Volume 8, Issue 2 ((Autumn & Winter) 2022)                   Iranian J. Seed Res. 2022, 8(2): 41-50 | Back to browse issues page

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Department of Agriculture and Plant Breeding, Faculty of Agriculture, Yasouj University, Yasouj, Iran , aminsalehi@yu.ac.ir
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Extended Abstract
Introduction: Schrophularia striata is one of the medicinal plants of the Scrophulariaceae family and contains phenolic compounds. Locals have traditionally used this herbal medicine to treat infections caused by wounds, gastrointestinal diseases, and eye diseases. Due to excessive consumption by indigenous people and climate change, especially increasing temperature, the cold required to eliminate seed dormancy is not presently available and its germination and growth has decreased. Therefore, this plant is at the risk of extinction. Since this plant is propagated in natural habitats through seed and due to deep seed dormancy, evaluation of different seed dormancy methods is necessary for conservation and domestication of this species. In this study, suitable methods to eliminate seed dormancy of this plant using chilling and gibberellic acid treatments were studied on the seeds collected from different habitats of Ilam province.
Materials and Methods: To investigate the effect of using chilling and gibberellic acid on Schrophularia striata dormancy, a split plot factorial experiment based on completely randomized design with four replications was conducted at the seed laboratory of Yasouj University in 2018. The first factor included the duration of moist chilling period (0, 4, 8, 12 and 16 weeks), the second factor included gibberellic acid (0, 300 and 600 mg/L) and the third factor included five habitats (Ilam, Ivan, Mehran, Abadan and Dehloran).
Results: The results showed that the 16-week Chilling and zero gibberellic acid treatments had the highest germination percentage in all habitats, as the percentage of germination in Mehran, Ivan, Ilam, Abadan and Dehloran habitats was 66, 50, 36, 30 and 25%, respectively. Also, the highest germination rate was observed in all habitats at 16 weeks chilling and zero mg/L gibberellic acid concentration. The highest germination uniformity was obtained at 16 and 12 weeks of chilling.
Conclusions: Chilling period duration was effective on germination percentage and rate and with increasing chilling period, germination percentage and rate increased. This indicates that the seeds studied had some degree of physiological dormancy, and the seed of higher altitude habitats required longer chilling periods than those of the lower ones for germination. Also, according to the results of this experiment, chilling periods above 16 weeks should be used to increase germination percentage.

1- Chilling period duration in the presence of gibberellic acid was the most important factor for the elimination of Schrophularia striata seed Dormancy and increased germination.
2- With increasing altitude and in the same chilling period duration, habitats with lower altitude had higher germination percentage and rate
Article number: 3
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Type of Study: Research | Subject: Seed Physiology
Received: 2021/02/26 | Revised: 2024/02/20 | Accepted: 2021/09/18 | ePublished: 2022/05/21

