Volume 5, Issue 2 ((Autumn & Winter) 2019)                   Iranian J. Seed Res. 2019, 5(2): 15-28 | Back to browse issues page

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Tabatabaei A, Ansari O. Evaluation of Germination and Biochemical Changes of Two Wheat (Triticum aestivum) Cultivars Under Pb(NO3)2 Stress. Iranian J. Seed Res.. 2019; 5 (2) :15-28
URL: http://yujs.yu.ac.ir/jisr/article-1-247-en.html
Gorgan University of Agricultural Science and Natural Resources , ansari_o@ut.ac.ir
Abstract:   (3581 Views)

DOR: 98.1000/2383-1251.1397.5.

Extended abstract
Introduction: Heavy metal pollution is one of the most serious environmental problems. These metals which accumulate in food chain bring about a lot of hazards to both humans and animals. Among heavy metals, lead is considered to be the most dangerous heavy metal in the environment. It contaminates the environment through the lead-acid battery industry, paint and gasoline additives, insecticides, chemical fertilizers, car exhaust pipes and soldering. The objective of this study was to investigate the effect of Pb(NO3)2 on germination characteristics and biochemical changes of two wheat cultivars (Chamran and Kohdasht cultivars).
Materials and Methods: The objective of this research was to evaluate germination and biochemical changes of two wheat cultivars under Pb(NO3)2 stress, using three-parameter sigmoid model. The experimental design adopted was factorial with a completely randomized design, as the base design, with 3 replications. The first factor was 2 wheat cultivars (Kohdasht and Chamran), and the second factor was 6 levels of Pb(NO3)2 (0, 0.25, 0.5, 0.75, 1 and 1.5 mg.L).
Results: The results showed that with increases in levels of Pb(NO3)2 stress, germination percentage, germination rate, normal seedling percentage, seedling length, seedling weight and seed vigor index reduced for both wheat cultivars. The results of fitting three-parameter sigmoidal to characteristics indicated that the highest characteristics and X50 were obtained from the Chamran cultivar. The highest germination percentage (96%), germination rate (23 seeds per day), normal seedling percentage (93.33%), seedling length (13.07 cm), seedling weight (0.07) and seedling vigor index (12.18) were obtained from the Chamran cultivar under non-stress conditions. Pb(NO3)2 stress increased proline and catalase activity but reduced protein, proline and protein for the Chamran cultivar, as compared with the Kohdasht cultivar.
Conclusion: Generally speaking, the results showed that Pb(NO3)2 had a significant effect on germination characteristics and catalase, proline and protein of wheat. Finally, it could be said that in copper-accumulated areas, choosing proper cultivars can slightly mitigate the damages caused by copper. The Chamran cultivar seems to be a better candidate for these conditions.

  1. Evaluation of the effect of Pb(NO3)2 stress on germination characteristics of wheat.
  2. Using three-parameter sigmoid model for the evaluation of biochemical changes and germination of wheat under Pb(NO3)2 stress.
Full-Text [PDF 478 kb]   (422 Downloads)    
Type of Study: Research | Subject: Seed Ecology
Received: 2018/02/2 | Accepted: 2018/06/23

