Volume 6, Issue 2 ((Autumn & Winter) 2020)                   Iranian J. Seed Res. 2020, 6(2): 45-60 | Back to browse issues page

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Malek M, Ghaderi-Far F, Torabi B, Sadeghipour H R. The Effect of Priming on Seed Viability of Canola (Brassica napus) Cultivars under Different Storage Conditions. Iranian J. Seed Res.. 2020; 6 (2) :45-60
URL: http://yujs.yu.ac.ir/jisr/article-1-409-en.html
Gorgan University of Agricultural Sciences and Natural Resources , farshidghaderifar@gau.ac.ir
Abstract:   (5800 Views)

Extended Abstract
Introduction: Priming is one of the most commonly used seed enhancement techniques. Events such as increased synthesis of nucleic acids, activation of repair processes, increased respiratory activity, and improved antioxidant capacity during priming lead to advanced metabolism in seeds. The most important effects of priming include increased percentage, speed and uniformity of germination and emergence. However, the longevity of primed seeds in storage is the major concern for researchers as it restricts widespread use of this technique. Some researchers believe that priming reduces the storage capacity of seeds, while others have reported increased seed shelf life after using priming treatments. Therefore, this study sought to investigate the effects of priming on the storage capacity of the seeds of canola cultivars under different storage conditions.
Material and Methods: In this study, the effects of priming on the shelf life of seeds of three canola cultivars including Dk-xpower, Traper and Hayola50 were investigated. For this purpose, the seeds were first treated with hydropriming and osmopriming methods. Then primed and control seeds with 6, 9, 12 and 15% moisture content were stored for 8 months at 15, 25, 35 and 45 °C. Sampling from different seed treatments was carried out at intervals of 1 to 30 days to assess germination. Finally, by fitting a three-parameter logistic model to cumulative germination data versus the day after storage, the time to germination loss to 50% was calculated and used to compare seed storage behavior between the treatments.
Results: The results showed that the storage behavior of canola seed varies greatly depending on the cultivar, and each cultivar showed a distinct behavior. Priming effects on the shelf life of seeds were different depending on the storage conditions, cultivars and also the priming methods. Comparison of the effects of priming on the seeds’ shelf life under different storage conditions showed that priming treatments were more efficient under higher seed moisture content and storage temperatures than those with lower seed moisture content and storage temperatures. In addition, priming treatments in Dk-xpower cultivar often increased the seeds’ shelf life. However, in the Traper and Hayola 50 cultivars, hydropriming often improved the seeds’ shelf life, and in contrast to osmopriming, it led to a decrease in the shelf life of the seeds.
Conclusion: Based on the results of this study, it was shown that priming effects on canola seed viability can be a function of various factors such as cultivar, storage conditions, and also the type of priming treatment. Moreover, in this study, hydropriming often increased seed longevity whereas osmopriming often increased the deterioration rate and reduced seed longevity.
  1. Seed storage behavior of canola cultivars was compared under natural storage conditions.
  2. Priming effects on seed longevity of canola cultivars was investigated under different storage conditions.
Article number: 4
Full-Text [PDF 450 kb]   (743 Downloads)    
Type of Study: Research | Subject: Seed Physiology
Received: 2019/03/2 | Accepted: 2019/06/10

1. Akram-Ghaderi, F., Soltani, E., Soltani A. and Miri, A.A. 2008. Effect of priming on response of germination to temperature in cotton. Journal of Agriculture Science and Natural Resources, 15: 44-51. [In Persian with English Summery].
2. Alivand, R., Tavakkol Afshari, R. and Sharifzadeh, F. 2013. Investigation of rapeseed (Brassica napus) seed germination and forecasting of seed deterioration under different storage conditions. Iranian Journal of Field Crop Science, 44: 69-83. [In Persian with English Summery]
3. Argerich, C.A., Bradford, K.J. and Tarquis, A.M. 1989. The effects of priming and ageing on resistance to deterioration of tomato seeds. Journal of Experimental Botany, 40(5): 593-598. https://doi.org/10.1093/jxb/40.5.599 [DOI:10.1093/jxb/40.5.593]
4. Aymen, E.M. and Hannachi, C. 2012. Effect of NaCl priming duration and concentration on germination behavior of Tunisian safflower. Journal of Stress Physiology & Biochemistry, 8(3): 30-36.
