Volume 10, Issue 1 ((Spring and Summer) 2023)                   Iranian J. Seed Res. 2023, 10(1): 195-208 | Back to browse issues page


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Khalaj H. (2023). Evaluation of dormancy breaking and germination improvement methods in Althaea officinalis. Iranian J. Seed Res.. 10(1), : 12 doi:10.61186/yujs.10.1.195
URL: http://yujs.yu.ac.ir/jisr/article-1-499-en.html
Payame Noor University, tehran- shahriar , h.khalaj@pnu.ac.ir
Abstract:   (668 Views)
Extended Abstract
Introduction: Althaea officinalis L. is one of the most important plants of the Malvaceae family which is used in traditional medicine and as a drug to treat the disorders of digestive and respiratory systems. The fresh seeds of Althaea do not have a good growth potential. This experiment was performed to evaluate the different methods of seed dormancy breaking on the improvement of A. officinalis L. seed germination.
Materials and Methods: An experiment was conducted in a completely randomized design with three replications at the agricultural laboratory of Payame Noor University Tehran, Shahriar Center in 2017. The experimental treatments included 10 treatments (control, seed coat removal, seed coat removal + gibberellic acid (500 and 1000 ppm), seed coat removal + potassium nitrate (0.1 and 0.2%), seed coat removal + sulfuric acid (30 and 60 minutes), sulfuric acid (30 and 60 minutes).
Results: The results showed that the highest germination index with averages of 433.3 was observed in seed coat removal + 30- minutes of sulfuric acid treatment. The highest germination rate (44.7 seed/day) was observed in seed coat removal + 60-minute sulfuric acid treatment. The highest germination percentage (86.6%) was observed in seed coat removal+ 0.2% potassium nitrate treatment. Also, the highest mean germination time (20.2 day) was observed in both control and seeds coat removal treatments. The highest plumule and seedling length and fresh and dry weight were observed in seed coat removal + 30 and 60- minute sulfuric acid, and seed coat removal +0.2% potassium nitrate treatment, without significant difference. The highest radicle length was obtained in seed coat removal +30- minutes sulfuric acid treatment and seed coat removal + 0.2% potassium nitrate treatments.
Conclusion: Since all three sulfuric acid, potassium nitrate and gibberellic acid treatment along with seed coat removal treatment significantly affect the measured traits compared with control. It may be suggested that A. officinalis L. seed has a type of physical and physiological dormancy and seed dormancy breaking treatments (especially 30- minutes sulfuric acid) can be used to increase germination the fresh seeds of this plant.

Highlights:
  1. In A. officinalis plant, removal of seed coat using chemical treatments is very effective in applying seed dormancy treatments.
 Development of A. officinalis seed cultivation and propagation is possible by applying seed dormancy breaking methods.
Article number: 12
Full-Text [PDF 437 kb]   (192 Downloads)    
Type of Study: Research | Subject: Seed Physiology
Received: 2021/04/18 | Revised: 2024/02/21 | Accepted: 2021/10/3 | ePublished: 2023/11/26

References
1. Amoaghaei, R. 2007. The effect of GA3 and moist-Chilling on seed dormancy breaking of Ferula ovina Boiss. Isfahan University of Technology-Journal of Crop Production and Processing, 11(40): 471-482.
2. Arteca, R.N. 2013. Plant growth substances: principles and applications. Springer Science and Business Media.
3. Aydin, I. and Uzun, F. 2001. The effects of some applications on germination rate of Gelemen Clover seeds gathered from natural vegetation in Samsun. Pakistan Journal of Biological Sciences, 4(2): 181-183. [DOI:10.3923/pjbs.2001.181.183]
4. Azizi, H., Rezvani moghadam, P., Parsa, M., Shur, M. and Khorasani, R. 2019. Evaluation of dormancy breaking treatments and some seed germination characteristics of Colchicum kotschyi Boiss. Iranian Seed Science and Research, 6(1): 399-410. [In Persian with English Summary]
5. Baiat, M., Rahmani, A., Amir Nia, R., and Ramezani, M. 2015. The effect of priming and duration of priming on germination indices and seedling characteristics of Althaea officinalis L. Iranian Seed Science and Technology Journal. 4(1):73-82. [In Persian with English Summary]
6. Bakhshande Faraj Pour, A., Dehghani Bidgoli, R. and Hoseini Tafreshi, A. 2019. Effects of potassium nitrate (KNO3) on germination and some morphophysiological characteristics of Astragalus hamosus in MS plate. Journal of Seed Research, 9(1): 23-33. [In Persian with English Summary]
7. Baskin, C.C. and Baskin, J.M. 2014. Seeds: ecology, biogeography and evolution of dormancy and germination. (2nd ed.) (Academic Press: San Diego, CA, USA).
