Volume 11, Issue 2 ((Autumn & Winter) 2025)
Iranian J. Seed Res. 2025, 11(2): 19-40 |
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Hamidi A, Oskuoei B, Shayanfar A. (2025). Study on optimal temperature, light, duration, and substrate conditions for three Salicornia species seed germination. Iranian J. Seed Res.. 11(2), 19-40. doi:10.61882/yujs.11.2.19
URL: http://yujs.yu.ac.ir/jisr/article-1-617-en.html
URL: http://yujs.yu.ac.ir/jisr/article-1-617-en.html
Agriculture Research, Education and Extension Organization(AREO), Seed and Plant Certification and Registration Institute (SPCRI) , a.hamidi@areeo.ac.ir
Abstract: (1430 Views)
Extended abstract
Introduction: Salicornia is a halophyte plant which cultivation is important for reclamation of saline soils and producing fodder. Seed germination has always been of interest to plant ecologists due to its key role in plant population establishment. Also, due to the importance of this process in seed certification, this phenomenon is of interest to control and seed certification experts. Temperature, access to sufficient humidity, and the presence of light in light-sensitive species for seed germination are considered to be the most important natural factors for seed germination. Additionally, the time required for germination and sufficient early seedling growth are important to determine the potential seed germination. Therefore, determining the temperature, the need or lack of light, as well as the time required for germination and the suitable substrate for planting seeds, are of great importance in the process of seed certification laboratory tests. This experiment was conducted to investigate the optimal conditions of temperature, light, duration, and substrate for seed germination of three Salicornia species.
Materials and Methods: A preliminary study was conducted to determine the light requirements, duration, and suitable substrate for the standard germination test of Salicornia persica, S. persepolitana, and S. bigelovi, seeds. Since no difference was observed in the percentage of seedlings emerging in light and darkness (seeds of the studied Salicornia species germinated under light and dark conditions) and maximum seed germination was achieved within 7 and 12 days in the substrate between germination paper (BP) and top of paper (TP), at constant temperatures of 20°C and 25 °C and alternating temperatures of 20-25 °C (8-16 hours/day-night), the main experiment was carried out under these conditions.
Results: The results showed that the seeds of S. bigelovi species had the highest percentage of normal seedlings at 25 °C constant temperature for 7 days in the top-of-paper (TP) substrate. Also, the seeds of S. persica had the highest percentage of normal seedlings at 20-25 °C alternating temperature for 7 days in the top-of-paper (TP) substrate. S. persepolitana seeds at 25 °C constant temperature for 7 days on the top of paper (TP) substrate had the highest percentage of normal seedlings. S. persica, S. bigelovi, and S. persepolitana seeds had a higher percentage of normal seedlings in both germination durations and temperatures, respectively.
Conclusions: The results of the research showed that the seeds of the studied Salicornia species did not require light for germination. Also, the studied Salicornia species in the research had significant differences in terms of temperature, duration, and optimal substrate for the standard germination test. So that the optimal temperature for germination of S. persica seeds was alternative temperature and the optimal temperature for germination of S. bigelovii and S. perspolitana seeds were constant temperature. The constant temperature for germination of S. bigelovii species seeds was higher than the constant temperature for germination of S. perspolitana seeds. Also, the top of paper (TP) substrate was suitable for the standard germination test of all three species.
Highlights:
Introduction: Salicornia is a halophyte plant which cultivation is important for reclamation of saline soils and producing fodder. Seed germination has always been of interest to plant ecologists due to its key role in plant population establishment. Also, due to the importance of this process in seed certification, this phenomenon is of interest to control and seed certification experts. Temperature, access to sufficient humidity, and the presence of light in light-sensitive species for seed germination are considered to be the most important natural factors for seed germination. Additionally, the time required for germination and sufficient early seedling growth are important to determine the potential seed germination. Therefore, determining the temperature, the need or lack of light, as well as the time required for germination and the suitable substrate for planting seeds, are of great importance in the process of seed certification laboratory tests. This experiment was conducted to investigate the optimal conditions of temperature, light, duration, and substrate for seed germination of three Salicornia species.
Materials and Methods: A preliminary study was conducted to determine the light requirements, duration, and suitable substrate for the standard germination test of Salicornia persica, S. persepolitana, and S. bigelovi, seeds. Since no difference was observed in the percentage of seedlings emerging in light and darkness (seeds of the studied Salicornia species germinated under light and dark conditions) and maximum seed germination was achieved within 7 and 12 days in the substrate between germination paper (BP) and top of paper (TP), at constant temperatures of 20°C and 25 °C and alternating temperatures of 20-25 °C (8-16 hours/day-night), the main experiment was carried out under these conditions.
