Volume 9, Issue 2 ((Autumn & Winter) 2023)
Iranian J. Seed Res. 2023, 9(2): 17-34 |
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Barahouei M, Moosavi S G, Seghatoleslami M J, Baradaran R, Javadzadeh S M. (2023). Effect of seed priming on some germination indices and enzymatic activity of safflower (Carthamus tinctorius) seedling under drought stress in vitro. Iranian J. Seed Res.. 9(2), : 2 doi:10.61186/yujs.9.2.17
URL: http://yujs.yu.ac.ir/jisr/article-1-548-en.html
URL: http://yujs.yu.ac.ir/jisr/article-1-548-en.html
Mansoor Barahouei 
, Seyyed Gholamreza Moosavi , Mohamad Javad Seghatoleslami , Reza Baradaran , Seyyed Mahdi Javadzadeh
, Seyyed Gholamreza Moosavi , Mohamad Javad Seghatoleslami , Reza Baradaran , Seyyed Mahdi Javadzadeh
Birjand Branch, Islamic Azad University , moosavi@iaubir.ac.ir
Abstract: (2676 Views)
Extended Abstract
Introduction: Safflower is a plant that has been considered due to its high medicinal and nutritional value, especially in the extraction of edible oils in developed countries. Drought is one of the most important harmful factors in arid and semi-arid regions of the world that affects plant production. Modifiers play an important role in plant adaptation to stress conditions. Among these compounds are the hormone gibberellic acid and the antioxidant ascorbic acid, which increase plant tolerance to adverse environmental conditions. The present study investigated the effect of gibberellic acid and ascorbic acid on seed germination parameters and some enzymatic indices of safflower under drought stress.
Materials and Methods: The experiment was conducted as a factorial based on a completely randomized design with three replications in the Agricultural Science Laboratory of Iranshahr University in 2020. Experimental treatments included three levels of control (pretreatment with distilled water), pretreatment with gibberellic acid and ascorbic acid, and four levels of drought stress (0, -3, -6, and -9 bar). Drought stress was applied using polyethylene glycol 6000. Seed germination was carried out inside a germinator at 25 ° C for 14 days in darkness. Germination traits and enzymatic indices were measured using standard methods.
Results: The results of variance showed that most germination and growth indices of safflower seedlings decreased with increasing drought stress. Also, drought stress led to changes in the activity of antioxidant enzymes. Seed priming with gibberellic acid and ascorbic acid increased germination indices and seedling growth and improved enzymatic activity, including catalase, peroxidase, and superoxide dismutase in comparison with untreated seeds. Priming with gibberellic acid had a significant advantage. Seed priming in drought stress conditions has increased germination rate, protein content, and catalase, peroxidase, and ascorbic dismutase activity, respectively, compared to the control.
Conclusion: In general, seed priming of safflower using gibberellic acid changed the activity of antioxidant enzymes. These activities ultimately moderated the negative effects of drought stress and increased germination parameters.
Highlights:
Introduction: Safflower is a plant that has been considered due to its high medicinal and nutritional value, especially in the extraction of edible oils in developed countries. Drought is one of the most important harmful factors in arid and semi-arid regions of the world that affects plant production. Modifiers play an important role in plant adaptation to stress conditions. Among these compounds are the hormone gibberellic acid and the antioxidant ascorbic acid, which increase plant tolerance to adverse environmental conditions. The present study investigated the effect of gibberellic acid and ascorbic acid on seed germination parameters and some enzymatic indices of safflower under drought stress.
Materials and Methods: The experiment was conducted as a factorial based on a completely randomized design with three replications in the Agricultural Science Laboratory of Iranshahr University in 2020. Experimental treatments included three levels of control (pretreatment with distilled water), pretreatment with gibberellic acid and ascorbic acid, and four levels of drought stress (0, -3, -6, and -9 bar). Drought stress was applied using polyethylene glycol 6000. Seed germination was carried out inside a germinator at 25 ° C for 14 days in darkness. Germination traits and enzymatic indices were measured using standard methods.
Results: The results of variance showed that most germination and growth indices of safflower seedlings decreased with increasing drought stress. Also, drought stress led to changes in the activity of antioxidant enzymes. Seed priming with gibberellic acid and ascorbic acid increased germination indices and seedling growth and improved enzymatic activity, including catalase, peroxidase, and superoxide dismutase in comparison with untreated seeds. Priming with gibberellic acid had a significant advantage. Seed priming in drought stress conditions has increased germination rate, protein content, and catalase, peroxidase, and ascorbic dismutase activity, respectively, compared to the control.
Conclusion: In general, seed priming of safflower using gibberellic acid changed the activity of antioxidant enzymes. These activities ultimately moderated the negative effects of drought stress and increased germination parameters.
Highlights:
- The role of gibberellic acid and ascorbic acid on safflower seed germination traits was investigated.
Article number: 2
Type of Study: Research |
Subject:
General
Received: 2021/12/17 | Revised: 2024/02/21 | Accepted: 2022/04/24 | ePublished: 2023/06/14
Received: 2021/12/17 | Revised: 2024/02/21 | Accepted: 2022/04/24 | ePublished: 2023/06/14
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