8. Baskin, J.M. and Baskin, C.C. 2004. A classification system for seed dormancy. Seed Science Research, 14(1): 1-16. https://doi.org/10.1017/S0960258518000417 https://doi.org/10.1079/SSR2003150 https://doi.org/10.1017/S0960258520000367 [DOI:10.1017/S0960258515000033]
9. Bonaterra, G.A., Bronischewski, K., Hunold, P., Schwarzbach, H., Heinrich, E.U., Fink, C. and Kinscherf, R. 2020. Anti-inflammatory and anti-oxidative effects of phytohustil and root extract of Althaea officinalis L. on Macrophages in vitro. Frontiers in Pharmacology, 11: 290. [DOI:10.3389/fphar.2020.00290] [PMID] []
10. Bradford, K. and Nonogaki, H. 2008. Annual plant reviews, seed development, dormancy and germination. John Wiley & Sons publisher. [DOI:10.1002/9780470988848]
11. Çetinbaş, M. and Koyuncu, F. 2006. Improving germination of Prunus avium L. seeds by gibberellic acid, potassium nitrate and thiourea. Horticulture Science, 33(3): 119-123. [DOI:10.17221/3750-HORTSCI]
12. Ehyaee, M.R. and Khaje Hoseini, M. 2011. Evaluation of germination and dormancy characteristics in 30 seed masses of medicinal plants. Iranian Journal of Crop Research, 9(4): 651-658. [In Persian with English Summary]
13. Foster, S. 1991. The species: identification and distribution. In Echinacea, Nature's Immune Enhancer. 107-115.
14. Ghadamyari, S., Mozafari, J., Sokhandan, B. N., Mosavi, L. and Rakhshande ru, F. 2011. Synergistic effects of mechanical and chemical treatments on seed germination of Jimsonweed (Datura stramonium L.). Iranian Journal Biology, 24(6): 809-817. [In Persian with English Summary]
15. Ghaderi, A., Kamkar, B. and Soltani, A. 2008. Plant Physiology. Publication of Jahad e Daneshgahi. [In Persian]
16. Ghasemi Pirbalouti, A., Golparvar, A. R., Riyahi Dehkordi, M., and Navid, A. R. 2007. The effect of different treatments on seeds dormancy and germination of five species of medicinal plants of Chahar mahal and Bakhteyari province. Pajouhesh and Sazandegi, 20(1): 185-192. [In Persian with English Summary]
17. Gholami, Sh., Amini Deh Haghi, M. and Ahmadi, Kh. 2019. Evaluation of the effect of some priming and duration treatments on germination characteristics of cumin (Cuminum cyminum). Journal of Seed Research, 9(1): 1-11. [In Persian with English Summary]
18. Giba, Z., Grubišić, D., Todorović, S., Sajc, L., Stojaković, Đ. and Konjević, R. 1998. Effect of nitric oxide-releasing compounds on phytochrome-controlled germination of Empress Tree seeds. Plant Growth Regulation, 26(3): 175-181. [DOI:10.1023/A:1006131215297]
19. Gonzalez, S. and Condón, F. 2020. Germination of Bromus auleticus after different treatments to release seed dormancy. Seed Science and Technology, 48(1): 27-31. [DOI:10.15258/sst.2020.48.1.04]
20. Hojati, Y., Naderi, R. A., Faramarzi, A. and Gholoi Pour, J. 2007. Evaluation of the effect of sulfuric acid, gibberellic and temperature treatments on seed germination of Cycas revolute L. Journal of Modern Agricultural Knowledge, 3(9): 14-22. [In Persian with English Summary]
21. Kaneko, M., Itoh, H., Ueguchi-Tanaka, M., Ashikari, M. and Matsuoka, M. 2002. The α-amylase induction in endosperm during rice seed germination is caused by gibberellin synthesized in epithelium. Plant Physiology, 128(4): 1264-1270. [DOI:10.1104/pp.010785] [PMID] []
