Volume 6, Issue 1 ((Spring and Summer) 2019)                   Iranian J. Seed Res. 2019, 6(1): 77-93 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Hojati Fahim N, Sedghi M, Chaeichi M, Seyed sharifi R. The Effect of Seed Inoculation with Organic and Biologic Fertilizers on Germination and Heterotrophic Seedling Indices in Rainfed Wheat (Triticum aestivum) Cultivar. Iranian J. Seed Res.. 2019; 6 (1) :77-93
URL: http://yujs.yu.ac.ir/jisr/article-1-331-en.html
Seed and Plant Certification and Registration Institute , narjes.hojati@yahoo.com
Abstract:   (40 Views)
DOR: 98.1000/2383-1251.1398. 1575.1605

Extended Abstract
Introduction: Iran is located in the arid and semi-arid regions of the world with an average rainfall of 240 mm per year, which requires rethinking of the adoption of methods. One of the alternative ways is to use organic and biological fertilizers. Biological fertilizers are considered as the main and the most important factor in the integrated management of plant foods for sustainable agriculture as they play an important role in product improvement and efficiency. Therefore, this research was carried out with the aim of investigating the effect of seed pre-treatment with some organic and biological fertilizers in rainfed wheat.
Materials and Methods: A factorial experiment with three replications was conducted at the Laboratory of Seed and Plant Certification and Registration of the Center of Agriculture and Natural Resources Research located in Hamedan. Seed inoculation was considered in 5 levels (Seafull, Disper Root Gs., Bio-Health, Trichodermin and control) on 8 different rainfed wheat cultivars (Azar-2, Hashtrood, Baran, Rasad, Owhadi, Sardari, Takab and Homa). First, the seeds were disinfected with sodium hypochlorite, and were then cultured in special containers and were placed in the germinator at 20 ° C. After 4 and 8 days, the number of germinated seeds was counted. Germination seeds were counted in each treatment and germination indices such as germination rate, average daily germination, coefficient germination rate, longitudinal vigor index, weight vigor index, seedling fresh weight, root length/shoot ratio, shoot fresh weight, root fresh weight, seed reserve utilization rate, seed reserve utilization efficiency, fraction utilization seed reserve, seed dry weight were calculated.
Results: With application of different levels of fertilizer, the rate of germination treatment Homa×Bio-Health (155%), average daily germination treatment Hashtrood×Bio-Health (69%), coefficient germination rate treatment Owhadi×Disper Root Gs (60%), longitudinal vigor index treatment Owhadi×Bio-Health (108%), weight vigor index treatment Homa×Bio-Health (64%), root fresh weight treatment Hashtrood×Disper Root Gs (106%), shoot fresh weight treatment Hashtrood×Seafull (23%), seedling fresh weight treatment Homa×Bio-Health (42%), root length/shoot ratio treatment Owhadi×Trichodermin (75%), seed reserve utilization rate treatment Homa×Bio-Health (118%), and fraction utilization seed reserve treatment Homa×Bio-Health (119%) increased, compared with the control. In addition, the application of Bio-Health fertilizer and Hashtrood cultivar had the highest amount in almost all the mentioned attributes
Conclusion: Investigation of the different levels of fertilization showed that in most of the indices related to germination and heterotrophic growth of seedling, pretreatment with Bio-Health biofertilizer had a significant difference with other fertilizer levels.
  1. Evaluation of germination indices for recent cultivars of rainfed wheat, released by rainfed Research Institute of Iran.
  2. Investigating and comparing fertilizers with various multifactorial compounds (fungi and bacteria) and with each other.
  3. Conducting research on commercial compounds and comparison of live biochemical and non-organic matters in a single experiment.
Full-Text [PDF 751 kb]   (10 Downloads)    
Type of Study: Research | Subject: Seed Physiology
Received: 2018/07/12 | Accepted: 2019/01/20