5. Bailly, C., Benamar, A., Corbineau, F. and Côme, D. 2000. Antioxidant systems in sunflower (Helianthus annuus L.) seeds as affected by priming. Seed Science Research, 10(1): 35-42. [DOI:10.1017/S0960258500000040]
6. Balouchi, H., Baladi, S., Moradi, A. and Dehnavi, M.M. 2017. The influence of temperature and moisture content on seed longevity of two genotypes of Linum usitatissimum. Seed Science and Technology, 45(1): 130-138. [DOI:10.15258/sst.2017.45.1.08]
7. Basra, S., Ashraf, M., Iqbal, N., Khaliq, A. and Ahmad, R. 2004. Physiological and biochemical aspects of pre-sowing heat stress on cottonseed. Seed Science and Technology, 32(3): 765-774. [DOI:10.15258/sst.2004.32.3.12]
8. Basra, S.M., Ullah, E., Warriach, E., Cheema, M. and Afzal, I. 2003. Effect of storage on growth and yield of primed canola (Brassica napus) seeds. International Journal of Agriculture and Biology, 5(2): 117-120.
9. Bewley, J.D., Bradford, K. and Hilhorst, H. 2012. Seeds: Physiology of Development, Germination and Dormancy. Springer Science & Business Media. [DOI:10.1007/978-1-4614-4693-4_4]
10. Biabani, A., Carpenter Boggs, L., Katozi, M. and Sabouri, H. 2011. Effects of seed deterioration and inoculation with 'Mesorhizobium ciceri' on yield and plant performance of chickpea. Australian Journal of Crop Science, 5(1): 66-70.
11. Bruggink, G., Ooms, J. and Van Der Toorn, P. 1999. Induction of longevity in primed seeds. Seed Science Research, 9(1): 49-53. [DOI:10.1017/S0960258599000057]
12. Bujalski, W. and Nienow, A. 1991. Large-scale osmotic priming of onion seeds: a comparison of different strategies for oxygenation. Scientia Horticulturae, 46(1-2): 13-24. [DOI:10.1016/0304-4238(91)90088-G]
13. Burgass, R. and Powell, A.A. 1984. Evidence for repair processes in the invigoration of seeds by hydration. Annals of Botany, 53: 753-757. [DOI:10.1093/oxfordjournals.aob.a086741]
14. Butler, L., Hay, F., Ellis, R., Smith, R. and Murray, T. 2009. Priming and re-drying improve the survival of mature seeds of Digitalis purpurea during storage. Annals of Botany, 103(8): 1261-1270. [DOI:10.1093/aob/mcp059] [PMID] [PMCID]
15. Chang, S. and Sung, J. 1998. Deteriorative changes in primed sweet corn seeds during storage. Seed Science and Technology, 26: 613-625.
16. Chiu, K., Chen, C., and Sung, J. 2002. Effect of priming temperature on storability of primed sh-2 sweet corn seed. Crop Science, 42: 1996-2003. [DOI:10.2135/cropsci2002.1996]
17. Damavandi, A., Latifi, N. and Dashtban, A. 2008. Evaluation of seed vigour test and it's field efficiency in forage sorghum (Sorghum bicolor L.). Journal of Agricultural Science and Natural Resources, 14: 17-24. [In Persian with English Summery].
18. Dearman, J., Brocklehurst, P. and Drew, R. 1986. Effects of osmotic priming and ageing on onion seed germination. Annals of Applied Biology, 108(3): 639-648. [DOI:10.1111/j.1744-7348.1986.tb02003.x]
19. Dell'aquila, A. and Bewley, J.D. 1989. Protein synthesis in the axes of polyethylene glycol-treated pea seed and during subsequent germination. Journal of Experimental Botany, 1001-1007. [DOI:10.1093/jxb/40.9.1001]
20. Farhoudi, R., Sharifzadeh, F., Poustini, K., Makkizadeh, M. and Kochak Por, M. 2007. The effects of NaCl priming on salt tolerance in canola (Brassica napus) seedlings grown under saline conditions. Seed Science and Technology, 35(3): 754-759. [DOI:10.15258/sst.2007.35.3.23]
21. Ghaderi-Far, F., Soltani, A. and Sadeghipour, H.R. 2010. Determination of seed viability constants in medicinal pumpkin (Cucurbita pepo L. subsp. Pepo. Convar. Pepo var. styriaca Greb), borago (Borago officinalis L.) and black cumin (Nigella sativa L.). Journal of Plant Production, 17: 53-66. [In Persian Whit English Summery].