22. Kaya, M. D., Okçu, G., Atak, M., Cıkılı, Y. and Kolsarıcı, Ö. 2006. Seed treatments to overcome salt and drought stress during germination in sunflower (Helianthus annuus L.). European Journal of Agronomy, 24(4): 291-295. [DOI:10.1016/j.eja.2005.08.001]
23. Kermode, A. R.2005. Role of abscisic acid in seed dormancy. Journal of Plant Growth Regulation, 24(4): 319-344. [DOI:10.1007/s00344-005-0110-2]
24. Khaje hoseini, M., Rashed mohasel, M. H., Mahmudi, P. and Emami pour, Y. 2018. Study of germination and seed dormancy characteristics of fourteen species of medicinal plants in Kerman (Mint family). Iranian Journal of Seed Science and Technology, 1: 233-242. [In Persian with English Summary]
25. Maguire, J.D. 1962. Speed of germination-Aid in selection and evaluation for seedling emergence and vigor 1. Crop Science, 2(2): 176-177. [DOI:10.2135/cropsci1962.0011183X000200020033x]
26. Mahmudi, J. and Naseri, B. 2019. Investigation of the effect of some physical and chemical treatments on breaking dormancy and germination of Robinia pseudoacacia L. seed. Journal of Seed Research, 9(2): 52-60. [In Persian with English Summary]
27. Mehrabi, A. A. and Haji nia, S. 2019. The effect of seed pretreatments on improving seed germination of white Astragalus (Astragalus gossypinus). Iranian Seed Research, 6(1): 95-113 [in Persian]. [DOI:10.29252/yujs.6.1.95]
28. Mokhtari, M. and Falah, S. 2019. The effect of gibberellin and salicylic acid on the tolerance of Cucurbita pepo seedling to cold stress. Iranian Journal of Seed Research, 6(1): 159-172. [In Persian with English Summary] [DOI:10.29252/yujs.6.1.159]
29. Muhammad, A.J., Atif, A., Mazhar, M.Z., Tanvir, A., Zia, B., Anmbreen, I., Zohaib Anjum, M. and Shabir Mahr, M. 2020. Effect of different pre-treatments on seed germination of Prosopis juliflora and Dalbergia sissoo: a step towards mutation breeding. Journal of Forest Science, 66(2): 80-87. [DOI:10.17221/64/2019-JFS]
30. Nesi, C.N., De Arruda, G.O. and Menegatti, A. 2016. Dormancy overcoming in jatobá seeds assessed by survival analysis. Revista de Ciências Agroveterinárias, 15(1): 42-49. [DOI:10.5965/223811711512016042]
31. Nokes, J. 2001. How to grow native plants of Texas and the Southwest. University of Texas Press.
32. Norouzi Haroni, N. and Tabari Kouchsaraei, M. 2014. The effect of hydropriming, halopriming and boiling water on seed germination of black locust (Robinia pesudoacasia L.). Ecology of Iranian Forest, 2(3): 76-88. [In Persian with English Summary]
33. Olmez, Z. and Gokturk, A. 2009. Effects of cold stratification, sulphuric acid, submersion in hot and tap water pretreatments in the greenhouse and open field conditions on germination of bladder-Senna (Colutea armena Boiss. and Huet.) seeds. African Journal of Biotechnology, 8: 2973-2977.
34. Panahi, F. and Arast, M. 2019. Investigation of different methods of stimulating germination and dormancy breaking on Gundelia tournefortii seeds. Iranian Seed Science and Research, 6(3): 347-358 [in Persian].
