Volume 8, Issue 1 ((Spring and Summer) 2021)                   Iranian J. Seed Res. 2021, 8(1): 1-22 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Mansouri M, Moradi A, Balouchi H, Soltani E. (2021). Effect of Osmotic Potential on Seed Germination and Biochemical Indices of different Christ's thorn (Ziziphus spina–christi) Populations. Iranian J. Seed Res.. 8(1), 1-22. doi:10.52547/yujs.8.1.1
URL: http://yujs.yu.ac.ir/jisr/article-1-436-en.html
Yasouj University , amoradi@yu.ac.ir
Abstract:   (4166 Views)
Extended abstract
Introduction: Seed is the most important sexual reproductive factor in plants that plays an important role in transmitting hereditary characteristics, plant distribution, and establishment in different regions. Seed germination as one of the most important and complex stages in the life cycle of plants is affected by genetic and environmental factors. Drought stress is one of the main barriers of crop plants and trees, production in many parts of the world, especially in arid and semi-arid regions such as Iran. The purpose of this study was to investigate the effect of drought stress on biochemical and seed germination indices of different seed lot of Ziziphus spina–christi as suitable medicinal species for controlling soil erosion in arid regions.
Materials and Methods: This experiment was conducted as a factorial based on a completely randomized design with four replications at the Seed Science and Technology Laboratory of Yasouj University in 2018. Experimental factors included Ziziphus spina–Christi seeds collected from the regions of Minab, Kazeroon, Masjed Soleiman, and Dehdasht and five water potentials including zero, -2, -4, -6, and -8 bar.
Results: In the present study, the effect of osmotic potential, seed lot, and their interaction were significant in each seed lot on germination (germination percentage, germination rate, seedling length, allometric index, longitudinal vigor) and biochemical (hydrogen peroxide, soluble protein content, Proline, catalase, and ascorbate peroxidase) indices of Ziziphus spina–christi. The results showed that except for allometric index response of all studied germination traits to osmotic potential was decreasing, Also with increasing osmotic potential, seed soluble protein was decreased and the amount of proline and activity of catalase and ascorbate peroxidase increased. With increasing osmotic stress, the root length was significantly increased, which increased the allometric coefficient of the seedling.
Conclusion: Germination and biochemical indices of seeds of all seedlots had significant changes with the reduction of osmotic potential and the reduction of osmotic potential was associated with reduced germination and plant establishment capability. These results can help us to recognize the prerequisites of germination and the development of the forests.

1- Germination indices of seeds related to several Ziziphus spinachristi seed lots were investigated under different water potentials.
2- Biochemical aspects related to seed dormancy in several Ziziphus spinachristi seed lots were evaluated.
Article number: 1
Full-Text [PDF 948 kb]   (879 Downloads)    
Type of Study: Research | Subject: Seed Physiology
Received: 2019/09/3 | Accepted: 2020/02/1

1. Abdul-Baki, A.A. and Anderson, J.D. 1973. Vigor determination in soybean seed by multiple criteria. Crop Science, 13(6): 630-633. [DOI:10.2135/cropsci1973.0011183X001300060013x]
2. Aebi, H. 1984. Catalase in vitro. In: Methods in Enzymology. Elsevier, 105: 121-126. [DOI:10.1016/S0076-6879(84)05016-3]
3. Ahmadi, K. and Omidi, H. 2018. The effect of drought stress on physiological traits, peroxidase activity and grain yield of five populations of Lallemantia royleana Benth. Iranian Journal of Medicinal and Aromatic Plants, 34(3): 412-429. [In Persian with English Summary].
4. Akram Ghaderi, R., Soltani, E. and Sadeghipor, H.R. 2014. Biochemical changes in Pumpkin seeds affected by premature aging. Third Congress of Medicinal Plants. Shahed University, Tehran. [In Persian, with English Summary].
