Volume 5, Issue 2 ((Autumn & Winter) 2019)                   Iranian J. Seed Res. 2019, 5(2): 91-104 | Back to browse issues page


XML Persian Abstract Print


Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:

Abbasi sourki A, Hosseni Z, Fallah S. Effect of Stratification and Its Combination with Gibberellic Acid on Seed Dormancy Breaking of Echinophora platyloba. Iranian J. Seed Res.. 2019; 5 (2) :91-104
URL: http://yujs.yu.ac.ir/jisr/article-1-203-en.html
, aabasi59@yahoo.com
Abstract:   (3402 Views)

DOR: 98.1000/2383-1251.1397.5. 91.10.2.1575.41

Extended Abstract
Introduction: Seeds are a good option for propagation and protection of medicinal plants. Although Seed dormancy is an adaptive strategy for wild medicinal plants, but it is considered as an undesirable trait in domestication and cultivation of them, need to be solved. Echinophora platyloba seed has dormancy despite its remarkable medicinal properties.
Materials and Methods: In order to break seed dormancy, three separate experiment including stratification, hormonal treatment and combination of both were performed. For stratification 10 samples of were placed in a wet bed at 5 ° C for 2, 4, 6, 8, 10, 12, 14 and 16 Week and were compared using a completely randomized design with three replications. For hormonal treatment, the seeds were placed in GA concentrations of 0, 500 and 1000 ppm for 24 hours and then transferred to germination conditions. However, since the breaking of dormancy did not occur, this experiment was not discussed any more. For combined application of hormone and stratification, seeds were placed at mentioned concentrations of gibberellin for 24 hours at 20 ° C and then gibberellin solutions were removed and the seed transmitted to 5 ° C and compared for 2, 4, 6, 8 weeks with a CRD factorial experiment with three replications. The first factor was concentration of gibberellin in three levels and the second factor was the duration of stratification in 4 levels.
Results: Stratification had a positive effect on seed dormancy breaking and 16 week chilling lead to highest germination percentage and rate and vigor indices. The combined application of hormonal treatments accelerated dormancy release and improved seed germination characteristics, which peaked at 8 weeks. 8-week stratification treatment at 5 ° C with 1000 ppm gibberellic acid was the best treatment to overcoming of dormancy in Echinophora-platyloba seeds.
Conclusion: It seems that seed dormancy of Echinophora seeds is physiological, which broke down by moist chilling and simultaneous application of stratification and gibberellin successfully. Although Gibberellin had no effect on dormancy break, it reduced the need for stratification. Combined application of them showed synergistic effects on dormancy release.
 
 
Highlights:

  1. Echinophora seed’s dormancy was broken under stratification conditions, but GA had no effect on it lonely.
  2. The application of gibberellin reduced the chilling demands of Echinophora seeds and the combined application of them had a synergistic effect on dormancy break
Full-Text [PDF 539 kb]   (399 Downloads)    
Type of Study: Research | Subject: Seed Ecology
Received: 2018/06/15 | Accepted: 2018/11/30

