Volume 6, Issue 1 ((Spring and Summer) 2019)
Iranian J. Seed Res. 2019, 6(1): 33-49 |
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Shahrekord University , aabasi59@yahoo.com
Abstract: (9371 Views)
Extended Abstract
Introduction: In addition to dormancy, seeds of Allium hirtifolium have a weak emergence in the field. Among methods for improving the efficiency and emergence of seeds, nutritional priming can be considered for its performance on weak seeds. The presence of micronutrients is one of the factors that may affect the efficiency of the seeds. Therefore, the aim of this study was to investigate the effect of priming with nutrients on optimization of dormancy status, germination, and enhancement of shallot seeds for its conservational, restoration and domestication programs.
Materials and Methods: In order to study effects of nutrients on germination and emergence of Allium hirtifolium, a CRD factorial experiment was conducted with four replications at Seed Science and Technology Lab of Shahrekord University in 2015. Two dormancy breaking treatments (sulfuric acid and sulfuric acid + gibberellic acid) as the first factor and nine nutrition treatments including four levels of ZnSO4 (5, 10, 50 and 100 mM) and four levels of FeSO4 (0.5, 1, 1.5 and 2%) versus control were compared on shallot seeds.
Results: The results showed that dormancy breaking treatments, nutrient pretreatment of seeds and their interaction had significant effects on germination percentage, germination rate, time to reach l0% and 50% germination, germination uniformity, seedling length and vigor index I at 1% probability level. Sulfuric acid and FeSO41% increased germination versus control. Application of gibberellic acid affected the behavior of iron but did not indicate significant effects for zinc. The concentration of 5 mM ZnSO4 increased the rate of germination, compared with the control but decreased with higher concentrations. The gibberellic acid did not show any sharp effects on germination rate. Time to reach 50% germination was also affected by FeSO4 0.5% and 1% and lower levels of zinc. Application of gibberellic acid did not show any significant impact on the germination time reduction, compared with control and increased T50 in higher concentrations. Although germination traits were rarely affected by gibberellic acid, seedling length and vigor index were positively influenced with GA, and the highest seedling length was achieved at 0.5 and 1% of iron and gibberellic acid.
Conclusion: Seed priming with nutrients can improve germination and plant vigur indices. Different concentrations of iron and zinc showed different impacts on the seeds, which showed interaction with dormancy breaking methods. Although application of gibberellic acid did not have an effective role in increasing germination rate and reducing the time to reach 10% and 50% of germination, it enhanced seedling length and vigor index I, especially for iron.
Highlights:
- Addition of iron and zinc sulfate to shallot seeds whose dormancy was broken with sulfuric acid caused higher germination rate of 25.54%, compared with the control.
- Gibberellin compensated for zinc effect in germination and was able to replace it, but had a slight synergic effect with iron sulfate.
- Although gibberellin application did not affect germination traits, the seedling length and vigor index showed a positive response to it.
Article number: 3
Type of Study: Applicable |
Subject:
General
Received: 2018/09/12 | Revised: 2021/03/13 | Accepted: 2019/02/25 | ePublished: 2019/09/29
Received: 2018/09/12 | Revised: 2021/03/13 | Accepted: 2019/02/25 | ePublished: 2019/09/29
References
1. Abdulrahmani, B., Ghassemi-Golezani, K., Valizadeh, M., and Asl, V.F. 2007. Seed priming and seedling establishment of barley (Hordeum vulgare L.). Journal of Food Agriculture and Environment, 5(3/4): 179-184.
2. Abutalebian, M.A., and Mohagheghi, A. 2016. Effect of different priming treatments on yield and yield components of lentil in season last under drought stress. Journal of Production and Processing of Agricultural and Horticultural Products, 15: 129-140. [In Persian with English Summary]. [DOI:10.18869/acadpub.jcpp.5.15.129]
3. Adhikari, T., Kundu, S., and Rao, A.S. 2015. Zinc delivery to plants through seed coating with nano-zinc oxide particles. Journal of Plant Nutrition, 39(1): 136-146. [DOI:10.1080/01904167.2015.1087562]
4. Aghababanejad, Z. Abbasi Surki, A., and Tahmasebi, P. 2018. Studying interaction of Moist-Chilling and gibberellic acid on germination of Fritillaria imperialis. Iranian journal of seed science and technology 6:257-266. [In Persian with English Summary].
5. Akram Ghaderi, F., Kamkar, B., and Soltani, A. (translators). 2015. Seed Science and Technology. Mashhad University Jahad Publishers. Edition (2). 512 p. [In Persian].
6. Alivand, R., Tavakkol Afshari, R., and Sharifzade, F. 2014. Study of seed germination process of rapeseed (Brassica napus) and Prediction seed disappearance under different storage conditions. Iranian Journal of Crop Sciences, 1: 69-83. [In Persian with English Summary].