35. Patade, V.Y., Bhargava, S. and Suprasanna, P. 2009. Halo priming imparts tolerance to salt and PEG induced drought stress in sugarcane. Agriculture, Ecosystems and Environment, 134(1): 24-28. [DOI:10.1016/j.agee.2009.07.003]
36. Radsarian, Z., Karamshahi, A. A., Mirzaee, J. and Heidari, M. 2017. The effect of different chemical and physical treatments on germination of Crataegus pontica C. Koch. Iranian Seed Research Sciences, 4(4):1-12. [In Persian with English Summary]
37. Ranal, M.A., Santana, D.G., Ferreira, W.R. and Mendes-Rodrigues, C. 2009. Calculating germination measurements and organizing spreadsheets. Brazilian Journal of Botany, 32: 849-855. [DOI:10.1590/S0100-84042009000400022]
38. Rostami Pour, A., Moradi, A., esavand, H.R. and Nasiri, M. 2015. Investigation of seed dormancy and the most suitable methods for three rangeland ecotypes (Astragalus cyclophyllus). Iranian Journal of Seed Science and Technology, 4(2): 51-65. [In Persian with English Summary]
39. Sari, F.A., Ghamari Zare, A., Shahr zad, Sh., Naderi shahab, M.A. and Kalate jari, S. 2011. The effect of different physicochemical treatments on seed dormancy of Salvia leriifolia Benth. Iranian Journal of Medicinal and Aromatic Plants Research, 27(4): 659-667. [In Persian with English Summary]
40. Shah, R.M., Rahman, N.A., Nawi, I.H., Idris, N.I.M. and Soh, N.C. 2020. The evaluation of sexual and vegetative propagation of medicinal plant Christia vespertilionis (Butterfly Wing Plant). In 5th International Conference on Food, Agriculture and Natural Resources, Atlantis Press. 210-212. [DOI:10.2991/aer.k.200325.040]
41. Shamsi, F., Roshandel, P. and Kharazian, N. 2015. The effect of different treatments on dormancy breaking and stimulation of seed germination of Atriplex leucoclada Boiss. Journal of Plant Research, 28(5): 1043-1053. [In Persian with English Summary]
42. Sharifi, H., Khaje hoseini, M. and Rashed ohasel, M. H. 2016. The effect of mechanical scarification on dormancy and improved seed germination of 12 species of medicinal plants. Journal of Seed Research, 6(1):11-18. [In Persian with English Summary]
43. Sikhondze, T., Nxumalo, K.A., Masarirambi, M.T., Wahome, P.K. and Zwane, M.G. 2020. The effects of pre-germination treatments on seed germination and growth of Wild Guavas in the Kingdom of Eswatini, Southern Africa. Asian Journal of Agricultural and Horticultural Research, 5(1): 28-36. [DOI:10.9734/ajahr/2020/v5i130041]
44. Soltani, A., Zeinali, E., Galeshi, S. and Latifi, N. 2001. Genetic variation for and interrelationships among seed vigor traits in wheat from the Caspian Sea coast of Iran. Seed Science and Technology, 29(3): 653-662. [In Persian with English Summary]
45. Soltanipour, M.A., Asadpour, A. and Bagheri, R. 2011. Study of pre-treatments on seed germination of Zygophyllum atriplicoides. Environmental Erosion Researches, 1(2): 69-82. [In Persian with English Summary]
46. Tavili, A., Abasi Khalaki, M. and Moamari, M. 2012. The effect of different methods of dormancy breaking on seed germination and some characteristics of Astragalus. Iranian Journal of Seed Science and Technology, 1(1): 64-72. [In Persian with English Summary]
47. Yadav, S. and Sinha, A. 2020. Breaking of dormancy in seeds of Citrullas Lanatus Thumb. and Cucumis Melo L. due to different treatments. Studies in Indian Place Names, 40(50): 4311-4321.
48. Yazdani byuki, R. and Rezvani Moghadam, P. 2012. Investigation of germination characteristics of Althea officinalis L. under drought and salinity stress. Iranian Journal of Crop Research, 10(1): 1-10. [In Persian with English Summary]
49. Yousif, M.A.I., Wang, Y.R. and Dali, C. 2020. Seed dormancy overcoming and seed coat structure change in Leucaena leucocephala and Acacia nilotica. Forest Science and Technology, 16(1):1-8. [DOI:10.1080/21580103.2019.1700832]
50. Zarafshan, M., Tairi Kuchak Raee, M., Satarian, A. and Baiat, D. 2012. The effect of gibberellic acid and sulfuric acid on germination traits of Celtis australis L. Journal of Plant and Ecology, 8(30): 19-37. [In Persian with English Summary]
51. Zargari, A. 1996. Medicinal Plants Volume III. 5th edition. University of Tehran, 500-501. [In Persian]

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