Volume 3, Issue 2 ((Autumn & Winter) 2017)                   Iranian J. Seed Res. 2017, 3(2): 57-70 | Back to browse issues page

XML Persian Abstract Print

Download citation:
BibTeX | RIS | EndNote | Medlars | ProCite | Reference Manager | RefWorks
Send citation to:
Sarami R, Omidi H, Bostani A A. (2017). The Effect of Auxin and Cytokinin on Some Morpho-Physiological and Germination Characteristics of Stevia (Stevia rebaudiana Bertoni) Seeds. Iranian J. Seed Res.. 3(2), 57-70. doi:10.29252/yujs.3.2.57
URL: http://yujs.yu.ac.ir/jisr/article-1-190-en.html
The Effect of Auxin and Cytokinin on Some Morpho-Physiological and Germination Characteristics of Stevia (Stevia rebaudiana Bertoni) Seeds
Razieh Sarami , Heshmat Omidi , Abdol amir Bostani
, Agricultural College and Medicinal Plant Research Center, Shahed University Tehran, Tehran, Iran , omidi@shahed.ac.ir
Abstract:   (19036 Views)

The present study was conducted to investigate the efficiency of hormonal pretreatment on increasing germination and early growth of seedling in the Seed Technology Laboratory of Shahed University in 2015 as a factorial experiment, adopting a completely randomized design with 4 replications. The treatments were: 5 levels of IAA (indole-3-acetic acid) namely, zero, 0.1, 0.5, 1 and 1.5 mg/L and 5 levels of PBA (Tetrahydro pyranyl ­benzyl­ adenine), which were zero, 0.5, 1, 1.5 and 2 mg/L. Analysis of variance showed that the use of the two hormones and their interactions had a significant effect on all traits such as seed germination, biomass, leaf relative water content and photosynthetic pigments. The highest percentage of germination (66.66%) was obtained in 1.5 mg/L IAA with 1 mg/L PBA. 0.5 mg/L PBA and in 0.1 mg/L IAA the longest shoot (1.28 and 1.17 cm, respectively) was obtained. Germination coefficient decreased by about 12.5% by increasing IAA from 0.1 to 1.5 mg/L. The greatest relative water content (42.73% and 37.38%) was obtained with 0.5 mg/L PBA and 1.5 mg/L IAA, respectively. Combination of IAA+PBA (0.1+0 mg/L) had a positive effect on both the length of the root and seedling. The high concentration of PBA and the lowest amount of IAA had similar results in terms of plant biomass. The highest plant biomass (4.33 mg) was obtained in seeds treated with 2 mg/L of cytokinin and 0.1 mg/L of auxin. Photosynthetic pigments were also affected by these two hormones through the IAA was more effective than PBA. The finding was that auxin and cytokinin increase germination and improve the morpho-physiological indicators and thus increase the yield of Stevia.
 

