Volume 4, Issue 1 ((Spring and Summer) 2017)
Iranian J. Seed Res. 2017, 4(1): 45-59 |
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Darabi F, Valipour M, Naseri , R, Moradi M M. (2017). The Effects of Accelerated Aging Test on Germination and Activity of Antioxidant Enzymes of Maize (Zea mays) Hybrid Varieties Seeds. Iranian J. Seed Res.. 4(1), 45-59. doi:10.29252/yujs.4.1.45
URL: http://yujs.yu.ac.ir/jisr/article-1-252-en.html
URL: http://yujs.yu.ac.ir/jisr/article-1-252-en.html
Ph.D. Student of Plant Growth Physiology, Faculty of Agriculture, Ilam University, Ilam, Iran , m.darabi8161@gmail.com
Abstract: (16251 Views)
Unfavorable storage conditions, especially relatively high environment humidity and high storage temperature greatly affect the quality of corn seeds. The effects of temperature, environment moisture and length of storage on six maize hybrids were examined. For the purpose of investigating germination traits, total soluble proteins, leakage electrolytes and the activity of antioxidant enzymes in maize hybrids, an experiment was carried out at the Agronomy and Plant Breeding Laboratory of Ilam University in 2016. The study was conducted as two factorial experiments, adopting a completely randomized design with three replications. The first factor comprised six maize hybrids (single crosses: 703, 706, 711, 604, Mobin and 701) that were obtained from Karaj Seed Breeding and Seedling Institute, Iran. The second factor was accelerated aging test in four levels involving non-aging (control treatment), aging for 4, 8 and 12 days under 40°C temperature and 95% humidity. The results showed that mean time to germination and electrolyte leakage significantly increased with aging duration. Mean time to germination and electrolyte leakage of the hybrids 701, Mobin and 711 increased more than the other hybrids. In addition, antioxidant enzyme activity decreased significantly with an increase in the aging period. These results indicated severe damage to cell membranes and enzyme activity in these hybrids. Moreover, there was a significant and positive correlation between germination percentage and the enzyme peroxides, as compared with other antioxidant enzymes. Although antioxidant enzyme activity exhibited a significant reduction in seed deterioration, nonetheless, generally speaking, compared with other varieties, KSC 703 was more tolerant.
Highlights:
- The germination response of six hybrids of the maze to seed deterioration was investigated.
- The role of antioxidant enzymes in deteriorated seeds of maize hybrids was examined.
Type of Study: Research |
Subject:
Seed Physiology
Received: 2016/07/26 | Revised: 2018/01/1 | Accepted: 2017/06/13 | ePublished: 2017/12/16
Received: 2016/07/26 | Revised: 2018/01/1 | Accepted: 2017/06/13 | ePublished: 2017/12/16
References
1. Agrawal, R. 2005. Seed technology. Oxford and IBH Publishing Co, 82p.
2. Alscher, R.G. 1989. Biosynthesis and antioxidant function of glutathione in plants. Physiologia Plantarum, 77(3): 457-464. [DOI:10.1111/j.1399-3054.1989.tb05667.x]
3. Bailly, C., Benamar, A., Corbineau, F., and Come, D. 2000. Antioxidant systems in sunflower (Helianthus annuus L.) seeds as affected by priming. Seed Science Research, 10: 35-42. [DOI:10.1017/S0960258500000040]
4. Balesevic-Tubic, S. 2001. The influence of aging process on seed viability and biochemical changes in sunflower seed. Ph.D. Thesis – University of Novi Sad, Novi Sad (Yugoslavia).
5. Basra, S.M.A., Ahmad, N., Khan, M.M., Iqbal, N., and Cheema, M.A. 2003. Assessment of cotton seed deterioration during accelerated ageing. Seed Science and Technology, 31(3): 531-540. [DOI:10.15258/sst.2003.31.3.02]
6. Beauchamp, C., and Fridovich, I. 1971. Superoxide dismutases: improved assays and an assay applicable to acrylamide gels. Analytical Biochemistry 44(1): 276-287. [DOI:10.1016/0003-2697(71)90370-8]
7. Bewley, J.D., and Black, M. 1994. Seeds: Physiology of Development and Germination. Plenum Press, New York. [DOI:10.1007/978-1-4899-1002-8]
8. Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Analytical Biochemistry, 72(1-2): 248-254. [DOI:10.1016/0003-2697(76)90527-3]
9. Chance, B., and Maehly, A.C. 1995. Assay of catalases and peroxidase. Methods in Enzymology, 2: 764-775. [DOI:10.1016/S0076-6879(55)02300-8]
10. Chiu, K.Y., Wang, C.S., and Sung, J.M. 1995. Lipid peroxidation and peroxide-scavenging enzymes associated with accelerated aging and hydration of watermelon seeds differing in ploidy. Physioligia Plantarum, 94: 441-446.
https://doi.org/10.1111/j.1399-3054.1995.tb00951.x [DOI:10.1034/j.1399-3054.1995.940310.x]
11. Demir Kaya, M., Dietz, K.J., and Sivriteoe, H. O. 2010. Changes in antioxidant enzymes during ageing of onion seeds. Notulae Botanicae Horti Agrobotanici Cluj-Napoca, 38(1): 49-52.
12. Gholami Tilebeni, H., and Golpayegani, A. 2011. Effect of seed ageing on physiological and biochemical changes in rice seed (Oryza sativa L.). International Journal of AgriScience, 1(3): 138-143.