5. Al-Hassan, M., Chaura, J., Donat-Torres, M.P., Boscaiu, M. and Vicente, O. 2017. Antioxidant responses under salinity and drought in three closely related wild monocots with different ecological optima. AoB Plants, 9(2): 1-20. [DOI:10.1093/aobpla/plx009] [PMID] [PMCID]
6. Alexieva, V., Sergiev, I., Mapelli, S. and Karanov, E. 2001. The effect of drought and ultraviolet radiation on growth and stress markers in pea and wheat. Plant, Cell and Environment, 24(12): 1337-1344. [DOI:10.1046/j.1365-3040.2001.00778.x]
7. Amiri, M. B., Rzvani-mohgadam, P., Ehyaei, H. M., Fallahi, J. and Aghhavani-shajari, M. 2009. Effect of osmotic and salinity stresses on germination indices and seedling growth of two medicinal plants Cynara scoolymus and Echinacea purpurea. Journal of Environmental Stress in Crop Sciences, 3(2): 165-176. [In Persian, with English Summary].
8. Anjum, S. A., Tanveer, M. and Ashraf, U. 2016. Effect of progressive drought stress on growth, leaf gas exchange, and antioxidant production in two maize cultivars, Environmental Science and Pollution Research, 23(17): 17132-17141. [DOI:10.1007/s11356-016-6894-8] [PMID]
9. Aran, M., Abedi, B., Tehranifar, A. and Parsa, M. 2017. Effects of drought stress on some morphological and physiological properties of three grapevine cultivars (Vitis vinifera L.). Journal of Horticulture Science, 31(2): 315-326. [In Persian with English Summary].
10. Ashraf, M.A., Rasheed, R., Hussain, I., Iqbal, M., Haider, M.Z., Parveen, S. and Sajid, M.A. 2015. Hydrogen peroxide modulates antioxidant system and nutrient relation in maize (Zea mays L.) under water-deficit conditions. Archives of Agronomy and Soil Science, 61(4): 507-523. [DOI:10.1080/03650340.2014.938644]
11. Askari, E., Ehsanzade, P. and Zeinali, H. 2015. Physiological and developmental responses of twelve Foeniculum vulgare Mill genotypes to water potential in the germination stage. Journal of Plant Process and Function, 14(4): 1-16 [In Persian with English Summary].
12. Bailly, C., Benamar, A., Corbineau, F. and Come, D. 2000. Antioxidant systems in sunflower (Helianthus annuus L.) seeds as affected by priming. Seed Science Research, 10: 35-42. [DOI:10.1017/S0960258500000040]
13. Bloch, K.E. Shichman, M. Vorobeychik, D. Vardi, P. 2007. Catalase expression in pancreatic alpha cells of diabetic and non-diabetic mice. Histochemistry and Cell Biology 127: 227-232. [DOI:10.1007/s00418-006-0248-4] [PMID]
14. Bradford, K.J. 2002. Applications of hydrothermal time to quantifying and modeling seed germination and dormancy. Weed Science, 50(2): 248-260. [DOI:10.1614/0043-1745(2002)050[0248:AOHTTQ]2.0.CO;2]
15. Browicz, K. 1977: Ziziphus in K.H. Rechinger Flora Iraniaca. 125: 4-9.
16. Chakrabarty, A., Aditya, M., Dey, N. and Banik, N. 2016. Antioxidant signaling and redox regulation in drought and salinity stressed plants: In Drought Stress Tolerance in Plants, 1: 465-498. [DOI:10.1007/978-3-319-28899-4_20]
17. Date, J., Vandenabeele, S., Vranova, E., Van Montagu, M., Inze, D. and Van Breusegem, F. 2000. Dual action of active oxygen species during plant stress responses. Cellular Molecular of Life Science 57: 779-795. [DOI:10.1007/s000180050041] [PMID]
18. Devi, M.A. and Giridhar, P. 2015. Variations in physiological response, lipid peroxidation, antioxidant enzyme activities, proline and isoflavones content in soybean varieties subjected to drought stress. Proceedings of the National Academy of Sciences, India Section B: Biological Sciences, 85(1): 35-44. [DOI:10.1007/s40011-013-0244-0]
19. Ebadi, M., Azizi, M. and Farzaneh, A. 2011. Effect of drought stress on germination factors of four improved cultivars of German chamomile (Matricaria recutita L.). Journal of Plant Production, 18(2): 119-131. [In Persian with English Summary].