1. Azadbakht, M. 2000. Classification of medicinal plants. Timurzadeh Publications, 420p. [In Persian].
2. Amoaghaei, R.1998. The effect of gibberellin and humid cold on dormancy failure of coma seeds (Ferula ovina Boiss.). Agricultural Science and Technology and Natural Resources, Soil and Water Sciences, 11 (40): 471-481.
3. Baskin, J.M. and Baskin, C.C. 2004. A classification system for seed dormancy. Seed Science Research, 14: 1-16. [DOI:10.1079/SSR2003150]
4. Banaeyan, M. and Najafi, F. 2004. Report on the study of germination characteristics in the seeds of some wild medicinal plants in Iran. Iranian Journal of Seed Research, 2(1): 65-72. [In Persian with English Summary].
5. Cavieres, L.A. and Arroyo M.T.K. 2000. Seed germination response to cold stratification period and thermal regime in Phacelia secunda (Hydrophyllaceae). Plant Ecology, 149(1): 1-8. [DOI:10.1023/A:1009802806674]
6. Copeland, L.O. and McDonald, M.B., 2001. Principles of Seed Science and Technology. Dordrecht, The etherlands: Kluwer Academic Publishers. [DOI:10.1007/978-1-4615-1619-4]
7. Fang, S., Wang, J., Wei, Z. and Zhu, Z. 2006. Methods to break seed dormancy in Cyclocarya paliurus (batal) iljinskaja. Scientia Horticultural, 110(3): 305-309. [DOI:10.1016/j.scienta.2006.06.031]
8. Farhudi, R. and Mekizadeh Tafti, M. 2013. Sleep failure study of mountain beetles affected by gibberellic acid and cold thymus. Iranian Journal of Seed Science and Technology, 3(2): 241-249. [In Persian with English Summary].
9. Finch-Savage, B. 2013. Seeds: Physiology of development, germination and dormancy. In: Bewley, J.D., Bradford, K.J., Hilhorst, H.W.M. and Nonogaki, H. Springer, New York-Heidelberg Dordrecht-London. 392p. Seed Science Research, 23(4): 289-289. [DOI:10.1017/S0960258513000287]
10. Irvani, N., Solouki, M., Omidi, M., Saidi, A. and Zare, A. 2012. Seed germination and dormancy breaking in Drama ammoniacum L., an endangered medicinal plant. Trakya Journal of Sciences, 10(1): 9-15.
11. ISTA, 2008. The international rules for seed testing. International Seed testing Association, 138p.
12. Karavani, B., Afshari, R.T., Hosseini, N.M., Oveisi, M. and Miranshahi, B. 2015. Assessment of seed dormancy in Scrophularia striata. Seed Science and Technology, 44(1): 218-223. [In Persian with English Summary]. [DOI:10.15258/sst.2016.44.1.18]
13. Keshtcar, H.R, Azarniwand, H. and Shahreari, A. 2011. The effect of some treatments on sleep failure and germination of Ferula gummosa and Ferula assa- foetida seeds. Rangeland Scientific Journal, 3(2): 281-29. [In Persian with English Summary].
14. Mohammadpour, M. and Gorbani, H. 2005. Ecological Characteristics and Habitats of Seven Important Medicinal Species of Ilam Province. Conference on Sustainable Development of Medicinal Plants, August 2005, Mashhad. 141-142. ]In Persian [
15. Mozaffarian, and. 2006. Plant Flora of Ilam. Institute of Forestry and Rangelands Research.
16. Nabaei, M., Roshandel, P. and Mohammadkhani, A. 2011. Effective methods for sleep failure and increased germination of Rheum ribes L. Iranian Journal of Medicinal and Aromatics Plant Research, 23(2): 212-222. [In Persian with English Summary].
17. Nemati A, Sharifi H, Gerdakaneh M, Sharifi Z. 2016. The effect of pre-chilling and gibberellic acid on breaking seed dormancy of two medicinal plants species Silybum Mrianum and Citrulus Colocynthis. Iranian Journal of Seed Research, 3(1): 169-177. [In Persian with English Summary].
18. Rezaei, N. 2016. Study of wet cooling and potassium nitrate on dormancy failure of celery seeds (Kelussia odoratissima Mozaff.). Third National Conference on Agriculture and Sustainable Natural Resources, Tehran. [In Persian].
19. Salehi, A., Masumiasl, A. and Moradi, A. 2015. Evaluation of the effective methods of seed dormancy breaking in medicinal plant of Bilhar (Dorema aucheri). Iranian Journal of Seed Research, 2(1): 65-72. [In Persian with English Summary].
20. Sharifi, H., Khajah-Hosseini, M. and Rashed-Mohassel, M.H. 2015. Study of seed dormancy in seven medicinal species from Apiaceae. Iranian Journal of Seed Research, 2(1): 25-36. [In Persian with English Summary].
21. Tajbakhsh, M. 1997. Seed (Cognition-Certification and Control). Ahrar Tabriz Publications, 182P. [In Persian].
22. Tipirdamaz, R. and Gomurgen, N. 2000. The effects of temperature and gibberellic acid on germination of Eranthis hyemalis (L.) Salisb. seeds. Turkish Journal of Botany, 24(2): 143-146.
23. Verma, S.K., Bjpai, G.C. Tewari, S.K. and Singh, J. 2005. Seedling index and yield as influenced by seed size in pigeon pea. Legume Research, 28(2): 143-145.

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