1. Abdul-baki, A.A., and Anderson, J.D., 1973. Vigor determination in soybean seed by multiplication. Crop Science, 13(6): 630-633. [DOI:10.2135/cropsci1973.0011183X001300060013x]
2. Anwar, S., Iqbal, M., Raza, S.H., and Iqbal, N. 2013. Efficacy of seed preconditioning with salicylic and ascorbic acid in increasing vigour of rice (Oryza sativa L.) seedling. Pakistan Journal of Botany, 45(1): 157-162.
3. Azcon, R.J, Barea, M., and Hayman, D.S. 1976. Utilization of rock phosphate in alkaline soil by plantsinoculated with mycorrhizal fungi and phosphate solubilizing bacteria. Soil Biology and Biochemistry, 8(2): 135-138. [DOI:10.1016/0038-0717(76)90078-X]
4. Bajji, M., Kine, J.M., and Stanley, L. 2002. Osmotic and ionic effects of NaCl on germination early seedling growth and ion content of Atriplex halimus. Canadian Journal of Botany, 8(3): 297-304. [DOI:10.1139/b02-008]
5. Bethlenfalavy, G.J., Schreiner, R.P., Mihara, K.L., and McDaniel, H. 1996. Mycorrhizae, biocides, and biocontrol. Mycorrhizal fungi enhance weed control and crop growth in a soybean-cocklebur association treated with the herbicide bentazon. Soil Ecology, 3(3): 205-214. [DOI:10.1016/0929-1393(96)00093-5]
6. Blak, C.A. 2011. Soil Fertility Evaluation and Control. Lewis Publisher, London 415 p.
7. Clausen, R.G., Erturk, N., and Heath, L.S. 2014. Role of superoxide dismutase (SODs) in controlling oxidative stress in plants. Journal of Experimental Botany, 53: 1331-1341. [DOI:10.1093/jxb/53.372.1331] [PMID]
8. Crouch, I., and van Staden, J. 1993. Evidence for the presence of plant growth regulators in commercial seaweed products. Plant Growth Regulation, 13: 21-29. [DOI:10.1007/BF00207588]
9. Diniz, K.A., Oliveira, J.A., Guimarães, R.M., Moreira de Carvalho, M.L., and Machado, j.C. 2006. Incorporation of microrganismos, aminoacidos, micronutrientes e reguladores de crescimento em sementes de alface pela técnica de peliculização. Revista Brasileira de Sementes, 28(3): 37-43. [DOI:10.1590/S0101-31222006000300006]
10. Eyheraguibel, B., Silvestre. J., and Morard, P. 2008. Effects of humic substances derived from organic waste enhancement on the growth and mineral nutrition of maize. Bioresource Technology, 99(10): 4206-4212. [DOI:10.1016/j.biortech.2007.08.082] [PMID]
11. Farooq, M., Basra, S M.A., Rehman, H.U., and Hussain, M. 2008. Seed priming with polyamines improves the germination and early seedling growth in fine rice. Journal of New Seeds, 9(2): 145-155. [DOI:10.1080/15228860802087297]
12. Finnerty, T.L., Zajicek, J.M., and Hussey, M.A. 1992. Use of seed priming to bypass stratification requirements of three Aquilegia species. Horticultural Science, 27(4): 310-313. [DOI:10.21273/HORTSCI.27.4.310]
13. Food and Agriculture Organization. 2017. Statistics: Faostat agriculture. Retrieved June 10, 2017. from http://fao.org/crop/statistics.
14. Gazanchian, A., Khosh Kholgh, N.A., SimaMalboobi, M.A. and Majidi Heravan, E. 2006. Relationships between emergence and soil water content for perennial cool-season grasses native to Iran. Crop Science, 46: 544-553. [DOI:10.2135/cropsci2005.04-0357]
15. Gutierrez-Manero, F.J., Ramos-Solano. B., Probanza. A., Mehouachi. J., Tadeo. F.R., and Talon. M. 2001. The plant-growth promoting rhizobacteria Bacillus pumilus and Bacillus licheniformis produce high amounts of physiologically active gibberellin. Plant Physiology, 111(2): 206-211. [DOI:10.1034/j.1399-3054.2001.1110211.x]
16. Hampton, J.G., and Tekroy, D.M. 1995. Handbook of Vigor Test Methodes (3rd ed.). The International Seed Testing Association (ISTA). Zurich, Swirztland.
17. Harris, D., Tripathi. R.S., and Joshi. A. 2000. On-farm seed priming to improve crop establishment and yield in direct-seeded rice, in IRRI: International Workshop on Dry-seeded Rice Technology', held in Bangkok, 25-28 January 2000. International Rice Research Institute, Manila, Philippines, 164.