1. Ansari, O., Choghazardi, H. R., Sharif Zadeh, F., and Nazarli, H. 2012. Seed reserve utilization and seedling growth of treated seeds of mountain rye (Secale montanum) as affected by drought stress. Cercetări Agronomice în Moldova, 45(2): 43-48. [DOI:10.2478/v10298-012-0013-x]
2. Ansari, O., Gherekhloo, J., Kamkar, B., and Ghaderi-Far, F. 2016. Breaking seed dormancy and determining cardinal temperatures for Malva sylvestris using nonlinear regression. Seed Science and Technology, 44(3): 1-14. [DOI:10.15258/sst.2016.44.3.05]
3. Bashmakov, D. I., Lukatkin, A.S., Revin, V.V., Duchovskis, P., Brazaitytë, A., and Baranauskis, K. 2005. Growth of maize seedlings affected by different concentrations of heavy metals. Ekologija, 3: 22-27.
4. Bates, L.S., Waldern, R.P., and Teave, I.D. 1973. Rapid determination of free proline for water stress studies. Plant and Soil, 39: 205-207. [DOI:10.1007/BF00018060]
5. Bhagirath, S., Chauhan, B.S. David, E., and Johnson, M.A.K. 2008. seed germination and seedling Emergence of giant sensitive plant (Mimosa invisa L.). Weed Science, 56(2): 244-248. [DOI:10.1614/WS-07-120.1]
6. Bradford, M. 1976. A rapid and sensitive method for the quantitation of protein utilizing the principle of protein-dye binding. Annual Review of Biochemistry, 72: 248-254. [DOI:10.1016/0003-2697(76)90527-3]
7. Chauhan, B.S., Gill, G., and Preston, C. 2006. Factors affecting seed germination of threehorn bedstraw (Galium tricornatum) in Australia. Weed Science, 54: 471-477. https://doi.org/10.1614/WS-06-060R1.1 https://doi.org/10.1614/WS-05-176R1.1 https://doi.org/10.1614/WS-06-047R.1 https://doi.org/10.1614/WS-06-061R.1 [DOI:10.1614/WS-06-067.1]
8. Cheng, S., and Huang, C. 2006. Influence of cadmium on growth of root vegetable and accumulation of cadmium in the edible root. International Journal of Applied Science and Engineering, 3: 243-252.
9. Cherati Araei, A., and Khanlarian Khatiri, M. 2009. The Effects of Lead on germination, protein and proline contents and index of tolerance in two varieties of oilseed rape (Brassica napus L.). Environmental Sciences, 5(3): 41-52 ]In Persian with English Summary].
10. Cho, U.H., and Park, J.O. 2000. Mercury-induced oxidative stress in tomato seedlings. Plant Science, 156: 1-9. [DOI:10.1016/S0168-9452(00)00227-2]
11. Derakhshan, A., Gherekhloo, J., Vidal, R.B., and De Prado, R. 2013. Quantitative description of the germination of littleseed canarygrass (Phalaris minor) in response to temperature. Weed Science, 62: 250-257. [DOI:10.1614/WS-D-13-00055.1]
12. Diaz, J., Bernal, A., Pomar, F., and Merino, F. 2001. Induction of shikimate dehydrogenase and peroxidase in pepper (Capsicum annuum L.) seedlings in response to copper stress and its relation to lignification. Plant Science, 161(1): 179-188. [DOI:10.1016/S0168-9452(01)00410-1]
13. Eick, M.J., Peak, J.D., Brady P.V., and Pesek J.D. 1999. Kinetics of lead absorption and desorption on goethite: Residence time effect. Soil Science, 164: 28-39. [DOI:10.1097/00010694-199901000-00005]
14. Gajewska, E., and Sklodowska, M. 2007. Effect of nickel on ROS content and oxidative enzyme activities in wheat leaves. Biometals, 20(1): 27-36. [DOI:10.1007/s10534-006-9011-5] [PMID]
15. Janda, T., Szalai, G., Tari I., and Paldi, E. 1999. Hydroponic treatment with salicylic acid decreases the effects of chilling injury in maize (Zea mays L.) plants. Planta, 208: 175-180. [DOI:10.1007/s004250050547]
16. Kabir, M., Iqbal, M.Z., Shafigh, M., and Faroogi, Z.R. 2008.Reduction in germination and seedling growth of Thesp-esiapopulnea L. caused by lead and cadmium treatments. Pakistan Journal of Botany, 40(6): 2419-2426.
17. Kamkar, B., Jami Al-Ahmadi, M., Mahdavi-Damghani, A. and Villalobos, F.J. 2011. Quantification of the cardinal temperatures and thermal time requirement of opium poppy (Papaver somniferum L.) seeds germinate using non-linear regression models. Industrial Crops and Products, 35: 192-198. [DOI:10.1016/j.indcrop.2011.06.033]
18. Knasmuller, S., Gottmann, E., Steinkellner, H., Fomin, A., Pickl, C., Paschke, A., God, R., and Kundi, M. 1998. Detection of genotoxic effects of heavy metal contaminated soils with plant bioassays. Mutation Research, 420: 37-48. [DOI:10.1016/S1383-5718(98)00145-4]
