-9861(68)90654-1]
23. Kapoor, N., Arya, A., Siddiqui, M.A., Amir, A. and Kumar, H. 2010. Seed deterioration in chickpea (Cicer arietinum L.) under accelerated aging. Asian Journal of Plant Sciences, 9(3): 158-162. [DOI:10.3923/ajps.2010.158.162]
24. Kaur, H., Petla, B.P., Kamble, N.U., Singh, A., Rao, V., SalviGhosh, P. and Majee, M. 2015. Differentially expressed seed aging responsive heat shock protein OsHSP18.2 implicates in seed vigor, longevity and improves germination and seedling establishment under abiotic stress. Frontiers in Plant Science, 6: 713-713. [DOI:10.3389/fpls.2015.00713] [PMID] [PMCID]
25. Kavoosi, M. and Allahgholipour, M. 2017. Effect of rate and split application of nitrogen fertilizer on growth and grain yield of rice (Oryza sativa L.) cvs. Gilaneh and Abjiboji. Iranian Journal of Crop Sciences, 19(2): 165-180. [In Persian with English Summary].
26. Kibinza, S., Bazin, J., Bailly, C., Farrant. J.M., Corbineau, F. and El-Maarouf-Bouteau, H. 2011. Catalase is a key enzyme in seed recovery from ageing during priming. Plant Science, 181: 309-315. [DOI:10.1016/j.plantsci.2011.06.003] [PMID]
27. Kibinza, S., Vinel, D., Côme, D., Bailly, C. and Corbineau, F. 2006. Sunflower seed deterioration as related to moisture content during ageing, energy metabolism and active oxygen species scavenging. Physiologia Plantarum, 128(3): 496-506. [DOI:10.1111/j.1399-3054.2006.00771.x]
28. Kong, Q., Mao, P., Yu, X. and Xia, F. 2014. Physiological changes in oat seeds aged at different moisture contents. Seed Science and Technology, 42(2): 190-201. [DOI:10.15258/sst.2014.42.2.08]
29. Lee, S.S. and Kim, J.H. 2000. Total sugers, -amylase activity, and germination after priming of normal and aged rice seeds. Korean Journal of Crop Science, 45(2): 108-111.
30. Lee, Y.P., Baek, K.H. Lee, H.S., Kwak, S.S., Bang, J.W. and Kwon, S.Y. 2010. Tobacco seeds simultaneously over-expressing Cu/Znsuperoxide dismutase and ascorbate peroxidase display enhanced seed longevity and germination rates under stress conditions. Journal of Experimental Botany, 61(9): 2499-2506. [DOI:10.1093/jxb/erq085] [PMID] [PMCID]
31. Leprince, O., Pellizzaro, A. Berriri, S. and Buitink, J. 2016. Late seed maturation: drying without dying. Journal of Experimental Botany, 68(4): 827-841. [DOI:10.1093/jxb/erw363] [PMID]
32. Li, Y., Wang, Y., Xue, H. Pritchard, H.W. and Wang, X. 2017. Changes in the mitochondrial protein profile due to ROS eruption during ageing of elm (Ulmus pumila L.) seeds. Plant Physiology and Biochemistry, 114: 72-87. [DOI:10.1016/j.plaphy.2017.02.023] [PMID]
33. Maguire, J.D. 1962. Speed of germination-aid in selection and evaluation for seedling emergence and vigor. Crop Science, 2(2): 176-177. [DOI:10.2135/cropsci1962.0011183X000200020033x]
34. McDonald, M.B. 1999. Seed deterioration: physiology, repair and assessment. Seed Science and Technology, 27(1): 177-237.
35. Monajem, S., Zeinali, E., Ghaderi-Far, F., Soltani, E. and Hosseini Chaleshtari, M. 2016. Evaluation of the seed vigour diversity of rice genotypes (Oryza sativa. L.) Journal of Crop Porduction, 8(4): 121-142.