13. Goel, A., Goel, A.K., and Sheoran, I.S. 2003. Changes in oxidative stress enzymes during artificial ageing in cotton (Gossypium hirsutum L.) seeds. Journal of Plant Physiology, 160: 1093-1100. [DOI:10.1078/0176-1617-00881] [PMID]
14. Gupta, A., and Aneja, K.R. 2004. Seed deterioration in soybean varieties during storage-physiological attributes. Seed Research, 32(1): 26-32.
15. ISTA, 1999. International rules for seed testing. Seed Science and Technology, 13: 299-520.
16. Kalpana, R., and Madhava Rao, K.V. 1994. Absence of the role of lipid peroxidation during accelerated ageing of seeds of pigeon pea (Cajanus cajan L.) cultivars. Seed Science and Technology, 22(2): 253-260.
17. Kibinza, S., Vinel, D., Coˆ me, D., Bailly, C., and Corbineau, F. 2006. Sunflower seed deterioration as related to moisture content during ageing, energy metabolism and active oxygen species scavenging. Physiologia Plantarum, 128(3): 496-506. [DOI:10.1111/j.1399-3054.2006.00771.x]
18. Lehner, A., Mamadou, N., Poels, P., Come, D., Bailly, C., and Corbineau, F. 2008. Change in soluble carbohydrates, lipid peroxidation and antioxidant enzyme activityes in the embryo during aging in wheat grains. Journal of Cereal Science, 47(3): 555-565. [DOI:10.1016/j.jcs.2007.06.017]
19. Linn, S.S., and Pearce, R.S. 1990. Changes in lipids in bean seeds (Phaseolus vulgaris) and corn caryopses (Zea mays) aged in contrasting environments. Annals of Botany, 65(4): 451–456. [DOI:10.1093/oxfordjournals.aob.a087955]
20. McDonald, M.B. 1999. Seed deterioration: physiology, repair, and assessment. Seed Science and Technology, 27: 177-237.
21. Mohammadi, H., Soltani, A., Sadeghipour, H.R., and Zeinali, E. 2011.Effect of seed aging on subsequent seed reserve utilization and seedling growth in soybean. International Journal of Plant Production, 5(1): 65-70.
22. Mohsennasab, F., Sharifizadeh, M., and Siadat A. 2010. Study the Seed deterioration on germination and seedling growth of wheat genotype under laboratory conditions. Journal of Crop Physiology, 7: 59-71.
23. Msuya, D.G., and Stefano, J. 2010. Responses of maize (Zea mays L.) seed germination capacity and vigour to seed selection based on size of cob and selective threshing. World Journal of Agricultural Sciences, 6(6): 683-688.
24. Priestly, D.A. 1986. Seed aging: Implication for seed storage and persistence in the soil. Cornell University Press. Ithaca, NY. [PMCID]
25. Pukacka, S., and Ratajczak, E. 2005. Production and scavenging of reactive oxygen species in Fagussylvatica seeds during storage at varied temperature and humidity. Journal of Plant Physiology, 162: 873-885. [DOI:10.1016/j.jplph.2004.10.012] [PMID]
26. Rajjou, L., Lovigny, Y., Job, C., Belghazi, M., Groot, S., and Job, D. 2007. Seed quality and germination. In Seeds: Biology, Development and Ecology (eds.). 324-332. [DOI:10.1079/9781845931971.0324]
27. Rao, R.G.S., Singh, P.M., and Mathura, R. 2006. Storability of onion seeds and effects of packaging and storage conditions on viability and vigour. Scientia Horticulturae, 110: 1-6. [DOI:10.1016/j.scienta.2006.06.002]
28. Sinha, A.K. 1972. Colorimetric assay of catalase. Analytical Biochemistry, 47(2): 389-394. [DOI:10.1016/0003-2697(72)90132-7]
29. Soltani, E., Galeshi, S., Kamkar, B., and Akramghaderi, F. 2008. Modeling seed aging effects on response of germination to temperature in wheat. Seed Science and Biotechnology, 2(1): 32-36.
30. Sung, J.M. 1996. Lipid peroxidation and peroxide-scavenging in soybean seeds during aging. Physiologia Plantarum, 97(1): 85-89.
https://doi.org/10.1034/j.1399-3054.1996.970113.x [DOI:10.1111/j.1399-3054.1996.tb00482.x]
31. Sung, J.M., and Jeng, T.L. 1994. Lipid peroxidation and peroxide-scavenging enzymes associated with accelerated aging of peanut seed. Physiologia Plantarum, 91(1): 51-55.
https://doi.org/10.1111/j.1399-3054.1994.tb00658.x [DOI:10.1034/j.1399-3054.1994.910108.x]
32. Tammela, P., Vaananen, P.S., Lakso, I., Hopia, A., Vourela, H., and Nygrem, M. 2005. Tocopherols, tocoterienols and fatty acids as indicators of natural ageing in Pinus syluestris seeds. Scandinavian Journal of Forest Research, 20(5): 378-384. [DOI:10.1080/02827580500292063]
33. Tatić, M., Balešević-Tubić, S., Vujaković, M. and Nikolić, Z. 2008. Changes of germination during natural and accelerated aging of soybean seed. Proc. The Second Joint PSU–UNS International Conference on BioScience: Food, Agriculture and the Environment Jun 22–24, 2008 Novi Sad, Serbia.
34. Walters, C. 1998. Understanding the mechanisms and kinetics of seed aging. Seed Science Research, 8(2): 223-244. [DOI:10.1017/S096025850000413X]
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