Volume 5, Issue 2 ((Autumn & Winter) 2019)                   Iranian J. Seed Res. 2019, 5(2): 139-149 | Back to browse issues page

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Nastari Nasrabadi H, Moradi M, Modoodi M N. (2019). Effect of Growth Promoting Bacteria and Salicylic Acid on Melon (Cucumis melo) Seed Germination and Seedling Growth under Salt Stress. Iranian J. Seed Res.. 5(2), 139-149. doi:10.29252/yujs.5.2.139
URL: http://yujs.yu.ac.ir/jisr/article-1-326-en.html
Torbat-e-Jam University , ho_nastari@yahoo.com
Abstract:   (11983 Views)

Extended abstract
Introduction: Using of plant growth regulators is one of the methods can improve plant growth against environmental stresses such as salinity. Salicylic acid plays an important role in physiological processes regulation, including germination. Today, using of growth promoting bacteria has been increased and it causes to raise the seed vigor, uniformity, germination percentage and better seedling establishment. Growth promoting bacteria can be effect on increasing plant resistance to adverse environmental conditions by interposition in plant hormones production such as auxin, GA, cytokinins, and as well as the stabilization of nitrogen or phosphorus availability and other nutrients
Materials and Methods: This experiment was conducted as factorial in a completely randomized design with three replications. Salicylic acid factor (SA) was selected at two levels (0 and 1 mM). The bacterial treatments included Azotobacter (AZ), Azospirilum (AZP), complex of Azotobacter and Azospirillum (AZ + AZP), and without inoculation (C) and salinity treatment (S) was at five levels (0, 50, 100, 150 and 200 mM).
Results: Results showed that all treatments had no significant effect on germination percentage. Radicle and plumule length, seed vigor index and seedling fresh weight was significantly increased at 50 mM NaCl. Generally speaking, the elongation of plant organs when treated with low concentrations of salts may induce osmotic adjustment activity in the plants which may improve growth. Germination rate, Radicle and plumule length and seed vigor index were significantly increased by salicylic acid treatment. AZ and AZ+AZP increased germination parameters significantly than control. Generally germination factors were better improved by combination salicylic acid with AZ than AZP and AZ+AZP. These results could indicate the synergistic relationship between growth promoting bacteria and salicylic acid.
 Conclusion: According to the results pre-treatment of melon seeds by 1 mM salicylic acid and Azotobacter can be proposed to improve seed germination and seedling establishment under salinity stress.

  1. Effect of salinity on seed germination characteristics of melon.
  2. Effect of biofertilizer and salicylic acid on germination and seedling growth of melon under salt stress.
Full-Text [PDF 489 kb]   (1446 Downloads)    
Type of Study: Research | Subject: Seed Physiology
Received: 2018/03/19 | Accepted: 2018/09/15