20. Farhadi, H., Azizi, M. and Neemati, H. 2016. Evaluation of germination characteristics of Fenugreek medicinal plants under salinity and drought stress. Journal of Horticultural Science, 31(1): 49-60. [In Persian with English Summary].
21. Finch-Savage, W., Dent, K. and Clark, L. 2004. Soak conditions and temperature following sowing influence the response of maize (Zea mays L.) seeds to on farm priming (pre-sowing seed soak). Field Crops Research, 90(2-3): 361-374. [DOI:10.1016/j.fcr.2004.04.006]
22. Gholipoor, S. and Ebadi, A. 2018. Study of adaptation metabolites and antioxidant enzyme activity of wheat genotypes under moisture stress conditions. Journal of Plant Process and Function, 6: 219-232. [In Persian with English Summary].
23. Ghotbi, A. and Farajzade-tabrizi, E. 2018. Evaluation of seed priming with different nutrient solutions at different water stress levels physiological characteristics and yield of sugar beet genetic sequence cultivar. Journal of Environmental Stresses in Crop Sciences, 11(1): 117-126. [In Persian with English Summary].
24. Goraghani, H.S., Fordoei, A., Sardo, M. and Mahdavi, M. 2017. Effect of salinity and drought stresses on germination stage and growth of black cumin (Bunium persicum Boiss). Iranian Journal of Field Crops Research, 15(1): 1-7. [In Persian with English Summary].
25. Hashemi, M., Azarnivand, H., Assare, M. H., Ashraf-gaefari, A. and Tavili, A. 2014. Effect of drought stress on germination and plant growth indices of three rangeland plant genotypes Agropyron podperae. Pasturage Scientific Journal, 8(3): 212-218. [In Persian with English Summary].
26. Hussain, H.A., Hussain, S., Khaliq, A., Ashraf, U., Anjum, S.A., Men, S. and Wang, L. 2018. Chilling and drought stresses in crop plants: Implications, cross talk, and potential management opportunities. Frontiers in Plant Science, 9: 393. [DOI:10.3389/fpls.2018.00393] [PMID] [PMCID]
27. Irigoyen, J., Einerich, D., and Sánchez Diaz, M. 1992. Water stress induced changes in concentrations of proline and total soluble sugars in nodulated alfalfa (Medicago sativa) plants. Physiologia Plantarum, 84(1): 55-60. https://doi.org/10.1111/j.1399-3054.1992.tb08764.x [DOI:10.1034/j.1399-3054.1992.840109.x]
28. Kafi, M., Nezami, A., Hoseyni, H. and Masoomi, A. 2005. Physiological effects of drought stress by polyethylene glycol on germination of lentil (Lens culinaris Medik.) genotypes. Journal of Iranian Field Crop Research, 3: 69-81. [In Persian with English Summary].
29. Kafi, M., Borzoee, A., Salehi, M., Kamandi, A., Masoumi, A. and Nabati, J. 2012. Physiology of environmental stresses in plants. Ferdowsi University of Mashhad Publication, 502p. [In Persian].
30. Kar, M. and Mishra, D. 1976. Catalase, peroxidase, and polyphenol oxidase activities during rice leaf senescence. Plant Physiology, 57(2): 315-319. [DOI:10.1104/pp.57.2.315] [PMID] [PMCID]
31. Khatami, S.R., Sedghi, M., Garibani, H.M. and Ghahremani, S. 2017. The effect of priming on seed germination characteristics of maize under salt stress. Annals of West University of Timisoara. Series of Biology, 20(1): 65. [In Persian, with English Summary].
32. Kurutas, E.B. 2016. The importance of antioxidants which play the role in cellular response against oxidative/nitrosative stress: Current state.15, 1-22. [DOI:10.1186/s12937-016-0186-5] [PMID] [PMCID]
33. Li, J., Cang, Z., Jiao, F., Bai, X., Zhang, D. and Zhai, R. 2017. Influence of drought stress on photosynthetic characteristics and protective enzymes of potato at seedling stage. Journal of the Saudi Society of Agricultural Sciences, 16(1): 82-88. [DOI:10.1016/j.jssas.2015.03.001]
34. Maghsoodi, M., Razmjo, J. and Gheysari, M. 2016. Effect of drought on biochemical properties of root and shoot of alfalfa cultivars (Medicago sativa L.). Journal of Arid Biome, 6(1):31-43[In Persian with English Summery].