22. Gurusinghe, S., Powell, A.L. and Bradford, K.J. 2002. Enhanced expression of BiP is associated with treatments that extend storage longevity of primed tomato seeds. Journal of the American Society for Horticultural Science, 127: 528-534. [DOI:10.21273/JASHS.127.4.528]
23. Hacisalihoglu, G., Paine, D., Hilderbrand, M., Khan, A. and Taylor, A. 1999. Embryo elongation and germination rates as sensitive indicators of lettuce seed quality: Priming and aging studies. HortScience, 34(7): 1240-1243. [DOI:10.21273/HORTSCI.34.7.1240]
24. Hampton, J.G. and Tekrony, D.M. 1995. Handbook of vigour test methods. The International Seed Testing Association, Zurich (Switzerland).
25. Hill, H., Cunningham, J.D., Bradford, K.J. and Taylor, A. 2007. Primed lettuce seeds exhibit increased sensitivity to moisture content during controlled deterioration. HortScience, 42(6): 1436-1439. [DOI:10.21273/HORTSCI.42.6.1436]
26. Hussain, S., Khan, F., Hussain, H.A. and Nie, L. 2016. Physiological and biochemical mechanisms of seed priming-induced chilling tolerance in rice cultivars. Frontiers in Plant Science, 7: 116. [DOI:10.3389/fpls.2016.00116]
27. Hussain, S., Zheng, M., Khan, F., Khaliq, A., Fahad, S., Peng, S., Huang, J., Cui, K. and Nie, L. 2015. Benefits of rice seed priming are offset permanently by prolonged storage and the storage conditions. Scientific Reports, 5: 8101. [DOI:10.1038/srep08101] [PMID] [PMCID]
28. Ibrahim, E.A. 2016. Seed priming to alleviate salinity stress in germinating seeds. Journal of Plant Physiology, 192: 38-46. [DOI:10.1016/j.jplph.2015.12.011] [PMID]
29. Jafar, M., Farooq, M., Cheema, M., Afzal, I., Basra, S., Wahid, M., Aziz, T. and Shahid, M. 2012. Improving the performance of wheat by seed priming under saline conditions. Journal of Agronomy and Crop Science, 198(1): 38-45. [DOI:10.1111/j.1439-037X.2011.00485.x]
30. Jatoi, S.A., Afzal, M., Nasim, S. and Anwar, R. 2001. Seed deterioration study in pea, using accelerated ageing techniques. Pakistan Journal of Biological Sciences, 4: 1490-1494. [DOI:10.3923/pjbs.2001.1490.1494]
31. Joao Abba, E. and Lovato, A. 1999. Effect of seed storage temperature and relative humidity on maize (Zea mays L.) seed viability and vigour. Seed Science and Technology, 27(1): 101-114.
32. Kapoor, N., Arya, A., Siddiqui, M.A., Kumar, H. and Amir, A. 2011. Physiological and biochemical changes during seed deterioration in aged seeds of rice (Oryza sativa L.). American Journal of Plant Physiology, 6: 28-35. [DOI:10.3923/ajpp.2011.28.35]
33. Khatun, A., Kabir, G. and Bhuiyan, M. 2009. Effect of harvesting stages on the seed quality of lentil (Lens culinaris L.) during storage. Bangladesh Journal of Agricultural Research, 34(4): 565-576. [DOI:10.3329/bjar.v34i4.5833]
34. Kibinza, S., Vinel, D., Côme, D., Bailly, C. and Corbineau, F. 2006. Sunflower seed deterioration as related to moisture content during ageing, energy metabolism and active oxygen species scavenging. Physiologia Plantarum, 128(3): 496-506. [DOI:10.1111/j.1399-3054.2006.00771.x]
35. Ma, F., Cholewa, E., Mohamed, T., Peterson, C.A. and Gijzen, M. 2004. Cracks in the palisade cuticle of soybean seed coats correlate with their permeability to water. Annals of Botany, 94: 213-228. [DOI:10.1093/aob/mch133] [PMID] [PMCID]
36. Malek, M., Ghaderi-Far, F., Torabi, B., Sadeghipour, H.R. and Hay, F.R. 2019. The influence of seed priming on storability of rapeseed (Brassica napus) seeds. Seed Science and Technology, 47: 87-92. [DOI:10.15258/sst.2019.47.1.09]
37. Malik, C.P. and Jyoti. 2013. Seed deterioration: A review. International Journal of Life Science Biotechnology and Pharma Research, 2(3): 374-385.