Results: The results showed that the seeds of S. bigelovi species had the highest percentage of normal seedlings at 25 °C constant temperature for 7 days in the top-of-paper (TP) substrate. Also, the seeds of S. persica had the highest percentage of normal seedlings at 20-25 °C alternating temperature for 7 days in the top-of-paper (TP) substrate. S. persepolitana seeds at 25 °C constant temperature for 7 days on the top of paper (TP) substrate had the highest percentage of normal seedlings. S. persica, S. bigelovi, and S. persepolitana seeds had a higher percentage of normal seedlings in both germination durations and temperatures, respectively.
Conclusions: The results of the research showed that the seeds of the studied Salicornia species did not require light for germination. Also, the studied Salicornia species in the research had significant differences in terms of temperature, duration, and optimal substrate for the standard germination test. So that the optimal temperature for germination of S. persica seeds was alternative temperature and the optimal temperature for germination of S. bigelovii and S. perspolitana seeds were constant temperature. The constant temperature for germination of S. bigelovii species seeds was higher than the constant temperature for germination of S. perspolitana seeds. Also, the top of paper (TP) substrate was suitable for the standard germination test of all three species.
Highlights:
- Light was not necessary for the studied Salicornia species seeds' germination.
- The germination response of the seeds of the studied Salicornia species to the optimum germination temperature and duration varied.
- The studied Salicornia species did not differ significantly in terms of suitable growing medium for seed germination.
Keywords: Constant and alternating temperatures, Germination duration, Germination substrate, Native Salicornia species
Type of Study: Research |
Subject:
General
Received: 2024/05/29 | Revised: 2024/11/2 | Accepted: 2024/11/3 | ePublished: 2025/03/19
Received: 2024/05/29 | Revised: 2024/11/2 | Accepted: 2024/11/3 | ePublished: 2025/03/19
References
1. Aghaleh, M., Niknam, V., Ebrahimzadeh, H. and Razavi, K. 2009. Salt stress effects on growth, pigments, proteins and lipid peroxidation in Salicornia persica and S. europaea. Biologia Plantarum, 53(2): 243-248. [DOI:10.1007/s10535-009-0046-7]
2. Ajmal Khan, M., Gul, B. and Weber, D.J. 2000. Germination responses of Salicornia rubra to temperature and salinity. Journal of Arid Environments, 45: 207-214. [DOI:10.1006/jare.2000.0640]
3. Akhani, H. 2003: Salicornia persica Akhani (Chenopodiaceae) a remarkable new species from central Iran. Linzer Biologische Beiträge, 35(1): 607-612.
4. Akhani, H. 2008. Taxonomic revision of the genus Salicornia L. (Chenopodiaceae) in central and southern of Iran. Pakistan Journal of Botany, 40(4): 1635-1655.
5. Akhani, H., 2006. Biodiversity of halophytic and sabkha ecosystems in Iran: 71-88. In: Khan, M.A., Böer, B., Kust, G.S. and Barth, H.-J., (Eds.). Sabkha Ecosystems. Volume II: West and Central Asia. Springer, 263p. [DOI:10.1007/978-1-4020-5072-5_6]
6. Balouchi, H., Soltani Khankahdani, V., Moradi, A., Gholamhoseini, M., Piri, R., Heydari, S.Z. and Dedicova, B. 2023. Seed fatty acid changes germination response to temperature and water potentials in six sesame (Sesamum indicum L.) cultivars: Estimating the cardinal temperatures. Agriculture, 13(10): 1-17. [DOI:10.3390/agriculture13101936]
7. Baskin, C.C. 2003. Breaking physical dormancy in seeds - focussing on the lens. New Phytologist, 158(2): 229-232. [DOI:10.1046/j.1469-8137.2003.00751.x]
8. Baskin, J.M. and Baskin, C.C., 2014. Seeds: Ecology, Biogeography, and Evolution of Dormancy and Germination. Academic Press, San Diego, CA, USA.
9. Benvenuti, S., Macchia, M., and Miele, S. 2001. Quantitative analysis of emergence of seedlings from buried weed seeds with increasing soil depth. Weed Science, 49: 528-535. [DOI:10.1614/0043-1745(2001)049[0528:QAOEOS]2.0.CO;2]
10. Boer, B. 1997. An introduction to the climate of the United Arab Emirates. Journal of Arid Environments, 35: 3-16. [DOI:10.1006/jare.1996.0162]
11. Calone, R., Sanoubar, R., Noli, E. and Barbanti, L. 2020. Assessing Salicornia europaea tolerance to salinity at seed germination stage. Agriculture, 10(29):1-11. [DOI:10.3390/agriculture10020029]
12. Cárdenas-Pérez, S., Piernik, A., Chanona-Pérez, J.J., Grigore, M.N. and Perea-Flores, M.J. 2021. An overview of the emerging trends of the Salicornia L. genus as a sustainable crop. Environmental and Experimental Botany, 191: 104606. [DOI:10.1016/j.envexpbot.2021.104606]
13. Copeland, L.O. and Mc Donald, M.B., 2004. Principles of Seed Science and Technology. Springer, Dordrecht, Netherlands.