35. Maguire, J.D. 1962. Speed of germination-aid in selection and evaluation for seedling emergence and vigor 1. Crop Science, 2(2): 176-177. [DOI:10.2135/cropsci1962.0011183X000200020033x]
36. Mansouri, M. and Moradi, A. 2018. Effect of temperature and gibberellic acid on breaking seed dormancy and herbaceous indices of four wild populations (Ziziphus spina-christi). The second International Conference on Medicinal Plants, Organic Farming, Natural and medicinal materials. [In Persian with English Summary].
37. Mauad, M., Crusciol, C.A.C., Nascente, A.S., Grassi Filho, H. and Lima, G.P.P. 2016. Effects of silicon and drought stress on biochemical characteristics of leaves of upland rice cultivars. Revista Ciência Agronômica, 47(3): 532-539. [DOI:10.5935/1806-6690.20160064]
38. Michel, B.E. and Kaufmann, M.R. 1973. The osmotic potential of polyethylene glycol 6000. Plant Physiology, 51(5): 914-916. [DOI:10.1104/pp.51.5.914] [PMID] [PMCID]
39. Moradi, P. 2018. Effect of drought stress on Thymus growth and hormonal changes. Journal of Plant Process and Function, 6: 311-323. [In Persian with English Summary].
40. Nadeem, M., Li, J., Yahya, M., Sher, A., Ma, C., Wang, X. and Qiu, L. 2019. Research progress and perspective on drought stress in legumes: A review. International Journal of Molecular Sciences, 20(10): 2541. [DOI:10.3390/ijms20102541] [PMID] [PMCID]
41. Naeemi, T., Fahmide, L. and Fakheri, B. A. 2018. The impact of drought stress on antioxidant enzymes activities, containing of proline and carbohydrate in some genotypes of durum wheat (Triticum turgidum L.) at seedling stage. Journal of Crop Breeding, 10(26): 22-31. [In Persian with English Summary]. [DOI:10.29252/jcb.10.26.22]
42. Nakano, Y. and K. Asada. 1981. Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplast. Plant and Cell Physiology, 22: 867-880.
43. Nonogaki, H. 2006. Seed germination-the biochemical and molecular mechanisms. Breeding Science, 56(2): 93-105. [DOI:10.1270/jsbbs.56.93]
44. Patane, C., Saita, A., Tubeileh, A., Cosentino, S.L. and Cavallaro, V. 2016. Modeling seed germination of unprimed and primed seeds of sweet sorghum under peg induced water stress through the hydrotime analysis. Acta Physiol Plant, 38: 115. [DOI:10.1007/s11738-016-2135-5]
45. Queiroz, R.J. and Cazetta, J.O. 2016. Proline and trehalose in maize seeds germinating under low osmotic potentials. Revista Brasileira de Engenharia Agrícola e Ambiental, 20(1): 22-28. [DOI:10.1590/1807-1929/agriambi.v20n1p22-28]
46. Rahdari, P., Hosseini, S.M. and Tavakoli, S. 2012. The studying effect of drought stress on germination, proline, sugar, lipid, protein and chlorophyll content in purslane (Portulaca oleracea L.) leaves. Journal of Medicinal Plants Research, 6(9): 1539-1547. [DOI:10.5897/JMPR11.698]
47. Reddy, Y.T.N. and M.M. Khan. 2001. Effect of osmopriming on germination, seedling growth and vigour of khirni (Mimusops hexandra) seeds. Seed Research, 29(1): 24-27.
48. Ren, J., Sun, L.N., Zhang, Q.Y. and Song, X.S. 2016. Drought tolerance is correlated with the activity of antioxidant enzymes in Cerasus humilis seedlings. BioMed Research International, 2016: 9851095. [DOI:10.1155/2016/9851095] [PMID] [PMCID]
49. Saburi-rad, S., Kafi, M., Nezami, A. and Bannayan-aval, M. 2011. Study on seed germination behavior of Kochia scoparia L. Schard in response to temperature and water potential. Iranian Journal of Range and Desert Research, 18(4): 578-592. [In Persian with English Summary].
