Volume 5, Issue 2 ((Autumn & Winter) 2019)                   Iranian J. Seed Res. 2019, 5(2): 43-58 | Back to browse issues page

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Nouriyani H. Effect of Seed Priming on Germination Characteristics, Biochemical Changes and Early Seedling Growth of Sesame (Sesamum indicum). Iranian J. Seed Res.. 2019; 5 (2) :43-58
URL: http://yujs.yu.ac.ir/jisr/article-1-320-en.html
Payame Noor University , h_noryani@yahoo.com
Abstract:   (3048 Views)
DOR: 98.1000/2383-1251.1397.5.

Extended abstract
Introduction: Today, various technologies have been developed to improve seed quality with the aim of increasing the percentage, speed and uniformity of germination and improved seedling establishment under different environmental conditions. One of these technologies is seed pre-treatment or seed priming. In this regard, some studies confirmed that pre-treatment of seeds with hormones and plant growth regulators improve germination behavior and its related indices, including average germination time, seed vigor, radicle length, plumule length, germination rate and seedling establishment in primed seeds of sesame. Cognizant of the sensitivity of the germination process as the first plant developmental stage and the importance of improving germination indices and sesame seedling establishment, the aim of this study was to evaluate the seed priming efficiency, using salicylic acid, methyl jasmonate and humic acid in the germination performance, biochemical changes and early seedling growth of two sesame cultivars including Yellow white and local cultivar of Dezful.
Materials and Methods: This experiment was conducted as a factorial based on a completely randomized design with four replications in the seed technology Laboratory of Safi Abad Dezful Agricultural Research Center in the summer of 2015. The first factor was seed pre-treatment with distilled water (control), salicylic acid 0.1mM, methyl jasmonate 1µM, and humic acid 1.5% and the second factor was two varieties of sesame including Yellow white and the local cultivar of Dezful. Germination percentage, germination rate, seed vigor index, radicle length, plumule length, allometric coefficient, proline content, soluble proteins and catalase enzyme activity were determined to compare the treatments.
Results: The results of the experiment showed that seeds priming had a significant effect on germination percentage, germination rate, seed vigor index, radicle length, plumule length, allometric coefficient and seedling biochemical changes, and improved them. The effect of cultivar on all the traits studied, except mean daily germination and plumule length, was not significant. In this research, the Dezful cultivar had an average of 13.52 seeds per day with higher germination rates, compared with the other cultivar (Yellow white). In addition, the interaction of priming × cultivar was significant only in seed vigor index and allometric coefficient, where the comparison of the mean values indicated that the highest seed vigor was obtained by using humic acid 1.5% in the Dezful cultivar (10.09), while the highest allometric coefficient in seed pre-treatment with methyl jasmonate was found in the Yellow white cultivar (1.57).
Conclusions: In this study, the most effective seed priming treatments for improving germination, biochemical changes and seedling growth of sesame was humic acid 1.5%, recorded for the local cultivar of Dezful. Seed priming with humic acid 1.5% was significantly better than the control and Dezful cultivar’s germination performance was better than that of Yellow white cultivar; therefore it can be said that pre-treatment of seed with humic acid with significant effects on germination characteristics of sesame can be more effective in establishment of seedlings. It is advisable to use this organic acid for better root system development and sesame seedlings establishment, which is a major problem at the beginning of the growing season.
  1. Humic acid was the most effective seed priming treatment on germination characteristics, biochemical changes and seedlings growth of sesame.
  2. The effect of seed priming treatments on the germination characteristics of the Dezful cultivar was more pronounced than that of Yellow white.
Full-Text [PDF 550 kb]   (368 Downloads)    
Type of Study: Applicable | Subject: Seed Physiology
Received: 2018/01/2 | Accepted: 2018/07/6

1. Aboutalebian, M.A., Nazari, Sh., and Golzardi, F. 2017. The effect of seed priming on emergence and root morphological characteristics of canola at different sowing dates. Iranian Journal of Field Crop Science, 47(4): 645-659. [In Persian with English Summary].
2. Alizadeh, M., Balouchi, H.R., and Yadavi, A.R. 2016. Effect of seed priming and irrigation water quality on yield and oil yield components and oil of two sesame genotypes (Sesamum indicum L.). The Plant Production, 39(2): 115-125. [In Persian with English Summary].
3. Asgharipour, M.A., and Rafiei, M. 2011. The effect of different concentrations of humic acid on seed germination behavior and vigor of barley. Australian Journal of Basic and Applied Sciences, 5(12): 610-613.