36. Petla, B.P., Kamble, N.U., Kumar, M.,Verma, P., Ghosh, S., Singh, A., Rao, V., Salvi, P., Kaur, H., Saxena, S.C. and. Majee, M. 2016. Rice protein L isoaspartyl methyltransferase isoforms differentially accumulate during seed maturation to restrict deleterious isoAsp and reactive oxygen species accumulation and are implicated in seed vigor and longevity. New Phytologist, 211: 627-645. [DOI:10.1111/nph.13923] [PMID]
37. Rabiei, B. and Mohit, A. 2013. Analysis of Statistical Designs in Agricultural Research (with Emphasis on SAS Software). University of Guilan Press. 427p. [In Persian].
38. Rajjou, L. and Debeaujon, I. 2008. Seed longevity: survival and maintenance of high germination ability of dry seeds. Comptes Rendus Biologies, 331(10): 796-805. [DOI:10.1016/j.crvi.2008.07.021] [PMID]
39. Salvi, P., Saxena, C.S., Petla, B.P., Kamble, N.U., Kaur, H., Verma, P., Rao, V., Ghosh, S. and Majee, M. 2016. Differentially expressed galactinol synthase(s) in chickpea are implicated in seed vigor and longevity by limiting the age induced ROS accumulation. Scientific Reports, 6(1): 1-15. [DOI:10.1038/srep35088] [PMID] [PMCID]
40. Shaaban, M., Ghaderifar, F., Sadeghipour, H. and Yamchi, A. 2017. Ageing mechanisms in chickpea seeds: relationship of sugar hydrolysis and lipid peroxidation with amadori and millard reactions. Iranian Journal of Plant Biology, 9(1): 1-20. [In Persian with English Summary].
41. Sun, Q., Wang, J. and Sun, B. 2007. Advances on seed vigor physiological and genetic mechanisms. Agricultural Sciences in China, 6(9): 1060-1066. [DOI:10.1016/S1671-2927(07)60147-3]
42. Sung, J.M. and Chiu, C.C. 1995. Lipid peroxidation and peroxide-scavenging enzymes of naturally aged soybean seed. Plant Science, 110: 45-52. [DOI:10.1016/0168-9452(95)91223-J]
43. Tilebeni, H.G. and Golpayegani, A. 2011. Effect of seed ageing on physiological and biochemical changes in rice seed (Oryza sativa L.). International Journal of AgriScience, 1(3): 138-143.
44. Velikova, V., Yordanov, I. and. Edreva, A. 2000. Oxidative stress and some antioxidant system in acid-treated bean plants: Protective role of exogenous polyamines. Plant Science, 151(1): 59-66. [DOI:10.1016/S0168-9452(99)00197-1]
45. Walters, C., Ballesteros, D. and Vertucci, V.A. 2010. Structural mechanics of seed deterioration: Standing the test of time. Plant Science, 179: 565-573. [DOI:10.1016/j.plantsci.2010.06.016]
46. Yao, Z., Liu, L., Gao, F., Rampitsch, C., Reinecke, D.M., Ozga, J.A. and Ayele, B.T. 2012. Developmental and seed aging mediated regulation of ntioxidative genes and differential expression of proteins during pre- and post-germinative phases in pea. Journal of Plant Physiology, 169(15): 1477-1488. [DOI:10.1016/j.jplph.2012.06.001] [PMID]
47. Yin, G., Xin. X., Song,C., Chen, X., Zhang, J., Wu, S., Li, R., Liu, X. and Lu, X. 2014. Activity levels and expression of antioxidant enzymes in the ascorbateeglutathione cycle in artificially aged rice seed. Plant Physiology and Biochemistry, 80: 1-9. [DOI:10.1016/j.plaphy.2014.03.006] [PMID]
48. Zamani, A., Nouri, S.A.S. Afshari, R.T., Nezhad, H.I., Akbari, G.A. and. Tavakoli, A. 2010. Lipid peroxidation and antioxidant enzymes activity under natural and accelerated aging in safflower (Carthamus tinctorius L.) seed. Iranian Journal of Field Crop Science, 41(3): 545-554. [In Persian with English Summary].
49. Zhang, Y.X., Xu, H.H., Liu, S.J., Li, N., Wang, W.Q., Møller, I.M. and Song, S.Q. 2016. Proteomic analysis reveals different involvement of embryo and endosperm proteins during aging of Yliangyou 2 hybrid rice seeds. Frontiers in Plant Science, 7: 1-17. [DOI:10.3389/fpls.2016.01394]

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