Keywords: Indole-3-acetic acid, Tetra ‌hydro pyranyl ‌benzyl ‌adenine, Germination coefficient, Biomass
Full-Text [PDF 485 kb]   (3148 Downloads)    
Type of Study: Research | Subject: Seed Physiology
Received: 2016/01/17 | Revised: 2017/12/28 | Accepted: 2016/06/26 | ePublished: 2017/05/13
References
1. Arnon, D.I. 1949. Copper enzymes in isolated chloroplasts, polyphenoxidase in Beta vulgaris. Plant Physiology, 24(1): 1-15. [DOI:10.1104/pp.24.1.1] [PMID] [PMCID]
2. Ayub, M., Ibrahim, M., Noorka, I.R., Tahir, M., Tanveer, A., and Ullah, A. 2013. Effect of seed priming on seed germination and seedling growth of garden cress (Lepidium sativum L.). International Journal of Agriculture and Applied Sciences, 5(2): 1-5.
3. Azizi, P., Rafii, M.Y., Maziah, M., Abdullah, S.N.A., Hanafi, M.M., Latif, M.A., Rashid, A.A., and Sahebi, M. 2015. Understanding the shoot apical meristem regulation: A study of the phytohormones, auxin and cytokinin, in rice. Mechanisms of Development, 135: 1-15. [DOI:10.1016/j.mod.2014.11.001] [PMID]
4. Delker, C., Zolman, B.K., Miersch, O., and Wasternack, C. 2007. Jasmonate biosynthesis in Arabidopsis thaliana requires peroxisomal β-oxidation enzymes–Additional proof by properties of pex6 and aim1. Phytochemistry, 68(12): 1642-1650. [DOI:10.1016/j.phytochem.2007.04.024] [PMID]
5. Deshmukh, S.H., and Ade, R. 2012. In vitro rapid multiplication of Stevia rebaudiana: an important natural sweetener herb. Bioscience, 4(3): 105-108.
6. El-Araby, M.M., and Hegazi, A. Z. 2004. Responses of tomato seeds to hydro- and osomo- priming and possible relations of some antioxidant enzyme and endogenous polyamine fractions. Egyptian Journal of Biology, 6(1): 81-93.
7. Gajdosova, S., Spichal, L., Kaminek, M., Hoyerova, K., Novak, O., Dobrev, P.I., and Motyka, V. 2011. Distribution, biological activities, metabolism, and the conceivable function of cis-zeatin-type cytokinins in plants. Journal of Experimental Botany, 62: 2827-2840. [DOI:10.1093/jxb/erq457] [PMID]
8. Ghorbani‌Javid, M., Sorooshzadeh, A., Moradi, F., Sanavy, S.A.M.M., and Allahdadi, I. 2011. The role of phytohormones in alleviating salt stress in crop plants. Australian Journal of Crop Science, 5(6): 726-734.
9. Ibrahim, I.A., Nasr, M.I., Mohammed, B.R., and El-Zefzafi, M.M. 2008. Plant growth regulators affecting in vitro cultivation of Stevia rebaudiana. Sugar Technology, 10(3): 254-259. https://doi.org/10.1007/s12355-008-0044-7 [DOI:10.1007/s12355-008-0045-6]
10. ISTA. 2010. International rules for seed testing. International seed testing association (ISTA). 543: 1-53.
11. Karuppusamy, S. 2009. A review on trends in production of secondary metabolites from higher plants by in vitro tissue, organ and cell cultures. Journal of Medicinal Plants, 3(13): 1222-1239.
12. Kieber, J.J., and Schaller, G.E. 2014. Cytokinins. In the Arabidopsis Book. Kumar, R., Sharma, S., and Sood, S. 2014. Yield components, light interception and marker compound accumulation of stevia (Stevia rebaudiana Bertoni) affected by planting material and plant density under western Himalayan conditions. Archives of Agronomy and Soil Science, 60(12): 1731-1745.
13. Miransari, M., and Smith, D.L. 2014. Plant hormones and seed germination. Environmental and Experimental Botany, 99: 110-121. [DOI:10.1016/j.envexpbot.2013.11.005]
14. Pol, J., Ostra, E. V., Karasek, P., Roth, M., Benesova, K., Kotlarlkova, P. and Caslavsky, J. 2007. Comparison of two different solvents employed for pressurised fluid extraction of stevioside from Stevia rebaudiana: methanol versus water. Analytical and Bioanalytical Chemistry, 388(8): 1847-1857. [DOI:10.1007/s00216-007-1404-y] [PMID]
15. Qasim, M., Ashraf, M.M., Jamil, A.M., Rehman, Y.S.U., and Rha, E.S. 2003. Water relations and gas exchange properties in some elite canola (Brassica napus L.) lines under salt stress. Annual Application of Biology, 142(3): 307-316. [DOI:10.1111/j.1744-7348.2003.tb00255.x]
16. Raina, R., Bh, S.K., and Sharma, Y. 2013. Strategies to improve poor seed germination in Stevia rebaudiana, a low calorie sweetener. Journal of Medicinal Plants Research, 7(24): 1793-1799.
17. Ramesh, K., Singh, V., and Megeji, N.W. 2006. Cultivation of stevia [Stevia rebaudiana (Bertoni)]: A comprehensive review. Advances in Agronomy, 89: 137-177. [DOI:10.1016/S0065-2113(05)89003-0]
18. Razak, U. N.A.A., Ong, C.B., Yu, T.S., and Lau, L.K. 2014. In vitro micropropagation of Stevia rebaudiana Bertoni in Malaysia. Brazilian Archives of Biology and Technology, 57(1): 23-28. [DOI:10.1590/S1516-89132014000100004]
19. Reis, M., Coelho, L., Santos, G., Kienle, U., and Beltrão, J. 2015. Yield response of stevia (Stevia rebaudiana Bertoni) to the salinity of irrigation water. Agricultural Water Management, 152: 217-221. [DOI:10.1016/j.agwat.2015.01.017]
20. Su, Y.H., Liu, Y.B., and Zhang, X.S. 2011. Auxin–cytokinin interaction regulates meristem development. Molecular Plant, 4(4): 616-625. [DOI:10.1093/mp/ssr007] [PMID] [PMCID]
21. Vamil, R., Aniat-ul-haq, R., Agnihotri, K., and Sharma, R. 2011. Effect of certain plant growth regulators on the seedling survival, biomass production and proline content of Bambusa arundinacea. Science Research Reporter, 1(2): 44-48.
22. Vanneste, S., and Friml, J. 2009. Auxin: a trigger for change in plant development. Cell, 136(6): 1005-1016. Vejsadova, H. 2006. Factors affecting seed germination and seedling growth of Terrestrial Orchids cultured in vitro. Acta Biologica Cracoviensia Series Botanica, 48: 109-113.
23. Woodward, A.W., and Bartel, B. 2005. Auxin: regulation, action, and interaction. Annals of Botany, 95(5): 707-735. [DOI:10.1093/aob/mci083] [PMID] [PMCID]
24. Yakimova, E., Kapchina, V., Groshkoff, I., and Ivanova, G. 2000. Effects of BA and CPPU on protease and α-amylase activity of in vitro cultured explants of Rosa hybrida L. Bulgarian Journal of Plant Physiology, 26(1-2): 39-47.
25. Zeng, J., Chen, A., Li, D., Yi, B., and Wu, W. 2013. Effects of salt stress on the growth, physiological responses, and glycoside contents of Stevia rebaudiana Bertoni. Journal of Agricultural and Food Chemistry, 61(24): 5720-5726. [DOI:10.1021/jf401237x] [PMID]
26. Zhao, Y. 2010. Auxin biosynthesis and its role in plant development. Annual Review of Plant Biology, 61: 49-64. [DOI:10.1146/annurev-arplant-042809-112308] [PMID] [PMCID]
Add your comments about this article : Your username or Email:
CAPTCHA
Send email to the article author

Rights and permissions
Creative Commons License This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.

© 2024 CC BY-NC 4.0 | Iranian Journal of Seed Research

Designed & Developed by : Yektaweb


This work is licensed under a Creative Commons Attribution 4.0 International License.