1. Abdul Qados, A.M.S. 2011. Effect of salt stress on plant growth and metabolism of bean plant Vicia faba L. Journal of the Saudi Society of Agricultural Sciences, 10: 7-15. [DOI:10.1016/j.jssas.2010.06.002]
2. Ansari, A., Shahgoli, H., Makarian, H., and Fallah Nosratabad, A.R. 2015. Evaluation of the effects of plant growth promoting rhizobacteria and salinity on germination and growth of corn plants (Zea mays L.). Journal of Soil Management and Sustainable Production, 4(4): 235-253. [In Persian with English Summary].
3. Azad, H., Fazeli-nasab, B., and Sobhanizade, A. 2017. A study into the effect of jasmonic and humic acids on some germination characteristics of rosselle (Hibiscus sabdariffa) seed under salinity stress. Iranian Journal of Seed Research, 4(1):1-18. [In Persian with English Summary]. [DOI:10.29252/yujs.4.1.1]
4. Bashan, Y., Holguin, G., and De-Bashan, L. 2004. Azosprillum-plant relationship: physiological, molecular, agricultural, and environmental advances. Canadian Journal of Microbiology, 50(8): 521-577. [DOI:10.1139/w04-035] [PMID]
5. Dantas, B.F., Ribeiro, L., and Aragao, C.A. 2005. Physiological response of cowpea seeds to salinity stress. Revista Brasileria de Sementes, 27(1): 144-148. [DOI:10.1590/S0101-31222005000100018]
6. El-Tayeb, M.A. 2005. Response of barley grains to the interactive effect of salinity and salicylic acid. Plant Growth Regulation, 45: 215-225. [DOI:10.1007/s10725-005-4928-1]
7. Hafeez, F.Y., Safdar, M.E., Chaudry, A.U., and Malik, K.A. 2004. Rhizobial inoculation improves seedling emergence, nutrient uptake and growth of cotton. Australian Journal of Experimental Agriculture, 44: 617-622. [DOI:10.1071/EA03074]
8. Katergi, N., Van Hoorn, J.W., Hamdy, A., Karam, F., and Mastrortilli, M. 1994. Effect of salinity onemergence and on water stress early seedling growth of sunflower and maize. Agricultural Water Management, 26: 81-91. [DOI:10.1016/0378-3774(94)90026-4]
9. Khodary, S.E.A. 2004. Effect of salicylic acid on the growth, photosynthesis and carbohydrate metabolism in salt-stressed maize plant. International Journal of Agriculture and Biology, 6: 5-8.
10. Kokelis-Burelle, N., Kloepper, J.W., and Reddy, M.S. 2006. Plant growth promoting rhizobacteria as transplant amendments and their effects on indigenous rhizosphere microorganisms. Applied Soil Ecology, 31(1-2): 91-100. [DOI:10.1016/j.apsoil.2005.03.007]
11. Krishnamurthy, L., Ito, O., Johansen, C., and Saxsena, N.P. 1998. Length to weight ratio of chickpea roots under progressively reducing soil moisture conditions in a vertical. Field Crops Research, 58: 177-185. [DOI:10.1016/S0378-4290(98)00093-8]
12. Kumar, V., Behl, R.K. and Narula, N. 2001. Establishment of phosphate-solubilizing strains of Azetobacter chroococcum in rhizosphere and their effect on wheat under house conditions. Microbiological Research, 156: 87-93 [DOI:10.1078/0944-5013-00081] [PMID]
13. Lakshminarayana, K. 1993. Influence of azotobacter on nitrogen nutrition of plant and crop productivity. Proc. Indian National Science Academy, 59: 303-308.
14. Maguire, J.D. 1962. Speed of germination-aid in selection and evaluation for seedling emergence and vigor. Crop Science, 2: 176-177. [DOI:10.2135/cropsci1962.0011183X000200020033x]
15. Mayer, A.M., and Polijakoff-Mayber, A. 1989. Effect of salinity on emergence and on water stress early seedling growth of sunflower and maize. Agricultural Water Management, 26: 81-91.
16. Motamednejad, M., Eslami, S.V., Sayari, M.H., and Mahmodi, S. 2016. Effect of enrichment with bio fertilizers and three micronutrients of iron, zinc and manganese on germination characteristics of ajowan plant (Carum copticum L.). Journal of Horticultural Science, 29(4): 564-571. [In Persian with English Summary]
17. Munns, R. 2002. Comparative physiology of salt and water stress. Plant, Cell and Environment, 25: 239-250. [DOI:10.1046/j.0016-8025.2001.00808.x] [PMID]
18. Ng, L.C., Sariah, M., Sariam, O., Radziah, O., and Zainal Abidin, M.A. 2012. Rice seed bacterization for promoting germination and seedling growth under aerobic cultivation system. Australian Journal of Crop Science, 6(1): 170-175.