4. Babaei Qaqlstany, A., Asadi Gakiyeh, M., Fallahi, N., and Hatami Ghare Ghoyni, N. 2016. Osmopriming effect on germination of wheat under allelopathic conditions of foxtail extract. Seed Research, 6(18): 11-19. [In Persian with English Summary].
5. Bates, L.S., Waldren, R.P., and Teare, I.D. 1973. Rapid determination of free proline for water stress studies. Plant Soil, 39: 205-208. [DOI:10.1007/BF00018060]
6. Bekherad, H., Mahdavi, B., and Rahimi, A. 2015. Effect of seed priming on germination, morphological and physiological characteristics of sesame (Sesamum indicum L.) under alkalinity stress. Journal of Plant Production Research, 22(2): 25-46. [In Persian with English Summary].
7. Bradford, M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Annual Biochemistry, 72: 248-254. [DOI:10.1016/0003-2697(76)90527-3]
8. Canak, P., Mirosavljević, M., Ćirić, M., Kešelj, J., Vujošević, B., Stanisavljević, D. and Mitrović, B. 2016. Effect of seed priming on seed vigor and early seedling growth in maize under optimal and suboptimal temperature conditions. Selekcijai Semenarstvo, 22(1): 17-25. [DOI:10.5937/SelSem1601017C]
9. Dadnia, M.R. 2017. Effect of humic acid on activity of antioxidant enzymes and yield of castor bean (Ricinus communis) under water deficit condition. Journal of Crop Ecophysiology, 11(1): 85-98. [In Persian with English Summary].
10. Delian, E., and Lagunovschi-luchian, V. 2015. Germination and vigor of primed Daucus carota L. seeds under saline stress conditions. Romanian Biotechnological Letters, 20(5): 10833-10840.
11. El-Mohamedy, R.S.R. and Ahmed, M.A. 2009. Effect of bio fertilizers and humic acid on control of dry root disease and improvement yield quality of mandarin (Citrus reticulate Blanco). Research Journal of Agriculture and Biological Sciences, 5(2): 127-137.
12. Gebreegziabher, B.G., and Qufa, C.A. 2017. Plant physiological stimulation by seeds salt priming in maize (Zea mays L.): Prospect for salt tolerance. African Journal of Biotechnology, 16(5): 209-223. [DOI:10.5897/AJB2016.15819]
13. Ghaderi Far, F., and Soltani, A. 2012. Seed Control and Certification. Mashhad: Jahade Daneshgahi Mashhad. [In Persian].
14. Ghorbani, S., Khajeh Hosseini, M. and Eshi Rezaei, E. 2013. Effect of pretreatment of humic acid on germination and early seedling growth of maize (Zea mays L.). Journal of Agronomy and Plant Breeding, 9(3): 37-43.
15. Hosseini Khah, F.S., Soheil, P., Tavakkol Afshari, R., Jami Al-Ahmadi, M., and Esmaeili, A.R. 2015. Effects of salicylic acid and gibberellic acid on improvement and prevailing seed deterioration in two sesame (Sesamum indicum L.) cultivars. Iranian Journal of Field Crop Science, 45(4): 613-624. [In Persian with English Summary].
16. ISTA. 2010. International Seed Testing Association, ISTA Handbook on Seedling Evaluation.
17. Izadi, E., Zarghani, H., Mohamadian, M. and Yanegh, A. 2013. Evaluation of germination and seedling growth characteristics of three sesame (Sesamum indicum L.) cultivars in salt and drought stress condition. Agronomy Journal (Pajouhesh and Sazandegi), 102: 92-100. [In Persian with English Summary].
18. Jini, D., and Joseph, B. 2017. Physiological mechanism of salicylic acid for alleviation of salt stress in rice. Rice Science, 24(2): 97-108. [DOI:10.1016/j.rsci.2016.07.007]
19. Jones, C.A., Jacobsen, J.S., and Mugaas, A. 2004. Effects of humic acid on phosphorus availability and spring wheat yield. Facts Fertilizer, 32: 345-352.
20. Karimi, M., and Azizi, M. 2007. Basic Growth Analysis. Mashhad: Jahade Daneshgahi Mashhad. [In Persian].
21. Kiarostami, Kh., Sadri, S.S., Abdolmaleki, N., and Saboora, Az. 2014. Influence of salicylic acid on antioxidant enzyme system in Brassica napus under salt stress. Journal of Applied Biology, 27(2): 85-106. [In Persian with English Summary].