14. De Melo, P.A.F.R., M. Id. P. Cavalcanti, E. U. Alves, C. C. Martins and L. R. de Araújo, 2017. Substrates and temperatures in the germination of Eriotheca gracilipes seeds. Revista Ciência Agronômica, 48(2): 303-309. [DOI:10.5935/1806-6690.20170035]
15. Don, R. and Ducournau, S. 2018. ISTA Handbook on Seedling Evaluation Fourth Edition. International Seed Testing Association (ISTA), Zürichstr. 50, 8303 Bassersdorf, Switzerland.
16. El-Keblawy A Gairola, S., Bhatt, A. and Mahmoud, T. 2017. Effects of maternal salinity on salt tolerance during germination of Suaeda aegyptiaca: a facultative halophyte in the Arab Gulf desert. Plant Species Biology, 32: 45-53. [DOI:10.1111/1442-1984.12127]
17. El-Keblawy, A. and Bhatt, A. 2015. Aerial seed bank affects germination behavior of two small seeded halophytes in the Arabian deserts. Journal of Arid Environments, 117: 10-17. [DOI:10.1016/j.jaridenv.2015.02.001]
18. El-Keblawy, A., Al-Shamsi, N. and Mosa, K. 2018. Effect of maternal habitat, temperature and light on germination and salt tolerance of Suaeda vermiculata, a habitat in different halophyte of arid Arabian deserts. Seed Science Research, 28: 140-147. [DOI:10.1017/S0960258518000144]
19. Ellias, S.G., Copeland, L.O., McDonald, M.B. and Baalbaki, R.Z. 2012. Seed Testing. Michigan State University Press.
20. FAO. 2011. Proceedings of the global forum on salinization and climate change (GFSCC2010). Rome, Italy: Food and Agriculture Organization of the United Nations, 110 pp.
21. Franklin, K.A. and Whitelam, G.C., 2005. Phytochromes and shade-avoidance responses in plants. Annals of Botany, 96: 169-175. [DOI:10.1093/aob/mci165] [PMID] []
22. García-Galindo, E., Nieto-Garibay, A., Troyo-Diéguez, E., Lucero-Vega, G., Murillo-Amador, B., Ruiz-Espinoza, F.H. and Fraga-Palomino, H.C. 2021. Germination of Salicornia bigelovii (Torr.) under shrimp culture effluents and the application of vermicompost leachate for mitigating salt stress. Agronomy, 11(424): 1-15. [DOI:10.3390/agronomy11030424]
23. Guan, B., Zhou, D., Zhang, H., Tian, Japhet, Y., W. and Wang, P. 2009. Germination responses of Medicago ruthenica seeds to salinity, alkalinity, and temperature. Journal of Arid Environment, 73: 135-138. [DOI:10.1016/j.jaridenv.2008.08.009]
24. Gul, B., Khan, M.A. and Weber, D.J. 2000. Alleviation salinity and dark-enforced dormancy in Allenrolfea occidentalis seeds under various thermo periods. Australian Journal of Botany, 48: 745-752. [DOI:10.1071/BT99069]
25. Holeski, L.M., Jander, G. and Agrawal, A.A. 2012. Transgenerational defense induction and epigenetic inheritance in plants. Trends in Ecology & Evolution, 27: 618-626. [DOI:10.1016/j.tree.2012.07.011] [PMID]
26. International Seed Testing Association (ISTA), 2022. International Rules for Seed Testing. International Seed Testing Association, Zürich, Switzerland.