18. Hassan, F.M., Yaseen, A.A and Abed, R.K. 2004. Effect of some medicinal plants extracts on the growth of the alga Microcystis aeruginosa Kuetz. Iraqi Journal of Science, 45(1):92-98.
19. Hoogenboom, G., Huck, M.G., and Peterson, C.M. 1987. Root growth rate of soybean as affected by drought stress. Agronomy Journal, 79(4): 607-614. https://doi.org/10.2134/agronj1987.00021962007900040003x [DOI:10.2134/agronj1987.00021962007900040004x]
20. Hossain, I., Yeasmin, R., and Hossain, M.M. 2009. Management of seedling diseaaes of blackgram, mungbean and lentil using BAU- Biofungicide, Biofertilizer and cowdung. Eco-friendly Agriculture Journal, 2(11): 905-910.
21. Ismail, M.A. 2014. Exogenous proline induce changes in SDS-PAGE protein profile for salt tolerance in wheat (Triticum aestivum L.) seedlings. The Research Journal of Pharmaceutical, Biological and Chemical Sciences, 5: 748-752.
22. ISTA. 2006. International Rules for Seed Testing. Edition 2006. International Seed Testing Association, Switzerland.
23. Kumar, G., and Sahoo, D. 2011. Effect of seaweed liquid extract on growth and yield of Triticum aestivum var. Pusa Gold. Journal of Applied Physiology, 23: 251-255. [DOI:10.1007/s10811-011-9660-9]
24. Mackowiak, C.L., Grossl, P.R., and Bugbee, B.G. 2001. Beneficial effects of humic acid on micronutrient availability to wheat. Soil Science, 65(6): 1744-1750. [DOI:10.2136/sssaj2001.1744]
25. Maisuria, K.M., and Patel, S.T. 2009. Seed germinability, root and shoot length and vigour index of soybean as influenced by rhizosphere fungi. Karnataka Journal Agricultural Science, 22(5): 1120-1122.
26. Meena, V.S., Maurya, B.R., and Verma, J.P., 2014. Does a rhizospheric microorganism enhance K+ availability in agricultural soil. Microbiol Research, 169: 337-347. [DOI:10.1016/j.micres.2013.09.003] [PMID]
27. Meena, V.S., Meena, S.K., Verma, J.P., Meena, R.S. and Ghosh, B.N. 2015. The needs of nutrientuse efficiency for sustainable agriculture. Journal of Cleaner Production, 102: 562-563. [DOI:10.1016/j.jclepro.2015.04.044]
28. Mukhtar, I., Hannan, A., Atiq, M., and Nawaz, A. 2012. Impact of Trichoderma species on seed germination in soybean. Pakistan Journal of Phytopathology, 24(2): 159-162.
29. Norrie, J., and Keathley, J. 2006. Benefits of Ascophyllum nodosum marine-plant extract applications to 'Thompson seedless' grape production. Acta Horticulture, 727: 243-245. [DOI:10.17660/ActaHortic.2006.727.27]
30. Nurula, N., Kumar, V., Singh, B., Bhatia, R. and Lashminrayana. K. 2005. Impact of biofertilizers on grain yield in spring wheat under varying fertility conditions and wheat- cotton rotation. Archives of Agronomy and Soil Science, 51(1): 79-89. [DOI:10.1080/03650340400029382]
31. Piccolo, A., Celanoand, G. and Pietramellara, G. 1993. Effects of fractions of coal-derived humic substances on seed germination and growth of seedlings (Lactuca sativa and Lycopersicon esculentum). Biology and Fertility of Soils, 16: 11-15. [DOI:10.1007/BF00336508]
32. Rabie, B., and Bayat, M. 2009. Study parameters of seed germination and seedling growth canola cultivars (Brassica napus L.) by using seed vigor tests. Iranian Journal of Crop Science, 40(1): 93-104. [In Persian with English Summary].
33. Rauthan, B.S., and Schnitzer, M. 1981. Effect of soil fulvic acid on the growth and nutrient content of cucumber (Cucumis sativus) plants. Plant and Soil, 63: 491-495. [DOI:10.1007/BF02370049]
34. Rezaei, M., Sedghi, M., and Seyed Sharifi, R. 2014. Effect of seed priming on reserve mobilization of pot marigold (Calendula officinalis L.) seeds under salinity stress. Research of Crop Ecosystem, 1(2): 67-74. [In Persian with English Summary].