50. Sahitya, U.L., Krishna, M., Deepthi, R., Prasad, G.S. and Kasim, D. 2018. Seed antioxidants interplay with drought stress tolerance indices in chilli (Capsicum annuum L.) seedlings. BioMed Research International, 2018. [DOI:10.1155/2018/1605096] [PMID] [PMCID]
51. Sofo, A., Scopa, A., Nuzzaci, M. and Vitti, A. 2015. Ascorbate peroxidase and catalase activities and their genetic regulation in plants subjected to drought and salinity stresses. International Journal of Molecular Sciences, 16(6): 13561-13578. [DOI:10.3390/ijms160613561] [PMID] [PMCID]
52. Springer, T. 2005. Germination and early seedling growth of chaffy-seeded grasses at negative water potentials. Crop Science, 45(5): 2075-2080. [DOI:10.2135/cropsci2005.0061]
53. Sziderics, A., Rasche, F., Trognitz, F., Sessitsch, A. and Wilhelm, E. 2007. Bacterial endophytes contribute to abiotic stress adaptation in pepper plants (Capsicum annuum L.). Canadian Journal of Microbiology, 53(11): 1195-1202. [DOI:10.1139/W07-082] [PMID]
54. Taghvaei, M., Sadeghi, H. and Bazrafcan, M. 2017. The effect of drought stress on the seed germination of Calotropis procera L. and improvement of drought tolerance. Journal of Plant Research (Iranian Journal of Biology), 30(1), 37-46. [In Persian with English Summary].
55. Tajlil, A.H., Pazoki, A. and Eradatmand Asli, D. 2014. Effects of seed priming by mannitol and zinc sulfate on biochemical parameters and seed germination of chickpea. International Journal of Farming and Allied Sciences, 3: 294-298.
56. Takhti, S. and Shekafandeh, A. 2012. Effect of different seed priming on germination rate and seedling growth of Ziziphus Spina-Christi. Advances in Environmental Biology, 6(1): 159-164.
57. Tani, E., Chronopoulou, E.G., Labrou, N.E., Sarri, E., Goufa, Μ., Vaharidi, X., Tornesaki, A., Psychogiou, M., Bebeli, P.J. and Abraham, Ε.M. 2019. Growth, physiological, biochemical, and transcriptional responses to drought stress in seedlings of Medicago sativa L., Medicago arborea L. and their hybrid (alborea). Agronomy, 9(1): 38. [DOI:10.3390/agronomy9010038]
58. Trautwein, E.A., Reickhoff, D. and Erbershobler, H.F. 1997. The cholesterol- lowering effect of Psyllium a source dietary fiber. Ernhurung Umschau, 44: 214 216.
59. Windauer, L., Altuna, A., and Benech-Arnold, R. 2007. Hydrotime analysis of Lesquerella fendleri seed germination responses to priming treatments. Industrial Crops and Products, 25(1): 70-74. [DOI:10.1016/j.indcrop.2006.07.004]
60. Wu, L.M., Fang, Y., Yang, H.N. and Bai, L.Y. 2019. Effects of drought stress on seed germination and growth physiology of quinclorac-resistant Echinochloa crusgalli. PloS one, 14(4): e0214480. [DOI:10.1371/journal.pone.0214480] [PMID] [PMCID]
61. Yagmur, M. and Kaydan, D. 2008. Alleviation of osmotic stress of water and salt in germination and seedling growth of triticale with seed priming treatments. African Journal of Biotechnology 7: 2156-2162.
62. Zhang, C., Shi, S., Liu, Z., Yang, F. and Yin, G. 2019. Drought tolerance in alfalfa (Medicago sativa L.) varieties is associated with enhanced antioxidative protection and declined lipid peroxidation. Journal of Plant Physiology, 232: 226-240. [DOI:10.1016/j.jplph.2018.10.023] [PMID]

Add your comments about this article : Your username or Email:

Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2023 CC BY-NC 4.0 | Iranian Journal of Seed Research

Designed & Developed by : Yektaweb

This work is licensed under a Creative Commons Attribution 4.0 International License.