References
1. Aghababanejad, Z. Abbasi Surki, A., and Tahmasebi, P. 2018. Studying interaction of Moist-Chilling and gibberellic acid on germination of Fritillaria imperialis. Iranian Journal of Seed Science and Technology, 6: 257-266 [In Persian with English Summary].
2. Aghababanejad, Z. Tahmasebi, P., and Abbasi Surki, A. 2016. Effect of osmopriming on seed dormancy break and germination parameters of Fritillaria imperialis seed. Iranian Journal of Horticulture Science and Technology, 17: 65-76 [In Persian with English Summary].
3. Amooaghaie, R. 2007. The Effect of Moist-Chilling and GA3 on seed dormancy breaking of Ferula ovina Boiss. Journal of Crop Production and Processing, 11(40): 471-481 [In Persian with English Summary].
4. Bahadory, F., and Javanbakht, A. 2006. Effect of pre-treatments on seed germination and seedling growth of Bunium persicum in Semnan. Iranian Journal of Rangelands and Forests Plant Breeding and Genetic Research, 14: 163-169 [In Persian with English Summary].
5. Baskin, C.C., Meyer, E., and Baskin, J.M. 1995. Two type of morpho-physiological dormancy in seeds of two genera (Osmorhiza and Erythronium) with an Arcto- Tertiary distribution pattern. American Journal of Botany, 82: 293-298. [DOI:10.1002/j.1537-2197.1995.tb12633.x]
6. Bridg, H. 2000. Micro propagation and determination of the in vitro stability of Annona cherimola Mill and Annona muricata L. Humboldt Universitat zu Berlin. 120 p.
7. Cheraghi, F. Mahmoodi, S. Jami Alahmadi, M., and Parsa, S. 2012. Seed germination and growth improvement in Heracleum persicum Desf. by osmo-primming. Journal of Herbal Drugs, 2: 229-238 [In Persian with English Summary].
8. Delaram, M., and Sadeghiyan Z. 2010. The effects of Echinophora platyloba on primary of dysmenorrhea. Arak Medical University Journal 13: 61-67 [In Persian with English Summary].
9. Dhoran V.S., and Gudadhe, S.P. 2012. Effect of plant growth regulators on seed germination and seedling vigour in Asparagus sprengeri Regelin. International Research Journal of Biological Sciences, 1(7):6-10.
10. Ebrahimi R., Hassandokht, M., Zamani, Z. Kashi, A., Roldan-Ruiz, I. and Bockstaele, E.V. 2014. Seed morphogenesis and effect of pretreatments on seed germination of Persian Shallot (Allium hirtifolium Boiss.), an endangered medicinal plant. Horticulture and Environment Biotechnology, 55(1): 19-26. [DOI:10.1007/s13580-014-0032-7]
11. ISTA (International Seed Testing Association). 2009. International Rules for Seed Testing. International seed Testing Association. Bassersdorf. Switzerland.
12. Joosen, R.V., Kodde, J., Willems, L.A., Ligterink, W., Van der Plas, L. H., and Hilhorst, H.W. 2010. GERMINATOR: a software package for high‐throughput scoring and curve fitting of Arabidopsis seed germination. The Plant Journal, 62(1): 148-159. [DOI:10.1111/j.1365-313X.2009.04116.x] [PMID]
13. Kalsa, K.K., and Abebie, B. 2012. Influence of seed priming on seed germination and vigor traits of Vicia villosa ssp. dasycarpa (Ten.). African Journal of Agricultural Research, 7(21): 3202-3208
14. Karsen, C.M., and Lacka, E. 1986. A revision of the hormone balance theory of seed dormancy. Studies on gibberellin and/or abscisic acid deficient mutants of Arabidopsis thaliana. 315-323 in plant growth substances 1985. Berlin, Springer-verlag.
15. Keshtkar, H.R. Azarnivand, H. Etemad, V., and Moosavi, S.S. 2008. Seed dormancy-breaking and germination requirements of Ferula ovina and Ferula gummosa. Desert, 13: 45-51.
16. Khoocheki, A., and Azizi, G. 2005. Effect of different treatments on breaking dormancy of Teucriom polium. Iranian Field crop Research, 3(1): 81-88.[In Persian with English Summary].
17. Koyuncu, F. 2005. Breaking dormancy in black mulberry (Morus nigra L.) by cold stratification and exogenous application of Gibberellic Acid. Acta Biologyca Cracoviencia Botanica, 47(2): 23-26.
18. Mancuso, E., Bedini, G., and Peruzzi, L. 2012. Morphology, germination, and storage behavior in seeds of Tuscan populations of Fritillaria montana (Liliaceae), a rare perennial geophyte in Italy. Turkish Journal of Botany, 36: 161-166.
19. Mozaffarian V. 1996. A dictionary of Iranian plant names: Latin, English, Persian. Farhang Mo'aser Press. 764 p. [In Persian].
20. Nadjafi, F., Bannayan, M., Tabrizi, L., and Rastgoo, M. 2006. Seed germination and dormancy breaking techniques for Ferula gommosa and Teucrium pollium. Journal of Arid Environment, 64(3): 542-547. [DOI:10.1016/j.jaridenv.2005.06.009]
21. Nikolaeva, M.G. 1999. Patterns of seed dormancy and germination as related to plant phylogeny and ecological and geographical condition of their habitats. Russian Journal of Plant Physiology, 46: 432-437.
22. Rahnama-Ghahfarokhi, A., and Tavakol-afshari, R. 2007. Methods for dormancy breaking and germination of Galbanum seeds (Ferula gummosa). Asian Journal Plant Science, 6(4): 611-616. [DOI:10.3923/ajps.2007.611.616]
23. Razavi, S.M. and Hajiboland, R. 2009. Dormancy breaking and germination of Prangos ferulaceae seeds. Eurasian Journal of Bioscience, 3:78-83. [DOI:10.5053/ejobios.2009.3.0.11]
24. Rood, S.B. Buzzel, R.I.L. Major, D.J. and Pharis, R.P. 1990. Gibberellins and heterosis in maize: quantitative relationship. Crop Sciences, 30(2): 281-286. [DOI:10.2135/cropsci1990.0011183X003000020008x]
25. Rouhi, H.R., K. Shakarami, and R. Tavakkol Afshari. 2010. Seed treatments to overcome dormancy of waterlily tulip (Tulipa kaufmanniana Regel.). Australian Journal of Crop Science, 4(9): 718-721.
26. Salehi, A., Masoumi Asl, A., and Moradi, A. 2015. Evaluation of the effective methods of seed dormancy breaking in medicinal plant of Bilhar (Dorema aucheri). Iranian Journal of Seed Research, 2: 66-72 [In Persian with English Summary].
27. Sharifi, M., and Pouresmael, M. 2006. Breaking in seed dormancy in Bunium persicum by stratification and chemical substances. Asian Journal of Plant Science, 5(4): 695-699. [DOI:10.3923/ajps.2006.695.699]
28. Slater, R.J., and Bryant, J.A. 1982. RNA metabolism during breakage if seed dormancy by low temperature treatment of fruits of Acer platanoides. Annals of Botany, 50: 141-149. [DOI:10.1093/oxfordjournals.aob.a086351]
29. Walk J.L., and Hidayati, S.N. 2004. Germination and ecophysiology of the western North American species Osmorhiza depauperata (Apiaceae) implication of predation and phylogenic niche conservatism in seed dormancy evolution. Seed Science Research, 14(4): 387-894. [DOI:10.1079/SSR2004184]
30. Ya-jing, G. Jin H. Xian-ju, W., and Chen-xia, S. 2009. Seed priming with chitosan improves maize germination and seedling growth in relation to physiological changes under low temperature stress. Journal of Zhejiang University Science B, 10(6): 427-433. [DOI:10.1631/jzus.B0820373] [PMID] [PMCID]
31. Zaman, S. 2013. Medicinal Plant. Ghoghnus Press. 368 p. [In Persian].

Add your comments about this article : Your username or Email:
CAPTCHA

Send email to the article author


© 2020 All Rights Reserved | Iranian Journal of Seed Research

Designed & Developed by : Yektaweb

Creative Commons License
This work is licensed under a Creative Commons Attribution 4.0 International License.