Volume 3, Issue 1 ((Spring and Summer) 2016)                   Iranian J. Seed Res. 2016, 3(1): 27-37 | Back to browse issues page


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Professor, Faculty of Agriculture, University of Yasouj, Yasouj, Iran , adehdari@yu.ac.ir
Abstract:   (30318 Views)

Medicinal barberry plants, belong to Berberidaceae family. Seeds of barberry have long period dormancy. Therefore, overcome to seed dormancy and increasing germination rate of seeds due to different methods will be useful. In this regard, two separate experiments were designed. In the first experiment, effect of different levels of Gibberellic acid (0, 600 and 900 mg.l-1 at 8˚C) and temperatures (25 (control), 10 and 5 °C at 50 days) in a factorial experiment based on the completely randomized design with three replications and in the second experiment, effect of different nutrition concentrations of MS medium (full strength MS, 1/2MS and 1/4 MS) in a completely randomized design with three replications on embryo culture of four native species of barberry (Berberis integrima, B. vulgaris, B. crataegina and B. orthootrys) were investigated. Based on the results obtained from the first experiment, chilling treated seeds showed the greatest effect (88%) on seed germination in comparison with gibberellic acid treatment. The results of the second experiment showed that the best MS concentration for embryo culture was full strength MS medium with%100 seed germination in four above mentioned barberry species after 2-3 days. In general, the best method to overcome barberry seed dormancy was an embryo culture in full-strength MS nutrition.

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Type of Study: Research | Subject: Seed Physiology
Received: 2015/02/17 | Revised: 2017/12/27 | Accepted: 2015/08/26 | ePublished: 2016/11/9

References
1. Beraak, J.P. 1978. The effect of flowering date and temperature on embryo development in sweet cherry. Netherlands Journal of Agricultural Science, 26: 13-30.
2. Hidayati, S.N., Baskin, J.M., and Baskin, C.C. 2001. Dormancy-breaking and germination requirements for seeds of Symphoricarpos orbiculatus (Caprifoliaceae). American Journal of Botany, 88(8): 1444-1451. [DOI:10.2307/3558452] [PMID]
3. Koornneff, M., Bentsink, L., and Hilhorst, H. 2002. Seed dormancy and germination. Current Opininion in Plant Biology, 5(1): 33-36. [DOI:10.1016/S1369-5266(01)00219-9]
4. Mark, P.B. 1994. A review of plant embryo culture. Horticulturae Science, 29(11): 1243-1246.
5. Murashige, T., and Skoog, F. 1962. A revised medium for rapid growth and bio-assays with tobacco tissue cultures. Physiology of Plant, 15(3): 473-497. [DOI:10.1111/j.1399-3054.1962.tb08052.x]
6. Sakhanokho, H.F., Zipf, A., Rajasekaran, K., Saha, S., and Sharma, G.C. 2001. Induction of highly embryogenic calli and plant regeneration in upland and pima cotton. Crop Science, 41: 1235-1240. [DOI:10.2135/cropsci2001.4141235x]
7. Stanys, V. 1998. Invitro techniques to increase the output of cherry seedling from earlyipening parents. Acta Horticulturae, 468: 203-208. [DOI:10.17660/ActaHortic.1998.468.25]
8. Susan, K., Borwn, A., Iezzoni, A.F., and Harold, W.F. 1996. Cherries spp. Horticulturae Science, 23: 213-225.
9. Tipirdamaz, R., and Gomurgen, N. 2000. The effects of temperature and gibberellic acid on germination of Eranthis hyemalis (L.) salisb seeds. Turkish Journal of Botany, 24(2): 143-145.
10. Vieira, A.R., Vieira, M.G., Fraga, A.C. Oliveira, J.A., and Santos, C.D. 2002. Action of gibberellic acid (GA3) on dormancy and activity of a-amylase in rice seeds. Revista Brasileira de Sementes, 24(2): 43-48. [DOI:10.1590/S0101-31222002000100008]
11. Wei, S., Zhang, C., Chen, X., Li, X., Sui, B., Huang, H., Cui, H., Liu, Y., Zhang, M., and Guo, F. 2010. Rapid and effective methods for breaking seed dormancy in buffalobur (Solanum rostratum). Weed Science, 58(2): 141-146. [DOI:10.1614/WS-D-09-00005.1]

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