27. Khan, M. A. Gul, B. and Weber, D. J. 2000. Germination responses of Salicornia rubra to temperature and salinity. Journal of Arid Environments, 45: 207-214. [DOI:10.1006/jare.2000.0640]
28. Khan, M.A. and Gul, B. 1998. High salt tolerance in the germinating dimorphic seeds of Arhrocnemum macrostachyum. International Journal of Plant Sciences, 159: 826-832. [DOI:10.1086/297603]
29. Khan, M.A. and Weber, D.J. 1986. Factors influencing seed germination in Salicorniapacifica var. utahensis. American Journal of Botany, 73: 1163-1167. [DOI:10.1002/j.1537-2197.1986.tb08562.x]
30. Khan, M.A., Gul, B., 2006. Halophyte seed germination. In: Khan, M.A., Weber, D.J. (Eds.), Ecophysiology of High Salinity Tolerant Plants. Springer, the Netherlands, pp. 11-30. [DOI:10.1007/1-4020-4018-0_2]
31. Khan, M.A., Gul, B., Weber, D.J., 2001. Seed germination characteristics of Halogeton glomeratus. Canadian Journal of Botany, 79: 1189-1194. [DOI:10.1139/b01-097]
32. Lee, S.J., Jeon, H-J., Jeong, J-H. and Chung, N-J. 2016. Germination is enhanced by removal of the Funiculus in the Halophyte Glasswort (Salicornia herbacea). Horticulture, Environment, and Biotechnology, 57(4): 323-329. [DOI:10.1007/s13580-016-0108-7]
33. Moriuchi KS., Friesen, M.L., Cordeiro, M.A., Badri, M., Vu, W.T., Main, B.J., Elarbi Aouani, M., Nuzhdin, S.V., Strauss, S.Y. and von Wettberg, E.J.B. 2016. Salinity adaptation and the contribution of parental environmental effects in Medicago truncatula. PloS One, 11(3): 1-19. [DOI:10.1371/journal.pone.0150350] [PMID] []
34. Nouri Akandi, Z., Goshasbi, F. and Mahforouzi, R. 2021. Study of seed germination and seedling growth of Salicornia species in different concentrations of sodium chloride. Chemistry Proceeding, 3.
35. Orlovsky, N., Japakova, U., Zhang, H. and Volis, S. 2016. Effect of salinity on seed germination, growth and ion content in dimorphic seeds of Salicornia europaea L. (Chenopodiaceae). Plant Diversity, 38: 183-189. [DOI:10.1016/j.pld.2016.06.005] [PMID] []
36. Oveisi, M., Alizadeh, H., Lorestani, S.A., Esmaili, A., Sadeghnejad, N., Piri, R., Gonzalez-Andujar, J.L. and Müller-Schärer, H. 2024. Triangle area model (TAM) for predicting germination: An approach to enhance hydrothermal time model applications. Current Plant Biology, 39: 1-15. [DOI:10.1016/j.cpb.2024.100356]
37. Patel, S. 2016. Salicornia: Evaluating the halophytic extremophile as a food and a pharmaceutical candidate. 3 Biotech, 6(104): 1-10. [DOI:10.1007/s13205-016-0418-6] [PMID] []
38. Ranal, M. and De Santana, D.G. 2006. How and why to measure the germination process? Revista Brasilian Botanique, 29(1): 1-11 [DOI:10.1590/S0100-84042006000100002]
39. Rueda-Puente, E.O., Garcı'a-Hernández, J.L., Preciado-Rangel, P., Murillo-Amador, B., Tarazón-Herrera, M.A., Flores-Herna'ndez, A. Holguin-Peña, J., Aybar, A.N., Barro'nHoyos, J.M., Weimers, D., Mwandemele, O., Kaaya, G., Mayoral, J.L. and Troyo-Die'guez, E. 2007. Germination of Salicornia bigelovii ecotypes under stressing conditions of temperature and salinity and ameliorative effects of plant growth-promoting bacteria. Journal of Agronomy and Crop Science, 193: 167-176. [DOI:10.1111/j.1439-037X.2007.00254.x]
40. Singh, D., Buhmann, A.K., Flowers, T.J., Seal, C.E. and Papenbrock, J. 2014. Salicornia as a crop plant in temperate regions: selection of genetically characterized ecotypes and optimization of their cultivation conditions AoB Plants, 6: 1-20. [DOI:10.1093/aobpla/plu071] [PMID] []
41. Soltani, A. and Maddah, V. 2010. Simple, Applied Programs for Education and Research in Agronomy, Shahid Beheshti University Press. Tehran, Iran. [In Persian]
42. Ventura, V. and Sagi, M. 2013. Halophyte crop cultivation: The case for Salicornia and Sarcocornia. Environmental and Experimental Botany, 92:144-153. [DOI:10.1016/j.envexpbot.2012.07.010]
43. Zerai, D.B., Glenn, E.P., Chatervedi, R., Lu, Z., Mamood, A.N., Nelson, S.G. and Ray, D.T. 2010. Potential for the improvement of Salicornia bigelovii through selective breeding. Ecological Engineering, 36: 730-739. [DOI:10.1016/j.ecoleng.2010.01.002]
44. Zia, S. and Khan, M.A. 2004. Effect of light, salinity, and temperature on seed germination of Limonium stocksii. Canadian Journal of Botany, 82(2): 151-157. [DOI:10.1139/b03-118]
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