19. Pal, S.S. 1998. Interaction of an acid tolerant strain of phosphate solubilizing bacteria with a few acid tolerant crops. Plant and Soil, 198: 169-177. [DOI:10.1023/A:1004318814385]
20. Patanea, C., Cavallaroa, V., and Cosentinob, S. 2009. Germination and radicle growth in unprimed and primed seeds of sweet sorghum as affected by reduced water potential in NaCl at different temperatures. Industrial Crops and Products, 30: 1-8. [DOI:10.1016/j.indcrop.2008.12.005]
21. Piao, Z., Cui, Z., Yin, B., Hu, J., Zhou, C., Xie, G., Su, B., and Yin, S. 2005. Changes in acetylene reduction activities and effects of inoculated rhizosphere nitrogen-fixing bacteria on rice. Biology and Fertility of Soils, 41(5): 371-378. [DOI:10.1007/s00374-005-0860-9]
22. Puppala, N., Poindexter, J.L., and Bhardwaj, H.L. 1999. Evaluation of salinity tolerance of canola germination. Perspectives on new crops and new uses. ASHS Press, Alexardria, 251-253.
23. Raskin, I. 1992. Role of salicylic acid in plants. Annual Review of Plant Physiology, 43: 439-463. [DOI:10.1146/annurev.pp.43.060192.002255]
24. Remus, R., Ruppel, S., Jacob, H.J., Hecht-Buchholzch, C., and Merbach, W. 2000. Colonization behavior of two enterobacterial strains on cereals. Biology and Fertility of Soils, 30(5-6): 550-557. [DOI:10.1007/s003740050035]
25. Saatovich, S.Z. 2006. Azospirilli of uzbekistan soils and their influence on growth and development of wheat plants. Plant and Soil, 283(1-2): 137-145. [DOI:10.1007/s11104-005-5690-x]
26. Serraj, R., and Sinclair, T.R. 2002. Osmolyte accumulation: can it really help increase crop yield under drought conditions. Plant, Cell and Environment, 25(2): 333-341. [DOI:10.1046/j.1365-3040.2002.00754.x]
27. Shahin, F., Cakmakci, R., and Kantar, F. 2004. Sugar beet and barley yields in relation to inoculation with N2-fixingand phosphate solubilizing bacteria. Plant and Soil, 265(1-2): 123-129. [DOI:10.1007/s11104-005-0334-8]
28. Sharikova, F., Sakhabutdinova, A., Bezrukova, M., Fatkhutdinova, R., and Fatkhutdinova, D. 2003. Changes in the hormonal status of wheat seedling induced by salicylic acid and salinity. Plant Science, 164(3): 317-322. [DOI:10.1016/S0168-9452(02)00415-6]
29. Singh, B., and Usha, K. 2003. Salicylic acid induced physiological and biochemical changes in wheat seedlings under water stress. Plant Growth Regulation, 39(2): 137-141. https://doi.org/10.1023/A:1022556103536 [DOI:10.1007/s10725-007-9211-1]
30. Sivritepe, N., Sivritepe, H.O., and Eris, A. 2003. The effects of NaCl priming on salt tolerance in melon seedling grown under saline conditions. Sciatica Horticulturae, 97: 229-237. [DOI:10.1016/S0304-4238(02)00198-X]
31. Sundara, B., Natarajan, V. and Hari, K. 2002. Influence of phosphorus solubilizing bacteria on the changes in soil available phosphorus and sugarcane and sugar yield. Field Crops Research, 77: 43-49. [DOI:10.1016/S0378-4290(02)00048-5]
32. Taheri, S.,Barzegar, T., and Zoeam zadeh, A. 2017. Effect of salicylic acid pre-treatment on cucumber and watermelon seeds germination under salinity stress. Iranian Journal of Seed Science and Research, 3(4): 15-27. [In Persian with English Summary].
33. Wang, L., Chen, S., Kong, W., Li, S., and Archbold, D.D. 2006. Salicylic acid pretreatment alleviates chilling injury and affects the antioxidant system and heat shock proteins of peaches during cold storage. Postharvest Biology and Technology, 41(3): 244-251. [DOI:10.1016/j.postharvbio.2006.04.010]
34. Younesi, O., Poustini, K., Chaichi, M.R., and Pourbabaie, A.A. 2012. Effect of growth promoting rhizobacteria on germination and early growth of two alfalfa cultivars under salinity stress condition. Journal of Crops Improvement, 14(2): 83-97. [In Persian with English Summary].
35. Zaidi, A., and Mohammad, S. 2006. Co-inoculation effects of phosphate solubilizing micro-organisms and Glomus fasciculatum on green gram-bradyrhizobium symbiosis. Turkish Journal of Agriculture and Forestry, 30: 223-230.
36. Zhu, J.K. 2002. Salt and drought stress signal transduction in plants. Annual Review of Plant Biology, 153: 247-273. [DOI:10.1146/annurev.arplant.53.091401.143329] [PMID] [PMCID]

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