Volume 10, Issue 2 ((Autumn & Winter) 2024)
Iranian J. Seed Res. 2024, 10(2): 137-150 |
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Majdi M, Tavakkol Afshari R, Khazaee H R, Mirshamsi Kakhki A. (2024). The effects of heat stress on germination and growth of tomato (Solanum lycopersicum) pollen grains under laboratory conditions. Iranian J. Seed Res.. 10(2), : 9 doi:10.61186/yujs.10.2.137
URL: http://yujs.yu.ac.ir/jisr/article-1-600-en.html
URL: http://yujs.yu.ac.ir/jisr/article-1-600-en.html
Ferdowsi Univ. of Mashhad , tavakolafshari@um.ac.ir
Abstract: (872 Views)
Extended abstract
Introduction: The effects of temperature increases on the growth of tomato fields are among the obvious results of global warming and are considered an important issue that should be investigated. To maintain and develop the cultivation systems of this crop, a proper understanding of the heat tolerance mechanisms and physiological responses in tomatoes should be achieved. The primary objective of this research is to discover the impact of heat stress on the germination and growth of pollen grains in research tomato germplasms. The researchers' knowledge about the response of different tomato cultivars to abiotic stresses is limited and only the effects of enzymes involved in the response process, heat shock proteins and some hormones have been investigated. The process of detecting heat stress-sensitive stages and their enhancement is facilitated by having a correct understanding of physiological processes.
Materials and methods: The seeds of heat-resistant (LA2661 and LA2662) and -sensitive (LA3911) research cultivars of tomato were used to evaluate the effects of increasing day and night temperatures. The obtained seedlings were grown under optimal temperature conditions (24°C day/18°C night), and after observing the first flower primordium, were incubated in growth chambers to apply daytime heat stress treatments, including temperatures of 28°C, 32°C and 36°C day/18°C night and night stress treatments including temperatures of 28°C, 32°C, and 36°C at night/ 24°C day for 7 days. Pollen grains were then evaluated for their survival, germination, and growth.
Results: The findings of the daytime heat stress tests show that the percentage of survival and germination of pollen grains and growth of pollen tubes of cultivars LA2661, LA2662 and LA3911 decreased as daytime temperature rose from 24°C to 36°C. This reduction is more noticeable for the sensitive cultivar LA3911. Degraded pollen grains increased in the LA3911 cultivar due to heat stress. The survival percentage of pollen grains in all three studied cultivars decreased due to the application of heat stress at night. The resistant cultivars LA2661 and LA2662 had a higher germination percentage compared to the sensitive cultivar LA3911. Pollen grains germination decreased by 50% as a result of increasing the night temperature from 18°C to 36°C. Pollen tube length was reduced in both cultivars and night treatments.
Conclusion: The effects of heat stress in the early stages of flowering when flowers are visible are high, and reproductive stages are very sensitive to high temperatures and affect fertility and processes after insemination, and finally, they lead to yield loss. The daytime temperature increase relative to the natural temperature range (22°C to 24°C) during growth severely impacts the number of pollen grains released from tomato flowers. The number of non-living pollen grains is higher at 36°C day and 32°C and 36°C night temperatures compared to optimal temperature conditions. It appears that the increase in nighttime temperature results in more severe consequences than the increase in daytime temperature.
Highlights:
Introduction: The effects of temperature increases on the growth of tomato fields are among the obvious results of global warming and are considered an important issue that should be investigated. To maintain and develop the cultivation systems of this crop, a proper understanding of the heat tolerance mechanisms and physiological responses in tomatoes should be achieved. The primary objective of this research is to discover the impact of heat stress on the germination and growth of pollen grains in research tomato germplasms. The researchers' knowledge about the response of different tomato cultivars to abiotic stresses is limited and only the effects of enzymes involved in the response process, heat shock proteins and some hormones have been investigated. The process of detecting heat stress-sensitive stages and their enhancement is facilitated by having a correct understanding of physiological processes.
Materials and methods: The seeds of heat-resistant (LA2661 and LA2662) and -sensitive (LA3911) research cultivars of tomato were used to evaluate the effects of increasing day and night temperatures. The obtained seedlings were grown under optimal temperature conditions (24°C day/18°C night), and after observing the first flower primordium, were incubated in growth chambers to apply daytime heat stress treatments, including temperatures of 28°C, 32°C and 36°C day/18°C night and night stress treatments including temperatures of 28°C, 32°C, and 36°C at night/ 24°C day for 7 days. Pollen grains were then evaluated for their survival, germination, and growth.
Results: The findings of the daytime heat stress tests show that the percentage of survival and germination of pollen grains and growth of pollen tubes of cultivars LA2661, LA2662 and LA3911 decreased as daytime temperature rose from 24°C to 36°C. This reduction is more noticeable for the sensitive cultivar LA3911. Degraded pollen grains increased in the LA3911 cultivar due to heat stress. The survival percentage of pollen grains in all three studied cultivars decreased due to the application of heat stress at night. The resistant cultivars LA2661 and LA2662 had a higher germination percentage compared to the sensitive cultivar LA3911. Pollen grains germination decreased by 50% as a result of increasing the night temperature from 18°C to 36°C. Pollen tube length was reduced in both cultivars and night treatments.
Conclusion: The effects of heat stress in the early stages of flowering when flowers are visible are high, and reproductive stages are very sensitive to high temperatures and affect fertility and processes after insemination, and finally, they lead to yield loss. The daytime temperature increase relative to the natural temperature range (22°C to 24°C) during growth severely impacts the number of pollen grains released from tomato flowers. The number of non-living pollen grains is higher at 36°C day and 32°C and 36°C night temperatures compared to optimal temperature conditions. It appears that the increase in nighttime temperature results in more severe consequences than the increase in daytime temperature.
Highlights:
- Night heat stress was assessed as a factor that influences the germination and survival of tomato pollen grains.
- Image analysis was used to measure the length of the pollen tube.
- The effect of thermal stress on pollination was investigated during a specific period of reproductive growth.
Article number: 9
Type of Study: Research |
Subject:
Seed Physiology
Received: 2024/01/27 | Revised: 2024/06/9 | Accepted: 2024/02/14 | ePublished: 2024/06/9
Received: 2024/01/27 | Revised: 2024/06/9 | Accepted: 2024/02/14 | ePublished: 2024/06/9
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