38. Mcdonald, C., Floyd, C. and Waniska, R. 2004. Effect of accelerated aging on mazie, sorghun and sorghum meal. Journal of Cereal Science, 39: 351-301. [DOI:10.1016/j.jcs.2004.01.001]
39. Mcdonald, M. 1999. Seed deterioration: physiology, repair and assessment. Seed Science and Technology, 27: 177-237.
40. Paparella, S., Araújo, S., Rossi, G., Wijayasinghe, M., Carbonera, D. and Balestrazzi, A. 2015. Seed priming: state of the art and new perspectives. Plant Cell Reports, 34(8): 1281-1293. [DOI:10.1007/s00299-015-1784-y] [PMID]
41. Powell, A.A., Yule, L.J., Jing, H.C., Groot, S.P., Bino, R.J. and Pritchard, H.W. 2000. The influence of aerated hydration seed treatment on seed longevity as assessed by the viability equations. Journal of Experimental Botany, 51: 2031-2043. [DOI:10.1093/jexbot/51.353.2031] [PMID]
42. Quintero, M.F.C., Castillo, O.G., Sánchez, P.D., Guzman, A.J. and Guzmán, J.M. 2018. Relieving dormancy and improving germination of Piquín Chili Pepper (Capsicum annuum var. glabriusculum) by priming techniques. Journal Cogent Food and Agriculture, 4: 1-13. [DOI:10.1080/23311932.2018.1550275]
43. Rajjou, L. and Debeaujon, I. 2008. Seed longevity: survival and maintenance of high germination ability of dry seeds. Comptes Rendus Biologies, 331: 796-805. [DOI:10.1016/j.crvi.2008.07.021] [PMID]
44. Schwember, A.R. and Bradford, K.J. 2005. Drying rates following priming affect temperature sensitivity of germination and longevity of lettuce seeds. HortScience, 40(3): 778-781. [DOI:10.21273/HORTSCI.40.3.778]
45. Schwember, A.R. and Bradford, K.J. 2010. Quantitative trait loci associated with longevity of lettuce seeds under conventional and controlled deterioration storage conditions. Journal of Experimental Botany, 61: 4423-4436. [DOI:10.1093/jxb/erq248] [PMID] [PMCID]
46. Shelar, V., Shaikh, R. and Nikam, A. 2008. Soybean seed quality during storage: a review. Agricultural Review, 29: 125-131.
47. Soltani, E., Miri, A.A. and Ghaderi-Far, F. 2009. The effect of seed priming on emergence and yield of cotton at different sowing dates. Journal of Plant Production, 16: 163-174. [In Persian with English Summery].
48. Taghi Zoghi, S., Soltani, E., Alahdadi, I. and Sadeghi, R. 2018. The effects of different priming methods on the storability and germination under salinity stress in rapeseed (Brassica napus) line Karaj 3. Iranian Journal of Seed Research, 4(2): 79-91. [In Persian with English Summery] [DOI:10.29252/yujs.4.2.79]
49. Timple, S.E. and Hay, F.R. 2018. High-temperature drying of seeds of wild Oryza species intended for long-term storage. Seed Science and Technology, 46:107-112. [DOI:10.15258/sst.2018.46.1.10]
50. Welbaum, G.E. and Bradford, K.J. 1988. Water relations of seed development and germination in muskmelon (Cucumis melo L.): I. Water relations of seed and fruit development. Plant Physiology, 86: 406-411. [DOI:10.1104/pp.86.2.406] [PMID] [PMCID]
51. Welbaum, G.E. and Bradford, K.J. 1991. Water relations of seed development and germination in muskmelon (Cucumis melo L.) VI. Influence of priming on germination responses to temperature and water potential during seed development. Journal of Experimental Botany, 42: 393-399. [DOI:10.1093/jxb/42.3.393]

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