22. Koca, H., Bor, M., Ozdemir, F., and Turkan, I. 2007. The effect of salt stress on lipid peroxidation, anti-oxidative enzymes and proline content of sesame cultivars. Environmental and Experimental Botany, 60: 344-351. [DOI:10.1016/j.envexpbot.2006.12.005]
23. Kolupaev, Y., Yastreb, T., Karpets, Y.V., and Miroshnichenko, N. 2011. Influence of salicylic and succinic acid on antioxidant enzymes activity, heat resistance and productivity of Panicum miliaceum L. Journal of Stress Physiology and Biochemistry, 7: 154-163.
24. Kulkarni, V.V., Sivakumar, K., Singh, A.P., and Visha, P. 2014. Yield and quality characteristics of rendered chicken oil for biodiesel production. Journal Oil Chemistry Sociality, 91: 133-141. [DOI:10.1007/s11746-013-2346-5]
25. Maksymiec, W. 2011. Effects of jasmonate and some other signaling factors on bean and onion growth during the initial phase of cadmium action. Biologia Plantarum, 55(1): 112-118. [DOI:10.1007/s10535-011-0015-9]
26. Moghbeli, T., and Arvin, M.J. 2015. Effect of seed pretreatment on germination parameters, growth, and fruit yield of muskmelon (Cucumis melo L. var. reticulates). Journal of Crop Production and Processing, 4(14): 23-34. [In Persian with English Summary].
27. Mohammad, A., and Rafiei, M. 2011. The effect of different concentrations of humic acid on seed germination behavior and vigor of barley. Journal of Basic and Applied Science, 5(12): 610-613.
28. Moosavi, A., Tavakkol-Afshari, R., SharifZadeh, F., and Aynehband, A. 2009. Effect of seed priming on germination characteristics, polyphenol oxidase, and peroxidase activities of four amaranth cultivars. Journal of Food Agricultural Environmental, 7: 353-358.
29. Parra-Lobato, M.C., Fernandez-Garcia, N., Olmos, E., Alvares-Tinaut, M., and Gomez-Jimenez, C. 2009. Methyl jasmonate induced antioxidant defense in root apoplast from sunflower seedlings. Environmental and Experimental Botany, 66: 9-17. [DOI:10.1016/j.envexpbot.2009.01.002]
30. Safari, Gh., Allah Dadi, I., Arvin, S.M.J., Irannejad, H., Akbari, Gh., and Nemati Qasem Abadi, A. 2012. Effect of some growth regulators on seed germination characters and seedling primary growth of canola (Brassica napus L.). Iranian Journal of Seed Science and Technology, 1(2): 185-192. [In Persian with English Summary].
31. Shashidhara, N., Ravikumar, H., Ashoka, N., Santosh, D.T., Pawar, P., Lokesha, R. and Janagoudar, B.S. 2011. Callus induction and sub-culturing in sesame. International Journal of Agricultural Environmental and Biogeochemistry, 4(3): 153-156.
32. Smith, O.B., Osafo, E.L.K. and Adegbola, A.A. 1988. Studies on the feeding value of agro-industrial by products: strategies for improving the utilization of cocoa-pod-based diets by ruminants. Animal Feed Science Technology, 20: 189-201. [DOI:10.1016/0377-8401(88)90043-0]
33. Soliman, M.H., Al-Juhani, R.S., Hashash, M.A., and Al-Juhani, F.M. 2016. Effect of seed priming with salicylic acid on seed germination and seedling growth of broad bean (Vicia faba L.). International Journal of Agricultural Technology, 12(6): 1125-1138.
34. Tavili, A., Saberi, M., Shahriari, A., and Heidari, M. 2013. Salicylic acid effect on Bromus tomentellus germination and initial growth properties under cadmium stress. Journal of Plant Researches, 26(2): 208-216. [In Persian with English Summary].
35. Tekrony, D.M., and Egli, D.B. 1991. Relationship of seed vigor to crop yield: A review. Crop Science, 31: 816-822. [DOI:10.2135/cropsci1991.0011183X003100030054x]
36. Varier, A., Vari, A.K., and Dadlani, M. 2010. The sub cellular basis of seed priming. Current Science, 99: 450-456.
37. Wani, S.H., Kumar, V., Shriram, V., and Sah, S.K. 2016. Phytohormones and their metabolic engineering for abiotic stress tolerance in crop plants. The Crop Journal, 4: 162-176. [DOI:10.1016/j.cj.2016.01.010]

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