19. Lin, C.J., Liu, L., Liu, T., Zhu, L., Sheng, D., and Wang, D. 2009. Soil amendment application frequency contributes to phytoextraction of lead by sunflower at different nutrient levels. Environmental and Experimental Botany, 65: 410-416. [DOI:10.1016/j.envexpbot.2008.12.003]
20. Molassiotis, A., Satipoulos, T., Tanou, G., Diamantidis, G., and Therios, I. 2005. Boron-induced oxidative damage and antioxidant and nucleolytic responses in shoot tips culture of apple rootstock EM9 (Malus domestica Borkh). Environmental and Experimental Botany, 7: 24-32.
21. Oliver, D., and Naidu, R. 2003. Uptake of Cu, Pb, Cd, As and DDT by vegetables grown in urban environments. Environmental Protection and Heritage Council, 151-161.
22. Pandey, N., and Sharma, C.P. 2002. Effect of heavy metals Co2+, Ni2+ and Cd2+ on growth and metabolism of cabbage. Journal of Plant Science, 163(4): 753-758. [DOI:10.1016/S0168-9452(02)00210-8]
23. Peralta, J.R., Gardea-Torresdey, J.L., Tiemann, K.J., Gomez, E., Arteaga, S., Rascon, E., and Parsons, J. G. 2000. Study of the effects of heavy metals on seed germination and plant growth on alfafa plant (Medicago sativa) growth in solid media. In: Proceedings of the Conference on Hazardous Waste Research, CO. 135-140.
24. Pereira, G. J., Molina, G., and Zevedo, R.A. 2002. Activity of antioxidant enzymes in responses to pb in Crotalaria juncea. Plant and Soil, 239(1): 123-132. [DOI:10.1023/A:1014951524286]
25. Rahman Khan, M., and Mahmud Khan, M. 2010. Effect of varying concentration of nickel and cobalt on the plant growth and yield of chickpea. Australian Journal Basic and Applied Science, 4(6): 1036-1046.
26. Ramezani, F., Shayanfar, A., Tavakkol Afshari, R., and Rashayi, K. 2014. Effects of silver, nickel, zinc and zinc – copper nanoparticles on germination, seedling establishment and enzyme activity of alfalfa (Medicago sativa) seed. Iranian Journal of Field Crop Science, 45(1): 107-118 ]In Persian with English Summary].
27. Saberi, M., Tavili, A., Jafari, M., and Heidari, M. 2010. The effect of heavy metal on germination and seedling growth of Atripex lentiformis. Journal of Rangeland, 4(1): 112-120 ]In Persian with English Summary].
28. Sharma, P.R, and Dubey, S. 2005. Lead toxicity in plants. Brazilian Journal Plant Physiology, 17(1): 35-52. [DOI:10.1590/S1677-04202005000100004]
29. Soltani, A., Gholipoor, M., and Zeinali, E. 2006. Seed reserve utilization and seedling growth of wheat as affected by drought and salinity. Environmental and Experimental Botany, 55: 195-200. [DOI:10.1016/j.envexpbot.2004.10.012]
30. Taghizadeh, M., Kafi, M., Fatahi Moghadam., M.R., and Savaghebi, Gh.R. 2011. Effects of lead concentrations on seed germination of turfgrass genus and its potential for phytoremediation. Iranian Journal of Horticultural Science, 42(3): 277-289 ]In Persian with English Summary].
31. Tavili, A., Saberi, M., Shariari, A., and Heidari, M. 2013. Salicylic acid effect on Bromus tomentellus germination and initial growth properties under cadmium stress. Journal Plant Research (Iranian Journal of Biology), 26(2): 208-216 ]In Persian with English Summary].
32. Van Assche, F., and Clijsters, H. 1990. A biological test system for the evaluation of the phyto-toxicity of metal contaminated soils. Environmental Pollution, 66: 157-172. [DOI:10.1016/0269-7491(90)90118-V]
33. Verma, D.P.S. 1999. Osmotic stress tolerance in plant: Role of proline and sulfur metabolism. In Molecular Responses to Cold, Drought, Heat and Salt Stress in Higher Plant, K. Shinozaki and K. Yamaguchi- shinozaki, (eds). R.G. Landes Company, pp:153-168.
34. Verma, S., and Dubey, R.S. 2001. Effect of cadmium on soluble sugars and enzymes of heir metabolism in rice. Biologia Plantarum, 44(1): 117-123. [DOI:10.1023/A:1017938809311]
35. Walter, I., Martinez, F., and Cala, V. 2006. Heavy metal speciation and phytotoxic effects of three representative sewage sludges for agricultural uses. Environmental Pollution, 139: 507-514. [DOI:10.1016/j.envpol.2005.05.020] [PMID]
36. Xiong, Z.T. 1998. Lead uptake and effects on seed germination and plant growth in a Pb hyper accumulator Brassica pekinensis Rupr. Bulletin of Environmental Contamination and Toxicology, 60(2): 285-291. [DOI:10.1007/s001289900623] [PMID]
37. Yell Yang, Y. 2000. Identification of rice varieties with high tolerance or sensity to lead and characterization of the mechanism of tolerance. Plant Physiology, 124: 1019-1026. [DOI:10.1104/pp.124.3.1019]

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