7. Allahmoradi, M., Ghanbarian, G. A., and Ghasemi, F. 2013. Investigation of the characteristics of the Allium hirtifolium (Boiss) in Fars. Journal of Rangeland, 4: 282-291. [In Persian with English Summary].
8. Ameri, A., Fatemi, H., Aroiee, H., and da Silva, J.A.T. 2011. What's the Effect of Saline Priming on Germination Factors of Capsicum annuum var. 'California Wonder'Seeds? Seed Science and Biotechnology, 5(1): 47-49.
9. Badiri, A., and Mirshekari, B. 2014. Germination and yield of plantain affected by priming with some micronutrients. International Journal of Advanced Life Sciences, 7: 565-573.
10. Badiri, A., Mirshekari, B., Hadavi, E., and Hamidi, A. 2014. Effect of Seeds Priming with Micronutrients on Growth Seed Yield and Mucilage of Plantain. International Journal of Plant, Animal and Environmental Sciences, 4: 335-342.
11. Bicalho, E.M., Pintó-Marijuan, M., Morales, M., Müller, M,. Munné‐Bosch, S., and Garcia, Q.S. 2015. Control of macaw palm seed germination by the gibberellin/abscisic acid balance. Plant Biology, 17(5): 990-996. [DOI:10.1111/plb.12332] [PMID]
12. Dashti, F., Ghahremani-Majd, H., and Esna-Ashari, M. 2012. Overcoming seed dormancy of mooseer (Allium hirtifolium) through cold stratification, gibberellic acid, and acid scarification. Journal of Forestry Research, 23(4): 707-710. [DOI:10.1007/s11676-012-0314-9]
13. Ebrahimi, R., Zamani, Z., and Kashi, A. 2009. Genetic diversity evaluation of wild Persian shallot (Allium hirtifolium Boiss.) using morphological and RAPD markers. Scientia Horticulturae, 119(4): 345-351. [DOI:10.1016/j.scienta.2008.08.032]
14. Ebrahimi, R., Hassandokht, M., Zamani, Z., Kashi, A., Roldan-Ruiz, I., and Van Bockstaele, E. 2014. Seed morphogenesis and effect of pretreatments on seed germination of Persian shallot (Allium hirtifolium Boiss.), an endangered medicinal plant. Horticulture, Environment, and Biotechnology, 55(1): 19-26. [DOI:10.1007/s13580-014-0032-7]
15. Ellis, R.H., and Roberts, E.H. 1981. The quantification of ageing and survival in orthodox seeds. Seed Science and Technology, 9: 377-409.
16. Etemadi, N., Haghighi, M., and Zamani N. 2011. Optimizing seed germination threatened endemic species of the Persian shallot (Allium hirtifolium boiss.). African Journal of Agricultural Research, 25(6): 5650-5655. [DOI:10.5897/AJAR11.1156]
17. Farooq, M., Wahid, A., and Siddique, K.H. 2012. Micronutrient application through seed treatments: a review. Journal of Soil Science and Plant Nutrition, 12(1): 125-142. [DOI:10.4067/S0718-95162012000100011]
18. Harris, D., Rashid, A., Miraj, G., Arif, M., and Yunas, M. 2008. On-farm seed priming with zinc in chickpea and wheat in Pakistan. Plant and Soil, 306(1-2): 3-10. [DOI:10.1007/s11104-007-9465-4]
19. International Seed Testing Association. 2011. International rules for seed testing. Seed Science and Technology, 24: 155-202.
20. Johnson, S.E., Lauren, J.G., Welch, R.M., and Duxbury, J.M. 2005. A comparison of the effects of micronutrient seed priming and soil fertilization on the mineral nutrition of chickpea (Cicer arietinum), lentil (Lens culinaris), rice (Oryza sativa) and wheat (Triticum aestivum) in Nepal. Experimental Agriculture, 41(4): 427-448. [DOI:10.1017/S0014479705002851]
21. Joosen, R.V.L., Kodde, J., Willems, L.A.J., Ligterink, W., van der Plas, L.H., and Hilhorst, H.W. 2010. Germinator: a software package for high‐throughput scoring and curve fitting of Arabidopsis seed germination. The Plant Journal, 62(1): 148-159. [DOI:10.1111/j.1365-313X.2009.04116.x] [PMID]
22. Kheirkhah, M., and Dadkhah, A. 2010. Study of Allium altissimum Regel. phenology and consider how to domesticating it. Horticulture Researches (Pajouhesh and Sazandegi), 82: 19-24. [In Persian with English Summary].
23. Latef, A.A.H.A., Alhmad, M.F.A., and Abdelfattah, K.E. 2017. The possible roles of priming with ZnO nanoparticles in mitigation of salinity stress in lupine (Lupinus termis) plants. Journal of Plant Growth Regulation, 36(1): 60-70. [DOI:10.1007/s00344-016-9618-x]
24. Lebedev, S.V., Korotkova, A.M., and Osipova, E.A. 2014. Influence of Fe nanoparticles. Magnetite Fe3O4 nanoparticles and iron (II) sulfate (FeSO4) solutions on the content of photosynthetic pigments in Triticum vulgare. Russian Journal of Plant Physiology, 61: 564-569. [DOI:10.1134/S1021443714040128]
25. Makkizade Tafti, M., and Farhudi, B. 2014. Effect of break dormancy treatments on germination and seedling growth of (Kelussia odoratissima Mozaff.). Journal of Plant and Ecosystem, 37: 53-61. [In Persian with English Summary].