35. Scott, S.J., Jones, R.A., and Williams, W.A. 1984. Review of data analysis method for seed germination. Crop Science, 24: 1192-1199. [DOI:10.2135/cropsci1984.0011183X002400060043x]
36. Sedghi, M., Nemati, A., Amanpour-Balaneji, B. and Gholipouri, A. 2010. Influence of different priming materials on germination and seedling establishment of Milk Thistle (Silybum marianum) under salinity stress. World Applied Sciences Journal, 11(5): 604-609. [In Persian with English Summary].
37. Shahsavari, A., Pirdashti, H., Mottaghian, A., and Tajik-Ghanbari, M.A. 2010. Response of groght propertion and yield of wheat (Triticum aestivum L) on simulations application of manure, Trichoderma (Terichoderma spp) and psudomunas (Psudomunas spp) species. Journal of Agroecology, 2(3): 448-458. [In Persian with English Summary].
38. Sharif, M., Khattak, R.A., and Sarir, M.S. 2002. Effect of different levels of lignitic coal drived humic acid on growth of maize plants. Communication in Soil Science and Plant Analysis, 33(19820): 3567-3580. [In Persian with English Summary]. [DOI:10.1081/CSS-120015906]
39. Sharma, R.Z., Seema, S., Sayyed, B., Trivedi, H., and Thivakaran, A. 2013. Phosphate solubilizing microbes: sustainable approach for managing phosphorus deficiency in agricultural soils. Springer plus, 2: 587-590. [DOI:10.1186/2193-1801-2-587] [PMID] [PMCID]
40. Siadat, S.A., Moosavi, A., and Sharafizadeh, M. 2012. Effect of seed priming on antioxidant activity and germination characteristics of Maize seeds under different aging treatments. Research Journal of Seed Science, 5(2): 51-62. [DOI:10.3923/rjss.2012.51.62]
41. Soltani, A., Galeshi, S., Zenali, E., and. Latifi, N. 2002. Germination seed reserve utilization and growth of chickpea as affected by salinity and seed size. Seed Science and Technology, 30(1): 51-60.
42. Soltani, A., Gholipoor, M., and Zeinali, E. 2006. Seed reserve utilization and seedling growth of wheat as affected by drought and salinity. Environmental and Experimental Botany, 55(1-2): 195-200. [DOI:10.1016/j.envexpbot.2004.10.012]
43. Somani, L.L., Shilpkar, P., and Shilpkar, D. 2011. Biofertilizers: Commercial production technology and quality control. Agrotech Publishing Academy, India.
44. Stevenson, F.J. 1994. Humus Chemistry. John wiley & sons. Inc. New York.
45. Stirk, W., and van Staden, J. 1997. Isolation and identification of cytokinins in a new commercial seaweed product made from Fucus serratus L. Journal of Applied Phycology, 9: 327-330. [DOI:10.1023/A:1007910110045]
46. Vaughan, D., and Linehan, D.J. 1976. The growth of wheat plants in humic acid solutions under axenic conditions. Plant and Soil, 44: 445-449. [DOI:10.1007/BF00015895]
47. Verma, S.K., Bjpai, G.C., Tewari, S.K., and Singh, J. 2005. Seedling index and yield as influenced by seed size in pigeon pea. Legume Research, 28(2): 143-145.
48. Wakjira, K., and Negash, L. 2013. Germination responses of Croton macrostachyus (Euphorbiacea) to various physic-chemical pre-treatment conditions. South African Journal of Botany, 87: 76-83. [DOI:10.1016/j.sajb.2013.03.012]
49. Windham, M.T., Elad, Y., and Baker, K. 1986. A mechanism for increased plant growth inoculated by Tricoderma spp. Phytopathology, 6: 518-521. [DOI:10.1094/Phyto-76-518]
50. Zhang, X. 1997. Influence of plant growth regulators on turfgrass growth, antioxidant status, and drought tolerance. Ph.D. dissertation, Crop and Soil Environmental Sciences, Virginia Polytechnic Institute and State University, USA.

Add your comments about this article : Your username or Email:

Send email to the article author

© 2019 All Rights Reserved | Iranian Journal of Seed Research

Designed & Developed by : Yektaweb

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.