26. Medeiros, M.J., Oliveira, M.T., Willadino, L., and Santos, M.G. 2015. Overcoming seed dormancy using gibberellic acid and the performance of young Syagrus coronata plants under severe drought stress and recovery. Plant Physiology and Biochemistry, 97: 278-286. [DOI:10.1016/j.plaphy.2015.10.008] [PMID]
27. Mirshekari, B. 2012. Seed priming with iron and boron enhances germination and yield of dill (Anethum graveolens). Turkish Journal of Agriculture and Forestry, 36(1): 27-33.
28. Mirshekari,. B. 2015. Effects of seed priming with microelements of Fe and B on some germination parameters and yield of marigold (Calendula officinalis L.). Iranian Journal of Medicinal and Aromatic Plants, 30(6): 879-888.
29. Mozaffarian, V. 2012. Identification of Medicinal and Aromatic Plants of Iran. Contemporary Culture of Tehran Press. 1444 p. [In Persian].
30. Muhammad, I., Kolla, M., Volker, R., and Günter, N. 2015. Impact of nutrient seed priming on germination, seedling development, nutritional status and grain yield of maize. Journal of Plant Nutrition, 38(12): 1803-1821. [DOI:10.1080/01904167.2014.990094]
31. Nautiyal, N., and Shukla, K. 2013. Evaluation of seed priming zinc treatments in chickpea for seedling establishment under zinc-deficient conditions. Journal of Plant Nutrition, 36(2): 251-258. [DOI:10.1080/01904167.2012.739245]
32. Payal, K., Maikhuri, R.K., Rao, K.S., and Kandari, L.S. 2014. Effect of gibberellic acid-and water-based pre-soaking treatments under different temperatures and photoperiods on the seed germination of Allium stracheyi Baker: An endangered alpine species of Central Himalaya, India. Plant Biosystems-An International Journal Dealing with all Aspects of Plant Biology, 148(6): 1075-1084. [DOI:10.1080/11263504.2013.823131]
33. Prasad, T.N.V.K.V., Sudhakar, P., Sreenivasulu, Y., Latha, P., Munaswamy, V., Reddy, K.R., Sreeprasad, T.S., Sajanlal, P.R. and Pradeep, T. 2012. Effect of nanoscale zinc oxide particles on the germination, growth and yield of peanut. Journal of Plant Nutrition, 35(6): 905-927. [DOI:10.1080/01904167.2012.663443]
34. Sharifi, R., Mohammadi, K., and Rokhzadi, A. 2016. Effect of seed priming and foliar application with micronutrients on quality of forage corn (Zea mays). Environmental and Experimental Biology, 14: 151-156. [DOI:10.22364/eeb.14.21]
35. Shinde, P., and Vasudevan, S.D.S. 2017. Influence of seed polymer coating with micronutrients and foliar spray on seed yield of chickpea (Cicer arietinum L.). Legume Research, 40(4): 704-709. [DOI:10.18805/lr.v0iOF.10760]
36. Tavakkol Afshari, R., Abbasi surki, A., and Ghasemi, A. 2008. Seed Technology and its Biological Basis. Tehran University Publishers. First Edition. 515 p. [In Persian].
37. Torabian, S., Zahedi, M., and Khoshgoftar, A.H. 2017. Effects of foliar spray of nano-particles of FeSO4 on the growth and ion content of sunflower under saline condition. Journal of Plant Nutrition, 40(5): 615-623. [DOI:10.1080/01904167.2016.1240187]
38. Vojodi, M.L., Hassanpouraghdam, M.B., Ebrahimzadeh, A., and Valizadeh, K.R. 2016. Effects of ZnSO4 foliar application on vegetative growth and phenolic and essential oil content of geranium (Pelargonium odoratissimum L.). Journal of Ornamental Plants, 6: 193-199.
39. Wani, R.A., Malik, T.H., Malik, A.R., Baba, J.A., and Dar, N.A. 2014. Studies on apple seed germination and survival of seedlings as affected by gibberellic acid under cold arid conditions. International Journal of Scientific and Technology Research, 3(3): 2010-2016.
40. Yamauchi, Y., Ogawa, M., Kuwahara, A., Hanada, A., Kamiya, Y., and Yamaguchi, S. 2004. Activation of gibberellin biosynthesis and response pathways by low temperature during imbibition of Arabidopsis thaliana seeds. The Plant Cell, 16(2): 367-378. [DOI:10.1105/tpc.018143] [PMID] [PMCID]
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